##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939636_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1761818 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.367281978047675 31.0 31.0 34.0 30.0 34.0 2 31.562159087942113 31.0 31.0 34.0 30.0 34.0 3 31.608741652088924 31.0 31.0 34.0 30.0 34.0 4 35.43474127293511 37.0 35.0 37.0 33.0 37.0 5 35.19050548921625 37.0 35.0 37.0 32.0 37.0 6 35.20215936038796 37.0 35.0 37.0 32.0 37.0 7 35.100645469622854 37.0 35.0 37.0 32.0 37.0 8 35.06282487748451 37.0 35.0 37.0 32.0 37.0 9 36.62805635996454 39.0 35.0 39.0 32.0 39.0 10-11 36.507730934750356 38.0 35.0 39.0 32.0 39.0 12-13 36.43668784176345 38.0 35.0 39.0 32.0 39.0 14-15 37.42354289716645 39.0 36.0 41.0 32.0 41.0 16-17 37.38711007606915 39.0 36.0 41.0 32.0 41.0 18-19 37.39356108292684 39.0 36.0 41.0 32.0 41.0 20-21 37.34240596928854 39.0 36.0 41.0 31.5 41.0 22-23 37.19732259518293 39.0 36.0 40.0 31.0 41.0 24-25 37.050976321050186 39.0 36.0 40.0 31.0 41.0 26-27 36.72146952749944 39.0 35.5 40.0 30.0 41.0 28-29 36.50533851964278 38.0 35.0 40.0 30.0 41.0 30-31 36.228623785203695 38.0 35.0 40.0 30.0 41.0 32-33 36.00466081059451 38.0 35.0 40.0 30.0 41.0 34-35 35.92895917739517 38.0 35.0 40.0 29.5 41.0 36-37 35.80057162544598 38.0 34.5 40.0 29.0 41.0 38-39 35.80431435029044 38.0 35.0 40.0 29.0 41.0 40-41 35.65473930905462 38.0 34.5 40.0 28.5 41.0 42-43 35.43511872395446 38.0 34.0 40.0 27.0 41.0 44-45 35.16820409372591 38.0 34.0 40.0 26.5 41.0 46-47 34.79617048980087 38.0 33.5 40.0 25.5 41.0 48-49 34.4265687488719 38.0 33.0 40.0 24.0 41.0 50-51 34.15658030511665 38.0 33.0 40.0 24.0 41.0 52-53 33.887216216430986 37.5 33.0 40.0 23.0 41.0 54-55 33.592636696866535 37.0 33.0 40.0 22.5 41.0 56-57 32.84016368319543 36.5 31.5 39.5 18.0 41.0 58-59 29.779962799789764 34.5 25.5 38.5 7.5 40.0 60-61 29.4904178524683 34.0 25.0 38.0 8.0 40.0 62-63 31.191905179763175 35.0 29.5 38.0 8.5 40.0 64-65 31.19470342566599 35.0 30.0 38.5 4.5 40.0 66-67 26.428854172224373 31.0 18.0 36.5 2.0 39.5 68-69 19.02203462559697 18.5 7.5 31.5 2.0 36.5 70-71 17.13331797041465 13.5 7.0 28.5 2.0 34.5 72-73 18.77795578203878 18.0 7.5 30.5 2.0 35.0 74-75 20.203578065384733 22.0 7.5 31.5 2.0 35.5 76-77 22.08372658242792 27.0 11.0 33.0 2.0 35.5 78-79 24.266660915032087 30.5 12.5 34.5 2.0 36.0 80-81 25.813475909543435 31.5 20.5 35.0 2.0 36.0 82-83 26.827578671576745 32.5 25.5 35.0 2.0 36.0 84-85 26.841196707037845 33.0 25.5 35.0 2.0 36.0 86-87 26.546778100802694 33.0 25.0 35.0 2.0 35.5 88-89 26.08678308429134 33.0 24.5 35.0 2.0 35.0 90-91 24.335808806585018 31.5 11.0 34.5 2.0 35.0 92-93 22.798909705769837 31.0 2.0 34.0 2.0 35.0 94-95 19.070356302410353 25.5 2.0 34.0 2.0 35.0 96-97 16.448118647896663 11.5 2.0 32.0 2.0 35.0 98-99 16.652249267517984 16.0 2.0 32.5 2.0 35.0 100-101 15.87130367608913 2.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.20357554761357122 1101 2 0.15222483630005357 1101 3 0.1136472180720638 1101 4 0.161820454820635 1101 5 0.27111355584037256 1101 6 0.23967595608015557 1101 7 0.3253858689842275 1101 8 0.3962636639172885 1101 9 0.4624992612075829 1101 10-11 0.28562457540297004 1101 12-13 0.25653985991103667 1101 14-15 0.4007402210094355 1101 16-17 0.35239624668676583 1101 18-19 0.4383446811250167 1101 20-21 0.49865439370784514 1101 22-23 0.497965685425946 1101 24-25 0.4229873941105353 1101 26-27 0.38922496075607427 1101 28-29 0.2828648476401696 1101 30-31 0.24208930970163323 1101 32-33 0.4068705390019147 1101 34-35 0.26845783351890873 1101 36-37 0.13942143858776745 1101 38-39 0.0055301304225778836 1101 40-41 0.3025244976103636 1101 42-43 0.22999476981186717 1101 44-45 -0.01153596186401984 1101 46-47 0.07182506707478353 1101 48-49 0.04850323769132814 1101 50-51 -0.09941368184226462 1101 52-53 -0.1427310945310083 1101 54-55 -0.08736037535125973 1101 56-57 -3.6362802038696245 1101 58-59 -10.346166537705628 1101 60-61 -11.86858718072192 1101 62-63 -4.251876308350749 1101 64-65 -1.4440401853797376 1101 66-67 -2.6061101455111846 1101 68-69 -4.6864579230733945 1101 70-71 -4.596688441326174 1101 72-73 -3.7921478086828166 1101 74-75 -6.417305329722463 1101 76-77 -5.12677585335841 1101 78-79 0.6584165635641099 1101 80-81 1.3714345954761278 1101 82-83 0.018097366510488655 1101 84-85 -0.20495516392896462 1101 86-87 -0.1469462681329219 1101 88-89 -0.48251269951965625 1101 90-91 0.768316844343996 1101 92-93 -0.08127567336596897 1101 94-95 -8.714589227646655 1101 96-97 -9.245788655246121 1101 98-99 -8.358688483067892 1101 100-101 -9.1790370816941 1102 1 0.2988522268356739 1102 2 0.3556721202270374 1102 3 0.2685084215555733 1102 4 0.20766753587079023 1102 5 0.4303199570585008 1102 6 0.29878167588736204 1102 7 0.27767439746754974 1102 8 0.31008886541528824 1102 9 0.28977927340614684 1102 10-11 0.36562331012638083 1102 12-13 0.334747471252669 1102 14-15 0.4146051381338225 1102 16-17 0.5026402720398764 1102 18-19 0.25117593756630185 1102 20-21 0.33824787223436914 1102 22-23 0.3084386068847138 1102 24-25 0.21792201607941308 1102 26-27 -0.010205286275663639 1102 28-29 0.07606093090618771 1102 30-31 0.16676655602719137 1102 32-33 0.10650293289441493 1102 34-35 0.006236153815571299 1102 36-37 -0.06267716322752648 1102 38-39 -0.044176179414463945 1102 40-41 0.004914545615179122 1102 42-43 0.12824963436779768 1102 44-45 -0.10290839765123394 1102 46-47 -0.18710121596433993 1102 48-49 -0.04320741626202107 1102 50-51 -0.18884197148029358 1102 52-53 -0.2529157329917382 1102 54-55 -0.6059250650502932 1102 56-57 -3.687011441818406 1102 58-59 -9.777411461729553 1102 60-61 -8.061730300807483 1102 62-63 -2.943204107991498 1102 64-65 -1.3094486858755765 1102 66-67 -2.1795929761403947 1102 68-69 -5.0524817589378195 1102 70-71 -4.700301346714847 1102 72-73 -3.6219591851415327 1102 74-75 -4.498214766198549 1102 76-77 -4.5524841108562555 1102 78-79 1.065696256739944 1102 80-81 1.3680448437839559 1102 82-83 0.07777800312846139 1102 84-85 -0.23916048371410525 1102 86-87 -0.34463046050254675 1102 88-89 -0.4525364586023386 1102 90-91 0.7311139275029461 1102 92-93 -0.08146789807909371 1102 94-95 -8.833376124788321 1102 96-97 -9.735603854570442 1102 98-99 -8.952306784807162 1102 100-101 -9.431741340052765 1103 1 0.24679664125867973 1103 2 0.2562485318527514 1103 3 0.30211618615923896 1103 4 0.2547770011006705 1103 5 0.2139346089334282 1103 6 0.2719901906738045 1103 7 0.3124355151127389 1103 8 0.4266601698644621 1103 9 0.39353336603464584 1103 10-11 0.3071201790669065 1103 12-13 0.3476717006729686 1103 14-15 0.568445851443208 1103 16-17 0.4104928208600356 1103 18-19 0.3900227964408174 1103 20-21 0.5136009773341286 1103 22-23 0.3900750225368341 1103 24-25 0.46538908962487113 1103 26-27 0.5458719359289006 1103 28-29 0.33058242449042297 1103 30-31 0.39374797099058156 1103 32-33 0.37660555323623157 1103 34-35 0.21092241212002705 1103 36-37 0.22036293128702766 1103 38-39 0.2608486147620823 1103 40-41 0.3700503837960909 1103 42-43 0.2516486086941683 1103 44-45 0.306891659566098 1103 46-47 0.34872875320187546 1103 48-49 0.38491172293502984 1103 50-51 0.17791634887318253 1103 52-53 0.23498253671380098 1103 54-55 0.3198481635965251 1103 56-57 0.6477414640802763 1103 58-59 -7.77617122311209 1103 60-61 -7.3312673702205835 1103 62-63 -1.9246961558421383 1103 64-65 -0.07768962381596012 1103 66-67 -1.4154051973383552 1103 68-69 -3.1743157333171723 1103 70-71 -3.5530615531210685 1103 72-73 -2.5999543534558462 1103 74-75 -3.2548469435298912 1103 76-77 -0.27020135128559986 1103 78-79 3.486542702378184 1103 80-81 2.9622748998357515 1103 82-83 1.30340368523321 1103 84-85 0.8618617462783718 1103 86-87 0.9080267017297778 1103 88-89 0.6776324384806358 1103 90-91 1.9377151121136045 1103 92-93 1.5549286815960421 1103 94-95 -6.903090938779018 1103 96-97 -8.488248301840434 1103 98-99 -7.715875238985616 1103 100-101 -8.952961287709504 1104 1 0.2175046533906162 1104 2 0.21536275317631137 1104 3 0.2712766394218029 1104 4 0.20014222288759953 1104 5 0.35584928520454184 1104 6 0.1894651264335181 1104 7 0.3169749860255848 1104 8 0.2914875636047256 1104 9 0.27138995224299833 1104 10-11 0.2123425978714053 1104 12-13 0.29463792713576 1104 14-15 0.46053323928600065 1104 16-17 0.26118340386690164 1104 18-19 0.3589836835885478 1104 20-21 0.41508929824065177 1104 22-23 0.33346011668569986 1104 24-25 0.35065463366632343 1104 26-27 0.26055478903981566 1104 28-29 0.31181926049868025 1104 30-31 0.4010753120748731 1104 32-33 0.39294022403112194 1104 34-35 0.39021571638726726 1104 36-37 0.17365787842043545 1104 38-39 0.354542272877417 1104 40-41 0.3521326113541221 1104 42-43 0.3660560710978089 1104 44-45 0.39079920621928466 1104 46-47 0.31507158567165305 1104 48-49 0.30384075322274384 1104 50-51 0.30177476750917975 1104 52-53 0.3525461977778761 1104 54-55 0.20649855956938978 1104 56-57 0.8106848273221985 1104 58-59 -7.786278799252621 1104 60-61 -7.412584769858309 1104 62-63 -2.2419580982154486 1104 64-65 -0.34209682293976584 1104 66-67 -1.8150797834624548 1104 68-69 -3.209461221753923 1104 70-71 -3.816195530963437 1104 72-73 -2.8750594021099616 1104 74-75 -3.5114339417185256 1104 76-77 -0.2517428020628003 1104 78-79 3.4125184174462113 1104 80-81 2.747161320914987 1104 82-83 1.3560478046148177 1104 84-85 1.02782914170362 1104 86-87 0.9570709959291968 1104 88-89 0.9686503836341807 1104 90-91 2.121633716519856 1104 92-93 1.400396845798408 1104 94-95 -6.027450642220613 1104 96-97 -7.3873149748258164 1104 98-99 -5.3883166855503415 1104 100-101 -4.993313924720329 1105 1 0.2784028588752605 1105 2 0.2645386872931681 1105 3 0.32750478719550813 1105 4 0.23146093892450637 1105 5 0.3750745053064861 1105 6 0.2813166155442701 1105 7 0.2569951006049678 1105 8 0.23702286416498453 1105 9 0.3272121225113338 1105 10-11 0.17784556766794424 1105 12-13 0.29689449860043027 1105 14-15 0.373109063878438 1105 16-17 0.38899023018645806 1105 18-19 0.462302589186919 1105 20-21 0.4343263659351635 1105 22-23 0.38333927124149625 1105 24-25 0.424974581852851 1105 26-27 0.3564936975869344 1105 28-29 0.5303233571329073 1105 30-31 0.4997065202133797 1105 32-33 0.19499933561965577 1105 34-35 0.37387577999567867 1105 36-37 0.5343525685927233 1105 38-39 0.5367553505133813 1105 40-41 0.5231591920862471 1105 42-43 0.5544983496268117 1105 44-45 0.5833165300324126 1105 46-47 0.45442823506716223 1105 48-49 0.27506716334954007 1105 50-51 0.4141857789249883 1105 52-53 0.5101120703925659 1105 54-55 0.3097445366535183 1105 56-57 0.8433373190025506 1105 58-59 -8.227466559899657 1105 60-61 -7.816500530842344 1105 62-63 -2.2506028915934344 1105 64-65 -0.16758599687295472 1105 66-67 -1.4141098605507878 1105 68-69 -3.2670618473068096 1105 70-71 -3.538294713742829 1105 72-73 -2.5800061669273493 1105 74-75 -3.345779586016935 1105 76-77 -0.015020004135337217 1105 78-79 3.7987188681812896 1105 80-81 3.156834485327977 1105 82-83 1.7228337370466846 1105 84-85 1.4582348032731929 1105 86-87 1.36035830794739 1105 88-89 1.0750417649055066 1105 90-91 2.289269516258681 1105 92-93 1.8308354173473376 1105 94-95 -6.250500265203888 1105 96-97 -7.627885607539914 1105 98-99 -5.625201663856084 1105 100-101 -4.8648783861551 1106 1 0.0765854660705898 1106 2 0.14219458519291805 1106 3 0.10561085057420527 1106 4 0.13051250666107705 1106 5 0.1435508964537533 1106 6 0.22345754191267275 1106 7 0.21607552912485772 1106 8 0.1794215507902095 1106 9 0.08884819430775792 1106 10-11 0.17410516832253364 1106 12-13 0.20031570395641296 1106 14-15 0.22544642133910742 1106 16-17 0.3807152371140887 1106 18-19 0.2774249599538763 1106 20-21 0.1586658879545766 1106 22-23 0.23550304573002734 1106 24-25 0.19970231277832795 1106 26-27 0.3246942904878054 1106 28-29 0.400870980039592 1106 30-31 0.4041156322737365 1106 32-33 0.3399549726221025 1106 34-35 0.45096648755542645 1106 36-37 0.688701554021165 1106 38-39 0.6915968514832471 1106 40-41 0.47150780106969137 1106 42-43 0.5189357996302135 1106 44-45 0.5212519906285635 1106 46-47 0.440218743424758 1106 48-49 0.33607112125044125 1106 50-51 0.4502379688414919 1106 52-53 0.5277887773353598 1106 54-55 0.505203930263356 1106 56-57 1.162598493406385 1106 58-59 -7.951676678950388 1106 60-61 -7.305067059653457 1106 62-63 -1.6559792581672106 1106 64-65 0.14373244945487684 1106 66-67 0.590143445106861 1106 68-69 -2.1412105326249318 1106 70-71 -2.696099934825842 1106 72-73 -0.9145774117577652 1106 74-75 -0.7006365107749915 1106 76-77 2.287674453234878 1106 78-79 4.532278678494837 1106 80-81 3.666297110090735 1106 82-83 2.2104936608165673 1106 84-85 1.682649352872435 1106 86-87 1.5536398103812132 1106 88-89 1.4088530613925414 1106 90-91 2.6593478900162815 1106 92-93 2.0513548068790293 1106 94-95 -6.403986825594554 1106 96-97 -7.260021723777765 1106 98-99 -5.218609955057204 1106 100-101 -4.730452490921836 1107 1 0.06315782214339904 1107 2 0.1853686487220827 1107 3 0.06766180408480693 1107 4 0.05228564543744341 1107 5 0.035250124598334764 1107 6 0.059339505958249106 1107 7 0.08334955519723763 1107 8 0.09906292764436841 1107 9 0.1108136317232038 1107 10-11 0.06759664229826257 1107 12-13 0.05909798996660953 1107 14-15 0.1199881692815481 1107 16-17 0.20408576455886873 1107 18-19 0.17640625816925848 1107 20-21 0.1546602632961438 1107 22-23 0.20025221830347562 1107 24-25 0.13583465395029748 1107 26-27 0.11554240014817907 1107 28-29 0.01147160882236875 1107 30-31 0.034409985304968416 1107 32-33 -0.015059887283371154 1107 34-35 0.05308995986093379 1107 36-37 0.02182747636895499 1107 38-39 0.16148840078190574 1107 40-41 -0.08732672999386182 1107 42-43 -0.08819521520308626 1107 44-45 -0.0773900985185989 1107 46-47 0.004008367280846414 1107 48-49 -0.20878158965889781 1107 50-51 0.005230876613879332 1107 52-53 -0.030012366017700742 1107 54-55 0.0436521447208591 1107 56-57 0.3578440400400851 1107 58-59 -8.186353936540506 1107 60-61 -7.6307678904572604 1107 62-63 -2.051772635889222 1107 64-65 -0.401657295572889 1107 66-67 0.49441781709164445 1107 68-69 -2.638391551244844 1107 70-71 -2.686410038128793 1107 72-73 -0.987646289261793 1107 74-75 -0.741294777979018 1107 76-77 2.273738942359106 1107 78-79 4.128016075718943 1107 80-81 3.1007873907710213 1107 82-83 1.7008408627127451 1107 84-85 1.2333280780317253 1107 86-87 1.1125186039042596 1107 88-89 1.2096648297826995 1107 90-91 2.1169610842205095 1107 92-93 1.7598504358971887 1107 94-95 -6.236563177057928 1107 96-97 -7.703448671786518 1107 98-99 -5.416517389717626 1107 100-101 -4.7662070916590995 1108 1 0.07410951653700693 1108 2 -0.013176771271883325 1108 3 -0.0685764892520524 1108 4 -0.09233579608810771 1108 5 0.004129094122795607 1108 6 0.02022262316327783 1108 7 0.042387078486626706 1108 8 0.01728256468101108 1108 9 0.03241668547367027 1108 10-11 -0.05474547524965345 1108 12-13 -0.10761016039094073 1108 14-15 -0.15145511201298945 1108 16-17 -0.0939100051374524 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0.4583150997686545 2311 86-87 0.5643469775103149 2311 88-89 0.6209278118854975 2311 90-91 1.947758451296984 2311 92-93 2.4288486675768475 2311 94-95 -1.2433528308992372 2311 96-97 -5.977913593614881 2311 98-99 -5.278896348855808 2311 100-101 -5.819145477931118 2312 1 -0.18827087527260034 2312 2 -0.19935222613460368 2312 3 -0.05226940678034708 2312 4 -0.014505958760452131 2312 5 -0.14633996271095384 2312 6 -0.17001052490866897 2312 7 -0.0863214916322903 2312 8 -0.04572497252319607 2312 9 -0.06984603844171744 2312 10-11 -0.236735750328954 2312 12-13 -0.23748460109080582 2312 14-15 -0.24122975116999612 2312 16-17 -0.2828376854808141 2312 18-19 -0.2121729816226079 2312 20-21 -0.3457983309361481 2312 22-23 -0.3782511770576704 2312 24-25 -0.2167206860267754 2312 26-27 -0.23132019796452852 2312 28-29 -0.16890805428725741 2312 30-31 -0.0450458750001701 2312 32-33 -0.3151811501813384 2312 34-35 -0.362846189711334 2312 36-37 -0.296569207641987 2312 38-39 -0.35656654233477525 2312 40-41 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2313 62-63 0.8151380939199413 2313 64-65 0.060406495460764376 2313 66-67 -1.638119853148865 2313 68-69 -4.654256517921169 2313 70-71 -2.90924348185111 2313 72-73 -2.681670278167701 2313 74-75 -3.42287723854945 2313 76-77 0.5832061775290356 2313 78-79 3.0870476193708853 2313 80-81 2.4794566170822314 2313 82-83 1.1124948346463412 2313 84-85 0.8102333228882905 2313 86-87 0.7330478500854909 2313 88-89 0.9349458042414476 2313 90-91 2.4904749725344537 2313 92-93 3.6434147236812677 2313 94-95 5.728283968696925 2313 96-97 1.1574969133440849 2313 98-99 2.8893484048760723 2313 100-101 4.106834366303394 2314 1 -0.3137353754539447 2314 2 -0.327879356050115 2314 3 -0.3272160409524112 2314 4 -0.25005783837517725 2314 5 -0.20790947345840038 2314 6 -0.362648918058575 2314 7 -0.35421379905692874 2314 8 -0.4364953274879255 2314 9 -0.3884189525571955 2314 10-11 -0.45674107687428744 2314 12-13 -0.6558943730263351 2314 14-15 -0.8049523639609291 2314 16-17 -0.4711552867688269 2314 18-19 -0.5175949497409604 2314 20-21 -0.6049571115052359 2314 22-23 -0.6102080400704324 2314 24-25 -0.4818120068360301 2314 26-27 -0.6970626153328183 2314 28-29 -0.45750603528566813 2314 30-31 -0.476979451106196 2314 32-33 -0.6240869198470662 2314 34-35 -0.5523819371206642 2314 36-37 -0.6211034360490686 2314 38-39 -0.49082305430410855 2314 40-41 -0.6904514016131742 2314 42-43 -0.6379782583938365 2314 44-45 -0.5807666480774074 2314 46-47 -0.6396968601682218 2314 48-49 -0.5921820320639739 2314 50-51 -0.5268856835380262 2314 52-53 -0.7066358005373985 2314 54-55 -0.5500963626396427 2314 56-57 0.3464142691927563 2314 58-59 3.4413566967844424 2314 60-61 3.4432048020680277 2314 62-63 0.9228631070020974 2314 64-65 0.004820414874682655 2314 66-67 -1.683573803959959 2314 68-69 -4.593162849684642 2314 70-71 -3.29616786698978 2314 72-73 -2.5468652022911957 2314 74-75 -3.794233858120357 2314 76-77 0.5784835192349504 2314 78-79 3.21984458431071 2314 80-81 2.5855986892957326 2314 82-83 1.1127695599210696 2314 84-85 0.6050266248244469 2314 86-87 0.6692723398814096 2314 88-89 0.8895049355862916 2314 90-91 2.4425681174847433 2314 92-93 4.029057057537887 2314 94-95 6.347364293134394 2314 96-97 3.530815856305887 2314 98-99 5.62108571518481 2314 100-101 6.008816312928202 2315 1 -0.38932766692939325 2315 2 -0.46097461922297 2315 3 -0.5059383531754094 2315 4 -0.21791084342580547 2315 5 -0.470569907501762 2315 6 -0.39723680088926727 2315 7 -0.6369864805060175 2315 8 -0.637223247065684 2315 9 -0.5964101854229185 2315 10-11 -0.48247739745314533 2315 12-13 -0.5897300109925041 2315 14-15 -0.7229147074139135 2315 16-17 -0.6408814224922281 2315 18-19 -0.6037637254350727 2315 20-21 -0.6347050145986444 2315 22-23 -0.7207547655190325 2315 24-25 -0.5651800308704154 2315 26-27 -0.5974325937493532 2315 28-29 -0.5937050713021463 2315 30-31 -0.6360969196714095 2315 32-33 -0.5612850480538611 2315 34-35 -0.5764936461935761 2315 36-37 -0.6336025374345269 2315 38-39 -0.7635086829915636 2315 40-41 -0.6132236316651785 2315 42-43 -0.6097801519209725 2315 44-45 -0.5167836206073915 2315 46-47 -0.44617708312494386 2315 48-49 -0.4104545856172237 2315 50-51 -0.3352580125630311 2315 52-53 -0.3381495021104328 2315 54-55 -0.195261064057199 2315 56-57 0.3964386780715685 2315 58-59 3.422897900142164 2315 60-61 3.6578545597346626 2315 62-63 0.9232288222240435 2315 64-65 0.23548473762025068 2315 66-67 -1.7926668636463567 2315 68-69 -4.825601413706959 2315 70-71 -3.5014097573069787 2315 72-73 -2.832604565620702 2315 74-75 -0.37489224583032055 2315 76-77 2.0249478262652083 2315 78-79 3.828849036423332 2315 80-81 2.81378074609486 2315 82-83 0.9789349250595052 2315 84-85 0.8430646023649402 2315 86-87 0.9899728245481221 2315 88-89 0.9626274999229167 2315 90-91 2.3177597522610434 2315 92-93 3.7887324194496728 2315 94-95 6.1633497681796605 2315 96-97 3.6082133181168548 2315 98-99 5.754529557209208 2315 100-101 6.07125999278402 2316 1 -0.5454885656725992 2316 2 -0.4082223507716414 2316 3 -0.5772692336925864 2316 4 -0.42420081196126347 2316 5 -0.505438355487243 2316 6 -0.6445177440530045 2316 7 -0.5678610588021087 2316 8 -0.5524719334482882 2316 9 -0.7147447267134268 2316 10-11 -0.5919857790615239 2316 12-13 -0.5330345839897319 2316 14-15 -0.6796751738295299 2316 16-17 -0.7079311182542227 2316 18-19 -0.7400736209395617 2316 20-21 -0.6435202190605267 2316 22-23 -0.9707489996136331 2316 24-25 -0.7859689451100706 2316 26-27 -0.8401901841313588 2316 28-29 -0.7881328426701586 2316 30-31 -0.808149518566772 2316 32-33 -0.7845472474123483 2316 34-35 -0.8137974108108708 2316 36-37 -0.9130968732973557 2316 38-39 -1.1285423875915441 2316 40-41 -0.8601342595549823 2316 42-43 -0.8370695479020185 2316 44-45 -0.8725215325308753 2316 46-47 -1.0367940522998396 2316 48-49 -1.2302899344227853 2316 50-51 -0.9868106555675737 2316 52-53 -1.0156774047681125 2316 54-55 -0.840996196814288 2316 56-57 -0.08573559295943056 2316 58-59 3.008622210294835 2316 60-61 3.0240842572610376 2316 62-63 0.4934190625066215 2316 64-65 -0.1830763172636729 2316 66-67 -2.235740512132544 2316 68-69 -3.3267563245574134 2316 70-71 -3.48442455319681 2316 72-73 -0.891832234124962 2316 74-75 5.19059013269791 2316 76-77 5.254246640276129 2316 78-79 4.349669576654069 2316 80-81 2.7640232889052676 2316 82-83 1.10655387136336 2316 84-85 0.6074836902794267 2316 86-87 0.5999155229803144 2316 88-89 0.7384204128179626 2316 90-91 2.073304823478626 2316 92-93 3.652096894438042 2316 94-95 6.165126531926877 2316 96-97 3.646089694798844 2316 98-99 5.596933236318128 2316 100-101 5.615454936892219 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 16.0 4 34.0 5 89.0 6 258.0 7 635.0 8 1321.0 9 2125.0 10 3638.0 11 5423.0 12 8016.0 13 10869.0 14 13124.0 15 17134.0 16 22550.0 17 28886.0 18 29483.0 19 27749.0 20 28875.0 21 29267.0 22 29313.0 23 29973.0 24 31272.0 25 33361.0 26 41822.0 27 58309.0 28 80560.0 29 104360.0 30 124777.0 31 138259.0 32 143308.0 33 142677.0 34 144399.0 35 150390.0 36 142968.0 37 100279.0 38 34600.0 39 1696.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.80405094682195 20.27432118026577 14.308213057365704 22.61341481554657 2 15.430757746486867 22.30074278853659 39.35967484197649 22.908824623000047 3 18.684399924623058 27.518656928208003 31.038669279128534 22.758273868040405 4 12.422111705068287 17.493407378060617 38.15575729161582 31.92872362525528 5 12.195385137003923 39.66250357084239 34.52264865352975 13.619462638623936 6 29.743821438990857 37.929343439560725 18.078882154683402 14.247952966765013 7 26.402159587426173 34.40321304470723 22.108242735628767 17.086384632237834 8 25.673225807916378 34.96233431868027 20.65510813842081 18.70933173498254 9 26.843294823869435 15.950171924682346 20.84591030401551 36.36062294743271 10-11 24.24992252321182 27.065167911782034 28.166274836560873 20.518634728445274 12-13 25.751609984686276 24.703090784632693 28.625998826212466 20.919300404468565 14-15 22.717498438397072 25.532725833609327 27.494655358987043 24.25512036900656 16-17 20.949956507981106 28.977632473283983 28.064512924863106 22.007898093871813 18-19 20.971738463507588 27.38686857248917 30.794384075708443 20.847008888294805 20-21 22.203712301724696 26.458465062793092 30.330232748218034 21.007589887264178 22-23 21.660211213644086 26.6173066684527 30.14153561832153 21.58094649958168 24-25 21.56514118532843 26.71148028837299 30.5151702888299 21.20820823746868 26-27 21.498517579461403 26.57918867167911 30.677832427874783 21.2444613209847 28-29 21.231835009883344 26.736254489365297 30.78843968038415 21.243470820367207 30-31 21.238778817472546 26.95357133734642 30.551785597720425 21.2558642474606 32-33 21.21057744676467 27.000995328738036 30.21343514994782 21.57499207454947 34-35 21.552722335616608 26.825097797928933 30.23272842031654 21.38945144613792 36-37 21.88111921878506 26.5108061817779 30.332266301584642 21.275808297852397 38-39 21.747758448368266 26.71965899502029 30.34303571798811 21.189546838623336 40-41 21.529675683260173 26.843287147363558 30.39179207281292 21.23524509656335 42-43 21.24289783015805 27.098213106423685 29.968905682843644 21.689983380574613 44-45 21.35864416793661 27.181206051989847 29.68474603713398 21.77540374293956 46-47 21.655607525612698 27.23281174862247 29.01360755799339 22.097973167771446 48-49 21.690306052894666 27.29000045442152 29.021772471144235 21.99792102153958 50-51 21.88234224946715 27.55810964899202 28.91323702943219 21.646311072108645 52-53 21.80053658525504 27.66495112348958 28.831830955198996 21.702681336056393 54-55 21.49355660662903 27.56839912070239 28.87996315627181 22.058081116396767 56-57 21.604595724281534 27.936184001640356 28.494717404874383 21.964502869203727 58-59 20.902050043558027 27.077026091645408 30.1381248404278 21.882799024368765 60-61 21.01858459954172 27.46498407188923 29.787788467398492 21.728642861170552 62-63 21.951110834872274 27.236817944323306 28.97729288402141 21.83477833678301 64-65 21.69365746892477 27.52457325660003 28.8928539601867 21.888915314288504 66-67 21.677407467147617 27.172149191412025 29.40556026567768 21.74488307576268 68-69 21.555131590540523 26.574173668050516 30.705360889314598 21.165333852094367 70-71 22.459397008033825 26.470729305925822 29.59565754158553 21.47421614445482 72-73 21.517353067780938 26.771970631913412 29.599676681615517 22.110999618690137 74-75 21.426484703111136 27.273456581035067 29.32756257896592 21.972496136887877 76-77 20.1521123474187 27.55619429753125 29.75146633603572 22.54022701901433 78-79 21.26665214734241 27.433559105971185 28.98951497369538 22.31027377299103 80-81 21.164806804808904 28.065237357742216 28.255486959200386 22.51446887824849 82-83 21.316051538881343 27.83140459372732 28.093038118917672 22.759505748473664 84-85 21.221272527115122 27.67787484742111 27.944165987671088 23.15668663779268 86-87 21.16002811907298 27.54491374840468 28.320628949987615 22.97442918253473 88-89 21.46518539372398 27.279832536618425 28.134631386442866 23.120350683214724 90-91 21.128782399207214 27.294014796421067 28.517766526372146 23.05943627799958 92-93 20.738652901059652 27.592189632803247 28.36941561386257 23.29974185227453 94-95 20.021752058062805 27.35666640396065 29.146668768314786 23.474912769661753 96-97 19.99466568755477 26.978516114357497 29.115849258334286 23.910968939753445 98-99 20.44649871306673 27.0295409033386 28.643249527934024 23.88071085566065 100-101 19.75200588512866 27.181866632867713 29.1394655026581 23.92666197934553 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1235.0 1 988.0 2 746.5 3 859.5 4 954.0 5 1001.5 6 1299.5 7 1877.5 8 2547.0 9 2992.5 10 3071.5 11 3098.0 12 3161.5 13 3195.0 14 3333.5 15 3539.5 16 3777.0 17 3928.5 18 4121.5 19 4472.0 20 4844.5 21 5243.5 22 5937.0 23 6964.5 24 8007.5 25 9435.0 26 11497.0 27 14014.0 28 16646.5 29 19404.0 30 22300.0 31 25644.0 32 29685.0 33 33799.0 34 37636.0 35 41338.5 36 45770.0 37 49733.0 38 52402.5 39 54571.0 40 56140.0 41 57286.5 42 58323.5 43 59839.0 44 62174.5 45 64281.5 46 66433.0 47 69309.5 48 72588.0 49 71211.5 50 65623.0 51 61668.5 52 58668.5 53 55503.0 54 51935.0 55 47877.0 56 43097.5 57 38140.0 58 33591.0 59 29219.5 60 24486.0 61 19552.0 62 15075.0 63 11068.5 64 7772.5 65 5346.5 66 3640.0 67 2522.5 68 1699.5 69 1143.0 70 744.0 71 467.5 72 326.0 73 239.5 74 164.0 75 103.5 76 58.5 77 24.0 78 9.5 79 4.5 80 2.5 81 1.0 82 0.5 83 0.5 84 1.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025655317405089514 2 0.012089784529389528 3 3.4055731068702896E-4 4 0.0 5 0.058405578782825464 6 0.0 7 0.0 8 1.13519103562343E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 3.689370865776147E-4 16-17 2.837977589058575E-5 18-19 3.689370865776147E-4 20-21 0.0 22-23 0.0 24-25 1.4189887945292872E-4 26-27 1.9865843123410024E-4 28-29 8.797730526081582E-4 30-31 0.004711042797837234 32-33 0.005023220332633678 34-35 0.0012487101391857728 36-37 0.08417441529147733 38-39 0.07801600392322022 40-41 0.08349330067010327 42-43 0.08675697489752063 44-45 0.08914087607232984 46-47 0.08567854341367837 48-49 0.07594428028320746 50-51 0.07069402174344909 52-53 0.059824567577354756 54-55 0.04921053139427568 56-57 0.06953045093193508 58-59 0.7379593124829011 60-61 0.34719817824542604 62-63 0.003263674227417361 64-65 0.002554179830152717 66-67 1.1185604869515466 68-69 2.403000763983567 70-71 2.3713289340896733 72-73 1.830694203374015 74-75 1.3017803201011682 76-77 0.7516099846862729 78-79 0.5749458797673767 80-81 0.04032766154052235 82-83 1.4189887945292872E-4 84-85 0.0013906090186387016 86-87 0.0013906090186387016 88-89 0.0 90-91 0.33987619606565495 92-93 0.05701496976418677 94-95 0.9737384905818877 96-97 1.6821260765867985 98-99 0.6546079106922509 100-101 0.5837436102934582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1761818.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.54952004298335 #Duplication Level Percentage of deduplicated Percentage of total 1 71.56919360806164 33.315116123186215 2 14.079576087460188 13.107950185598744 3 5.426566638441174 7.578122175021068 4 2.727919113392037 5.0793330177792 5 1.5481148994897216 3.6032002771318976 6 0.9931597570594517 2.773866601027406 7 0.687881389248286 2.241438396120583 8 0.49328735273228735 1.836983161036945 9 0.3707637772493842 1.5532988292254184 >10 1.9142773524929273 15.839046902914777 >50 0.12328391459059862 3.9355826040760156 >100 0.0606106152836403 5.0388428719212 >500 0.0031705194765047 1.0515513894173507 >1k 0.0018291458518296347 1.6225963004855584 >5k 2.4388611357728466E-4 0.8081477533676017 >10k+ 1.2194305678864233E-4 0.614923411690049 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10833 0.6148762244454308 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7847 0.4453922028268527 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6390 0.36269353588168585 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4150 0.23555213989186172 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3599 0.20427762686043618 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2294 0.1302064117860074 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1969 0.11175955745712668 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1923 0.10914861807519277 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1855 0.10528896855407312 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1776 0.10080496396336057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.67595517811715E-5 7 0.0 0.0 0.0 0.0 5.67595517811715E-5 8 0.0 0.0 0.0 0.0 5.67595517811715E-5 9 0.0 0.0 0.0 0.0 5.67595517811715E-5 10-11 0.0 0.0 0.0 2.837977589058575E-5 5.67595517811715E-5 12-13 0.0 0.0 0.0 8.513932767175724E-5 5.67595517811715E-5 14-15 0.0 0.0 0.0 1.4189887945292872E-4 1.13519103562343E-4 16-17 0.0 0.0 0.0 3.9731686246820047E-4 2.27038207124686E-4 18-19 0.0 0.0 0.0 4.8245619013995774E-4 2.8379775890585745E-4 20-21 0.0 0.0 0.0 8.513932767175723E-4 2.8379775890585745E-4 22-23 0.0 0.0 0.0 0.0019582045364504165 3.121775347964432E-4 24-25 0.0 0.0 0.0 0.0032352944515267753 3.9731686246820047E-4 26-27 0.0 0.0 0.0 0.004285346159478448 3.9731686246820047E-4 28-29 0.0 0.0 0.0 0.007180083300318194 3.9731686246820047E-4 30-31 0.0 0.0 0.0 0.016431890240649148 3.9731686246820047E-4 32-33 0.0 0.0 0.0 0.03575851762213804 5.108359660305435E-4 34-35 0.0 0.0 0.0 0.06305986202888153 5.108359660305435E-4 36-37 0.0 0.0 0.0 0.10775800905655408 5.108359660305435E-4 38-39 0.0 0.0 0.0 0.17487617903778938 5.108359660305435E-4 40-41 0.0 0.0 0.0 0.25482200772156943 5.108359660305435E-4 42-43 0.0 0.0 0.0 0.3336894049215072 5.108359660305435E-4 44-45 0.0 0.0 0.0 0.42297217987328994 5.108359660305435E-4 46-47 0.0 0.0 0.0 0.5299355552049076 5.108359660305435E-4 48-49 0.0 5.67595517811715E-5 0.0 0.6429438228012201 5.108359660305435E-4 50-51 0.0 5.67595517811715E-5 0.0 0.7654593153208787 5.108359660305435E-4 52-53 0.0 5.67595517811715E-5 0.0 0.8781270256065041 5.959752937023007E-4 54-55 0.0 5.67595517811715E-5 0.0 0.9982586169513537 7.094943972646437E-4 56-57 0.0 5.67595517811715E-5 0.0 1.109762756425465 7.378741731552294E-4 58-59 0.0 5.67595517811715E-5 0.0 1.1977684414621714 7.378741731552294E-4 60-61 0.0 5.67595517811715E-5 0.0 1.2762101420237504 7.378741731552294E-4 62-63 0.0 5.67595517811715E-5 0.0 1.344690541247734 7.378741731552294E-4 64-65 2.837977589058575E-5 5.67595517811715E-5 0.0 1.4179955023731168 7.378741731552294E-4 66-67 5.67595517811715E-5 5.67595517811715E-5 0.0 1.479494476728016 7.378741731552294E-4 68-69 5.67595517811715E-5 5.67595517811715E-5 0.0 1.553480552474773 7.378741731552294E-4 70-71 5.67595517811715E-5 5.67595517811715E-5 0.0 1.6766771596158059 7.946337249364009E-4 72-73 5.67595517811715E-5 5.67595517811715E-5 0.0 1.8412231002294221 0.0010784314838422585 74-75 5.67595517811715E-5 5.67595517811715E-5 0.0 2.050523947422492 0.0010784314838422585 76-77 5.67595517811715E-5 5.67595517811715E-5 0.0 2.328021396080639 0.0011351910356234298 78-79 5.67595517811715E-5 5.67595517811715E-5 0.0 2.679249502502529 0.0011919505874046013 80-81 5.67595517811715E-5 5.67595517811715E-5 0.0 3.093622610280971 0.0011919505874046013 82-83 5.67595517811715E-5 5.67595517811715E-5 0.0 3.5197733250540066 0.0011919505874046013 84-85 5.67595517811715E-5 8.513932767175724E-5 0.0 3.8933930746535683 0.0011919505874046013 86-87 5.67595517811715E-5 1.13519103562343E-4 0.0 4.230970508872086 0.0011919505874046013 88-89 5.67595517811715E-5 1.13519103562343E-4 0.0 4.555862183267511 0.0011919505874046013 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10100 0.0 26.581154 1 TACATGG 10090 0.0 26.040438 2 ACATGGG 9985 0.0 24.753149 3 GTATCAA 14885 0.0 24.312492 1 GGTATCA 11040 0.0 23.719572 1 CATGGGA 6645 0.0 23.637228 4 ATGGGAG 2640 0.0 21.082657 5 AGTACTT 8690 0.0 20.585814 12-13 GAGTACT 8345 0.0 20.504837 12-13 TCAACGC 17730 0.0 20.325062 4 ATCAACG 17825 0.0 20.216738 3 TATCAAC 17930 0.0 20.152676 2 CAACGCA 17910 0.0 20.147125 5 AACGCAG 18125 0.0 19.921152 6 ATGGGAT 2740 0.0 19.452486 5 GTACTTT 9105 0.0 18.715626 14-15 ACGCAGA 19630 0.0 18.32179 7 CATGGGG 3710 0.0 18.307787 4 CGCAGAG 19645 0.0 18.211823 8 AGTACAT 7480 0.0 17.973288 2 >>END_MODULE