FastQCFastQC Report
Fri 27 May 2016
SRR939635_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939635_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693781
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT118950.4415726445468284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108740.4036705285247762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71650.2659830179216499No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59650.2214359667693847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52130.19351981471396523No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50130.18609530618858772No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC44480.1651210696043962No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42400.15739958073800359No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35980.1335669083715417No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33610.12476886576896934No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28790.1068758002228095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG116650.026.85141
TACATGG115800.026.3932652
ACATGGG118800.024.6881123
GAGTACT91600.022.06996712-13
CATGGGA83450.021.7250564
AGTACTT94600.020.9687612-13
CATGGGG40800.020.8217284
GTACTTT100800.019.93942814-15
GTGTAGC15600.018.5572241
ATGGGAG33650.018.4757485
GTATAGG6900.017.8826561
TAGCCCT18050.017.8795094
ATGGGAT37250.017.8368245
ACTTTTT119350.017.35889216-17
AGTACAT83050.017.3148542
GTCCTAG15250.017.1159341
TACTTTT116700.016.87707114-15
AGAGTAC196250.016.23752810-11
GTATCAA322050.016.018211
AGCACCG5601.8189894E-1215.2545815
Produced by FastQC (version 0.11.5)