##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939635_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2693781 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.9629691500534 31.0 30.0 33.0 26.0 34.0 2 30.083018255752787 31.0 30.0 33.0 25.0 34.0 3 30.119562800390973 31.0 30.0 34.0 25.0 34.0 4 33.68777083215005 35.0 33.0 37.0 28.0 37.0 5 33.693558236545584 35.0 33.0 37.0 28.0 37.0 6 33.799663372783456 35.0 33.0 37.0 28.0 37.0 7 33.59641708067582 35.0 33.0 37.0 28.0 37.0 8 33.60254452756182 35.0 33.0 37.0 28.0 37.0 9 34.81313365860105 37.0 34.0 39.0 27.0 39.0 10-11 34.705171652780976 37.0 34.0 39.0 27.0 39.0 12-13 34.54483827749917 37.0 33.5 39.0 27.0 39.0 14-15 35.16286736004152 38.0 33.0 40.0 26.5 41.0 16-17 34.90956707319563 38.0 33.0 40.0 25.5 41.0 18-19 34.906974806044 38.0 33.0 40.0 25.5 41.0 20-21 35.04452626252839 38.0 34.0 40.0 25.5 41.0 22-23 34.899505193629324 38.0 33.0 40.0 25.0 41.0 24-25 34.76249554065457 38.0 33.0 40.0 25.0 41.0 26-27 34.86856800905493 38.0 33.5 40.0 25.0 41.0 28-29 34.7492023293653 38.0 33.0 40.0 24.0 41.0 30-31 34.515791372795334 38.0 33.0 40.0 24.0 41.0 32-33 34.27210081294656 38.0 33.0 40.0 24.0 41.0 34-35 34.01504817949195 38.0 33.0 40.0 23.5 41.0 36-37 33.70967313229991 38.0 32.0 40.0 20.5 41.0 38-39 33.53218691497193 37.5 32.0 40.0 20.0 41.0 40-41 33.25406612490028 37.0 31.0 40.0 18.5 41.0 42-43 32.92439901387678 37.0 31.0 40.0 16.0 41.0 44-45 32.65259295391867 37.0 31.0 40.0 15.0 41.0 46-47 32.178339478970265 37.0 30.0 40.0 11.0 41.0 48-49 31.78741553229457 36.5 30.0 39.0 9.0 41.0 50-51 31.178652793230036 35.5 29.5 38.5 8.0 40.0 52-53 31.240764561038926 36.0 30.0 39.0 8.0 40.0 54-55 31.276051950771055 36.0 29.0 39.0 7.0 40.0 56-57 31.1191052650531 36.0 29.0 39.0 4.0 40.0 58-59 30.70922562004855 35.5 28.0 39.0 2.0 40.0 60-61 30.253765432305002 35.0 28.0 39.0 2.0 40.0 62-63 29.841498065358692 35.0 27.5 38.0 2.0 40.0 64-65 29.214342962549665 34.0 26.5 38.0 2.0 40.0 66-67 28.6455019914388 34.0 26.0 37.5 2.0 39.5 68-69 28.11191815518782 33.5 25.5 37.0 2.0 39.0 70-71 27.52322757492164 33.0 24.5 36.0 2.0 39.0 72-73 26.75637162041012 32.0 22.0 36.0 2.0 38.5 74-75 26.28304825076723 32.0 22.0 35.0 2.0 38.0 76-77 25.882526827533496 31.5 20.5 35.0 2.0 37.5 78-79 25.278823334190864 31.0 18.5 35.0 2.0 37.0 80-81 24.581672563582565 31.0 15.5 34.0 2.0 36.0 82-83 23.885108885985908 30.0 9.0 34.0 2.0 36.0 84-85 23.191366521628893 30.0 4.5 34.0 2.0 35.0 86-87 22.511557175583313 29.0 2.0 34.0 2.0 35.0 88-89 21.681096013373022 29.0 2.0 34.0 2.0 35.0 90-91 20.427195269400148 27.0 2.0 33.0 2.0 34.5 92-93 18.83279004492199 25.0 2.0 32.0 2.0 34.0 94-95 18.50638489171911 25.0 2.0 32.0 2.0 34.0 96-97 17.829952768989017 24.0 2.0 32.0 2.0 34.0 98-99 16.82843649873542 19.0 2.0 32.0 2.0 34.0 100-101 15.222206445141605 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.292471650092633 1101 2 1.202867931526665 1101 3 1.2387743983831925 1101 4 1.3937837860597213 1101 5 1.3459229562236743 1101 6 1.0919761308446354 1101 7 1.2372972530000226 1101 8 1.110988643304779 1101 9 1.4397664412202502 1101 10-11 1.322118225680292 1101 12-13 1.2733680501396236 1101 14-15 1.5647145134770213 1101 16-17 1.4602410383505529 1101 18-19 1.460148499763683 1101 20-21 1.5782315008702383 1101 22-23 1.6574823677257982 1101 24-25 1.5330093591512153 1101 26-27 1.7096739297827668 1101 28-29 1.7633002371176119 1101 30-31 1.8363314140335447 1101 32-33 1.8496115601210619 1101 34-35 1.6865927600176391 1101 36-37 1.8157238863626262 1101 38-39 1.6498454653907189 1101 40-41 1.7943385625027304 1101 42-43 1.7165051564615155 1101 44-45 1.7368058144607588 1101 46-47 1.8593257309563853 1101 48-49 1.7567449870748533 1101 50-51 1.8448632957598257 1101 52-53 1.918963274968906 1101 54-55 1.9920605338608048 1101 56-57 1.9786762343690505 1101 58-59 1.9678387089651785 1101 60-61 1.7330155917542704 1101 62-63 1.79482761676773 1101 64-65 1.566562891412893 1101 66-67 1.7245212297662782 1101 68-69 1.5066322102095242 1101 70-71 1.4528148853465161 1101 72-73 1.546639432563854 1101 74-75 1.285824290595727 1101 76-77 1.0009890799803927 1101 78-79 1.019483224509706 1101 80-81 1.0469388844331284 1101 82-83 0.6805342458274275 1101 84-85 0.5863144054787952 1101 86-87 -0.05667750105233793 1101 88-89 -0.37299071909976433 1101 90-91 -3.917736348595831 1101 92-93 -9.239059393706526 1101 94-95 -7.314946898107564 1101 96-97 -4.085537468771218 1101 98-99 -2.7454000161358394 1101 100-101 -2.059399369331583 1102 1 0.9684767647281198 1102 2 0.8823899578051417 1102 3 0.9161085505699873 1102 4 1.0725381372183094 1102 5 0.8651064377742799 1102 6 0.9449975321875854 1102 7 0.9425148049320455 1102 8 0.8778289165011586 1102 9 1.190104098323765 1102 10-11 0.9373846449316616 1102 12-13 0.9096531742750926 1102 14-15 1.2418339565287226 1102 16-17 1.181098771357945 1102 18-19 1.0784460362462411 1102 20-21 1.135887500851048 1102 22-23 1.1742092888927544 1102 24-25 1.1390108992994286 1102 26-27 0.9641810223811831 1102 28-29 1.1540120878623767 1102 30-31 1.1988180097062582 1102 32-33 1.1826830042858916 1102 34-35 1.0602682205627403 1102 36-37 1.0973491025711226 1102 38-39 0.9556704402613505 1102 40-41 1.168856066694957 1102 42-43 0.9975546163300919 1102 44-45 0.8018159021916347 1102 46-47 0.8705541790771321 1102 48-49 0.9412029244757463 1102 50-51 0.9296002350871042 1102 52-53 1.019233640550194 1102 54-55 0.9714136716129183 1102 56-57 1.069337466535604 1102 58-59 1.0270216627391413 1102 60-61 0.9534019242583547 1102 62-63 0.826427043890174 1102 64-65 0.6624455138870609 1102 66-67 0.7973759976285173 1102 68-69 0.7605239632196508 1102 70-71 0.8374405117814128 1102 72-73 0.7472917165172888 1102 74-75 0.5936812055493199 1102 76-77 0.33496134291871726 1102 78-79 0.22277079321063198 1102 80-81 0.21286226565819533 1102 82-83 0.27234339219668513 1102 84-85 0.13329972129314527 1102 86-87 -0.3894046909055966 1102 88-89 -1.0192628167480393 1102 90-91 -4.863063486151217 1102 92-93 -10.06553482930218 1102 94-95 -8.510015545056177 1102 96-97 -5.2497971540724 1102 98-99 -3.9104225785874096 1102 100-101 -3.2257929515675805 1103 1 0.8881104562779107 1103 2 0.8785985669422196 1103 3 0.9402321526826967 1103 4 0.8765954715955431 1103 5 0.8365352639685213 1103 6 0.7554423196103173 1103 7 0.8119048847708328 1103 8 0.7248827346344555 1103 9 0.8686183240407388 1103 10-11 0.7445588780591308 1103 12-13 0.8233375301184083 1103 14-15 0.8449752809376108 1103 16-17 0.9102191721807813 1103 18-19 0.9923933580300925 1103 20-21 1.0278782413927487 1103 22-23 0.9469989936846304 1103 24-25 0.7204186420421479 1103 26-27 0.7598788581945755 1103 28-29 0.9821781364834479 1103 30-31 0.9827521202978033 1103 32-33 0.9038633941442455 1103 34-35 0.7756311955633848 1103 36-37 0.8546500099668322 1103 38-39 0.8970522828890921 1103 40-41 0.8279011794852948 1103 42-43 0.6502936418609622 1103 44-45 0.7566179245852211 1103 46-47 1.0240067873282612 1103 48-49 0.6814673330269017 1103 50-51 0.5652944022748478 1103 52-53 0.7628634239945988 1103 54-55 0.8591862887935768 1103 56-57 0.7370631622488979 1103 58-59 0.6178789826584214 1103 60-61 0.5240699405461662 1103 62-63 0.6863567986256705 1103 64-65 0.6738270906031332 1103 66-67 0.5771034493142011 1103 68-69 0.7154133224696615 1103 70-71 0.7467982300108993 1103 72-73 0.6696426431716844 1103 74-75 0.6308517557205633 1103 76-77 0.5373067475272073 1103 78-79 0.2802298463963062 1103 80-81 0.14573692524746917 1103 82-83 0.36230839535498305 1103 84-85 -0.03838296296891386 1103 86-87 -0.27681816810510185 1103 88-89 -0.695446971565314 1103 90-91 -2.4427419272910313 1103 92-93 -8.690223597209222 1103 94-95 -5.280550732892516 1103 96-97 -2.85829731513207 1103 98-99 -2.106068521818294 1103 100-101 -1.721745043520155 1104 1 0.885309849419837 1104 2 0.9366095968033754 1104 3 0.8181738684228961 1104 4 0.95704371249807 1104 5 1.0688957549195806 1104 6 1.045806650161019 1104 7 1.07634832257866 1104 8 0.9139650415579581 1104 9 1.032574262118203 1104 10-11 0.8353341320551237 1104 12-13 1.0057868207991092 1104 14-15 1.0803952281610947 1104 16-17 1.2429431308511525 1104 18-19 1.2308493931760935 1104 20-21 1.206752174537705 1104 22-23 1.0665766377235428 1104 24-25 0.9654897177771673 1104 26-27 1.046213047169033 1104 28-29 1.0938464928615659 1104 30-31 0.8683604523728476 1104 32-33 1.0267431657815393 1104 34-35 1.0280183623846355 1104 36-37 0.8987173251950864 1104 38-39 0.8470080627808088 1104 40-41 0.765838437263362 1104 42-43 0.88365357916188 1104 44-45 0.8119110355956636 1104 46-47 0.861267801011337 1104 48-49 0.6580810074662367 1104 50-51 0.5861749216993353 1104 52-53 0.6709179549378881 1104 54-55 0.834333149884916 1104 56-57 1.0385310541361257 1104 58-59 0.7858183256123255 1104 60-61 0.8503082833346909 1104 62-63 0.5438512081973492 1104 64-65 0.6536422289797166 1104 66-67 0.5870296053284925 1104 68-69 0.5378983487500655 1104 70-71 0.9064346184863474 1104 72-73 0.9485043528720638 1104 74-75 0.7975148272564745 1104 76-77 0.824816544258514 1104 78-79 0.6466761315476965 1104 80-81 0.6771835339606298 1104 82-83 0.6380639120831226 1104 84-85 0.527886082236261 1104 86-87 0.36616916984437964 1104 88-89 0.349772583069889 1104 90-91 -0.2007572713090937 1104 92-93 -3.6025832972315115 1104 94-95 -1.1626340392623717 1104 96-97 -0.27614677981710045 1104 98-99 -0.22160883401944886 1104 100-101 -0.3355848588742383 1105 1 1.0394367894968823 1105 2 0.928362840587738 1105 3 0.9703994080612333 1105 4 0.9790678467003104 1105 5 0.7670651153919295 1105 6 0.7437200273225244 1105 7 0.8864451230796746 1105 8 0.8632790169176374 1105 9 1.124917782683525 1105 10-11 0.807516082759598 1105 12-13 0.8919526639597564 1105 14-15 1.061188239471143 1105 16-17 1.210773763999164 1105 18-19 1.0604081024035068 1105 20-21 0.9200694736931396 1105 22-23 0.8410405879367886 1105 24-25 1.0399258367830981 1105 26-27 0.8479410807174617 1105 28-29 1.0309034397116719 1105 30-31 1.1636510239997904 1105 32-33 1.194446259165737 1105 34-35 1.0191357680801687 1105 36-37 1.2280070260003058 1105 38-39 1.202219994633424 1105 40-41 1.082793756346689 1105 42-43 1.060024219603946 1105 44-45 1.1136197972956765 1105 46-47 1.232592061424242 1105 48-49 1.1538721686788307 1105 50-51 1.1197589751352517 1105 52-53 1.3020141057782446 1105 54-55 1.3364078640501766 1105 56-57 1.319674095377355 1105 58-59 1.2682575515060357 1105 60-61 1.1649964355870779 1105 62-63 1.2573019482894914 1105 64-65 1.3822967296607445 1105 66-67 1.2748921154428885 1105 68-69 1.2117003840699176 1105 70-71 1.1630608116131285 1105 72-73 1.391592723733666 1105 74-75 1.3191611003202262 1105 76-77 1.0018085356757638 1105 78-79 0.7858486488817285 1105 80-81 0.8656886605381295 1105 82-83 0.893375765147109 1105 84-85 0.8603552393042158 1105 86-87 0.668072776577457 1105 88-89 -0.0859332078414532 1105 90-91 -1.2204433980237575 1105 92-93 -6.959110765330621 1105 94-95 -2.780751198278246 1105 96-97 -1.1925631782728026 1105 98-99 -0.713749641321165 1105 100-101 -0.5501582838192931 1106 1 0.9099394821987161 1106 2 0.9612981205055533 1106 3 0.9758321133726078 1106 4 0.9916989738509443 1106 5 0.8218448674932048 1106 6 0.8476931752249328 1106 7 0.9074734037505081 1106 8 0.8571872631503297 1106 9 1.0746494648824907 1106 10-11 0.9692237484201272 1106 12-13 1.0956960972847511 1106 14-15 0.9092778878389467 1106 16-17 1.3066653143376357 1106 18-19 1.0103331181813004 1106 20-21 1.1952352199353626 1106 22-23 1.168195130355251 1106 24-25 1.0057413489297815 1106 26-27 1.0946401241448172 1106 28-29 1.0775398481388478 1106 30-31 1.0896164855432318 1106 32-33 0.9962072772749693 1106 34-35 0.8779606681752838 1106 36-37 1.114685929719549 1106 38-39 1.0467177336781717 1106 40-41 1.2298261652767337 1106 42-43 1.148720522710363 1106 44-45 1.0596484589193693 1106 46-47 1.0655989922266969 1106 48-49 1.086383670280739 1106 50-51 0.9862721389614926 1106 52-53 1.104318934101844 1106 54-55 1.236689007264328 1106 56-57 1.171644066132874 1106 58-59 1.2287879775764168 1106 60-61 1.3579200139027314 1106 62-63 1.2075949010424445 1106 64-65 1.1450376982207047 1106 66-67 0.960712668865817 1106 68-69 1.0174096611610608 1106 70-71 0.8792729909301791 1106 72-73 0.9081709987536968 1106 74-75 1.26774373344103 1106 76-77 1.022913800571338 1106 78-79 1.046527392600769 1106 80-81 1.0173477420099815 1106 82-83 0.9668774457841671 1106 84-85 0.7864205422442829 1106 86-87 0.6054795224907856 1106 88-89 0.46668098595434415 1106 90-91 -0.04161825030928412 1106 92-93 -3.163468008958869 1106 94-95 -1.2545256094203552 1106 96-97 -0.5476523593384286 1106 98-99 -0.12551270387743685 1106 100-101 0.2575882974462047 1107 1 0.6706987857822391 1107 2 0.551776485440783 1107 3 0.5783908095377797 1107 4 0.6275719002160258 1107 5 0.5027810640491239 1107 6 0.48536414571518804 1107 7 0.4180762557359614 1107 8 0.4234099607375157 1107 9 0.3880276854715774 1107 10-11 0.4279444309842759 1107 12-13 0.552102491420186 1107 14-15 0.5727817600543119 1107 16-17 0.6067463749599469 1107 18-19 0.5051196483269749 1107 20-21 0.5973159802196406 1107 22-23 0.47906460896228964 1107 24-25 0.5199979050332288 1107 26-27 0.4260171740285159 1107 28-29 0.5125742797491242 1107 30-31 0.5255597461722843 1107 32-33 0.33152105390315256 1107 34-35 0.3221126576082938 1107 36-37 0.3648741006915728 1107 38-39 0.30641436161757696 1107 40-41 0.37558680199336436 1107 42-43 0.10923679766064254 1107 44-45 0.08887226898314537 1107 46-47 0.0656719314634131 1107 48-49 0.1785578724695327 1107 50-51 0.3259737623192116 1107 52-53 0.4613330530540871 1107 54-55 0.4308995319671318 1107 56-57 0.3622494617975853 1107 58-59 0.4946229939264519 1107 60-61 0.3761301524566356 1107 62-63 0.2673943487882262 1107 64-65 0.4162668469462574 1107 66-67 0.31932532167811445 1107 68-69 0.3417874557604925 1107 70-71 0.3089272344307723 1107 72-73 0.42602885305297633 1107 74-75 0.3485193410892258 1107 76-77 -0.055029282306634286 1107 78-79 0.20052020287601025 1107 80-81 0.2665877029047401 1107 82-83 0.2454697798090919 1107 84-85 0.18517429263885887 1107 86-87 -0.036109173766327274 1107 88-89 -0.14405226790178105 1107 90-91 -0.34278313282501216 1107 92-93 -3.6289001739363886 1107 94-95 -1.4517457970396848 1107 96-97 -0.839680836287485 1107 98-99 -0.5322548330289187 1107 100-101 -0.5771635712386498 1108 1 0.33799718949951796 1108 2 0.26855158093520615 1108 3 0.010882519900103915 1108 4 -0.33582172027940516 1108 5 -1.02690372790574 1108 6 -0.287485960756662 1108 7 -0.0456017381656082 1108 8 0.14315758392623934 1108 9 0.33078790548486126 1108 10-11 0.17670505217810017 1108 12-13 0.12613852672896542 1108 14-15 0.2029120091898804 1108 16-17 0.2106451649358192 1108 18-19 0.24464241471146408 1108 20-21 0.3386130531916862 1108 22-23 0.13318832912908363 1108 24-25 0.1641896546574273 1108 26-27 0.11376814120387735 1108 28-29 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2311 98-99 1.3781748508350766 2311 100-101 1.4621367491272679 2312 1 0.19177753860697067 2312 2 -0.09248535720453432 2312 3 -0.0248441279760776 2312 4 -0.05793268304457655 2312 5 0.03171764379974462 2312 6 0.07461412330604134 2312 7 0.22086466147835893 2312 8 -0.4380989062087579 2312 9 -0.04489819450030552 2312 10-11 -0.03232674330228491 2312 12-13 0.0049337104306914625 2312 14-15 -0.2801827344188794 2312 16-17 0.050604617044804456 2312 18-19 0.07300615896306084 2312 20-21 0.28782311104659897 2312 22-23 0.08286814913600438 2312 24-25 0.17261446798878666 2312 26-27 0.1955685664622422 2312 28-29 0.07851426097261793 2312 30-31 0.1772482165473903 2312 32-33 0.19719931985964223 2312 34-35 0.08357234237053746 2312 36-37 0.15261782966825166 2312 38-39 0.1995351068279163 2312 40-41 0.16265377468251074 2312 42-43 0.31796917109612366 2312 44-45 0.11750125368359221 2312 46-47 0.23842511945964873 2312 48-49 -0.01665730253412434 2312 50-51 -0.07512947544893223 2312 52-53 -2.1072498683238905E-4 2312 54-55 -0.05046301503310957 2312 56-57 -0.060070639916364854 2312 58-59 0.035897225292654156 2312 60-61 0.0393909430513979 2312 62-63 -0.19500781758673114 2312 64-65 -0.0963661336592132 2312 66-67 0.10250218411425038 2312 68-69 0.04003494239756478 2312 70-71 0.19761273736283513 2312 72-73 0.1638942990893888 2312 74-75 0.4523833859641364 2312 76-77 0.6626124968382285 2312 78-79 0.6386946592772382 2312 80-81 0.5855760359943254 2312 82-83 0.7069451509753577 2312 84-85 0.5863429887094753 2312 86-87 0.6838236459650453 2312 88-89 0.9750497621311958 2312 90-91 1.4609942240277043 2312 92-93 2.2917302779735813 2312 94-95 1.67186424603306 2312 96-97 1.336218242342678 2312 98-99 1.058870318527692 2312 100-101 1.2042551165759647 2313 1 -0.2631398631707853 2313 2 -0.9414352809091682 2313 3 -0.4651797743524817 2313 4 -0.21206008721390646 2313 5 -0.7937385643448494 2313 6 -0.47108711299396333 2313 7 -1.33993369556444 2313 8 -1.0239496095504421 2313 9 -1.2150470714120658 2313 10-11 -2.004876716879558 2313 12-13 -0.4972842130130317 2313 14-15 -0.14364868946872633 2313 16-17 -1.5122819346929575 2313 18-19 -1.137936225277194 2313 20-21 -1.6313415689232755 2313 22-23 -1.3162378816812037 2313 24-25 -0.5266726839066607 2313 26-27 -0.8591141810229388 2313 28-29 -2.3649376485413853 2313 30-31 -0.6023354845078757 2313 32-33 -0.46190508699100974 2313 34-35 -0.21055911505957425 2313 36-37 -0.1282542292772959 2313 38-39 -0.47074238956585646 2313 40-41 -0.3800510547544178 2313 42-43 -0.1228023616576337 2313 44-45 -0.589822036792107 2313 46-47 -0.7221147673330179 2313 48-49 -0.32693924043204703 2313 50-51 -0.17758934106791813 2313 52-53 -0.19286107833267252 2313 54-55 -0.03802508405523852 2313 56-57 -0.3149178583855665 2313 58-59 -0.04760732336666251 2313 60-61 -0.27685036417955544 2313 62-63 -0.11890435228171015 2313 64-65 6.475094346463095E-4 2313 66-67 0.0055271989673286726 2313 68-69 -0.004662241085366503 2313 70-71 0.06262961374747533 2313 72-73 0.01169441232334023 2313 74-75 -0.0024125511022781154 2313 76-77 0.1372619241681683 2313 78-79 0.1576216899342704 2313 80-81 0.153187620466543 2313 82-83 0.19268707961755993 2313 84-85 0.3695585955604841 2313 86-87 0.3402746312379783 2313 88-89 0.6680646205377307 2313 90-91 1.3369936733876777 2313 92-93 2.468558216498902 2313 94-95 1.7227099288333427 2313 96-97 1.39687428045886 2313 98-99 1.20495714704969 2313 100-101 1.2672916966443921 2314 1 -0.4189646400092748 2314 2 -0.5984721862252833 2314 3 -1.7214696888441416 2314 4 -0.35574526414803387 2314 5 -0.18922178735451212 2314 6 -2.8452761690722177 2314 7 -3.923169958718862 2314 8 -1.4930639765363267 2314 9 -1.8045211341936636 2314 10-11 -1.0675817214383585 2314 12-13 -2.1235964919411146 2314 14-15 -2.9357030540969546 2314 16-17 -2.089163347901554 2314 18-19 -2.485432480665999 2314 20-21 -1.984310051584849 2314 22-23 -3.1956678949147204 2314 24-25 -1.9401287086180972 2314 26-27 -1.7602641046655023 2314 28-29 -1.263421056034339 2314 30-31 -1.528829715908529 2314 32-33 -2.271558957710184 2314 34-35 -0.737799038575595 2314 36-37 -0.4413062222629378 2314 38-39 -0.5225698730472743 2314 40-41 -0.7400105883113923 2314 42-43 -0.650440389457998 2314 44-45 -1.378627630698432 2314 46-47 -1.7692947038094928 2314 48-49 -1.9242859777751278 2314 50-51 -1.248910281386575 2314 52-53 -0.8667805357253542 2314 54-55 -0.6449944231987956 2314 56-57 -0.4802013998786556 2314 58-59 -0.6088915734887088 2314 60-61 -0.608028506439485 2314 62-63 -0.7024198378890141 2314 64-65 -0.6382018076774756 2314 66-67 -0.547461963138872 2314 68-69 -0.6260282699193702 2314 70-71 -0.7427981244141257 2314 72-73 -0.6486169048940127 2314 74-75 -0.43173197426232335 2314 76-77 -0.42989149200282384 2314 78-79 -0.2636845316814451 2314 80-81 -0.2566696480751922 2314 82-83 0.019950779499875892 2314 84-85 -0.3136208240963754 2314 86-87 -0.11048348186426793 2314 88-89 0.04728234478649185 2314 90-91 0.6872733287435864 2314 92-93 1.4611036263614992 2314 94-95 0.9903091019219588 2314 96-97 0.9501376699830075 2314 98-99 0.8202610349850836 2314 100-101 0.9195998023872818 2315 1 -7.055922400922579 2315 2 -2.966013347172879 2315 3 -1.4598284678491318 2315 4 -0.9429381500850909 2315 5 -1.3202360458020337 2315 6 -1.7052508260174264 2315 7 -3.153197241563376 2315 8 -3.0514903767434127 2315 9 -3.307262990552566 2315 10-11 -2.2967543051257167 2315 12-13 -2.9312403558168683 2315 14-15 -4.668361962928827 2315 16-17 -4.557105343554575 2315 18-19 -3.500015991923732 2315 20-21 -4.44008119082401 2315 22-23 -5.7825027804108515 2315 24-25 -3.810595774232649 2315 26-27 -4.94439616131266 2315 28-29 -3.1927304511708314 2315 30-31 -2.915348872490604 2315 32-33 -1.887565996564895 2315 34-35 -2.716351121267575 2315 36-37 -3.2809026599351085 2315 38-39 -2.557162679406609 2315 40-41 -3.8903045690710627 2315 42-43 -8.130170083401556 2315 44-45 -4.725791399928337 2315 46-47 -3.5266049966780173 2315 48-49 -6.275387420833027 2315 50-51 -7.235058163540046 2315 52-53 -7.1277610365353645 2315 54-55 -5.004487111912862 2315 56-57 -4.3768832670818405 2315 58-59 -4.578171560618568 2315 60-61 -3.528896340555022 2315 62-63 -3.8767984927871844 2315 64-65 -2.469600978665639 2315 66-67 -2.9359397777042417 2315 68-69 -2.3196460969179356 2315 70-71 -1.9508356067127153 2315 72-73 -2.5202955115291523 2315 74-75 -3.1038136665534104 2315 76-77 -3.0985924552110404 2315 78-79 -2.1393162046739462 2315 80-81 -1.811815618906845 2315 82-83 -2.1115722599718687 2315 84-85 -2.0267654814321894 2315 86-87 -2.5300313652094175 2315 88-89 -2.036379859039723 2315 90-91 -1.1954096122954105 2315 92-93 -0.36976921942919105 2315 94-95 -0.6559588220991905 2315 96-97 -0.7399822460962611 2315 98-99 -0.9055693836983387 2315 100-101 -0.8766833817543738 2316 1 -4.404235867395553 2316 2 -3.117873546022505 2316 3 -4.531858960538216 2316 4 -4.611950825956583 2316 5 -4.231542959378924 2316 6 -4.475645583700562 2316 7 -3.0750623279321765 2316 8 -1.5968269819993068 2316 9 -2.077713637922585 2316 10-11 -3.3944943366230618 2316 12-13 -4.110010873215078 2316 14-15 -3.340236779081174 2316 16-17 -2.9706902144199816 2316 18-19 -3.4671242889400844 2316 20-21 -5.970955717345589 2316 22-23 -6.945420482281229 2316 24-25 -4.72722001451757 2316 26-27 -4.3436419333822975 2316 28-29 -4.77137097413538 2316 30-31 -6.2660389517317725 2316 32-33 -4.657104363590435 2316 34-35 -6.402066271671927 2316 36-37 -9.47047368961774 2316 38-39 -6.407768924117455 2316 40-41 -5.718144138516827 2316 42-43 -4.851857035535545 2316 44-45 -3.5945108812177367 2316 46-47 -5.862362460327095 2316 48-49 -3.2751263571640408 2316 50-51 -4.778002712824794 2316 52-53 -5.535615008323521 2316 54-55 -7.135917593740793 2316 56-57 -5.188877770885508 2316 58-59 -6.042154560650243 2316 60-61 -6.629172621152929 2316 62-63 -6.744761370268844 2316 64-65 -5.5207593885927295 2316 66-67 -6.769320062900036 2316 68-69 -5.381144438329251 2316 70-71 -6.467087456502959 2316 72-73 -8.386114159677554 2316 74-75 -8.156374409818365 2316 76-77 -7.518847714951004 2316 78-79 -6.647087982418537 2316 80-81 -6.553285596867752 2316 82-83 -5.5119670515961054 2316 84-85 -6.890774446409495 2316 86-87 -5.4928580483488325 2316 88-89 -5.299420993396719 2316 90-91 -5.764613206884746 2316 92-93 -4.5738708888790285 2316 94-95 -4.422790499106581 2316 96-97 -5.131815620158921 2316 98-99 -6.0618678346262875 2316 100-101 -5.3288970772166735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15380.0 3 6367.0 4 6452.0 5 7476.0 6 8564.0 7 10199.0 8 12149.0 9 15255.0 10 18404.0 11 22156.0 12 25657.0 13 29129.0 14 31192.0 15 34766.0 16 38586.0 17 42280.0 18 39749.0 19 38450.0 20 40623.0 21 44248.0 22 48479.0 23 53207.0 24 59376.0 25 66561.0 26 74141.0 27 83723.0 28 94253.0 29 105701.0 30 119828.0 31 135444.0 32 154282.0 33 177919.0 34 206948.0 35 244191.0 36 275212.0 37 234915.0 38 71345.0 39 1174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.28552861453953 18.819020923729486 13.201448765955487 22.694001695775498 2 15.05203374929119 21.14451209859918 40.272962590547195 23.53049156156243 3 19.912047814479227 25.88057155915407 29.71598541190197 24.491395214464735 4 12.1752898688321 16.401245424321594 38.8283587829801 32.595105923866214 5 12.851988054617019 38.63399484774542 34.56380290341027 13.95021419422729 6 30.437916313256675 37.10375820180537 18.13809683563844 14.320228649299516 7 27.446152176950218 33.56883395608348 21.712949338755273 17.27206452821103 8 25.496952519948696 34.3751439768983 20.50743775367801 19.620465749474995 9 26.655285347263373 16.93703802091364 20.888485981100075 35.51919065072291 10-11 24.461879667483974 26.593237840065186 28.144610213650427 20.800272278800414 12-13 26.529794825998454 24.208380884510415 28.01368879413435 21.24813549535678 14-15 22.97048642333857 25.39875729961995 27.250137111721155 24.380619165320322 16-17 21.64664778106575 28.642042557998167 27.612258350264483 22.0990513106716 18-19 21.415570187050402 28.004294696117178 29.65235367591691 20.927781440915506 20-21 22.765842048768032 26.544507199744626 29.16890177068548 21.52074898080186 22-23 22.002713023441636 26.716721329753135 29.1791431677336 22.101422479071626 24-25 22.28857395102458 26.68355559685888 29.37811439988848 21.649756052228057 26-27 22.207096218730438 26.44814341731363 29.87829721679884 21.466463147157096 28-29 21.559252498280205 27.46923281241026 29.60679747085549 21.36471721845405 30-31 21.88313157229001 26.86145915177646 29.38311437859476 21.87229489733877 32-33 21.75162649385303 26.995996937621037 29.39409839648886 21.858278172037075 34-35 22.47429540030779 26.75222475819462 29.19122512248251 21.582254719015083 36-37 22.36385133119446 26.4844864205879 29.615064274911234 21.53659797330641 38-39 21.961953820768795 27.011118268626383 29.627801358754947 21.39912655184988 40-41 22.088333410769874 27.043163127816754 29.341169911257726 21.527333550155646 42-43 21.627450222423104 27.302194346647003 28.861181816019222 22.209173614910675 44-45 21.98239328720149 26.994506659520855 28.777198873940463 22.245901179337192 46-47 22.388715227632545 27.35240992631137 28.360735629825328 21.898139216230756 48-49 22.28679439737752 27.258521401704076 28.3877681603201 22.066916040598304 50-51 21.802349901780666 27.519206035274184 28.802564448653577 21.875879614291573 52-53 22.17982204320108 27.918125593114958 28.03420135118653 21.867851012497432 54-55 21.75223081067755 27.30338616181572 28.36763447613459 22.576748551372138 56-57 22.13275713854232 27.634283590065284 27.992936967077902 22.24002230431449 58-59 22.684207112415084 27.258929920551683 27.937791412251585 22.119071554781648 60-61 22.158849939580644 27.349190425232578 28.674903721170715 21.817055914016066 62-63 22.05095230086333 27.29939053116431 28.482590786139532 22.16706638183283 64-65 21.826169960567583 27.801348777773505 28.254205569965734 22.118275691693178 66-67 21.88109449796111 27.51084488442221 27.91999744279651 22.68806317482017 68-69 21.6808301232637 27.58545648899904 28.188041443721072 22.54567194401619 70-71 22.08977881376707 27.49081465024489 27.820035724779807 22.599370811208235 72-73 22.08955551292176 27.038930357692596 28.268345298062293 22.603168831323348 74-75 21.709550928430787 28.009530662293642 27.75155575139957 22.529362657876 76-77 21.924615847172245 27.287740931657883 27.860820846193278 22.926822374976595 78-79 21.849131046741448 27.335519893795635 28.06403870360764 22.751310355855278 80-81 21.971915211227223 27.48634279106325 27.83803951297981 22.703702484729714 82-83 21.924310662952493 27.351129806355836 27.996895475363598 22.727664055328074 84-85 21.772235153621114 27.350698732404815 27.800409016811106 23.076657097162965 86-87 21.69595498482664 27.406526367294248 27.928763651749605 22.96875499612951 88-89 21.784706773974502 27.183492829905774 27.63232563034429 23.399474765775434 90-91 21.53627386703078 26.964722416747914 28.091759717977343 23.407243998243963 92-93 21.49923477584586 27.33428819972481 28.33031112398199 22.83616590044734 94-95 21.72139423371923 27.165293131730312 27.716008682629944 23.397303951920513 96-97 21.206012002880215 27.052469457222262 28.03780133656267 23.70371720333486 98-99 21.59609236241066 27.12486907922355 27.397191734232727 23.881846824133056 100-101 21.510288373574234 26.86141825496654 27.73699681205326 23.89129655940597 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3174.0 1 1964.0 2 805.0 3 851.5 4 761.5 5 750.5 6 1090.0 7 1899.0 8 2954.0 9 3383.5 10 2904.5 11 2453.5 12 2486.0 13 2677.5 14 2899.5 15 3109.5 16 3390.0 17 3768.5 18 4049.5 19 4454.5 20 5110.0 21 5838.5 22 7002.5 23 8578.0 24 10050.5 25 12202.0 26 15555.0 27 19437.0 28 23419.0 29 27767.0 30 32537.0 31 37588.5 32 42940.5 33 48287.0 34 54277.0 35 60224.0 36 66177.5 37 72227.0 38 75804.5 39 77260.5 40 79141.0 41 82369.5 42 85504.5 43 88631.0 44 92347.0 45 95576.5 46 100200.5 47 111391.0 48 132276.0 49 132688.0 50 111785.5 51 98987.0 52 91792.0 53 86441.5 54 80717.0 55 74288.0 56 67631.0 57 60240.5 58 53432.0 59 46956.5 60 39572.5 61 32323.0 62 25795.5 63 20068.5 64 14996.5 65 10999.0 66 7875.0 67 5606.0 68 4022.5 69 2792.5 70 2047.5 71 1447.0 72 1016.0 73 722.0 74 509.0 75 360.5 76 239.0 77 146.0 78 91.0 79 58.0 80 34.5 81 26.5 82 22.5 83 15.0 84 11.5 85 9.0 86 7.5 87 8.5 88 6.5 89 4.0 90 3.0 91 2.5 92 4.5 93 5.0 94 3.0 95 1.0 96 1.5 97 1.5 98 2.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08861150925038078 2 0.09811488016286403 3 0.09451399352805592 4 0.09837473796125223 5 0.09399427793127949 6 0.07973922156255464 7 0.09254649876883086 8 0.0882402838241119 9 0.08300600531372075 10-11 0.08991079824232186 12-13 0.08844445780855979 14-15 0.09447687098542903 16-17 0.09239800859832333 18-19 0.09482953514038446 20-21 0.1045927638512559 22-23 0.1134464902677686 24-25 0.1357200158439012 26-27 0.1405088238427697 28-29 0.1403417724009487 30-31 0.14260624750118883 32-33 0.137928807130201 34-35 0.13367827599942236 36-37 0.13863413544011188 38-39 0.13081984021715204 40-41 0.1264393801871793 42-43 0.12669923798556748 44-45 0.11728867342965148 46-47 0.13653671178169272 48-49 0.15264789528176195 50-51 0.16304220721729049 52-53 0.17035534811478736 54-55 0.167496912332517 56-57 0.13804017475808167 58-59 0.13146948471312256 60-61 0.12688485069870192 62-63 0.12337677042046107 64-65 0.11042100304367727 66-67 0.12406353745905849 68-69 0.12209604269983343 70-71 0.12961335758177817 72-73 0.15624878191657005 74-75 0.16950152963436893 76-77 0.17501422721446175 78-79 0.17172888219198218 80-81 0.1788007265624043 82-83 0.17828101096562787 84-85 0.154448338599166 86-87 0.1558775564903012 88-89 0.17429033763323745 90-91 0.2615839966203637 92-93 0.5261749191935053 94-95 0.17573811679568604 96-97 0.18906510959873873 98-99 0.20823890286552618 100-101 0.21694413911153135 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2693781.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.83998782803285 #Duplication Level Percentage of deduplicated Percentage of total 1 77.06282417283649 44.57312812150697 2 12.635822683795947 14.617116604558777 3 4.458256907843742 7.73596575851776 4 2.042476846828543 4.725473438384073 5 1.1087932152784847 3.2066293037756473 6 0.692395941757862 2.402890370607244 7 0.4173682109394249 1.6898400568380922 8 0.29241658322797404 1.3530697291696776 9 0.21614403632888193 1.1251591587327978 >10 0.9953137393414317 9.919174611894313 >50 0.051408813906814135 2.0439681242296017 >100 0.02352206696434485 2.3927197320879943 >500 9.77034285056874E-4 0.3943006273255986 >1k 0.0018889329510777982 2.08283108937692 >5k 2.6054247601073083E-4 0.8799240829065271 >10k+ 1.3027123800536542E-4 0.857809190088145 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11895 0.4415726445468284 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10874 0.4036705285247762 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7165 0.2659830179216499 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5965 0.2214359667693847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5213 0.19351981471396523 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5013 0.18609530618858772 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4448 0.1651210696043962 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4240 0.15739958073800359 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3598 0.1335669083715417 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3361 0.12476886576896934 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2879 0.1068758002228095 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.424508525377528E-5 0.0 0.0 0.0 0.0 8 7.424508525377528E-5 0.0 0.0 3.712254262688764E-5 0.0 9 7.424508525377528E-5 0.0 0.0 7.424508525377528E-5 0.0 10-11 7.424508525377528E-5 0.0 0.0 1.1136762788066291E-4 0.0 12-13 1.113676278806629E-4 0.0 0.0 1.4849017050755055E-4 0.0 14-15 1.4849017050755055E-4 0.0 0.0 1.4849017050755055E-4 0.0 16-17 1.4849017050755055E-4 0.0 0.0 2.0417398444788198E-4 0.0 18-19 1.8561271313443818E-4 0.0 0.0 2.969803410151011E-4 0.0 20-21 1.8561271313443818E-4 0.0 0.0 3.7122542626887635E-4 0.0 22-23 1.8561271313443818E-4 0.0 0.0 6.496444959705336E-4 0.0 24-25 2.227352557613258E-4 0.0 0.0 8.909410230453032E-4 0.0 26-27 2.5985779838821345E-4 0.0 0.0 0.0013549728058813988 0.0 28-29 2.5985779838821345E-4 0.0 0.0 0.003953550789763533 0.0 30-31 2.5985779838821345E-4 0.0 0.0 0.010468557020782313 0.0 32-33 2.969803410151011E-4 0.0 0.0 0.02444519431980551 0.0 34-35 4.2690924020920783E-4 0.0 0.0 0.04467698005145927 0.0 36-37 5.568381394033146E-4 0.0 0.0 0.07704783722210529 0.0 38-39 7.795733951646404E-4 0.0 0.0 0.1257711744198953 0.0 40-41 7.795733951646404E-4 0.0 0.0 0.18006289301171846 0.0 42-43 7.981346664780841E-4 0.0 0.0 0.23546828788234825 0.0 44-45 8.538184804184156E-4 0.0 0.0 0.301787710285283 0.0 46-47 9.837473796125223E-4 0.0 0.0 0.38009771395670244 0.0 48-49 0.001002308650925966 0.0 0.0 0.46668604463391794 0.0 50-51 0.001002308650925966 0.0 0.0 0.5620538566423923 0.0 52-53 0.001002308650925966 0.0 0.0 0.656772024154896 0.0 54-55 0.001002308650925966 0.0 0.0 0.7603067955412857 0.0 56-57 0.0010765537361797414 0.0 0.0 0.8866904919145246 0.0 58-59 0.0010765537361797414 0.0 0.0 1.0236169903937997 0.0 60-61 0.0010765537361797414 0.0 0.0 1.179049076372578 0.0 62-63 0.0010765537361797414 0.0 0.0 1.3396968795904345 0.0 64-65 0.0010765537361797414 0.0 0.0 1.501328430187903 0.0 66-67 0.0011136762788066291 0.0 0.0 1.682746296005503 0.0 68-69 0.0011507988214335167 0.0 0.0 1.8913378630259845 0.0 70-71 0.0011693600927469606 0.0 0.0 2.121330575870867 0.0 72-73 0.001225043906687292 0.0 0.0 2.358933409954261 0.0 74-75 0.0012807277206276233 0.0 0.0 2.6048516935860784 0.0 76-77 0.0013364115345679548 0.0 0.0 2.863373080439724 0.0 78-79 0.0013364115345679548 0.0 0.0 3.1661630993759333 0.0 80-81 0.001522024247702393 0.0 1.856127131344382E-5 3.506316957466104 0.0 82-83 0.001522024247702393 0.0 3.712254262688764E-5 3.8363549226904485 0.0 84-85 0.001540585519015837 0.0 3.712254262688764E-5 4.171014644471841 0.0 86-87 0.0015591467903292807 3.712254262688764E-5 3.712254262688764E-5 4.535483767982623 0.0 88-89 0.0015591467903292807 3.712254262688764E-5 3.712254262688764E-5 4.923265848263092 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11665 0.0 26.8514 1 TACATGG 11580 0.0 26.393265 2 ACATGGG 11880 0.0 24.688112 3 GAGTACT 9160 0.0 22.069967 12-13 CATGGGA 8345 0.0 21.725056 4 AGTACTT 9460 0.0 20.96876 12-13 CATGGGG 4080 0.0 20.821728 4 GTACTTT 10080 0.0 19.939428 14-15 GTGTAGC 1560 0.0 18.557224 1 ATGGGAG 3365 0.0 18.475748 5 GTATAGG 690 0.0 17.882656 1 TAGCCCT 1805 0.0 17.879509 4 ATGGGAT 3725 0.0 17.836824 5 ACTTTTT 11935 0.0 17.358892 16-17 AGTACAT 8305 0.0 17.314854 2 GTCCTAG 1525 0.0 17.115934 1 TACTTTT 11670 0.0 16.877071 14-15 AGAGTAC 19625 0.0 16.237528 10-11 GTATCAA 32205 0.0 16.01821 1 AGCACCG 560 1.8189894E-12 15.254581 5 >>END_MODULE