FastQCFastQC Report
Fri 27 May 2016
SRR939635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939635_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693781
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT135320.5023422468270435No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115690.42947069565046303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84730.3145393036776189No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT70050.2600434111013479No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64310.23873507163351437No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55730.20688393005964478No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC51490.19114397198584443No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA45010.16708856436362124No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA39760.14759922948450524No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37250.13828147128515644No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35840.1330471927747653No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31750.11786407284036823No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30090.1117017307643049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG112450.027.3348621
TACATGG113700.026.2827912
ACATGGG113100.025.1739273
CATGGGG40100.022.298084
CATGGGA79500.021.7840654
GAGTACT95200.020.34051312-13
AGTACTT99950.019.63248812-13
ATGGGAT36550.018.9272795
GTACTTT102350.018.73569914-15
ATGGGAG33000.018.5390575
TTACGCG1659.1768015E-917.66673362-63
GCTTACG6750.017.43249560-61
TAGCCCT18700.017.3646374
AGTACAT84750.016.7697052
ATGGGGA24500.016.7113445
GTATAGG9900.016.6416381
TACTTTT116150.016.59060714-15
ACTTTTT117550.016.5331116-17
AGAGTAC193350.016.50544410-11
GTATCAA281550.016.0501421
Produced by FastQC (version 0.11.5)