FastQCFastQC Report
Fri 27 May 2016
SRR939634_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939634_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720912
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50810.18673885814756228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45240.16626778080290727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30380.11165373962847751No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28430.10448702493869703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG100950.031.0271431
TACATGG116800.025.6820562
ACATGGG116600.024.7947523
GAGTACT95450.022.12687112-13
AGTACTT100500.021.29679112-13
CATGGGA84900.021.085044
GTACTTT102550.020.80547514-15
GTATAAT13150.019.9129371
GTGTAGC14250.019.7893221
GTATAGG8150.019.7719761
CATGGGG39900.019.5323244
GTATAGA9350.018.3115481
AGTACAT85650.017.704212
GTATCAA297650.017.459491
TACTTTT123850.017.34164414-15
ACTTTTT129900.017.26550716-17
AGAGTAC201900.016.47979410-11
GTATAGC9258.367351E-1116.331921
ATGGGAT37400.015.8988575
GAGTACA130650.015.8798191
Produced by FastQC (version 0.11.5)