##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939634_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2720912 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.138908204307967 2.0 2.0 31.0 2.0 33.0 2 22.413389701688256 16.0 16.0 31.0 16.0 34.0 3 26.772677322897618 28.0 25.0 31.0 16.0 34.0 4 31.980668614052934 35.0 32.0 35.0 25.0 37.0 5 32.61967310960443 35.0 33.0 35.0 26.0 37.0 6 33.000559003745806 35.0 33.0 37.0 26.0 37.0 7 33.16692087064925 35.0 33.0 37.0 26.0 37.0 8 33.08872062014501 35.0 33.0 37.0 26.0 37.0 9 34.39674123970198 37.0 33.0 39.0 27.0 39.0 10-11 34.23214238461222 37.0 33.0 39.0 26.0 39.0 12-13 34.23808855266175 37.0 33.0 39.0 26.5 39.0 14-15 34.86823719399966 38.0 33.0 40.0 25.5 41.0 16-17 34.48803765061126 38.0 33.0 40.0 25.0 41.0 18-19 34.784147925401484 38.0 33.0 40.0 25.0 41.0 20-21 34.782002321280515 38.0 33.0 40.0 25.0 41.0 22-23 34.59657717706416 38.0 33.0 40.0 24.5 41.0 24-25 34.38670526646948 38.0 33.0 40.0 24.0 41.0 26-27 34.45406282893383 38.0 33.0 40.0 24.0 41.0 28-29 34.19155819813356 38.0 32.5 40.0 23.0 41.0 30-31 34.0681565592713 38.0 32.5 40.0 23.0 41.0 32-33 33.7428707727409 38.0 32.0 40.0 19.5 41.0 34-35 33.44907626560506 37.0 31.0 40.0 18.5 41.0 36-37 33.14601372628001 37.0 31.0 40.0 16.5 41.0 38-39 32.93042773893459 37.0 31.0 40.0 16.0 41.0 40-41 32.55705054040704 37.0 30.0 40.0 15.0 41.0 42-43 32.29136388828452 37.0 30.0 40.0 15.0 41.0 44-45 31.9241528208189 36.5 30.0 39.0 9.5 41.0 46-47 31.570751828798578 36.0 30.0 39.0 9.0 40.0 48-49 31.177989034559 36.0 29.0 39.0 8.0 40.0 50-51 30.52853252879917 35.5 28.0 38.5 7.0 40.0 52-53 30.5798936165521 35.5 28.0 38.5 4.5 40.0 54-55 30.699982211846617 36.0 28.0 39.0 2.0 40.0 56-57 30.39868433819249 35.0 28.0 39.0 2.0 40.0 58-59 29.97069364977625 35.0 27.5 38.5 2.0 40.0 60-61 29.349227942689804 34.0 26.0 38.0 2.0 40.0 62-63 28.95743026602845 34.0 26.0 38.0 2.0 40.0 64-65 28.372716574442688 34.0 25.5 38.0 2.0 39.5 66-67 27.752754407345773 33.0 23.5 37.0 2.0 39.0 68-69 27.38965152125464 33.0 23.0 36.5 2.0 39.0 70-71 26.836896231851675 32.0 22.0 36.0 2.0 39.0 72-73 26.222599812121818 32.0 20.5 35.5 2.0 38.0 74-75 25.650428055005086 31.0 19.5 35.0 2.0 37.5 76-77 24.99217817408281 31.0 17.5 35.0 2.0 37.0 78-79 24.302075186555093 30.5 10.5 34.0 2.0 36.0 80-81 23.531270948858324 30.0 7.0 34.0 2.0 36.0 82-83 22.812154527599567 29.0 3.5 34.0 2.0 35.0 84-85 22.093403976313823 29.0 2.0 34.0 2.0 35.0 86-87 21.399586241671912 29.0 2.0 33.5 2.0 35.0 88-89 20.5458147856307 27.0 2.0 33.0 2.0 34.5 90-91 19.54920188525024 26.0 2.0 32.0 2.0 34.0 92-93 18.480820585156742 24.5 2.0 32.0 2.0 34.0 94-95 17.467177916816127 21.5 2.0 31.5 2.0 34.0 96-97 16.529738742010032 18.5 2.0 31.0 2.0 34.0 98-99 15.477550541877136 2.0 2.0 31.0 2.0 34.0 100-101 13.951729052611771 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.860024006029423 1101 2 8.215043561156996 1101 3 4.11203710281411 1101 4 2.675623612060008 1101 5 1.8844612677045731 1101 6 1.7703958640400899 1101 7 1.3093928620267334 1101 8 1.256333814695303 1101 9 1.2700544540407464 1101 10-11 1.2988650577451395 1101 12-13 1.0089926470342405 1101 14-15 1.141806482829189 1101 16-17 1.313266656235136 1101 18-19 1.3407737803056037 1101 20-21 1.1614142269322016 1101 22-23 1.143238322546523 1101 24-25 1.4004639796362213 1101 26-27 1.5741548200224926 1101 28-29 1.57526070105731 1101 30-31 1.5611426419079564 1101 32-33 1.4775629952567826 1101 34-35 1.4459227944402286 1101 36-37 1.6529790350681495 1101 38-39 1.5024958394189198 1101 40-41 1.5358069456370629 1101 42-43 1.5198897484799403 1101 44-45 1.2984633203056504 1101 46-47 1.063963786693968 1101 48-49 0.9654679281879375 1101 50-51 0.9565697841968372 1101 52-53 1.2153806140637045 1101 54-55 1.1281123113667384 1101 56-57 1.0962830638896541 1101 58-59 1.1683477739188213 1101 60-61 1.0482393605604834 1101 62-63 0.9352466849412799 1101 64-65 0.9685894578565666 1101 66-67 0.8974549709752857 1101 68-69 0.6970740872393755 1101 70-71 0.4869022731722623 1101 72-73 0.578646605023927 1101 74-75 0.3871396561960836 1101 76-77 0.4105295443292647 1101 78-79 0.37127639822965364 1101 80-81 0.28030817988695134 1101 82-83 0.3307911964816981 1101 84-85 0.2925271815621926 1101 86-87 0.1544207435025342 1101 88-89 0.24204612916616242 1101 90-91 0.38281533991765215 1101 92-93 0.4028573775895481 1101 94-95 0.6100106001662269 1101 96-97 -0.024349564386042744 1101 98-99 -0.40191116819132056 1101 100-101 -0.45500171016241 1102 1 14.812248498966454 1102 2 7.998004791789413 1102 3 3.865694711323872 1102 4 2.413383129110322 1102 5 1.8345288190949347 1102 6 1.4408562395916178 1102 7 1.1840422788615683 1102 8 1.1344420337328245 1102 9 1.2789515707079815 1102 10-11 1.1771058143207043 1102 12-13 0.8639762722351847 1102 14-15 1.0832868843751697 1102 16-17 1.2442648349128476 1102 18-19 1.1398575611312722 1102 20-21 1.1142191060237607 1102 22-23 0.9590645872111594 1102 24-25 0.8789736591519031 1102 26-27 1.1049516271504487 1102 28-29 1.1163975738554797 1102 30-31 1.0930585361515526 1102 32-33 1.1157643753573367 1102 34-35 1.1462222865221818 1102 36-37 1.0398828299171399 1102 38-39 0.965390673020174 1102 40-41 0.9442170937370733 1102 42-43 1.0257962197321149 1102 44-45 0.8847324313635525 1102 46-47 0.5139030759507293 1102 48-49 0.30532089702878906 1102 50-51 0.3471514957267452 1102 52-53 0.4986400541567342 1102 54-55 0.6238785921068946 1102 56-57 0.5963407818497721 1102 58-59 0.5580829553669879 1102 60-61 0.6331510307044788 1102 62-63 0.09042569612573814 1102 64-65 0.016339312492817726 1102 66-67 0.19925042707543383 1102 68-69 -0.2523799436119418 1102 70-71 0.05637011357996258 1102 72-73 0.21398773954525296 1102 74-75 0.13795198304220335 1102 76-77 -0.1187441499622146 1102 78-79 -0.06934892242568225 1102 80-81 -0.013595948443594352 1102 82-83 -0.2077173714288243 1102 84-85 -0.24523105286678515 1102 86-87 -0.1876565045051244 1102 88-89 -0.26577465442996484 1102 90-91 0.14490404600924833 1102 92-93 0.23828530282127502 1102 94-95 0.041248201493313985 1102 96-97 -0.7153008846309383 1102 98-99 -0.6892739277083724 1102 100-101 -0.7026203098819437 1103 1 14.793341861704942 1103 2 7.939124749502266 1103 3 3.9461525255500653 1103 4 2.3363399399477487 1103 5 1.8864120349869609 1103 6 1.2861499842384845 1103 7 1.0191317149363925 1103 8 1.1284387690988638 1103 9 1.1822124807505574 1103 10-11 1.0335830767037706 1103 12-13 0.6790698253072165 1103 14-15 0.8537539463287374 1103 16-17 1.070152316758751 1103 18-19 0.9306860447522425 1103 20-21 0.8951614949579394 1103 22-23 1.0161615455984574 1103 24-25 1.1580714161125272 1103 26-27 1.272073913345892 1103 28-29 1.1793957770655012 1103 30-31 1.0897692053808257 1103 32-33 1.172454547526435 1103 34-35 1.1124940714370055 1103 36-37 1.2310283895598104 1103 38-39 1.120714348650452 1103 40-41 0.9411014164600005 1103 42-43 1.0773422142455473 1103 44-45 0.9368589948915158 1103 46-47 0.973775412832822 1103 48-49 0.8412014844560112 1103 50-51 1.0246605365698436 1103 52-53 1.1317448751811447 1103 54-55 1.048020461968406 1103 56-57 1.036290550346262 1103 58-59 0.8318053414875344 1103 60-61 1.0172980312961677 1103 62-63 1.0122151624056706 1103 64-65 0.5746954203374557 1103 66-67 0.7998590289100846 1103 68-69 0.6270692321610802 1103 70-71 0.5698077648640947 1103 72-73 0.6854893004871556 1103 74-75 0.5988789125484537 1103 76-77 0.6189635618562583 1103 78-79 0.33569468360871824 1103 80-81 0.2699657247950995 1103 82-83 0.009023264988599067 1103 84-85 -0.12103945319614695 1103 86-87 -0.08028020061694363 1103 88-89 -0.3840461310872101 1103 90-91 -0.288637223267191 1103 92-93 0.16024376769853177 1103 94-95 -0.01083478703288776 1103 96-97 -0.658380036488138 1103 98-99 -0.8537936096444785 1103 100-101 -0.6465659189699551 1104 1 14.89724273700886 1104 2 8.135806119695882 1104 3 4.098839708330516 1104 4 2.618949394687526 1104 5 1.8065040212231835 1104 6 1.5764408995033037 1104 7 1.0615286555097043 1104 8 1.1416590388634162 1104 9 1.2296051033805284 1104 10-11 1.088318606786025 1104 12-13 0.9562235276431537 1104 14-15 1.1094143993572914 1104 16-17 1.188751974713874 1104 18-19 0.9816141179120947 1104 20-21 1.2509776115517681 1104 22-23 1.2036775655098353 1104 24-25 1.3194357293292782 1104 26-27 1.3212120579282995 1104 28-29 1.3032335823104404 1104 30-31 1.248636563171452 1104 32-33 1.3764902747437517 1104 34-35 1.2583516797594356 1104 36-37 1.3184428308979008 1104 38-39 1.1734546169575637 1104 40-41 1.3159573479579123 1104 42-43 1.2273649995252214 1104 44-45 0.8748436248856422 1104 46-47 0.9432662902444733 1104 48-49 0.8838723291408748 1104 50-51 1.0064232670383255 1104 52-53 0.9240633767017243 1104 54-55 1.120631935881221 1104 56-57 0.8782343409210753 1104 58-59 0.8554576889786141 1104 60-61 0.7907523834364021 1104 62-63 0.5759569506591937 1104 64-65 0.43171937182872 1104 66-67 0.5416740402723583 1104 68-69 0.6547772582220759 1104 70-71 0.49885091216276933 1104 72-73 0.33913249085139796 1104 74-75 0.2921804451716348 1104 76-77 0.16552966459673968 1104 78-79 0.18038871184121064 1104 80-81 0.06349775437016447 1104 82-83 0.026973789176551577 1104 84-85 -0.004095681598521139 1104 86-87 -0.14690993767152705 1104 88-89 -0.18640413552686041 1104 90-91 -0.15993556418001376 1104 92-93 0.11601552827318784 1104 94-95 0.35846803907557856 1104 96-97 -0.14888372400921668 1104 98-99 -0.3307039990994358 1104 100-101 -0.41916768921286085 1105 1 14.696279784181577 1105 2 7.9015959234564725 1105 3 3.948809491338306 1105 4 2.466655753939058 1105 5 1.7929491346224253 1105 6 1.3749249187053465 1105 7 1.1079573491093981 1105 8 1.196383152023202 1105 9 1.29664121553688 1105 10-11 1.0603023238181564 1105 12-13 0.9007682202850305 1105 14-15 1.0114956659623786 1105 16-17 1.2358562746488744 1105 18-19 1.1938585562751243 1105 20-21 1.2140957770952738 1105 22-23 1.1748973057577246 1105 24-25 1.175272169881623 1105 26-27 1.2335514232574312 1105 28-29 1.4624357967028558 1105 30-31 1.3321081534974581 1105 32-33 0.9717892519270706 1105 34-35 1.0992663424123776 1105 36-37 1.1306841335353326 1105 38-39 1.3460121717757474 1105 40-41 1.3519169940200086 1105 42-43 1.4533995039095373 1105 44-45 1.2169270579920664 1105 46-47 1.130342254622633 1105 48-49 1.1355214357441419 1105 50-51 1.102406402535884 1105 52-53 1.1513421940200494 1105 54-55 1.2059721336029732 1105 56-57 1.1797970725371698 1105 58-59 1.2265551359278355 1105 60-61 1.1332961980751115 1105 62-63 0.954209553311447 1105 64-65 1.0490012108462672 1105 66-67 1.1026439487091722 1105 68-69 0.939310149133032 1105 70-71 0.9118259745922153 1105 72-73 1.120888839270421 1105 74-75 1.0777338722346563 1105 76-77 0.9267441596308927 1105 78-79 0.9601595758191337 1105 80-81 0.7347154991563087 1105 82-83 0.8790929695548897 1105 84-85 0.841351529886218 1105 86-87 0.7404747531459286 1105 88-89 0.7062713795873385 1105 90-91 0.9305686034843816 1105 92-93 0.927679572332984 1105 94-95 0.7846715875766996 1105 96-97 0.4116828160881596 1105 98-99 0.12354148830634948 1105 100-101 0.10188585757786583 1106 1 14.651659363618336 1106 2 7.815195073206297 1106 3 3.738059553214388 1106 4 2.2383291258701306 1106 5 1.6512885746802723 1106 6 1.2066479370934644 1106 7 0.940726679925632 1106 8 0.9724468140196407 1106 9 1.0858019817326436 1106 10-11 0.9378815858670038 1106 12-13 0.7390402760754213 1106 14-15 0.875615424087556 1106 16-17 1.1606690691669002 1106 18-19 0.9610197058599468 1106 20-21 0.9620667268810124 1106 22-23 0.8165110339979691 1106 24-25 0.9612000927379185 1106 26-27 0.8492774116975212 1106 28-29 0.95328966238079 1106 30-31 0.8576815385317005 1106 32-33 0.8019882987927431 1106 34-35 0.7716299359811458 1106 36-37 0.975727980626182 1106 38-39 1.1960916400782011 1106 40-41 1.2152573561528186 1106 42-43 0.7946461007249752 1106 44-45 0.9190054877469365 1106 46-47 0.8783795695403427 1106 48-49 0.8452669187900916 1106 50-51 0.7120856417755128 1106 52-53 0.4730501437621122 1106 54-55 0.5772109170463899 1106 56-57 0.8480548846959444 1106 58-59 0.8105211016557661 1106 60-61 1.0327041288903693 1106 62-63 0.9069086851050763 1106 64-65 1.0072460168950812 1106 66-67 0.9044174870990354 1106 68-69 0.9303866473835178 1106 70-71 0.9079467081756398 1106 72-73 0.6806206125176288 1106 74-75 0.6814207161661905 1106 76-77 0.6464535118974979 1106 78-79 0.5972654494366267 1106 80-81 0.615883695335036 1106 82-83 0.6680205478463321 1106 84-85 0.4396630407788322 1106 86-87 0.39125147997394194 1106 88-89 0.3662593926128572 1106 90-91 0.5714498887598154 1106 92-93 1.092332920678345 1106 94-95 0.9043381118879346 1106 96-97 0.5380860685862565 1106 98-99 0.057711766433277134 1106 100-101 0.12965475162174833 1107 1 14.687031661189028 1107 2 7.963696942959739 1107 3 3.74603390542509 1107 4 2.2126788300396854 1107 5 1.4103239657171542 1107 6 1.1539316323421573 1107 7 0.988370360899026 1107 8 0.7331991794099224 1107 9 0.7600742221305339 1107 10-11 0.8954459410736106 1107 12-13 0.7624445999623077 1107 14-15 0.7520948448638478 1107 16-17 1.1358837058809073 1107 18-19 0.9990636083630235 1107 20-21 0.8502533823438796 1107 22-23 0.9031737777432767 1107 24-25 0.9711100246915478 1107 26-27 0.8528036246500861 1107 28-29 0.9374197346763324 1107 30-31 0.9217137605960986 1107 32-33 0.9945142980932999 1107 34-35 0.990518742645186 1107 36-37 0.8903544894688693 1107 38-39 0.8827380316868059 1107 40-41 1.0450690917351864 1107 42-43 0.7890508650453327 1107 44-45 0.7948015801830621 1107 46-47 0.6869761981355822 1107 48-49 0.7356987106858774 1107 50-51 0.6766854739572317 1107 52-53 0.8333340860832301 1107 54-55 0.7704259739028885 1107 56-57 0.802443344623061 1107 58-59 0.8041190611000779 1107 60-61 0.9223711898384828 1107 62-63 0.7997200112872314 1107 64-65 0.6378421179250253 1107 66-67 0.7226889707630093 1107 68-69 0.8247781032130561 1107 70-71 0.6495901695290947 1107 72-73 0.6515016346097227 1107 74-75 0.6088996463783758 1107 76-77 0.4520806760192393 1107 78-79 0.6957906538085012 1107 80-81 0.5992883653620424 1107 82-83 0.5032785594981242 1107 84-85 0.7539180457859302 1107 86-87 0.8681549223971139 1107 88-89 0.8734176896512125 1107 90-91 1.025364494798648 1107 92-93 1.2779170798741575 1107 94-95 1.1982970119262468 1107 96-97 0.8185413860743296 1107 98-99 0.566165522710051 1107 100-101 0.64057750150209 1108 1 14.173392667616575 1108 2 7.492245696243678 1108 3 3.363357071065458 1108 4 -0.26491506878155135 1108 5 0.19594925432105015 1108 6 0.5361257057595878 1108 7 0.48963561446170445 1108 8 0.5073169062251708 1108 9 0.49486452548526927 1108 10-11 0.5333303127906177 1108 12-13 0.3091904850879601 1108 14-15 0.36609885309329826 1108 16-17 0.6197536142396061 1108 18-19 0.3740784893869673 1108 20-21 0.39598976226313454 1108 22-23 -0.24316254641685475 1108 24-25 0.3030216020284726 1108 26-27 0.5337704812816995 1108 28-29 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6 -0.7248971735120051 2312 7 -0.3798133099316061 2312 8 0.002267206356023621 2312 9 -0.14763390120373288 2312 10-11 -0.06044530726150299 2312 12-13 -0.777668465100156 2312 14-15 -0.11028725925632443 2312 16-17 -0.28573664578559743 2312 18-19 -0.054125883160729416 2312 20-21 0.09344387604692628 2312 22-23 0.07098383479143422 2312 24-25 0.28462224255458324 2312 26-27 0.3747552659200082 2312 28-29 -0.38556833446866534 2312 30-31 0.13573334291103123 2312 32-33 0.26762733765388447 2312 34-35 -0.5785329765173373 2312 36-37 0.06244567525725131 2312 38-39 0.3699837902090266 2312 40-41 0.3965910844255447 2312 42-43 0.264299720927923 2312 44-45 0.2910323346495787 2312 46-47 0.3115325995781788 2312 48-49 0.22884821122924848 2312 50-51 0.18440633704835463 2312 52-53 0.13724350249723116 2312 54-55 -0.031943598502106596 2312 56-57 -0.0016786434143192253 2312 58-59 0.3146626919151245 2312 60-61 0.1869263900081357 2312 62-63 0.10376510219697721 2312 64-65 0.17962427009610593 2312 66-67 0.0343638785647542 2312 68-69 0.4795515525307472 2312 70-71 0.32667536237478956 2312 72-73 0.0842562988818969 2312 74-75 0.3970214952738971 2312 76-77 0.3167428498803453 2312 78-79 0.415094606734705 2312 80-81 0.3992886114328549 2312 82-83 0.22795776145917657 2312 84-85 0.24364777726898978 2312 86-87 0.2228863495317448 2312 88-89 0.6955348359098856 2312 90-91 0.9814260353182327 2312 92-93 1.603218662019973 2312 94-95 1.4716298374589165 2312 96-97 1.0780325049314499 2312 98-99 1.0720489638895092 2312 100-101 1.2335969648082354 2313 1 -14.065641674443949 2313 2 -7.338884641397133 2313 3 -3.748342903363678 2313 4 -1.236495464747236 2313 5 -0.570947069409435 2313 6 -1.1727720124428238 2313 7 -0.5403742644344547 2313 8 -0.265628452551816 2313 9 -2.42146050295608 2313 10-11 -0.6265615352063989 2313 12-13 -1.036214441491488 2313 14-15 -1.1860513460654687 2313 16-17 -0.9423483108971098 2313 18-19 -0.28202459947373626 2313 20-21 -1.3034110696497478 2313 22-23 -2.2874521173310853 2313 24-25 -1.5968910354703922 2313 26-27 -0.6812124927375223 2313 28-29 0.06022418938226082 2313 30-31 -1.5836580641935711 2313 32-33 -0.5947761530776319 2313 34-35 -1.4780251721997288 2313 36-37 -1.440208448642771 2313 38-39 -0.827131828370689 2313 40-41 -0.37646862701465267 2313 42-43 -0.15982563903128266 2313 44-45 -0.39815048125230845 2313 46-47 -0.7473487392479043 2313 48-49 -0.23666859603573798 2313 50-51 -0.9287840387903152 2313 52-53 -0.23784160762225426 2313 54-55 -0.9665147048596019 2313 56-57 -0.6247881302568956 2313 58-59 -0.40569840985097727 2313 60-61 -1.9334245027753276 2313 62-63 -0.6635460527827846 2313 64-65 -0.3993094677880009 2313 66-67 -1.647890635075079 2313 68-69 -0.6122399493639996 2313 70-71 0.0014674270859877936 2313 72-73 -1.0281444941391769 2313 74-75 -0.6077129336472531 2313 76-77 0.09388149950171254 2313 78-79 0.12774575786361808 2313 80-81 0.13284125275585268 2313 82-83 0.03982662945755777 2313 84-85 0.2719784446296529 2313 86-87 0.1033246692918901 2313 88-89 0.5035652851262782 2313 90-91 0.9029245129632066 2313 92-93 1.2689319260803344 2313 94-95 1.1823587376605538 2313 96-97 0.8631900723795027 2313 98-99 0.7456690585526982 2313 100-101 0.6563135909563496 2314 1 -14.065641674443949 2314 2 -7.088435129479262 2314 3 -3.440326039760059 2314 4 -1.2948431255632826 2314 5 -1.1947056746162517 2314 6 -6.003937385299803 2314 7 -1.733577324516542 2314 8 -4.303610506407416 2314 9 -1.4816648982433236 2314 10-11 -0.3501143611045521 2314 12-13 -3.1116404438207184 2314 14-15 -2.9270083485969423 2314 16-17 -2.4608285373616425 2314 18-19 -1.1471252655742745 2314 20-21 -1.8387546315093388 2314 22-23 -1.1765639993695416 2314 24-25 -2.863745546387804 2314 26-27 -4.596424068306433 2314 28-29 -1.9832642848843207 2314 30-31 -1.9658033832239212 2314 32-33 -4.053137439530907 2314 34-35 -2.557981806195265 2314 36-37 -0.8506507829611891 2314 38-39 -1.3424091717791242 2314 40-41 -2.488668346669847 2314 42-43 -1.3470400706195846 2314 44-45 -2.302545768498444 2314 46-47 -1.9675093715633025 2314 48-49 -1.0271753892742055 2314 50-51 -1.9980202467218326 2314 52-53 -0.7848534892852008 2314 54-55 -0.3496333986437925 2314 56-57 -1.0809672226285265 2314 58-59 -1.506059221016276 2314 60-61 -0.6509757182733615 2314 62-63 -2.7547608963588637 2314 64-65 -1.1145409453703365 2314 66-67 -2.231428773180035 2314 68-69 -1.8551749223514413 2314 70-71 -2.5331660995990752 2314 72-73 -2.5487740844098283 2314 74-75 -2.0120107493074 2314 76-77 -0.8007135213471201 2314 78-79 -0.507933509656457 2314 80-81 -0.4172957814579057 2314 82-83 -0.4437351121134583 2314 84-85 -0.015731230592017198 2314 86-87 0.33399710521786474 2314 88-89 0.34472179059021 2314 90-91 0.7842331427659097 2314 92-93 1.1959457711225099 2314 94-95 1.0554840994239072 2314 96-97 0.9467796553116195 2314 98-99 0.8602117977242028 2314 100-101 1.0818971732436644 2315 1 -14.065641674443949 2315 2 -7.25029491123864 2315 3 -4.578088006727022 2315 4 -2.753310368567689 2315 5 -3.587903831909994 2315 6 -2.677966156790177 2315 7 -3.128361602986022 2315 8 -3.490010613635718 2315 9 -2.2903860757893426 2315 10-11 -4.643663897949839 2315 12-13 -4.649627997494129 2315 14-15 -3.422566379664705 2315 16-17 -4.632859707697328 2315 18-19 -1.5315402808978362 2315 20-21 -3.1819843690025635 2315 22-23 -4.128857480380262 2315 24-25 -4.756978128361638 2315 26-27 -4.41644839292546 2315 28-29 -2.5043199599894734 2315 30-31 -2.9808409346777722 2315 32-33 -4.101225431383625 2315 34-35 -3.768489425308971 2315 36-37 -4.808498597214882 2315 38-39 -3.3382851597512797 2315 40-41 -4.464711193598433 2315 42-43 -3.22598646422761 2315 44-45 -1.742760011761014 2315 46-47 -1.0393172609457615 2315 48-49 -0.5403779813718081 2315 50-51 -0.5320990099589018 2315 52-53 -0.3109155580069931 2315 54-55 -1.8748375440001972 2315 56-57 -4.121670339446599 2315 58-59 -3.1559177188805414 2315 60-61 -4.045406429958103 2315 62-63 -2.0671474673073185 2315 64-65 -2.983001118116505 2315 66-67 -1.7589040854587523 2315 68-69 -0.9974852569097727 2315 70-71 -1.7714638094043025 2315 72-73 -1.8490276526168117 2315 74-75 -2.503800274550656 2315 76-77 -2.290887026044608 2315 78-79 -1.60023054842285 2315 80-81 -1.1929228278138915 2315 82-83 -0.7592620327520159 2315 84-85 -0.5995064640441434 2315 86-87 -1.1188155473766663 2315 88-89 -1.5740963557307346 2315 90-91 -1.278919714019235 2315 92-93 -0.2582068581041881 2315 94-95 -0.01657823090390309 2315 96-97 -0.8473269795255955 2315 98-99 -0.2933256155999455 2315 100-101 -0.08808085314363368 2316 1 -14.065641674443949 2316 2 -7.213799839847937 2316 3 -5.123283612847338 2316 4 -2.92902009301314 2316 5 -2.118989563653475 2316 6 -1.0817276631898594 2316 7 -4.549366823627775 2316 8 -2.9228681801003766 2316 9 -1.2300926056350576 2316 10-11 -4.4813015543476595 2316 12-13 -3.7420276549282896 2316 14-15 -1.9388876243519633 2316 16-17 -3.2437466776584145 2316 18-19 -2.3727110486415413 2316 20-21 -3.472471352912752 2316 22-23 -3.1012454907470897 2316 24-25 -3.631388928812971 2316 26-27 -3.8760285336915175 2316 28-29 -3.6943396491813587 2316 30-31 -2.588653273551806 2316 32-33 -2.886643512749643 2316 34-35 -4.158516741575625 2316 36-37 -2.3152942887413204 2316 38-39 -3.2427771216916454 2316 40-41 -2.940926112798511 2316 42-43 -6.928525146226299 2316 44-45 -3.5546083675434055 2316 46-47 -4.71263354942505 2316 48-49 -4.2962216256972425 2316 50-51 -3.2823874588664843 2316 52-53 -4.800877931817951 2316 54-55 -4.922550833594986 2316 56-57 -4.66044871954578 2316 58-59 -3.4134168021339697 2316 60-61 -3.342162484229501 2316 62-63 -2.3674960021601628 2316 64-65 -2.533274505423428 2316 66-67 -3.004921853868673 2316 68-69 -2.986798259649529 2316 70-71 -3.1717783165925795 2316 72-73 -3.9879669607844335 2316 74-75 -4.524215536145416 2316 76-77 -4.118545409258797 2316 78-79 -4.09016698162187 2316 80-81 -4.067614910075903 2316 82-83 -3.643368257258011 2316 84-85 -3.3833002887022943 2316 86-87 -3.5146703552578167 2316 88-89 -3.1418815280983416 2316 90-91 -2.4710672287053654 2316 92-93 -2.3078569219234417 2316 94-95 -1.5445382526234166 2316 96-97 -1.8887498579258484 2316 98-99 -1.9553913269674759 2316 100-101 -1.8427015447021624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20946.0 3 6477.0 4 7096.0 5 8434.0 6 9972.0 7 12323.0 8 15298.0 9 18608.0 10 22411.0 11 26201.0 12 30279.0 13 33257.0 14 35716.0 15 39396.0 16 43490.0 17 45344.0 18 42994.0 19 43338.0 20 46613.0 21 50915.0 22 55668.0 23 61192.0 24 67915.0 25 75118.0 26 83308.0 27 92117.0 28 103222.0 29 115906.0 30 130114.0 31 147068.0 32 166503.0 33 190145.0 34 216486.0 35 246104.0 36 251120.0 37 142740.0 38 17004.0 39 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.228561529042686 18.661821123578594 13.284036517166612 22.825580830212107 2 15.091392705577952 21.076400115797323 40.27635391457851 23.55585326404622 3 19.796073063393237 25.883082380300554 29.87150615975626 24.449338396549948 4 12.231751517738028 16.39698363490479 38.912126033658126 32.45913881369906 5 12.87263077057245 38.53568751579977 34.692668502352994 13.899013211274788 6 30.434209712705783 37.062411481168496 18.20782675778892 14.295552048336804 7 27.330999628226788 33.52153892023808 21.88095159218319 17.266509859351935 8 25.538839590041682 34.31582573974037 20.630500109689322 19.51483456052863 9 26.716199177222506 16.89719108856451 20.875973174715455 35.510636559497534 10-11 24.51446713420014 26.5021007726906 28.22468838001768 20.758743713091583 12-13 26.474531645122383 24.239957278247495 28.04117378824688 21.24433728838324 14-15 23.023831489248618 25.350280399148968 27.30360603629721 24.322282075305203 16-17 21.684645361886762 28.566665016597913 27.70256878900473 22.0461208325106 18-19 21.44705776478571 27.938016207244253 29.701496739908386 20.913429288061653 20-21 22.79068577296222 26.54150081937246 29.195118889084842 21.472694518580482 22-23 22.025797682785754 26.704083378028276 29.204201674638107 22.06591726454786 24-25 22.387882402104122 26.642666670104532 29.40433609596659 21.565114831824754 26-27 22.21289975770391 26.441341307846418 29.946964466616194 21.398794467833476 28-29 21.617939488406062 27.43238420335585 29.608855419889558 21.34082088834853 30-31 21.867941167369757 26.88425245811299 29.38995846293712 21.857847911580134 32-33 21.685713438549918 26.98192722158265 29.530731716121117 21.801627623746313 34-35 22.466068780921475 26.72320580876249 29.267025270444357 21.543700139871678 36-37 22.316738105921083 26.430339316359387 29.7772535032729 21.475669074446632 38-39 21.919961263858664 26.975046118837476 29.74138470658846 21.363607910715405 40-41 22.093388258578234 27.036365728488253 29.426423952316295 21.44382206061722 42-43 21.70203514166904 27.272827688589523 28.873356117023043 22.151781052718395 44-45 22.04611919387984 26.972247431966373 28.781751619054774 22.199881755099014 46-47 22.384682707544606 27.329257179313203 28.40478205873404 21.88127805440815 48-49 22.344249819135 27.233811661703623 28.421939992603324 21.999998526558056 50-51 21.825612456653072 27.570137011837076 28.778159154181882 21.82609137732797 52-53 22.20779139422312 27.894835561032266 28.057951617562953 21.83942142718166 54-55 21.71642504574779 27.329462019247508 28.40252013077798 22.551592804226722 56-57 22.190359104701653 27.63769977760281 27.96964715741393 22.20229396028161 58-59 22.66612467561541 27.28026533996683 27.9484202728218 22.10518971159596 60-61 22.191173787338904 27.380190263876102 28.6345863116724 21.794049637112593 62-63 22.077731733786432 27.328974682187823 28.494387260777778 22.09890632324797 64-65 21.90220144868196 27.776738609819105 28.26417384183504 22.0568860996639 66-67 21.852019198831044 27.59307212965766 27.88961997833245 22.665288693178848 68-69 21.705042739291244 27.619284321685196 28.1863554605589 22.489317478464663 70-71 22.11315154477922 27.47181706617615 27.87151988525207 22.543511503792555 72-73 22.117027922162112 27.04449321098971 28.238205420600405 22.600273446247776 74-75 21.64735002498977 27.997257296635492 27.83177029822478 22.52362238014996 76-77 21.96965886906713 27.254274844612652 27.849517216452057 22.926549069868166 78-79 21.887680148254173 27.36752132028741 28.03464667593255 22.71015185552587 80-81 22.01807482499305 27.487130663443015 27.786326734046728 22.708467777517207 82-83 21.94987976042345 27.32939196812523 27.999675799839558 22.721052471611763 84-85 21.807837422909003 27.345922507468558 27.82787302596312 23.018367043659318 86-87 21.731866951564538 27.41397352600896 27.94233727983923 22.91182224258727 88-89 21.838136098134605 27.203953820525502 27.60837875493698 23.349531326402914 90-91 21.7077576413597 27.008595580136745 27.878208196195125 23.40543858230843 92-93 21.85811207098319 27.37732229021103 27.854113521318325 22.91045211748746 94-95 21.731188968046006 27.13536308946588 27.75559364398162 23.377854298506495 96-97 21.248703869788276 27.021903241564765 28.01622192071064 23.713170967936314 98-99 21.55022426417465 27.199753619042845 27.343531136006415 23.906490980776095 100-101 21.59761018072741 26.867774280839885 27.649968660253 23.8846468781797 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3823.0 1 2594.5 2 1253.5 3 1038.0 4 896.0 5 916.5 6 1196.5 7 2459.5 8 3360.0 9 2951.5 10 2541.5 11 2416.0 12 2534.5 13 2739.5 14 2979.5 15 3225.0 16 3517.0 17 3849.0 18 4229.5 19 4717.5 20 5326.0 21 6243.5 22 7436.5 23 9043.0 24 10841.0 25 13332.0 26 16866.0 27 20806.0 28 25020.0 29 29622.0 30 34587.0 31 39717.5 32 44895.5 33 50784.5 34 57108.0 35 62798.5 36 69057.0 37 74418.0 38 77440.5 39 79306.5 40 81735.5 41 85115.0 42 88182.5 43 91526.0 44 94996.0 45 98480.0 46 104980.5 47 122620.0 48 134435.5 49 124468.5 50 109037.5 51 97696.5 52 90705.5 53 85669.5 54 79456.5 55 72470.0 56 65265.0 57 57789.5 58 50938.0 59 44394.0 60 37064.5 61 29926.0 62 23615.0 63 17835.0 64 13210.5 65 9651.0 66 6918.0 67 4928.0 68 3493.5 69 2510.0 70 1726.5 71 1221.0 72 886.5 73 627.5 74 449.0 75 312.0 76 199.0 77 116.0 78 69.0 79 46.0 80 31.0 81 20.0 82 19.5 83 15.0 84 8.0 85 9.5 86 7.0 87 5.0 88 6.5 89 4.5 90 3.0 91 4.0 92 5.5 93 6.0 94 5.5 95 6.5 96 5.5 97 3.0 98 2.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.115940537584464 2 0.08706639538507677 3 0.12025379725621409 4 0.190855125046308 5 0.12161363542812116 6 0.14612747490547287 7 0.15443351346901335 8 0.1524488847856895 9 0.13870349353452077 10-11 0.1271448690733107 12-13 0.1394752935780356 14-15 0.1463296130121077 16-17 0.14162530798496975 18-19 0.1484980036105541 20-21 0.17674220996489412 22-23 0.1940709585609531 24-25 0.22237029348982987 26-27 0.21483605496980424 28-29 0.21422964064989974 30-31 0.22889384147668138 32-33 0.2195036076139177 34-35 0.2045637639144522 36-37 0.1911675203020164 38-39 0.18751065819107712 40-41 0.18741877723351583 42-43 0.18133625784295854 44-45 0.19704790158593882 46-47 0.22411603168349437 48-49 0.22712972709150464 50-51 0.23797168008373665 52-53 0.26461715777651024 54-55 0.2516251903773441 56-57 0.22692758898486975 58-59 0.18376191512257653 60-61 0.19340941566651182 62-63 0.19820560165121104 64-65 0.18611406763614552 66-67 0.1958902015206666 68-69 0.18475422946423847 70-71 0.19826073022574786 72-73 0.21816214563352287 74-75 0.2499713331412409 76-77 0.250191847439388 78-79 0.24456873283663713 80-81 0.25011834267333893 82-83 0.24034220878881787 84-85 0.20932319751612696 86-87 0.22128609819060668 88-89 0.22306858876729568 90-91 0.21086679760315657 92-93 0.20866165462168568 94-95 0.2484461092457235 96-97 0.2773518584945048 98-99 0.2954891595171031 100-101 0.29047245923425674 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2720912.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.53282844893681 #Duplication Level Percentage of deduplicated Percentage of total 1 84.17623893761666 61.89716937276481 2 10.122957458432387 14.88739388373588 3 2.827246920731546 6.236863884148128 4 1.1455338725706503 3.369373829367355 5 0.5743544203638288 2.111695253075098 6 0.3220328576718996 1.4207992126865208 7 0.1996988600951858 1.0279095414579271 8 0.12643071798718833 0.7437446637142263 9 0.09392933011579381 0.6216200385955397 >10 0.39221186440638045 4.701465489373505 >50 0.012317334603369272 0.5978172286472776 >100 0.005400538406780792 0.701257668206514 >500 8.783397324335656E-4 0.4152931552793774 >1k 7.13651032584097E-4 1.0624933067539553 >5k 5.4896233275699766E-5 0.2051034721939458 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5081 0.18673885814756228 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4524 0.16626778080290727 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3038 0.11165373962847751 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2843 0.10448702493869703 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.675238302451531E-5 0.0 0.0 0.0 0.0 7 3.675238302451531E-5 0.0 0.0 0.0 0.0 8 7.350476604903063E-5 0.0 0.0 0.0 0.0 9 7.350476604903063E-5 0.0 0.0 0.0 0.0 10-11 7.350476604903063E-5 0.0 0.0 0.0 0.0 12-13 1.4700953209806125E-4 1.8376191512257656E-5 0.0 0.0 0.0 14-15 1.8376191512257654E-4 3.675238302451531E-5 0.0 0.0 0.0 16-17 2.2051429814709186E-4 3.675238302451531E-5 0.0 1.8376191512257656E-5 0.0 18-19 2.572666811716072E-4 3.675238302451531E-5 0.0 1.1025714907354593E-4 0.0 20-21 2.940190641961225E-4 3.675238302451531E-5 0.0 1.8376191512257657E-4 0.0 22-23 3.307714472206378E-4 3.675238302451531E-5 0.0 2.7564287268386483E-4 0.0 24-25 4.042762132696684E-4 3.675238302451531E-5 0.0 4.77780979318699E-4 0.0 26-27 4.042762132696684E-4 3.675238302451531E-5 0.0 7.901762350270791E-4 0.0 28-29 4.961571708309567E-4 3.675238302451531E-5 0.0 0.0028666858759121942 0.0 30-31 5.512857453677297E-4 3.675238302451531E-5 0.0 0.009096214798567539 0.0 32-33 5.512857453677297E-4 3.675238302451531E-5 0.0 0.02192279647412338 0.0 34-35 5.696619368799874E-4 5.5128574536772966E-5 0.0 0.040978907072334564 0.0 36-37 6.615428944412756E-4 7.350476604903063E-5 0.0 0.07089534685429003 0.0 38-39 8.453048095638521E-4 7.350476604903063E-5 0.0 0.11729523042274062 0.0 40-41 8.636810010761097E-4 7.350476604903063E-5 0.0 0.16794001423052268 0.0 42-43 9.739381501496557E-4 7.350476604903063E-5 0.0 0.2210104553179228 0.0 44-45 0.0010290667246864286 7.350476604903063E-5 0.0 0.2860254208882904 0.0 46-47 0.0011393238737599748 1.1025714907354593E-4 0.0 0.3619742204084513 0.0 48-49 0.00117607625678449 1.1025714907354593E-4 0.0 0.4420392868273579 0.0 50-51 0.00117607625678449 1.1025714907354593E-4 0.0 0.5331484443451313 0.0 52-53 0.00117607625678449 1.1025714907354593E-4 0.0 0.6238900780326597 0.0 54-55 0.00117607625678449 1.1025714907354593E-4 0.0 0.7215044073457723 0.0 56-57 0.0012128286398090053 1.1025714907354593E-4 0.0 0.8453048095638521 0.0 58-59 0.0012128286398090053 1.1025714907354593E-4 0.0 0.9807189648176787 1.8376191512257656E-5 60-61 0.0012128286398090053 1.1025714907354593E-4 0.0 1.1324511781343902 3.675238302451531E-5 62-63 0.0012128286398090053 1.1025714907354593E-4 0.0 1.28791375832809 3.675238302451531E-5 64-65 0.0012128286398090053 1.1025714907354593E-4 0.0 1.4435049718623756 3.675238302451531E-5 66-67 0.0012128286398090053 1.1025714907354593E-4 0.0 1.6208168437641497 3.675238302451531E-5 68-69 0.0012128286398090053 1.1025714907354593E-4 0.0 1.829037469789541 3.675238302451531E-5 70-71 0.0012312048313212628 1.1025714907354593E-4 0.0 2.0584274684370536 3.675238302451531E-5 72-73 0.0012679572143457782 1.1025714907354593E-4 3.675238302451531E-5 2.2888832861922768 3.675238302451531E-5 74-75 0.0012863334058580357 1.1025714907354593E-4 3.675238302451531E-5 2.5298686616840236 3.675238302451531E-5 76-77 0.0013047095973702935 1.1025714907354593E-4 3.675238302451531E-5 2.782394285445468 3.675238302451531E-5 78-79 0.0013230857888825512 1.1025714907354593E-4 3.675238302451531E-5 3.0856749501637686 3.675238302451531E-5 80-81 0.0015803524700541583 1.1025714907354593E-4 3.675238302451531E-5 3.416648535491041 3.675238302451531E-5 82-83 0.0015803524700541583 1.1025714907354593E-4 3.675238302451531E-5 3.745104582581135 3.675238302451531E-5 84-85 0.0016354810445909313 1.1025714907354593E-4 3.675238302451531E-5 4.094013330824371 3.675238302451531E-5 86-87 0.001708985810639962 1.1025714907354593E-4 3.675238302451531E-5 4.459221760938979 3.675238302451531E-5 88-89 0.0017273620021522195 1.1025714907354593E-4 3.675238302451531E-5 4.858352640585215 3.675238302451531E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10095 0.0 31.027143 1 TACATGG 11680 0.0 25.682056 2 ACATGGG 11660 0.0 24.794752 3 GAGTACT 9545 0.0 22.126871 12-13 AGTACTT 10050 0.0 21.296791 12-13 CATGGGA 8490 0.0 21.08504 4 GTACTTT 10255 0.0 20.805475 14-15 GTATAAT 1315 0.0 19.912937 1 GTGTAGC 1425 0.0 19.789322 1 GTATAGG 815 0.0 19.771976 1 CATGGGG 3990 0.0 19.532324 4 GTATAGA 935 0.0 18.311548 1 AGTACAT 8565 0.0 17.70421 2 GTATCAA 29765 0.0 17.45949 1 TACTTTT 12385 0.0 17.341644 14-15 ACTTTTT 12990 0.0 17.265507 16-17 AGAGTAC 20190 0.0 16.479794 10-11 GTATAGC 925 8.367351E-11 16.33192 1 ATGGGAT 3740 0.0 15.898857 5 GAGTACA 13065 0.0 15.879819 1 >>END_MODULE