FastQCFastQC Report
Fri 27 May 2016
SRR939634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720912
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT135120.49659819942725086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116200.4270626907448679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84880.311954227112086No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69310.2547307667429156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64280.23624431808158441No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55970.2057030877882122No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50610.18600381048707199No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA44930.1651284569291473No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38120.14010008408945235No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA37680.13848297923637368No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35640.13098549309937257No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31600.11613753035746838No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30970.11382213022692392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG114150.027.4161681
TACATGG114750.026.4437852
ACATGGG115500.025.4920063
CATGGGA82050.022.7728824
AGTACTT97700.019.71530712-13
CATGGGG38750.019.4827234
ATGGGAG34650.019.0645355
GTGTAGC16450.018.6516231
GAGTACT95350.018.4456612-13
ATGGGAT34850.018.1427635
CGCATAG6600.018.03198666-67
AGTACAT82700.017.1761682
GTAGCCC15450.016.7971523
GTACTTT103400.016.73599614-15
AGAGTAC193250.016.05507910-11
TACTTTT119050.015.98159714-15
TGCGCGA1257.202987E-515.54430866-67
TAGCCCT18800.015.0589544
GTATCAA297850.014.833631
ACTTTTT120250.014.72417216-17
Produced by FastQC (version 0.11.5)