##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939634_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2720912 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.479666744091688 31.0 31.0 34.0 30.0 34.0 2 31.68195296283011 31.0 31.0 34.0 30.0 34.0 3 31.737981603227155 31.0 31.0 34.0 30.0 34.0 4 35.52953090728403 37.0 35.0 37.0 33.0 37.0 5 35.291852511216824 37.0 35.0 37.0 33.0 37.0 6 35.303106090898936 37.0 35.0 37.0 32.0 37.0 7 35.21006155289109 37.0 35.0 37.0 32.0 37.0 8 35.1736873518879 37.0 35.0 37.0 32.0 37.0 9 36.76862169743086 39.0 35.0 39.0 32.0 39.0 10-11 36.64613225271526 39.0 35.0 39.0 32.0 39.0 12-13 36.58799861958049 39.0 35.0 39.0 32.0 39.0 14-15 37.63402638527082 39.0 36.0 41.0 32.0 41.0 16-17 37.6031163448138 39.0 36.0 41.0 32.0 41.0 18-19 37.5979326049501 39.0 36.0 41.0 32.0 41.0 20-21 37.53877284528129 39.0 36.0 41.0 32.0 41.0 22-23 37.40432435889143 39.0 36.0 41.0 32.0 41.0 24-25 37.276636289597015 39.0 36.0 41.0 31.0 41.0 26-27 36.98340115373081 39.0 36.0 40.0 31.0 41.0 28-29 36.80077672486284 39.0 36.0 40.0 30.0 41.0 30-31 36.5692877976208 38.0 35.0 40.0 30.0 41.0 32-33 36.39134304968334 38.0 35.0 40.0 30.0 41.0 34-35 36.35594811592584 38.0 35.0 40.0 30.0 41.0 36-37 36.26289126586968 38.0 35.0 40.0 30.0 41.0 38-39 36.28645321862669 38.0 35.0 40.0 30.0 41.0 40-41 36.1923096006045 38.0 35.0 40.0 30.0 41.0 42-43 36.00720530469196 38.0 35.0 40.0 29.5 41.0 44-45 35.79018689321816 38.0 35.0 40.0 28.5 41.0 46-47 35.49710354469384 38.0 34.5 40.0 27.5 41.0 48-49 35.19495319951545 38.0 34.0 40.0 26.5 41.0 50-51 34.95485796674056 38.0 34.0 40.0 26.0 41.0 52-53 34.737387868479395 38.0 33.5 40.0 26.0 41.0 54-55 34.47674088687911 38.0 33.0 40.0 25.0 41.0 56-57 33.6834949090599 37.5 32.5 40.0 21.5 41.0 58-59 30.614570776269133 35.5 27.0 39.0 8.0 40.5 60-61 30.337356555449055 34.5 26.0 39.0 10.5 40.0 62-63 32.1594141964165 35.5 30.5 39.0 18.5 40.0 64-65 32.22105896846352 36.0 31.0 39.0 16.0 40.0 66-67 27.324332797238576 32.0 18.5 37.0 4.0 39.5 68-69 19.84493912335276 20.0 8.0 32.5 2.0 37.0 70-71 17.89223006844764 15.5 7.5 29.0 2.0 35.0 72-73 19.613781702605596 20.0 7.5 31.0 2.0 35.5 74-75 21.057709878158498 23.5 9.5 32.5 2.0 36.0 76-77 22.86672685481927 27.5 11.5 33.0 2.0 35.5 78-79 25.026200590096263 30.5 13.0 34.5 2.0 36.5 80-81 26.62342019881569 32.0 22.5 35.0 2.0 37.0 82-83 27.76017434595459 33.0 26.5 35.0 2.0 36.0 84-85 27.80426562858336 34.0 27.0 35.0 2.0 36.0 86-87 27.542012200321068 33.5 26.5 35.0 2.0 36.0 88-89 27.099736228147034 33.0 26.0 35.0 2.0 35.5 90-91 25.264244304850727 32.5 16.0 34.5 2.0 35.0 92-93 23.669977933869234 31.5 2.0 34.0 2.0 35.0 94-95 19.933347164480146 28.0 2.0 34.0 2.0 35.0 96-97 17.230464454565237 18.5 2.0 33.0 2.0 35.0 98-99 17.501372885267884 21.5 2.0 33.0 2.0 35.0 100-101 16.733575360026343 17.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2920630490220866 1101 2 0.26206406372266144 1101 3 0.23486096986455252 1101 4 0.2212569381946139 1101 5 0.3321631572617392 1101 6 0.3592527812784567 1101 7 0.368572864562438 1101 8 0.36533291627545594 1101 9 0.36609842290824446 1101 10-11 0.2910156617023034 1101 12-13 0.2585746837775673 1101 14-15 0.49120962985685424 1101 16-17 0.46337286714432224 1101 18-19 0.39117827984997433 1101 20-21 0.45082882681134606 1101 22-23 0.4271981716506801 1101 24-25 0.3996226652112682 1101 26-27 0.4994552767528688 1101 28-29 0.5446545376123311 1101 30-31 0.33270119418532573 1101 32-33 0.4495397447675131 1101 34-35 0.2791058686963055 1101 36-37 0.12539993805312832 1101 38-39 0.17987753284329244 1101 40-41 0.3014060786220796 1101 42-43 0.4263141520760243 1101 44-45 0.3830874825042656 1101 46-47 0.25444216103534245 1101 48-49 0.23916621797945936 1101 50-51 0.0873978708151526 1101 52-53 -0.02925701861380503 1101 54-55 0.00957728281868242 1101 56-57 -3.426214997697855 1101 58-59 -10.238115636909548 1101 60-61 -12.078521311971244 1101 62-63 -4.046921410450274 1101 64-65 -1.22873821206273 1101 66-67 -2.0641364001539237 1101 68-69 -4.745598930528132 1101 70-71 -4.53070082622801 1101 72-73 -3.340587453428764 1101 74-75 -6.103392806072652 1101 76-77 -4.742014918395 1101 78-79 1.293746408490616 1101 80-81 1.8948149324192975 1101 82-83 0.6917478369966581 1101 84-85 0.3308645711654741 1101 86-87 0.3582386275686531 1101 88-89 0.2440757288338915 1101 90-91 1.6911699633219008 1101 92-93 1.0533592983583304 1101 94-95 -8.674595338282316 1101 96-97 -9.663141571505447 1101 98-99 -8.759035816636034 1101 100-101 -9.593760709270066 1102 1 0.2036690619509116 1102 2 0.2772588511495542 1102 3 0.25525464910660745 1102 4 0.2100767555493377 1102 5 0.30861422953317685 1102 6 0.3153743053746396 1102 7 0.3846376967955649 1102 8 0.31971862616361335 1102 9 0.3442382799387147 1102 10-11 0.36865380002019066 1102 12-13 0.37839503128243734 1102 14-15 0.4960996237002746 1102 16-17 0.4842026376406139 1102 18-19 0.3637034619822046 1102 20-21 0.3105998361488318 1102 22-23 0.3500495671426975 1102 24-25 0.35382239500571444 1102 26-27 0.14345517414315623 1102 28-29 0.2629866168270212 1102 30-31 0.3642087873036317 1102 32-33 0.2534205293897358 1102 34-35 0.270385112462769 1102 36-37 0.09911795802781853 1102 38-39 0.25377469226457805 1102 40-41 0.2973102411558557 1102 42-43 0.3006193988523691 1102 44-45 0.212382340047796 1102 46-47 0.11243567097137941 1102 48-49 0.20014353268342688 1102 50-51 0.21512451000521793 1102 52-53 0.04441033781811399 1102 54-55 -0.19097552697712672 1102 56-57 -3.5512075157400638 1102 58-59 -9.894846619910538 1102 60-61 -8.10315191702648 1102 62-63 -2.4362728743487594 1102 64-65 -0.8269784555897672 1102 66-67 -2.073023897161139 1102 68-69 -4.9316538438229305 1102 70-71 -4.890209967042731 1102 72-73 -3.636599356154763 1102 74-75 -4.014637547325176 1102 76-77 -3.9959012610430165 1102 78-79 1.7932803894052078 1102 80-81 2.040582709551014 1102 82-83 0.6235562048182537 1102 84-85 0.4023878122640845 1102 86-87 0.22926143257600629 1102 88-89 0.020339538390278022 1102 90-91 1.4243419713938614 1102 92-93 1.011797022817067 1102 94-95 -8.8859670590471 1102 96-97 -9.947641947741038 1102 98-99 -8.906918850121002 1102 100-101 -9.50841294787184 1103 1 0.23720940024808712 1103 2 0.23518350182544268 1103 3 0.2232762871335936 1103 4 0.2918255276911239 1103 5 0.2659805512547848 1103 6 0.3051692209865422 1103 7 0.19993063459374127 1103 8 0.303775606232378 1103 9 0.3389766954533826 1103 10-11 0.306183281030286 1103 12-13 0.35491754442993795 1103 14-15 0.46006697632626015 1103 16-17 0.4653699615096727 1103 18-19 0.4335261311204519 1103 20-21 0.40446918251863195 1103 22-23 0.47289732410332164 1103 24-25 0.49916765383864004 1103 26-27 0.5169998725265685 1103 28-29 0.4581236207702375 1103 30-31 0.4629533617633541 1103 32-33 0.35521250931368087 1103 34-35 0.30453136309893836 1103 36-37 0.3036705242204434 1103 38-39 0.3935735128172908 1103 40-41 0.38090480899527535 1103 42-43 0.26447697350656085 1103 44-45 0.35037751757519686 1103 46-47 0.2581238405006303 1103 48-49 0.2586704493628531 1103 50-51 0.3094599925214325 1103 52-53 0.31425816925708006 1103 54-55 0.15571791913572497 1103 56-57 0.5637795436481383 1103 58-59 -8.357130839605095 1103 60-61 -7.519600277675437 1103 62-63 -2.252206377603045 1103 64-65 -0.5104669693744697 1103 66-67 -2.047644885157716 1103 68-69 -3.661042738688476 1103 70-71 -4.10473176954272 1103 72-73 -2.983541580615931 1103 74-75 -3.7046102023952905 1103 76-77 -0.48358259511561386 1103 78-79 3.236323724421201 1103 80-81 2.700557531733974 1103 82-83 0.9375597363329327 1103 84-85 0.6574229877640221 1103 86-87 0.5985426338288988 1103 88-89 0.5246481621546586 1103 90-91 1.8314695486547983 1103 92-93 1.3787384921506067 1103 94-95 -7.6454755899204425 1103 96-97 -8.790874737037672 1103 98-99 -7.961767234975419 1103 100-101 -9.30034270238295 1104 1 0.24541273350298098 1104 2 0.2005659440784875 1104 3 0.21537646240692254 1104 4 0.08601511690580566 1104 5 0.3171626657337967 1104 6 0.19210033859375386 1104 7 0.2857666165102444 1104 8 0.29116501033090003 1104 9 0.3213740864186718 1104 10-11 0.2083688334170688 1104 12-13 0.19633338957403623 1104 14-15 0.3604348301806368 1104 16-17 0.24478359416643514 1104 18-19 0.2739891948030859 1104 20-21 0.2802007848285868 1104 22-23 0.2730916125763443 1104 24-25 0.27964932275853016 1104 26-27 0.30512846348537437 1104 28-29 0.40212901737706375 1104 30-31 0.3624744533920605 1104 32-33 0.3337399451435843 1104 34-35 0.23658929008635 1104 36-37 0.13219857997189166 1104 38-39 0.14014351220748722 1104 40-41 0.14636476674446186 1104 42-43 0.194080011172737 1104 44-45 0.19540257126166694 1104 46-47 0.14545518704866822 1104 48-49 0.04611352095518839 1104 50-51 0.08413875297185314 1104 52-53 0.13529928068278707 1104 54-55 0.11376453875413972 1104 56-57 0.5477769647745134 1104 58-59 -8.009299249263421 1104 60-61 -7.428347826138751 1104 62-63 -1.9639642397896822 1104 64-65 -0.22086840526458573 1104 66-67 -1.399091187426908 1104 68-69 -3.0787997081880825 1104 70-71 -3.711311591526556 1104 72-73 -2.642251680757301 1104 74-75 -3.358450249478963 1104 76-77 -0.0959091530859979 1104 78-79 3.7036679653373916 1104 80-81 3.061297406833699 1104 82-83 1.4608645482145768 1104 84-85 1.000596816548775 1104 86-87 1.0167994625177563 1104 88-89 0.9136877721050816 1104 90-91 2.4542571561953714 1104 92-93 2.0432406205057454 1104 94-95 -6.457085923430643 1104 96-97 -7.9478055990853935 1104 98-99 -5.782724783507051 1104 100-101 -5.291371805042717 1105 1 0.21626188799733015 1105 2 0.24129331839290202 1105 3 0.23171929413818404 1105 4 0.25204608647216276 1105 5 0.33488521673209704 1105 6 0.2251394838519687 1105 7 0.1511442148865143 1105 8 0.2794801564557119 1105 9 0.30533342460952895 1105 10-11 0.3147027140770575 1105 12-13 0.3134598676410647 1105 14-15 0.448242273823098 1105 16-17 0.44963912273574635 1105 18-19 0.41393947929892505 1105 20-21 0.3503797492409504 1105 22-23 0.4211990596395978 1105 24-25 0.3016720938277544 1105 26-27 0.34508758502610704 1105 28-29 0.44430167613030136 1105 30-31 0.41483394437827315 1105 32-33 0.26574886200694436 1105 34-35 0.3949534105438204 1105 36-37 0.4177562830243744 1105 38-39 0.5719215256397945 1105 40-41 0.5092979244771598 1105 42-43 0.5557374987153523 1105 44-45 0.5358654043612461 1105 46-47 0.5024646740439636 1105 48-49 0.43946321707487357 1105 50-51 0.4653289603858468 1105 52-53 0.630142437389253 1105 54-55 0.48860917780361746 1105 56-57 0.9881710969766999 1105 58-59 -8.168827864184912 1105 60-61 -7.319987126472846 1105 62-63 -1.8148106006375784 1105 64-65 0.042357230147452185 1105 66-67 -1.3010912102321832 1105 68-69 -3.0129551717623535 1105 70-71 -3.903202320041135 1105 72-73 -2.785064027534343 1105 74-75 -3.520272220082962 1105 76-77 0.05181345492549738 1105 78-79 4.244401040971095 1105 80-81 3.6124681727489296 1105 82-83 2.0694561513115346 1105 84-85 1.613410246576482 1105 86-87 1.5600525212755514 1105 88-89 1.4784690124841084 1105 90-91 2.8018936742856546 1105 92-93 2.42502519045523 1105 94-95 -6.002344421247038 1105 96-97 -7.722932738505454 1105 98-99 -5.418027067455558 1105 100-101 -4.799757020381547 1106 1 0.06463296978538224 1106 2 0.09285453689069811 1106 3 0.1481159707859092 1106 4 0.13604881387283285 1106 5 0.18105957625397906 1106 6 0.09825177802299834 1106 7 0.23674407317815138 1106 8 0.25689635682745404 1106 9 0.16348893497801953 1106 10-11 0.17457340519650444 1106 12-13 0.1596736580000453 1106 14-15 0.11925138780611633 1106 16-17 0.20924314007076106 1106 18-19 0.22145317696510602 1106 20-21 0.14554868307964597 1106 22-23 0.1893478000366713 1106 24-25 0.1830018957757602 1106 26-27 0.15997679044014035 1106 28-29 0.3002764792187662 1106 30-31 0.22880641316118755 1106 32-33 0.22848878348498403 1106 34-35 0.2663308692081685 1106 36-37 0.24260244209686022 1106 38-39 0.3314822297168192 1106 40-41 0.2741097241248269 1106 42-43 0.30120768414948174 1106 44-45 0.3412503221267187 1106 46-47 0.2769077892984768 1106 48-49 0.30723633325146693 1106 50-51 0.33757523204854323 1106 52-53 0.41318183910819073 1106 54-55 0.2976816674545404 1106 56-57 0.8759738651427895 1106 58-59 -8.063556992532895 1106 60-61 -7.213172143240051 1106 62-63 -1.7847772846133765 1106 64-65 -0.08584673768669404 1106 66-67 0.7282256778133593 1106 68-69 -2.2493186537115264 1106 70-71 -2.263840759890302 1106 72-73 -0.9692916926949557 1106 74-75 -0.9806556334103398 1106 76-77 2.341176713635466 1106 78-79 4.586177588237138 1106 80-81 3.448092660148049 1106 82-83 1.862134269502132 1106 84-85 1.440680734839436 1106 86-87 1.2397770635138627 1106 88-89 1.2350676720782978 1106 90-91 2.58256346468346 1106 92-93 2.164706322375036 1106 94-95 -5.987140254438824 1106 96-97 -7.454882757189605 1106 98-99 -5.188687006210367 1106 100-101 -4.682281880007766 1107 1 0.0804731771829914 1107 2 0.11107201076010043 1107 3 0.17910732802518936 1107 4 0.006308812846349099 1107 5 0.14779586880683127 1107 6 0.147661251042571 1107 7 0.09454601180154043 1107 8 0.12232471716170323 1107 9 0.11773028535417751 1107 10-11 0.09864577951481834 1107 12-13 0.18437469439130183 1107 14-15 0.2246140531327825 1107 16-17 0.1878202893323433 1107 18-19 0.1468790188315836 1107 20-21 0.1722629420234867 1107 22-23 0.22324190025736357 1107 24-25 0.14730422649235209 1107 26-27 0.20373576471953925 1107 28-29 0.2103739833097933 1107 30-31 0.11360376005684714 1107 32-33 0.11728989734002226 1107 34-35 0.04215662227181127 1107 36-37 0.22302809895742115 1107 38-39 0.25547057553706054 1107 40-41 0.08389158423409526 1107 42-43 0.08615375193804198 1107 44-45 -0.009840616044300532 1107 46-47 0.2842642813951386 1107 48-49 0.22228871380029602 1107 50-51 0.1774139904319938 1107 52-53 0.20272864620429942 1107 54-55 0.12085819533493236 1107 56-57 0.6047199069111571 1107 58-59 -8.141523144803514 1107 60-61 -7.23710301786452 1107 62-63 -1.9738997119866113 1107 64-65 -0.3746190861041754 1107 66-67 0.7284072820176455 1107 68-69 -2.6529376680238173 1107 70-71 -2.4969507930600727 1107 72-73 -1.0478646644033738 1107 74-75 -0.9819956731090294 1107 76-77 2.275190948349497 1107 78-79 4.326130751898159 1107 80-81 3.315631460665113 1107 82-83 1.7120640269295215 1107 84-85 1.339387901491584 1107 86-87 1.1510592620049316 1107 88-89 0.9868003420957585 1107 90-91 2.397560761209764 1107 92-93 2.0440125740236788 1107 94-95 -6.679500046802495 1107 96-97 -8.289450875774824 1107 98-99 -6.061617774605626 1107 100-101 -5.387567260600763 1108 1 -0.09680271635578919 1108 2 -0.1090255759160712 1108 3 -0.10799339577608436 1108 4 -0.16271311184270587 1108 5 -0.17157958209552504 1108 6 -0.14633089693749923 1108 7 -0.07028834876716417 1108 8 -0.06556699097341578 1108 9 -0.0650187754846101 1108 10-11 -0.12299444887474209 1108 12-13 -0.18682513192734973 1108 14-15 -0.25391100193726857 1108 16-17 -0.18572255787112368 1108 18-19 -0.16177681683452505 1108 20-21 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2 -0.35055406697258107 2316 3 -0.5015012373925174 2316 4 -0.3878062844262047 2316 5 -0.5401885611873212 2316 6 -0.49995677658625937 2316 7 -0.5054537404405863 2316 8 -0.5391391467327296 2316 9 -0.6257227409350747 2316 10-11 -0.6624692300289325 2316 12-13 -0.5366938582984062 2316 14-15 -0.6419587020372717 2316 16-17 -0.5849723372130882 2316 18-19 -0.6454512307045164 2316 20-21 -0.6773402005374081 2316 22-23 -0.8399877678022634 2316 24-25 -0.5994860420738064 2316 26-27 -0.6730552009471893 2316 28-29 -0.6681106889803061 2316 30-31 -0.5653946607119309 2316 32-33 -0.5381613333803372 2316 34-35 -0.5877496452929591 2316 36-37 -0.27379620816234507 2316 38-39 -0.5330622214680432 2316 40-41 -0.6200765509498112 2316 42-43 -0.6046164338251785 2316 44-45 -0.507666315450706 2316 46-47 -0.538597002236159 2316 48-49 -0.4797562571821885 2316 50-51 -0.5151721479427991 2316 52-53 -0.4814733093486083 2316 54-55 -0.41377570918166384 2316 56-57 0.38822823050591637 2316 58-59 3.5761342887154512 2316 60-61 3.582453795099795 2316 62-63 0.957196683057461 2316 64-65 0.4587489896403625 2316 66-67 -1.6705634754581382 2316 68-69 -2.996619172121342 2316 70-71 -3.073449190460778 2316 72-73 -0.2760703331711376 2316 74-75 6.035411907854019 2316 76-77 6.053671983103513 2316 78-79 5.118371527055967 2316 80-81 3.3910001947698944 2316 82-83 1.6560988450194962 2316 84-85 1.3826188916773283 2316 86-87 1.2424479077344799 2316 88-89 1.3326564846385232 2316 90-91 2.894227742078467 2316 92-93 4.494351422334706 2316 94-95 7.12307741216345 2316 96-97 4.277988254901352 2316 98-99 6.5769621583353235 2316 100-101 6.548441046946044 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 19.0 4 38.0 5 99.0 6 276.0 7 590.0 8 1416.0 9 2415.0 10 4110.0 11 6497.0 12 9281.0 13 12599.0 14 15914.0 15 20580.0 16 27572.0 17 36426.0 18 38877.0 19 38287.0 20 39977.0 21 41268.0 22 41769.0 23 42473.0 24 44686.0 25 47166.0 26 59237.0 27 82755.0 28 117387.0 29 156049.0 30 190742.0 31 211778.0 32 222472.0 33 222004.0 34 227756.0 35 242481.0 36 244719.0 37 188918.0 38 77015.0 39 5259.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.167143588175094 19.66213076570165 14.12115325796699 23.04957238815627 2 15.303543841219094 21.861522859655977 39.28288794407367 23.55204535505126 3 19.637135571754406 25.839922437599828 30.370545693236 24.15239629740976 4 12.43976284422282 17.09287180180763 38.38907689774605 32.078288456223504 5 12.685791578036875 38.81782569425444 34.81473799415705 13.681644733551634 6 30.552145751130507 37.54175070711585 17.67866803483538 14.227435506918267 7 27.15141099748908 33.6195731431226 21.66394944048172 17.565066418906603 8 25.378393764730834 34.95802321944615 20.32986035181625 19.33372266400677 9 26.07335334623097 16.804402347448207 20.922911141558416 36.19933316476241 10-11 24.43414193476305 26.717549115884676 28.15120444909648 20.697104500255797 12-13 26.130301163727452 24.29236961724598 28.22217330071682 21.35515591830974 14-15 22.569596878093527 25.55283607289716 27.299720331662137 24.577846717347175 16-17 21.401375972720906 28.768796780049627 27.721427176150343 22.10840007107912 18-19 21.186187303661193 28.089797410969336 29.729823100974766 20.9941921843947 20-21 22.488103253614963 26.66947699888861 29.21514918527317 21.627270562223256 22-23 21.77422496574678 26.82828404593754 29.193373398331147 22.204117589984534 24-25 22.13557635783182 26.853704616048418 29.348366786914447 21.66235223920531 26-27 22.075271605253615 26.5754997461324 29.821348142613502 21.527880506000482 28-29 21.455874837529556 27.54611146873649 29.53752637704095 21.460487316693 30-31 21.77101800465567 26.958706703639063 29.34297791230676 21.927297379398507 32-33 21.646358888183816 27.049913158312094 29.39404147120166 21.909686482302433 34-35 22.309514980022836 26.849022201460787 29.204934327676906 21.636528490839467 36-37 22.192424751833524 26.59190344221784 29.65682289524737 21.55884891070127 38-39 21.86752864219727 27.047639400363995 29.659037575090597 21.425794382348137 40-41 21.946676676844213 27.04864861683538 29.41134601636561 21.593328689954795 42-43 21.568948783016584 27.36420775927284 28.870499824625195 22.19634363308538 44-45 21.91815718944757 27.07426271520483 28.785159633375816 22.22242046197178 46-47 22.232321723770333 27.35634940662261 28.443159588391566 21.96816928121549 48-49 22.120272637693546 27.29203578231798 28.511059952412353 22.076631627576116 50-51 21.743308053873935 27.54733462305163 28.809995807189566 21.899361515884866 52-53 22.126761254848056 27.878617043634822 28.08997957717564 21.904642124341482 54-55 21.696660423023378 27.317060883979284 28.455477822564333 22.530800870433005 56-57 22.045418394826875 27.546231637675255 28.198076351964517 22.210273615533353 58-59 21.433168363549832 26.989120728201026 29.633127580621228 21.944583327627917 60-61 21.387621297018438 27.272953553590096 29.584487994291685 21.75493715509978 62-63 22.067924886128456 27.25829149865721 28.554624344271545 22.119159270942788 64-65 21.770286744634863 27.759529823919188 28.355721532916334 22.114461898529616 66-67 21.59088798119756 27.309919206885507 28.641467457977992 22.457725353938933 68-69 20.962801426932725 27.037349838020447 29.97224739646488 22.027601338581952 70-71 21.86959280220916 27.09061699353831 28.856846792637665 22.18294341161487 72-73 21.385795956239992 26.881706550532037 29.410309941783026 22.322187551444948 74-75 21.210944892747502 27.620935288601693 28.894726763819705 22.273393054831097 76-77 20.318772593716545 27.501336950117366 29.26677749762551 22.913112958540573 78-79 21.56036211373331 27.414542759249876 28.416474034958107 22.608621092058705 80-81 21.705911300980354 27.645963512775456 28.04048408691034 22.60764109933385 82-83 21.89331018908588 27.47062422027365 28.051901085171433 22.584164505469044 84-85 21.753762068950945 27.490222360331313 27.830333350300908 22.92568222041684 86-87 21.69508525208095 27.600434713577116 27.91165846831457 22.792821566027364 88-89 21.749898884987623 27.447658624692494 27.594943826728652 23.207498663591227 90-91 21.548567845574627 27.31510937160942 27.963556992944916 23.17276578987104 92-93 21.526091111772192 27.752347808436333 27.971785481702007 22.749775598089467 94-95 20.450127158074988 27.68770399684975 28.549366658004597 23.312802187070663 96-97 19.805732026009494 27.776659076517298 28.720708032046744 23.696900865426464 98-99 20.770804413081695 27.58503362993407 28.01139680585433 23.63276515112991 100-101 19.965459772886735 27.59228636087885 28.644588047920962 23.797665818313455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1020.0 1 740.0 2 486.5 3 561.0 4 631.0 5 721.5 6 920.0 7 1378.0 8 1963.5 9 2367.5 10 2475.5 11 2560.5 12 2800.0 13 2940.0 14 3103.0 15 3410.5 16 3691.0 17 3998.5 18 4458.5 19 4911.5 20 5486.5 21 6254.5 22 7270.0 23 8661.0 24 10536.0 25 12960.5 26 16061.0 27 19744.5 28 23874.0 29 28567.5 30 33717.5 31 39186.5 32 44900.5 33 50854.5 34 57210.5 35 63538.0 36 69769.5 37 75312.0 38 79268.0 39 82502.5 40 85719.5 41 88995.0 42 92155.0 43 95881.5 44 100312.5 45 105284.5 46 111734.5 47 120869.0 48 129540.5 49 124878.5 50 110724.5 51 100255.0 52 92407.0 53 85742.0 54 79078.5 55 71852.5 56 64303.0 57 56381.5 58 48690.0 59 41403.0 60 34119.5 61 26987.0 62 20746.0 63 15642.0 64 11422.5 65 8062.5 66 5580.5 67 3868.0 68 2658.0 69 1736.5 70 1200.0 71 864.5 72 577.0 73 367.0 74 247.5 75 164.0 76 81.0 77 36.0 78 18.0 79 10.0 80 4.5 81 1.5 82 1.0 83 2.0 84 1.0 85 0.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02528563952086653 2 0.0122385435471636 3 2.2051429814709184E-4 4 0.0 5 0.06380213693055858 6 0.0 7 0.0 8 1.1025714907354592E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 3.859000217574107E-4 16-17 1.8376191512257656E-5 18-19 3.4914763873289547E-4 20-21 0.0 22-23 0.0 24-25 9.188095756128827E-5 26-27 2.021381066348342E-4 28-29 0.0011944524482967475 30-31 0.005273966964017947 32-33 0.0057517479433366455 34-35 0.0020213810663483422 36-37 0.08725015730019935 38-39 0.08070823312183562 40-41 0.08620271438400065 42-43 0.08998820983552575 44-45 0.09344293383983017 46-47 0.08842623355698383 48-49 0.07861334728943825 50-51 0.0724756993243442 52-53 0.06065980818196252 54-55 0.0494687075509976 56-57 0.06834105623408622 58-59 0.6909815532439124 60-61 0.33360138071352546 62-63 0.0030504477910347706 64-65 0.0021683905984464036 66-67 1.0785354322374263 68-69 2.3272895264528954 70-71 2.3046500585097935 72-73 1.7697007473964612 74-75 1.251473770559283 76-77 0.7277523124599399 78-79 0.5581768171848263 80-81 0.037799825940713995 82-83 2.021381066348342E-4 84-85 0.0013047095973702935 86-87 0.0014700953209806124 88-89 1.8376191512257656E-5 90-91 0.3269675755776004 92-93 0.05369523159881686 94-95 0.9280527999435484 96-97 1.624345072534503 98-99 0.6246618780761745 100-101 0.5542810645842277 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2720912.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.564075659650754 #Duplication Level Percentage of deduplicated Percentage of total 1 72.19307658472184 37.22569263117558 2 14.739052407919193 15.200112270270058 3 5.5058439293468036 8.517112588292022 4 2.61936713734214 5.402609810012517 5 1.4279073594220646 3.681436155810573 6 0.8569202529721592 2.651178045512609 7 0.5454959164227555 1.9689594894517444 8 0.4076511464588161 1.681612364699661 9 0.2798295866606221 1.2986238580539404 >10 1.3143430864038594 11.777462950558446 >50 0.07143890776482549 2.5221152084976177 >100 0.03499878022945805 3.1626480449134666 >500 0.0015012805447237388 0.54147951297848 >1k 0.002073196942679312 2.244288631543756 >5k 3.574477487378124E-4 1.1982293940463487 >10k+ 1.4297909949512498E-4 0.9264390441831358 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13512 0.49659819942725086 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11620 0.4270626907448679 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8488 0.311954227112086 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6931 0.2547307667429156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6428 0.23624431808158441 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5597 0.2057030877882122 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5061 0.18600381048707199 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4493 0.1651284569291473 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3812 0.14010008408945235 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3768 0.13848297923637368 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3564 0.13098549309937257 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3160 0.11613753035746838 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3097 0.11382213022692392 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.286333405858036E-4 0.0 0.0 3.675238302451531E-5 0.0 14-15 1.4700953209806125E-4 0.0 0.0 3.675238302451531E-5 0.0 16-17 1.653857236103189E-4 0.0 0.0 5.5128574536772966E-5 0.0 18-19 2.021381066348342E-4 0.0 0.0 1.4700953209806125E-4 0.0 20-21 2.2051429814709186E-4 0.0 0.0 2.2051429814709186E-4 0.0 22-23 2.2051429814709186E-4 0.0 0.0 3.491476387328954E-4 0.0 24-25 3.307714472206378E-4 0.0 0.0 5.512857453677297E-4 0.0 26-27 3.307714472206378E-4 0.0 0.0 8.636810010761097E-4 0.0 28-29 3.8590002175741076E-4 0.0 0.0 0.002921814450448967 0.0 30-31 4.042762132696684E-4 0.0 0.0 0.009592371969398496 0.0 32-33 4.4102859629418373E-4 0.0 0.0 0.023778791816861405 0.0 34-35 5.880381283922449E-4 0.0 0.0 0.04443363107663901 0.0 36-37 6.982952774657908E-4 0.0 0.0 0.07690436147879828 0.0 38-39 6.982952774657908E-4 0.0 0.0 0.12718162145633521 0.0 40-41 6.982952774657908E-4 0.0 0.0 0.18245720552520625 0.0 42-43 7.350476604903062E-4 0.0 0.0 0.23944177540471723 0.0 44-45 7.718000435148215E-4 0.0 0.0 0.30747043638309507 0.0 46-47 8.085524265393368E-4 0.0 0.0 0.38621241701311915 0.0 48-49 8.085524265393368E-4 0.0 0.0 0.46348430232216253 0.0 50-51 8.085524265393368E-4 0.0 0.0 0.5473532403841065 0.0 52-53 8.085524265393368E-4 0.0 0.0 0.6284657497192119 0.0 54-55 8.269286180515945E-4 0.0 0.0 0.7178107928518085 0.0 56-57 8.820571925883675E-4 0.0 0.0 0.811492617181298 3.675238302451531E-5 58-59 9.188095756128827E-4 0.0 0.0 0.8813588973109017 3.675238302451531E-5 60-61 9.188095756128827E-4 0.0 0.0 0.9419452007268152 3.675238302451531E-5 62-63 9.188095756128827E-4 1.8376191512257656E-5 0.0 0.9956036799426076 3.675238302451531E-5 64-65 9.188095756128827E-4 3.675238302451531E-5 0.0 1.0531579117589984 3.675238302451531E-5 66-67 9.55561958637398E-4 5.5128574536772966E-5 0.0 1.102277471671263 3.675238302451531E-5 68-69 9.55561958637398E-4 7.350476604903063E-5 0.0 1.165326184749819 3.675238302451531E-5 70-71 9.55561958637398E-4 7.350476604903063E-5 0.0 1.261102894911706 3.675238302451531E-5 72-73 9.55561958637398E-4 7.350476604903063E-5 0.0 1.383267816085195 3.675238302451531E-5 74-75 9.739381501496557E-4 7.350476604903063E-5 0.0 1.5514099684223526 3.675238302451531E-5 76-77 9.923143416619134E-4 7.350476604903063E-5 0.0 1.7626994184302909 3.675238302451531E-5 78-79 0.001010690533174171 7.350476604903063E-5 0.0 2.0446820771858847 3.675238302451531E-5 80-81 0.00117607625678449 7.350476604903063E-5 0.0 2.37795268645219 3.675238302451531E-5 82-83 0.00117607625678449 7.350476604903063E-5 0.0 2.698010814021181 3.675238302451531E-5 84-85 0.0011944524482967477 7.350476604903063E-5 0.0 2.9980388928418122 3.675238302451531E-5 86-87 0.0012128286398090053 1.1025714907354593E-4 0.0 3.276401441869491 3.675238302451531E-5 88-89 0.0012128286398090053 1.1025714907354593E-4 0.0 3.550059685870032 3.675238302451531E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11415 0.0 27.416168 1 TACATGG 11475 0.0 26.443785 2 ACATGGG 11550 0.0 25.492006 3 CATGGGA 8205 0.0 22.772882 4 AGTACTT 9770 0.0 19.715307 12-13 CATGGGG 3875 0.0 19.482723 4 ATGGGAG 3465 0.0 19.064535 5 GTGTAGC 1645 0.0 18.651623 1 GAGTACT 9535 0.0 18.44566 12-13 ATGGGAT 3485 0.0 18.142763 5 CGCATAG 660 0.0 18.031986 66-67 AGTACAT 8270 0.0 17.176168 2 GTAGCCC 1545 0.0 16.797152 3 GTACTTT 10340 0.0 16.735996 14-15 AGAGTAC 19325 0.0 16.055079 10-11 TACTTTT 11905 0.0 15.981597 14-15 TGCGCGA 125 7.202987E-5 15.544308 66-67 TAGCCCT 1880 0.0 15.058954 4 GTATCAA 29785 0.0 14.83363 1 ACTTTTT 12025 0.0 14.724172 16-17 >>END_MODULE