FastQCFastQC Report
Fri 27 May 2016
SRR939633_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939633_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052811
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61020.2972509402960136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41870.2039642227170451No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34550.16830580116727745No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32660.159098913635985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30160.14692049097554524No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24750.12056638433835361No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG79150.027.4612731
TACATGG78150.026.9624982
ACATGGG80500.025.5858573
CATGGGA49900.021.4938414
GAGTACT53100.021.09125512-13
AGTACTT55300.020.80997712-13
GTATCAA155250.019.71671
CATGGGG36400.019.6870984
GTACTTT60700.018.7245914-15
AGTACAT58300.018.4783612
TATCAAC173500.017.7521652
CAACGCA174150.017.6863385
TCAACGC174200.017.6535874
ATCAACG174700.017.5758953
AACGCAG176250.017.5551056
TAGGACG1901.8793554E-417.4843924
GTGTACG2202.941436E-517.2573241
ACGCAGA185700.016.3035857
ATGGGAT25850.016.1561875
CGCAGAG184300.016.04168
Produced by FastQC (version 0.11.5)