##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939633_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2052811 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.17323903661857 31.0 30.0 33.0 26.0 34.0 2 30.308930047627374 31.0 30.0 34.0 26.0 34.0 3 30.35245232025744 31.0 30.0 34.0 26.0 34.0 4 33.93610566194355 35.0 35.0 37.0 28.0 37.0 5 33.92849755773912 35.0 35.0 37.0 28.0 37.0 6 34.02898026169969 36.0 35.0 37.0 29.0 37.0 7 33.8351085414098 35.0 35.0 37.0 28.0 37.0 8 33.83346348007683 35.0 35.0 37.0 28.0 37.0 9 35.09632109336904 37.0 35.0 39.0 28.0 39.0 10-11 34.97245776644806 37.0 34.0 39.0 27.5 39.0 12-13 34.813300883520206 37.0 34.0 39.0 27.0 39.0 14-15 35.501604628969744 38.0 34.0 40.0 27.0 41.0 16-17 35.2423732628089 38.0 33.5 40.0 27.0 41.0 18-19 35.2052288301261 38.0 33.5 40.0 27.0 41.0 20-21 35.326416070451685 38.0 34.0 40.0 27.0 41.0 22-23 35.19054335737679 38.0 34.0 40.0 26.0 41.0 24-25 35.061807199980905 38.0 34.0 40.0 25.0 41.0 26-27 35.19403759040652 38.0 34.0 40.0 25.5 41.0 28-29 35.077557554007655 38.0 34.0 40.0 25.0 41.0 30-31 34.862028213995345 38.0 34.0 40.0 25.0 41.0 32-33 34.64727196025352 38.0 33.0 40.0 24.0 41.0 34-35 34.41646503258215 38.0 33.0 40.0 24.0 41.0 36-37 34.141174954732804 38.0 33.0 40.0 23.5 41.0 38-39 33.97640942103291 38.0 33.0 40.0 23.0 41.0 40-41 33.70143330291975 38.0 32.5 40.0 21.0 41.0 42-43 33.40101256277368 37.5 32.0 40.0 19.0 41.0 44-45 33.14994317547987 37.0 31.0 40.0 18.5 41.0 46-47 32.715378327571315 37.0 31.0 40.0 15.0 41.0 48-49 32.365600145361654 37.0 30.5 40.0 13.5 41.0 50-51 31.756940117721506 36.0 30.0 38.5 11.0 40.5 52-53 31.81541968549467 36.5 30.0 39.0 9.0 40.0 54-55 31.85660418811084 36.5 30.0 39.0 8.5 40.0 56-57 31.709015832436595 36.0 30.0 39.0 8.0 40.0 58-59 31.313866205900105 36.0 29.5 39.0 7.0 40.0 60-61 30.888988075375668 35.0 29.0 39.0 4.0 40.0 62-63 30.4694326462592 35.0 28.0 38.5 2.0 40.0 64-65 29.835415924797754 34.5 27.5 38.0 2.0 40.0 66-67 29.29310321310632 34.0 26.5 38.0 2.0 40.0 68-69 28.772288340232002 34.0 26.0 37.0 2.0 39.0 70-71 28.20708530887646 33.5 26.0 36.5 2.0 39.0 72-73 27.447740439816428 32.5 24.5 36.0 2.0 38.5 74-75 26.967363775817645 32.0 24.0 35.5 2.0 38.0 76-77 26.56105359918668 32.0 22.5 35.0 2.0 37.5 78-79 25.967388863368328 31.5 21.5 35.0 2.0 37.0 80-81 25.282462194522537 31.0 19.5 34.5 2.0 36.0 82-83 24.59280566988388 31.0 17.5 34.0 2.0 36.0 84-85 23.907014820166104 30.0 10.0 34.0 2.0 35.0 86-87 23.210871580481594 30.0 4.5 34.0 2.0 35.0 88-89 22.37095134427865 29.0 2.0 34.0 2.0 35.0 90-91 21.061581655593233 28.0 2.0 33.5 2.0 35.0 92-93 19.33287136516708 25.0 2.0 32.0 2.0 34.0 94-95 19.09159318612381 25.0 2.0 32.0 2.0 34.0 96-97 18.466926814012588 25.0 2.0 32.0 2.0 34.0 98-99 17.47467959787823 22.5 2.0 32.0 2.0 34.0 100-101 15.863388300238064 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0294894724971115 1101 2 0.9877296898069758 1101 3 1.1240395277173008 1101 4 1.2288416017131425 1101 5 0.9161212166569328 1101 6 0.900356040143059 1101 7 0.8723917631109686 1101 8 0.789577898145744 1101 9 1.166762240055668 1101 10-11 0.9570666657047795 1101 12-13 0.9898019856126936 1101 14-15 1.074082597502155 1101 16-17 0.9806452068351987 1101 18-19 0.9558432307302311 1101 20-21 1.119874989225373 1101 22-23 1.226925584897252 1101 24-25 1.0801513198869301 1101 26-27 1.0729866516762883 1101 28-29 1.158900360331387 1101 30-31 1.1710952160696664 1101 32-33 1.2107820624292458 1101 34-35 1.329907009005133 1101 36-37 1.1865434168403866 1101 38-39 1.1953127183443613 1101 40-41 1.1744288387787094 1101 42-43 1.1787631672487109 1101 44-45 1.1187825016874626 1101 46-47 1.0839621501689507 1101 48-49 1.067438178980268 1101 50-51 1.119682122497128 1101 52-53 1.2365192203928252 1101 54-55 1.2070685614054746 1101 56-57 0.9960134356194885 1101 58-59 0.8884337996534413 1101 60-61 0.5292011500019349 1101 62-63 0.46530552943921677 1101 64-65 0.5670790011036253 1101 66-67 0.5909626007227295 1101 68-69 0.49155393247854207 1101 70-71 0.4166598798860299 1101 72-73 0.5915006649261798 1101 74-75 0.3556449629070819 1101 76-77 -0.13344509094781287 1101 78-79 0.046479157941941907 1101 80-81 0.08030320860398632 1101 82-83 -0.004753929587064221 1101 84-85 -0.12924666528843787 1101 86-87 -0.5593684547772 1101 88-89 -0.8808923842168426 1101 90-91 -4.37187192382795 1101 92-93 -9.49566732588393 1101 94-95 -7.86441828413183 1101 96-97 -4.596616464766932 1101 98-99 -3.7525882309702165 1101 100-101 -3.179156449973826 1102 1 1.1039763493631902 1102 2 1.0557828863254421 1102 3 1.0264487012706205 1102 4 1.1780869224646295 1102 5 1.0402116585004606 1102 6 1.0604749302220284 1102 7 1.044322848263242 1102 8 0.9541392645838798 1102 9 1.198588892110827 1102 10-11 1.0684757147404653 1102 12-13 1.05955948348754 1102 14-15 1.2278129516119662 1102 16-17 1.2500978521340613 1102 18-19 1.2191439774586783 1102 20-21 1.395099916037573 1102 22-23 1.424608775394674 1102 24-25 1.3058075843904078 1102 26-27 1.1845707530929275 1102 28-29 1.2657944324836947 1102 30-31 1.4056461359638703 1102 32-33 1.1773434191646999 1102 34-35 1.1946964960517974 1102 36-37 1.1832462601007165 1102 38-39 1.1424454169432394 1102 40-41 1.0617973877817874 1102 42-43 1.123308756063473 1102 44-45 1.0839729516704537 1102 46-47 0.8937401799766391 1102 48-49 0.8771079970389906 1102 50-51 0.9446401180112609 1102 52-53 1.103871021505288 1102 54-55 1.0597156929990454 1102 56-57 1.1561575256947947 1102 58-59 1.1558060302816529 1102 60-61 0.9573025957540224 1102 62-63 0.8748849778854115 1102 64-65 0.8069428079255765 1102 66-67 1.1117868265707571 1102 68-69 0.7922729005023079 1102 70-71 0.8059837868934601 1102 72-73 0.827790115048785 1102 74-75 0.6355646355604812 1102 76-77 0.2776622588804223 1102 78-79 0.04786973955785356 1102 80-81 0.1055294830095761 1102 82-83 -0.039914797200029994 1102 84-85 -0.5279953709249945 1102 86-87 -0.8351977772234136 1102 88-89 -1.5695089664073727 1102 90-91 -5.402923844444995 1102 92-93 -10.566456541255768 1102 94-95 -9.148918830985227 1102 96-97 -5.6709619790867265 1102 98-99 -4.476476318190773 1102 100-101 -3.848900930096553 1103 1 0.8077603613369426 1103 2 0.766372050046531 1103 3 0.8870780730413479 1103 4 0.8745995192958915 1103 5 0.8519045475205047 1103 6 0.7548178804033014 1103 7 0.8526987386079696 1103 8 0.7436817750571549 1103 9 0.7368478548887154 1103 10-11 0.6672620183304261 1103 12-13 0.6762345093702251 1103 14-15 0.6811462496557255 1103 16-17 0.6921361730741395 1103 18-19 0.8414071061698749 1103 20-21 0.8078483380425681 1103 22-23 0.6124645107652711 1103 24-25 0.6229917504913374 1103 26-27 0.7300920880619373 1103 28-29 0.8157721780009268 1103 30-31 0.8080217413453497 1103 32-33 0.9138499020785957 1103 34-35 0.7264630608639493 1103 36-37 0.866874636264555 1103 38-39 0.8227825999271374 1103 40-41 0.6152161968559611 1103 42-43 0.6722193230388811 1103 44-45 0.5968354471986004 1103 46-47 0.6446952038654743 1103 48-49 0.6750774353923035 1103 50-51 0.6490742299647714 1103 52-53 0.6611931874553569 1103 54-55 0.6936348551894547 1103 56-57 0.6615132687849865 1103 58-59 0.37013467495498276 1103 60-61 0.38252312245962017 1103 62-63 0.6300896375197524 1103 64-65 0.6514236741432704 1103 66-67 0.5448943507915303 1103 68-69 0.4758115088317929 1103 70-71 0.5495407943205883 1103 72-73 0.4252218506911163 1103 74-75 0.26210927090677316 1103 76-77 0.06522381160369761 1103 78-79 0.02687862326506263 1103 80-81 -0.19346566864512837 1103 82-83 0.04579617066485042 1103 84-85 -0.1119082251054131 1103 86-87 -0.5507081812492487 1103 88-89 -1.0295724258700254 1103 90-91 -2.7439529499559434 1103 92-93 -9.449953662288834 1103 94-95 -6.125687930029365 1103 96-97 -3.6185269769539143 1103 98-99 -2.7376623387584154 1103 100-101 -2.441436679530046 1104 1 0.9073151349006352 1104 2 0.8989141296496364 1104 3 0.9143656352255043 1104 4 0.8037287708122989 1104 5 0.9574096279366628 1104 6 0.7319339475041176 1104 7 0.9492765118437347 1104 8 0.8741926732136633 1104 9 0.9239685474522332 1104 10-11 0.8494338351850885 1104 12-13 0.8418896895395918 1104 14-15 0.8621366650567523 1104 16-17 1.0626272190894923 1104 18-19 0.9584858986901637 1104 20-21 0.8788396481648562 1104 22-23 0.9831515335720482 1104 24-25 0.9346147543497167 1104 26-27 0.8435033442300721 1104 28-29 0.7465624097788819 1104 30-31 0.9836618877348826 1104 32-33 0.922173682825921 1104 34-35 0.9467664206243924 1104 36-37 0.8745249135255477 1104 38-39 0.8126958489022087 1104 40-41 0.9041726891874262 1104 42-43 0.8010978679101797 1104 44-45 0.8446189599481784 1104 46-47 0.6577028299277998 1104 48-49 0.7774973691254701 1104 50-51 0.6597256198814172 1104 52-53 0.7196250529943491 1104 54-55 0.7982001704576298 1104 56-57 0.7470403109144499 1104 58-59 0.4172966437193608 1104 60-61 0.5694267014678154 1104 62-63 0.3644031831482799 1104 64-65 0.5697832314286195 1104 66-67 0.5897783715211524 1104 68-69 0.49247548723043266 1104 70-71 0.49185661615285525 1104 72-73 0.7333177853321367 1104 74-75 0.5372684840938149 1104 76-77 0.4258581484613302 1104 78-79 0.3244655846315716 1104 80-81 0.1646583183805923 1104 82-83 0.3309808793037945 1104 84-85 -0.046500201425676124 1104 86-87 -0.12925578185716802 1104 88-89 -0.09475615359895428 1104 90-91 -0.4111704037515942 1104 92-93 -4.097095397219039 1104 94-95 -1.4275116838940072 1104 96-97 -0.682364313344074 1104 98-99 -0.5322253064093161 1104 100-101 -0.26174781679875814 1105 1 0.7868148483326962 1105 2 0.8070076828322321 1105 3 0.7898191741441316 1105 4 0.8482375354816085 1105 5 0.6615261716209986 1105 6 0.6068496301959811 1105 7 0.8165401017446285 1105 8 0.770512540917089 1105 9 1.0234227268972091 1105 10-11 0.7573758126242822 1105 12-13 0.7556905183789979 1105 14-15 0.9720637249077271 1105 16-17 0.9045552667706787 1105 18-19 0.6769926315051222 1105 20-21 0.6631529635710578 1105 22-23 0.7460097514173398 1105 24-25 0.9110971704015824 1105 26-27 0.8501913730419304 1105 28-29 0.8876900952838298 1105 30-31 1.0653181173289354 1105 32-33 0.9965141382122695 1105 34-35 0.8432987056239725 1105 36-37 0.8768795661192286 1105 38-39 0.8382224716235314 1105 40-41 0.8205787195148275 1105 42-43 1.0822402398899342 1105 44-45 0.9889375525140593 1105 46-47 0.766608153077776 1105 48-49 0.7704370026860943 1105 50-51 0.7799125090760661 1105 52-53 0.7209062113161089 1105 54-55 0.9231434747919387 1105 56-57 0.8229058189720462 1105 58-59 0.7250679690532991 1105 60-61 0.4638733676147986 1105 62-63 0.43678519542771 1105 64-65 0.479199137734323 1105 66-67 0.5956323640473613 1105 68-69 0.5658163877318891 1105 70-71 0.4561356532592349 1105 72-73 0.6799956569847687 1105 74-75 0.4576372430550073 1105 76-77 0.3548970399451399 1105 78-79 0.17608698032411496 1105 80-81 0.06860292130178891 1105 82-83 0.29985852377237876 1105 84-85 0.48702835301494574 1105 86-87 -0.07270112913673543 1105 88-89 -0.5005932879449198 1105 90-91 -1.3428398473703815 1105 92-93 -7.388057553561971 1105 94-95 -3.0013747020955606 1105 96-97 -1.4157500802655782 1105 98-99 -0.7215046445546243 1105 100-101 -0.6322242843213051 1106 1 0.6153130690620401 1106 2 0.5860043822111081 1106 3 0.659095066558006 1106 4 0.6610436068961008 1106 5 0.5892806779299136 1106 6 0.5313409338533504 1106 7 0.5635636070047099 1106 8 0.6249878682229308 1106 9 0.6879456268912136 1106 10-11 0.36386811811671294 1106 12-13 0.546225582494877 1106 14-15 0.5541474928410395 1106 16-17 0.6466877544465248 1106 18-19 0.7179144088558544 1106 20-21 0.6104085079084953 1106 22-23 0.7616684913814495 1106 24-25 0.6540006345280673 1106 26-27 0.5568343324333114 1106 28-29 0.6373367913997185 1106 30-31 0.735812493310199 1106 32-33 0.5755583234149952 1106 34-35 0.7413520763936035 1106 36-37 0.7133038212187728 1106 38-39 0.6258344402784317 1106 40-41 0.7189039310349941 1106 42-43 0.6362088141637159 1106 44-45 0.4231152292322591 1106 46-47 0.5157184063852469 1106 48-49 0.6215888791445536 1106 50-51 0.6283341733918313 1106 52-53 0.5681011956371016 1106 54-55 0.6722388052560682 1106 56-57 0.5710395169573133 1106 58-59 0.5475762272879763 1106 60-61 0.4866153353943723 1106 62-63 0.6303410439461494 1106 64-65 0.5611010474350842 1106 66-67 0.4602731545036889 1106 68-69 0.4477301466157151 1106 70-71 0.4989744591555798 1106 72-73 0.635166878793683 1106 74-75 0.6816373624377512 1106 76-77 0.5467678321766201 1106 78-79 0.5299461278377571 1106 80-81 0.6183961062842407 1106 82-83 0.4726566972163546 1106 84-85 0.17796836073473443 1106 86-87 0.09917104846915592 1106 88-89 -0.05761982009217803 1106 90-91 -0.6474403595134568 1106 92-93 -4.065838286211214 1106 94-95 -1.6054226681231505 1106 96-97 -0.751858060178094 1106 98-99 -0.5339121424147528 1106 100-101 -0.2722045638952064 1107 1 0.431465579049501 1107 2 0.46954227811314553 1107 3 0.47603594693580575 1107 4 0.4969677672518742 1107 5 0.24003325639271367 1107 6 0.385179576745152 1107 7 0.32058855817799525 1107 8 0.44476101772956866 1107 9 0.48241927391336503 1107 10-11 0.26631147108067665 1107 12-13 0.33548773967997647 1107 14-15 0.3141106449028257 1107 16-17 0.4999371036992315 1107 18-19 0.37681116650625057 1107 20-21 0.4328849565556183 1107 22-23 0.5219110784180003 1107 24-25 0.59545862150717 1107 26-27 0.2899489091505032 1107 28-29 0.3812557462181161 1107 30-31 0.4164161125387622 1107 32-33 0.2873620976721014 1107 34-35 0.16768199396965144 1107 36-37 0.14256212914575173 1107 38-39 0.04531009901193528 1107 40-41 -0.11676350188242424 1107 42-43 -0.14455477194271538 1107 44-45 -0.144073284546252 1107 46-47 0.09048120632589729 1107 48-49 0.025278611303647835 1107 50-51 0.1007198249625425 1107 52-53 0.34737809949317366 1107 54-55 0.46097637425777194 1107 56-57 0.06445697693638053 1107 58-59 0.06770116723977182 1107 60-61 -0.015790891849864153 1107 62-63 -0.14728493382824226 1107 64-65 -0.09000570245727957 1107 66-67 -0.1454410096418144 1107 68-69 -0.21193712404202714 1107 70-71 -0.2512938976278747 1107 72-73 0.06177612111373776 1107 74-75 -0.09786804978229569 1107 76-77 -0.19494342099499207 1107 78-79 0.007108408900180052 1107 80-81 -0.12376577023129798 1107 82-83 0.05721074434970319 1107 84-85 -0.19098214763850407 1107 86-87 -0.3418671797786246 1107 88-89 -0.5633608584967966 1107 90-91 -0.9367449555408704 1107 92-93 -4.25275493125428 1107 94-95 -1.9600081244090468 1107 96-97 -1.3169556066839014 1107 98-99 -1.1877661735205542 1107 100-101 -1.29164111275532 1108 1 0.4055804003479473 1108 2 0.3565085553789089 1108 3 0.10730998148954285 1108 4 -0.23575055491298968 1108 5 -0.8849315195292959 1108 6 -0.18341261647876195 1108 7 0.15858993183775993 1108 8 0.3029795852210171 1108 9 0.20899058488748778 1108 10-11 0.1686781486061122 1108 12-13 0.22689135233328983 1108 14-15 0.34072873810325177 1108 16-17 0.415955397177477 1108 18-19 0.28337978850650103 1108 20-21 0.3102010519879812 1108 22-23 0.30741467650604193 1108 24-25 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68-69 0.3814285875508894 2311 70-71 0.6294995890776498 2311 72-73 0.4554362512777388 2311 74-75 0.6807539142251926 2311 76-77 0.6392295111762287 2311 78-79 0.6380621594998424 2311 80-81 0.6214165485219176 2311 82-83 0.9459211612905527 2311 84-85 0.8520844754420445 2311 86-87 0.9592237821868252 2311 88-89 0.9596492158401766 2311 90-91 1.6301775319245806 2311 92-93 2.6391515298217563 2311 94-95 1.9448955262114183 2311 96-97 1.665896899588681 2311 98-99 1.4468494478575913 2311 100-101 1.452461069805418 2312 1 0.3317835595970706 2312 2 0.2156237728339896 2312 3 0.05919974724911725 2312 4 0.11022976369422821 2312 5 0.17990203104257319 2312 6 0.16962718637202556 2312 7 0.31854588340544154 2312 8 -0.6171628282642629 2312 9 0.06657437539968214 2312 10-11 0.02699024704660502 2312 12-13 0.2915144050447154 2312 14-15 -0.030809520328190843 2312 16-17 0.10759793010902996 2312 18-19 0.12090676869518546 2312 20-21 0.31830359556492027 2312 22-23 0.13349168372729991 2312 24-25 0.36485256926326315 2312 26-27 0.286303913841671 2312 28-29 0.13587421201503957 2312 30-31 0.42573582945541233 2312 32-33 0.3584182261209605 2312 34-35 0.18675541393746897 2312 36-37 0.35615898160532566 2312 38-39 0.3928027650814201 2312 40-41 0.5521788413242987 2312 42-43 0.47225765349742943 2312 44-45 0.30018873736850793 2312 46-47 0.3742494456306744 2312 48-49 0.22447623048267218 2312 50-51 0.18387099520737138 2312 52-53 0.130863170539957 2312 54-55 0.22927426556701036 2312 56-57 0.11653955847992137 2312 58-59 0.12530032919757872 2312 60-61 0.13035099370260994 2312 62-63 0.1807447967161444 2312 64-65 0.10180927022356556 2312 66-67 0.19619862797074106 2312 68-69 0.159410238927034 2312 70-71 0.01160968082076863 2312 72-73 0.04373900357131433 2312 74-75 0.3704328133077617 2312 76-77 0.6497799698918278 2312 78-79 0.7965483980319519 2312 80-81 0.8404532457696199 2312 82-83 0.928031253033673 2312 84-85 0.8690569525062628 2312 86-87 0.6046366262235239 2312 88-89 1.049098757124586 2312 90-91 1.5856821190805448 2312 92-93 2.6304359334914835 2312 94-95 1.8481065353857282 2312 96-97 1.5762180005061097 2312 98-99 1.5778127506098834 2312 100-101 1.6396170331081752 2313 1 -0.19671409338650392 2313 2 -1.0269717964778096 2313 3 -0.5340306707677627 2313 4 -0.22932024186336974 2313 5 -0.81474942205557 2313 6 -0.45580823006307725 2313 7 -1.0876422883157844 2313 8 -1.1316869696153518 2313 9 -1.1777286590488814 2313 10-11 -1.9772431464227864 2313 12-13 -0.4938415377376728 2313 14-15 -0.048574168874239376 2313 16-17 -1.7410254955944353 2313 18-19 -1.1054428726259857 2313 20-21 -1.627096636999191 2313 22-23 -1.1444617521508391 2313 24-25 -0.3932886706796239 2313 26-27 -0.7997236432947119 2313 28-29 -2.0352602647584774 2313 30-31 -0.5280890805542455 2313 32-33 -0.15513889316218865 2313 34-35 -0.004908402678893253 2313 36-37 -0.1434440776386694 2313 38-39 -0.4941270467297656 2313 40-41 -0.26152277550924197 2313 42-43 -0.22128912010312973 2313 44-45 -0.7815351013072416 2313 46-47 -0.6419272261375895 2313 48-49 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-3.816015993760235 2314 8 -1.412269575295717 2314 9 -1.7106545008975758 2314 10-11 -1.0699128852887512 2314 12-13 -2.1492513103710493 2314 14-15 -2.975757630007216 2314 16-17 -2.361070547918196 2314 18-19 -2.5533907558742612 2314 20-21 -2.2466824753017036 2314 22-23 -3.3591409224823394 2314 24-25 -1.8781276204137 2314 26-27 -2.0391453437011293 2314 28-29 -1.2602709863031407 2314 30-31 -1.3261493325782467 2314 32-33 -1.9888763406571925 2314 34-35 -0.7003977549403757 2314 36-37 -0.48882717524359265 2314 38-39 -0.5167038684533978 2314 40-41 -0.5863399415101966 2314 42-43 -0.673659476757166 2314 44-45 -1.402267992036581 2314 46-47 -1.7747237106937561 2314 48-49 -2.005976552334623 2314 50-51 -1.036785178752023 2314 52-53 -0.5742400942081538 2314 54-55 -0.3636799144627396 2314 56-57 -0.4846250718391545 2314 58-59 -0.6250992735641709 2314 60-61 -0.4162967711873726 2314 62-63 -0.48895439419036535 2314 64-65 -0.5618088221730169 2314 66-67 -0.4578246990285635 2314 68-69 -0.3511741827989745 2314 70-71 -0.21736958024730413 2314 72-73 -0.007597806313210498 2314 74-75 0.14116443673965762 2314 76-77 0.13774244721399143 2314 78-79 0.017098598800636466 2314 80-81 0.13549701076278708 2314 82-83 0.3576844004159412 2314 84-85 0.051214334517045046 2314 86-87 0.289163895511507 2314 88-89 0.6385117559294287 2314 90-91 1.2976910526420156 2314 92-93 2.0754990445032355 2314 94-95 1.3733384726530424 2314 96-97 1.3572120419323852 2314 98-99 1.4203215423005524 2314 100-101 1.519379592973353 2315 1 -7.205375925514481 2315 2 -2.937878666018605 2315 3 -1.3398766583928214 2315 4 -0.8985307356982588 2315 5 -1.209339723522568 2315 6 -1.6080868075352512 2315 7 -2.720706436586106 2315 8 -2.638137841030815 2315 9 -3.2163703850292755 2315 10-11 -2.1155967856533877 2315 12-13 -2.584090905842956 2315 14-15 -4.245382765146726 2315 16-17 -3.9722683292017535 2315 18-19 -2.877959208870898 2315 20-21 -3.947200727455389 2315 22-23 -5.3023620907712825 2315 24-25 -3.3390289042615393 2315 26-27 -3.7607923548019038 2315 28-29 -2.532529880326784 2315 30-31 -2.327343552114961 2315 32-33 -1.3098947394181408 2315 34-35 -2.3211594111166747 2315 36-37 -2.758245216390762 2315 38-39 -1.9326704779762167 2315 40-41 -3.149914874007898 2315 42-43 -7.067706952324123 2315 44-45 -3.7620984133120814 2315 46-47 -2.331867936256664 2315 48-49 -5.110336409289111 2315 50-51 -6.231441389233275 2315 52-53 -6.612474007434649 2315 54-55 -4.205643588242705 2315 56-57 -3.5292919405779912 2315 58-59 -3.9084857033087133 2315 60-61 -2.8104135029428683 2315 62-63 -2.8220013336964307 2315 64-65 -1.624265761912941 2315 66-67 -2.257943019179237 2315 68-69 -1.747492946549027 2315 70-71 -1.28660035985612 2315 72-73 -1.733665071161468 2315 74-75 -2.161548292188023 2315 76-77 -2.2880909205975293 2315 78-79 -1.3172399236500638 2315 80-81 -1.151995996161034 2315 82-83 -1.7546498119613076 2315 84-85 -1.663306709470529 2315 86-87 -2.1226437449751216 2315 88-89 -1.6846855144773123 2315 90-91 -0.8256506214149759 2315 92-93 -0.02144163082555295 2315 94-95 -0.03087707059430045 2315 96-97 0.15407374721762324 2315 98-99 -0.10479259549587638 2315 100-101 -0.009983681819013768 2316 1 -4.303873186878651 2316 2 -2.9838522471555677 2316 3 -4.724925150192519 2316 4 -4.274797647359701 2316 5 -4.104810246126068 2316 6 -4.090282748736502 2316 7 -3.074270369298368 2316 8 -1.5274298529528565 2316 9 -1.9850180763046552 2316 10-11 -2.9738465359771844 2316 12-13 -4.091305670289064 2316 14-15 -2.9563693957663943 2316 16-17 -2.9598327963771354 2316 18-19 -3.205540236600555 2316 20-21 -5.286561400332751 2316 22-23 -6.088284254215566 2316 24-25 -4.489835280367526 2316 26-27 -4.062249547036977 2316 28-29 -4.542957275267721 2316 30-31 -6.73366084765356 2316 32-33 -4.702853870544956 2316 34-35 -6.626310055593372 2316 36-37 -9.598324683644172 2316 38-39 -6.37530298191642 2316 40-41 -5.523101795656384 2316 42-43 -4.843104191061386 2316 44-45 -3.7034791227045893 2316 46-47 -5.765106860776427 2316 48-49 -3.528513812032429 2316 50-51 -4.541878147031756 2316 52-53 -5.6930184771067935 2316 54-55 -7.026538462858436 2316 56-57 -5.156090877113424 2316 58-59 -6.011052881811594 2316 60-61 -6.495619115195726 2316 62-63 -6.613553890536782 2316 64-65 -5.338674712024517 2316 66-67 -6.6189236029534015 2316 68-69 -5.4541731503472235 2316 70-71 -6.278974995094796 2316 72-73 -7.950541036706149 2316 74-75 -8.205508858953 2316 76-77 -7.448685384027765 2316 78-79 -6.265436013096561 2316 80-81 -6.3940912086198765 2316 82-83 -5.242573188675884 2316 84-85 -6.830312779723169 2316 86-87 -5.365067632745671 2316 88-89 -5.393999997257456 2316 90-91 -5.609588541768266 2316 92-93 -4.4969709248660905 2316 94-95 -4.31909996122052 2316 96-97 -5.2183212327302595 2316 98-99 -5.833510625238555 2316 100-101 -5.078883097420656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10862.0 3 4287.0 4 4467.0 5 5043.0 6 5892.0 7 6829.0 8 8249.0 9 10115.0 10 12332.0 11 14365.0 12 16980.0 13 19518.0 14 20802.0 15 23146.0 16 25901.0 17 28786.0 18 26819.0 19 26580.0 20 28529.0 21 30874.0 22 34525.0 23 38448.0 24 43331.0 25 48625.0 26 54713.0 27 61832.0 28 70474.0 29 79908.0 30 91401.0 31 104102.0 32 119635.0 33 138869.0 34 162403.0 35 195540.0 36 224465.0 37 194059.0 38 59230.0 39 875.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.28684748496282 19.14442272692036 13.397605070101989 23.171124718014823 2 15.037781692664689 21.037651991041812 40.43106773204035 23.49349858425315 3 19.198509944269183 25.638466451804792 30.19703256571409 24.965991038211932 4 12.281147436669466 16.65680904710057 38.53531213871987 32.526731377510096 5 13.224121302782049 38.23209328042736 34.74363387607792 13.80015154071267 6 30.781534657495712 37.00819837962929 18.294569335222903 13.91569762765209 7 27.589483656156922 33.378611683862204 21.734632136154893 17.297272523825985 8 25.693443966490225 34.837402119737526 20.60432850032397 18.864825413448276 9 26.77709464496134 16.56453801267666 20.760190154794504 35.89817718756749 10-11 24.545561364512857 26.542541443467655 28.417925088991137 20.493972103028355 12-13 26.025163537151226 24.211933031798633 28.23838175619179 21.524521674858356 14-15 22.9215845980081 25.31095872143496 26.884396077828114 24.883060602728825 16-17 22.149017007495097 28.505868457331335 27.292367287784174 22.05274724738939 18-19 21.87230917904546 27.668855566726506 29.215184738419293 21.24365051580874 20-21 23.062427526938766 26.70629258782833 28.78476390726959 21.446515977963312 22-23 22.553218051430402 26.744852046709333 28.496683769820148 22.205246132040116 24-25 22.527308374340503 26.749951649867487 28.816118044074347 21.906621931717662 26-27 22.453755024003268 26.75573350386713 28.99957488499578 21.790936587133817 28-29 22.094104333277237 27.133261628289834 28.984398055671406 21.788235982761524 30-31 22.247899415860783 26.997426860648037 28.79045377495396 21.964219948537213 32-33 22.12651226088261 27.043620670522543 28.6955991546826 22.13426791391225 34-35 22.698203621729203 26.88453393886524 28.508027354291038 21.909235085114513 36-37 22.60610976862524 26.6514608598435 28.670246339923928 22.07218303160733 38-39 22.48805764715408 26.934383148332948 28.881008610590044 21.696550593922932 40-41 22.399825396302838 26.794034698826913 28.911958036586306 21.894181868283948 42-43 22.126289904240554 27.098523858899913 28.473047963611997 22.30213827324754 44-45 22.402462418395082 27.115156294463144 28.199109048529277 22.283272238612497 46-47 22.392439787450115 27.174982952500155 27.864589684482237 22.567987575567493 48-49 22.60875525576106 27.187161319452457 27.902215585177785 22.3018678396087 50-51 22.394368224388895 27.355227364375562 28.00572506281151 22.244679348424036 52-53 22.59999521816628 27.485321478289254 27.701699453943867 22.212983849600594 54-55 22.145702259793417 27.140892553628287 28.004590384539853 22.708814802038447 56-57 22.417783639796713 27.60465419155762 27.644164285898942 22.333397882746723 58-59 22.6199101720836 27.208172062625422 27.689941755982854 22.481976009308116 60-61 22.510569570415882 27.109774532901437 28.086757170200993 22.292898726481685 62-63 22.550669208601022 27.084330086660845 27.887292620440725 22.47770808429741 64-65 22.27613195815961 27.23975324896984 27.928632384853586 22.55548240801697 66-67 22.154842875633896 27.479279794226922 27.61356974737785 22.752307582761336 68-69 22.13624880513451 27.363931643939836 27.82027272195236 22.679546828973294 70-71 22.16228185585519 27.23412941217498 27.678434905306766 22.92515382666306 72-73 22.29769319824503 26.950058081075422 27.763656614729516 22.98859210595003 74-75 22.103701267667038 27.458027634902333 27.464614692505172 22.97365640492546 76-77 22.32474778021459 26.945326904313973 27.67219027854394 23.0577350369275 78-79 22.09415374901588 27.18297258571858 27.669304236384406 23.053569428881133 80-81 22.08102419912467 27.379618004098006 27.535037761877412 23.00432003489991 82-83 22.212704141284213 27.227060150801535 27.47453296612138 23.085702741792872 84-85 22.093471120646022 26.97046386741976 27.410755557615403 23.525309454318823 86-87 22.0703683854769 26.887122107836525 27.755060821084026 23.28744868560255 88-89 22.266380975338873 26.711342127889647 27.552313035976223 23.469963860795257 90-91 21.716382463016892 26.851780622866944 28.08471771953282 23.34711919458334 92-93 21.505310774145343 26.920069365413248 28.315483812981796 23.259136047459616 94-95 21.88594699914071 26.809720874242387 27.560222371536913 23.744109755079993 96-97 21.882904342419714 26.6016335864675 27.494166982657358 24.02129508845543 98-99 21.783732664628825 26.651096101867616 27.289635690272668 24.27553554323089 100-101 21.84804667526594 26.514987509899946 27.579922606531948 24.057043208302165 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1765.0 1 1084.5 2 388.0 3 373.0 4 362.0 5 373.0 6 509.5 7 881.5 8 1350.0 9 1600.0 10 1503.5 11 1346.5 12 1388.5 13 1504.0 14 1624.5 15 1767.0 16 1963.0 17 2180.5 18 2343.5 19 2555.5 20 2946.5 21 3418.0 22 3990.0 23 4818.5 24 5805.0 25 7374.0 26 9935.5 27 12953.5 28 15742.5 29 19125.0 30 23405.0 31 27847.0 32 32198.0 33 36506.0 34 41898.5 35 47002.0 36 51493.5 37 56032.5 38 58925.5 39 59622.0 40 60091.5 41 62607.5 42 65956.0 43 69175.0 44 72283.5 45 74527.0 46 76314.5 47 81139.0 48 92197.0 49 94138.5 50 84912.5 51 78434.5 52 73804.0 53 70992.5 54 67692.0 55 61340.0 56 55051.0 57 49287.0 58 43443.0 59 37644.0 60 31489.5 61 25302.0 62 19926.0 63 15674.5 64 11904.0 65 8785.5 66 6260.5 67 4560.0 68 3277.0 69 2198.5 70 1589.5 71 1174.0 72 829.0 73 567.5 74 402.0 75 280.5 76 178.0 77 108.0 78 74.5 79 49.0 80 33.0 81 21.5 82 13.0 83 13.5 84 11.0 85 7.0 86 5.5 87 4.5 88 4.5 89 3.0 90 2.0 91 1.5 92 1.5 93 2.5 94 2.0 95 2.0 96 2.0 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08417725742895961 2 0.09499169675143011 3 0.09163045209714873 4 0.09469941460757957 5 0.0914355973345817 6 0.07560364787601002 7 0.0873923610113157 8 0.08320298361612442 9 0.07989045265248482 10-11 0.08593095029206294 12-13 0.0851515312417948 14-15 0.09038825298578389 16-17 0.08785514107241242 18-19 0.09046132352174653 20-21 0.09981435212496426 22-23 0.10787646792617536 24-25 0.12965148764304166 26-27 0.1353022757574857 28-29 0.1337677945022703 30-31 0.13571634212794065 32-33 0.13121032574357794 34-35 0.1267773798951779 36-37 0.13235509747365928 38-39 0.12397634268327673 40-41 0.11951903998955579 42-43 0.120079247431936 44-45 0.11235812746521721 46-47 0.12943227603515375 48-49 0.1479191216337013 50-51 0.15717472285563552 52-53 0.1650419838942796 54-55 0.16121795917890153 56-57 0.1316243921140329 58-59 0.12458526381629872 60-61 0.1202010316585404 62-63 0.11710771230278871 64-65 0.10527028547684127 66-67 0.1167667164682964 68-69 0.11550016051161066 70-71 0.12336742155025476 72-73 0.1505253040830354 74-75 0.16258194251687078 76-77 0.16842758539388186 78-79 0.16496891335831695 80-81 0.17156961844027532 82-83 0.17025434879294782 84-85 0.14777298056177604 86-87 0.14952667342487935 88-89 0.1711311952244995 90-91 0.2641987011955801 92-93 0.54362043071671 94-95 0.16796480533278513 96-97 0.18024065537450842 98-99 0.19843521882920542 100-101 0.2040372932530077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2052811.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.43462692995295 #Duplication Level Percentage of deduplicated Percentage of total 1 77.02397966804575 43.46819557226443 2 12.52784374829404 14.14008376343435 3 4.403788908138282 7.455785523271465 4 2.059188798473498 4.6483820648075955 5 1.137930972456323 3.210935495130557 6 0.6768435786320401 2.2918448910020075 7 0.43539489392287095 1.719994388402056 8 0.3126465941832481 1.4115275122921602 9 0.2211253252371247 1.1231212711069476 >10 1.0845908974204015 10.855314672894059 >50 0.07126857943673663 2.7464377557491684 >100 0.04162361710408407 4.32992828699754 >500 0.0024577609850856366 0.9219279509308013 >1k 0.0012288804925428094 1.3742477682404033 >5k 8.77771780387721E-5 0.30227308347642357 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6102 0.2972509402960136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4187 0.2039642227170451 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3455 0.16830580116727745 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3266 0.159098913635985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3016 0.14692049097554524 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2475 0.12056638433835361 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.871369064175903E-5 0.0 8 0.0 0.0 0.0 4.871369064175903E-5 0.0 9 0.0 0.0 0.0 4.871369064175903E-5 0.0 10-11 0.0 0.0 0.0 4.871369064175903E-5 0.0 12-13 0.0 0.0 0.0 4.871369064175903E-5 0.0 14-15 0.0 0.0 0.0 4.871369064175903E-5 0.0 16-17 0.0 0.0 0.0 4.871369064175903E-5 0.0 18-19 0.0 0.0 0.0 4.871369064175903E-5 0.0 20-21 0.0 0.0 0.0 9.742738128351806E-5 0.0 22-23 0.0 0.0 0.0 3.8970952513407226E-4 4.871369064175903E-5 24-25 0.0 0.0 0.0 7.063485143055059E-4 9.742738128351806E-5 26-27 2.4356845320879516E-5 0.0 0.0 0.0010717011941186988 9.742738128351806E-5 28-29 4.871369064175903E-5 0.0 0.0 0.0032881741183187346 9.742738128351806E-5 30-31 4.871369064175903E-5 0.0 0.0 0.010643941405224348 9.742738128351806E-5 32-33 4.871369064175903E-5 0.0 0.0 0.025623401277565253 9.742738128351806E-5 34-35 4.871369064175903E-5 0.0 0.0 0.04893290224964694 9.742738128351806E-5 36-37 4.871369064175903E-5 0.0 0.0 0.08205821188604309 9.742738128351806E-5 38-39 4.871369064175903E-5 0.0 0.0 0.13033347931202627 9.742738128351806E-5 40-41 4.871369064175903E-5 0.0 0.0 0.18625679616876567 9.742738128351806E-5 42-43 4.871369064175903E-5 0.0 0.0 0.24610156512216663 1.2178422660439757E-4 44-45 4.871369064175903E-5 0.0 0.0 0.3171017692325304 1.704979172461566E-4 46-47 4.871369064175903E-5 0.0 0.0 0.4020340888664373 1.9485476256703613E-4 48-49 4.871369064175903E-5 0.0 0.0 0.4944196031685333 2.4356845320879517E-4 50-51 4.871369064175903E-5 0.0 0.0 0.5946236648186316 2.4356845320879517E-4 52-53 4.871369064175903E-5 0.0 0.0 0.6949738675406553 2.679252985296747E-4 54-55 4.871369064175903E-5 0.0 0.0 0.8022414143338086 2.922821438505542E-4 56-57 4.871369064175903E-5 0.0 0.0 0.9322095409660217 2.922821438505542E-4 58-59 4.871369064175903E-5 0.0 0.0 1.0750624387729801 2.922821438505542E-4 60-61 4.871369064175903E-5 0.0 0.0 1.238204588732231 2.922821438505542E-4 62-63 4.871369064175903E-5 0.0 0.0 1.403806780068891 2.922821438505542E-4 64-65 4.871369064175903E-5 0.0 0.0 1.5744995520776146 2.922821438505542E-4 66-67 4.871369064175903E-5 0.0 0.0 1.763192032778468 2.922821438505542E-4 68-69 4.871369064175903E-5 0.0 0.0 1.9820139311412497 2.922821438505542E-4 70-71 4.871369064175903E-5 0.0 0.0 2.2244376126199636 2.922821438505542E-4 72-73 4.871369064175903E-5 0.0 0.0 2.4770668122881263 3.409958344923132E-4 74-75 4.871369064175903E-5 0.0 0.0 2.738123480437313 3.409958344923132E-4 76-77 4.871369064175903E-5 0.0 0.0 3.021369234673821 3.409958344923132E-4 78-79 4.871369064175903E-5 0.0 0.0 3.340078555697529 3.409958344923132E-4 80-81 4.871369064175903E-5 0.0 0.0 3.686603394077682 3.653526798131927E-4 82-83 4.871369064175903E-5 0.0 0.0 4.028232506548338 4.384232157758313E-4 84-85 4.871369064175903E-5 0.0 0.0 4.3819182574528295 4.384232157758313E-4 86-87 4.871369064175903E-5 0.0 0.0 4.762055542375796 4.384232157758313E-4 88-89 4.871369064175903E-5 0.0 0.0 5.1641139880875535 4.384232157758313E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7915 0.0 27.461273 1 TACATGG 7815 0.0 26.962498 2 ACATGGG 8050 0.0 25.585857 3 CATGGGA 4990 0.0 21.493841 4 GAGTACT 5310 0.0 21.091255 12-13 AGTACTT 5530 0.0 20.809977 12-13 GTATCAA 15525 0.0 19.7167 1 CATGGGG 3640 0.0 19.687098 4 GTACTTT 6070 0.0 18.72459 14-15 AGTACAT 5830 0.0 18.478361 2 TATCAAC 17350 0.0 17.752165 2 CAACGCA 17415 0.0 17.686338 5 TCAACGC 17420 0.0 17.653587 4 ATCAACG 17470 0.0 17.575895 3 AACGCAG 17625 0.0 17.555105 6 TAGGACG 190 1.8793554E-4 17.484392 4 GTGTACG 220 2.941436E-5 17.257324 1 ACGCAGA 18570 0.0 16.303585 7 ATGGGAT 2585 0.0 16.156187 5 CGCAGAG 18430 0.0 16.0416 8 >>END_MODULE