FastQCFastQC Report
Fri 27 May 2016
SRR939633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939633_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052811
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63590.30977035879094567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48050.23406928353365214No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38210.18613501194216125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35010.1705466309367984No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32580.15870920411085093No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27900.13591119689050768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG82100.026.1879861
TACATGG81650.025.7517192
ACATGGG82250.023.9024453
CATGGGG36650.019.7628084
GTATCAA139350.019.682971
AGTACTT54150.019.51515412-13
GAGTACT51850.019.42971812-13
CATGGGA52300.019.1548964
TCAACGC154400.017.6982674
GTACTTT57950.017.62740914-15
ATCAACG154450.017.6011833
CAACGCA157500.017.2603365
AACGCAG157850.017.2274886
AGTACAT60050.016.9981482
GCACCGT4154.7293724E-1116.9758346
TATCAAC162800.016.7871362
TCGCGCC1205.0150622E-516.21658370-71
GGTATCA108300.016.160081
CGCGTAA900.002300053816.14549894-95
ACGCAGA169850.015.8720957
Produced by FastQC (version 0.11.5)