##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939633_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2052811 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.542602801719205 31.0 31.0 34.0 30.0 34.0 2 31.75339181249516 31.0 31.0 34.0 30.0 34.0 3 31.801443971218003 31.0 31.0 34.0 30.0 34.0 4 35.58422816323568 37.0 35.0 37.0 33.0 37.0 5 35.317975205705736 37.0 35.0 37.0 33.0 37.0 6 35.33893427110436 37.0 35.0 37.0 32.0 37.0 7 35.26812356325058 37.0 35.0 37.0 32.0 37.0 8 35.231546401495315 37.0 35.0 37.0 32.0 37.0 9 36.842102853112145 39.0 37.0 39.0 32.0 39.0 10-11 36.721015962989284 39.0 35.0 39.0 32.0 39.0 12-13 36.666264453960935 39.0 35.0 39.0 32.0 39.0 14-15 37.721151630617726 39.5 37.0 41.0 32.0 41.0 16-17 37.6827596403176 39.0 36.5 41.0 32.0 41.0 18-19 37.66822152648247 39.0 36.5 41.0 32.0 41.0 20-21 37.61019767528526 39.0 36.5 41.0 32.0 41.0 22-23 37.4738770398249 39.0 36.0 41.0 32.0 41.0 24-25 37.3473746487134 39.0 36.0 41.0 31.5 41.0 26-27 37.087756739417316 39.0 36.0 40.0 31.0 41.0 28-29 36.88597951784163 39.0 36.0 40.0 30.5 41.0 30-31 36.65887312567986 38.5 35.5 40.0 30.0 41.0 32-33 36.516486905029254 38.0 35.0 40.0 30.0 41.0 34-35 36.506286501777325 38.0 35.0 40.0 30.0 41.0 36-37 36.407715323037536 38.0 35.0 40.0 30.0 41.0 38-39 36.46038286038023 39.0 35.0 40.0 30.0 41.0 40-41 36.374252183956536 39.0 35.0 40.0 30.0 41.0 42-43 36.20356233476925 38.5 35.0 40.0 30.0 41.0 44-45 36.00965359207448 38.0 35.0 40.0 29.0 41.0 46-47 35.732793959112655 38.0 35.0 40.0 28.0 41.0 48-49 35.46036556702005 38.0 34.0 40.0 27.0 41.0 50-51 35.219727973008716 38.0 34.0 40.0 27.0 41.0 52-53 34.9814982967258 38.0 34.0 40.0 26.0 41.0 54-55 34.69972978515801 38.0 34.0 40.0 26.0 41.0 56-57 32.563454209861504 37.0 30.5 39.5 16.5 41.0 58-59 27.358897872234706 32.5 17.5 38.5 7.0 40.0 60-61 28.15322940105056 33.0 19.0 38.0 9.0 40.0 62-63 31.38982448944399 34.5 29.5 38.5 13.5 40.0 64-65 31.641404883352635 35.5 31.0 39.0 9.5 40.0 66-67 24.830284911762455 27.0 18.0 35.5 2.0 39.0 68-69 17.271562019104536 13.0 7.5 28.0 2.0 35.0 70-71 16.140824216160183 12.5 7.5 26.5 2.0 33.5 72-73 17.226683070190095 13.0 7.0 28.0 2.0 34.0 74-75 18.252427037851998 17.0 8.5 29.0 2.0 34.5 76-77 19.42455272307095 21.0 8.0 30.0 2.0 34.0 78-79 22.525610492149546 27.5 10.5 33.5 2.0 35.5 80-81 26.273990883719932 31.5 22.5 34.5 2.0 36.0 82-83 27.66208555000923 33.0 26.5 35.0 2.0 36.0 84-85 27.74444627391416 34.0 27.0 35.0 2.0 36.0 86-87 27.50982530783399 34.0 27.0 35.0 2.0 36.0 88-89 27.087786941905513 33.0 26.0 35.0 2.0 35.0 90-91 25.66338961550771 32.5 18.0 35.0 2.0 35.0 92-93 23.070585894171458 31.5 2.0 34.5 2.0 35.0 94-95 15.642859474155195 4.5 2.0 32.0 2.0 34.5 96-97 13.068337270211433 2.0 2.0 30.0 2.0 34.0 98-99 12.708735972283858 2.0 2.0 29.5 2.0 34.0 100-101 12.437127188036307 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4214103038117294 1101 2 0.39720690682365145 1101 3 0.3817499475339581 1101 4 0.2266203972030567 1101 5 0.4629528961921281 1101 6 0.4020094133968186 1101 7 0.441854910505306 1101 8 0.42642019849428436 1101 9 0.5533394786082297 1101 10-11 0.485309178549592 1101 12-13 0.6085273857933444 1101 14-15 0.6685142467891723 1101 16-17 0.7497043727776287 1101 18-19 0.5349011276417386 1101 20-21 0.6162722101398543 1101 22-23 0.7368311954809599 1101 24-25 0.632761508430157 1101 26-27 0.5398930050254194 1101 28-29 0.7035399936769622 1101 30-31 0.6768001253977047 1101 32-33 0.6346063665642561 1101 34-35 0.6114947456876934 1101 36-37 0.46527189159819926 1101 38-39 0.6565723830674912 1101 40-41 0.7210373886890338 1101 42-43 0.7804862298958852 1101 44-45 0.5604643540475891 1101 46-47 0.5873532946704998 1101 48-49 0.5882671950137563 1101 50-51 0.4970437339814495 1101 52-53 0.4356412721219627 1101 54-55 0.13166654084153606 1101 56-57 -2.150068198123403 1101 58-59 -9.56696805636842 1101 60-61 -10.027348567028781 1101 62-63 -3.7245546895968324 1101 64-65 -4.826859687904115 1101 66-67 -9.788815501136444 1101 68-69 -4.539941220751995 1101 70-71 -4.342452852065314 1101 72-73 -4.382116787706247 1101 74-75 -6.652643723336453 1101 76-77 -7.816187585723647 1101 78-79 -11.020459534658103 1101 80-81 -7.815712396422178 1101 82-83 -3.8968448746949953 1101 84-85 -3.1480867797989376 1101 86-87 -2.966120842294405 1101 88-89 -3.194393215313905 1101 90-91 -9.702156449067123 1101 92-93 -17.264585466326345 1101 94-95 -11.119170117495164 1101 96-97 -9.340589255051311 1101 98-99 -9.467816450699761 1101 100-101 -9.418670892329601 1102 1 0.40010350477186307 1102 2 0.3739970364028373 1102 3 0.4246689102751624 1102 4 0.34696075616959376 1102 5 0.3541886339375182 1102 6 0.3353496284403832 1102 7 0.389967444255781 1102 8 0.388536653202479 1102 9 0.5860337112858645 1102 10-11 0.5757572210084803 1102 12-13 0.4676011902489208 1102 14-15 0.47953111919741076 1102 16-17 0.4988781632721313 1102 18-19 0.529047855765846 1102 20-21 0.6562167647575379 1102 22-23 0.5727219466241209 1102 24-25 0.5173067145283881 1102 26-27 0.5387759848670441 1102 28-29 0.5964669611228786 1102 30-31 0.5182311024729884 1102 32-33 0.6991312940772048 1102 34-35 0.4682463772190033 1102 36-37 0.40842231697953224 1102 38-39 0.5249813711919558 1102 40-41 0.5296369480798973 1102 42-43 0.6416897709400757 1102 44-45 0.6119312364010696 1102 46-47 0.6451840520341534 1102 48-49 0.6174917789982004 1102 50-51 0.7332020632675054 1102 52-53 0.4573092261895155 1102 54-55 0.30481355950678335 1102 56-57 -2.7800034220044836 1102 58-59 -10.477782574153686 1102 60-61 -10.62161622818343 1102 62-63 -4.349278230813514 1102 64-65 -5.237260090241595 1102 66-67 -6.736709185720894 1102 68-69 -4.236867602803343 1102 70-71 -4.2271855084036645 1102 72-73 -4.60348379721864 1102 74-75 -5.3921471836867525 1102 76-77 -7.576649087945292 1102 78-79 -10.016476452884518 1102 80-81 -5.632850307512278 1102 82-83 -3.0528023963952933 1102 84-85 -2.5330733227313758 1102 86-87 -2.529463660888389 1102 88-89 -2.596420346142043 1102 90-91 -8.516038844053213 1102 92-93 -13.128532977024886 1102 94-95 -10.721782481179355 1102 96-97 -9.32038141841844 1102 98-99 -9.55212445341004 1102 100-101 -9.315560188610945 1103 1 0.2009982276142246 1103 2 0.2271410500351969 1103 3 0.2837475261654312 1103 4 0.18722628368905703 1103 5 0.23387701824442786 1103 6 0.17918050495821802 1103 7 0.15887283689852438 1103 8 0.16002832993770255 1103 9 0.31722227711347273 1103 10-11 0.30062035263106424 1103 12-13 0.3282068518422747 1103 14-15 0.3921029830908438 1103 16-17 0.38030731571820553 1103 18-19 0.4191401556265859 1103 20-21 0.3044577563237709 1103 22-23 0.41260193563142167 1103 24-25 0.3739782511552079 1103 26-27 0.29584889619891896 1103 28-29 0.27997766863898477 1103 30-31 0.2643161662081539 1103 32-33 0.32449251587257777 1103 34-35 0.2356234007999376 1103 36-37 0.22718039773882026 1103 38-39 0.03645456060561969 1103 40-41 0.16151879949406123 1103 42-43 0.09699321930416005 1103 44-45 0.10430780783442373 1103 46-47 0.2416248125419571 1103 48-49 0.16432356995692743 1103 50-51 0.13396471490788286 1103 52-53 0.10963630841729355 1103 54-55 0.005237232806564407 1103 56-57 -2.894534937572253 1103 58-59 -10.41069225624322 1103 60-61 -10.501020972600951 1103 62-63 -4.5042761818130685 1103 64-65 -6.070214281296661 1103 66-67 -7.20984632671021 1103 68-69 -4.644773524348462 1103 70-71 -4.351806421265344 1103 72-73 -4.620296739137748 1103 74-75 -5.1689395738446695 1103 76-77 -6.833572900859419 1103 78-79 -9.564550216018304 1103 80-81 -5.437649269469684 1103 82-83 -3.0715642046150258 1103 84-85 -2.6445419245014143 1103 86-87 -2.558231384521683 1103 88-89 -2.551520879499723 1103 90-91 -5.16222554173941 1103 92-93 -10.277348306283159 1103 94-95 -8.25506186558816 1103 96-97 -7.8133745647435395 1103 98-99 -8.090281297005358 1103 100-101 -8.213074325390657 1104 1 0.36628177011943563 1104 2 0.3207356666040404 1104 3 0.19875475165530432 1104 4 0.15983939923574297 1104 5 0.2378492933769607 1104 6 0.22493975188199755 1104 7 0.32708084563888207 1104 8 0.17794697022404904 1104 9 0.22689995993314227 1104 10-11 0.3233181588812286 1104 12-13 0.39962007639719843 1104 14-15 0.4620041081823203 1104 16-17 0.37517549413548323 1104 18-19 0.34997543128487507 1104 20-21 0.3906695885610745 1104 22-23 0.4611894046906926 1104 24-25 0.4661742501094537 1104 26-27 0.5447040223566688 1104 28-29 0.5369677811481353 1104 30-31 0.4702388310985057 1104 32-33 0.39186082049479154 1104 34-35 0.40544262606161396 1104 36-37 0.49581409679972666 1104 38-39 0.4537309100443423 1104 40-41 0.34289614080108066 1104 42-43 0.4376713728785475 1104 44-45 0.4418344288703082 1104 46-47 0.2961130299247472 1104 48-49 0.3057058445436027 1104 50-51 0.406781646317647 1104 52-53 0.4726361159290988 1104 54-55 0.3689854459291766 1104 56-57 -2.690680802585085 1104 58-59 -9.98267463433847 1104 60-61 -10.370036036341634 1104 62-63 -3.809401169859509 1104 64-65 -5.245821799089505 1104 66-67 -6.9829593780257575 1104 68-69 -4.377902179931256 1104 70-71 -3.9961838573799877 1104 72-73 -4.321072499414832 1104 74-75 -5.126217043737093 1104 76-77 -6.774916226341368 1104 78-79 -9.305555599531544 1104 80-81 -5.131802040253177 1104 82-83 -2.701969521557725 1104 84-85 -2.2257582980647825 1104 86-87 -2.08291881693021 1104 88-89 -2.034142868383018 1104 90-91 -4.634052800613052 1104 92-93 -6.8091676522090765 1104 94-95 -8.105422401320988 1104 96-97 -8.086639985449278 1104 98-99 -8.393717806476065 1104 100-101 -8.476934091375899 1105 1 0.2866089117685 1105 2 0.21932652976610711 1105 3 0.20905704333266684 1105 4 0.15323640071896705 1105 5 0.14157345129712695 1105 6 0.05101065299398755 1105 7 0.15524359763752216 1105 8 0.12397924540024974 1105 9 0.2727090015371445 1105 10-11 0.3340682063608753 1105 12-13 0.37485735317154223 1105 14-15 0.41407395681994785 1105 16-17 0.3426292772653383 1105 18-19 0.28663884673106566 1105 20-21 0.41081300139614996 1105 22-23 0.4308978109863588 1105 24-25 0.422901963246602 1105 26-27 0.44453210271906585 1105 28-29 0.45445153085528034 1105 30-31 0.4505955654028142 1105 32-33 0.489249978487841 1105 34-35 0.5382913184013063 1105 36-37 0.38808219172184977 1105 38-39 0.4840404604569528 1105 40-41 0.3782110708322435 1105 42-43 0.39035428466231537 1105 44-45 0.42403563399781063 1105 46-47 0.5069487625911933 1105 48-49 0.29348862836997114 1105 50-51 0.33797407526242296 1105 52-53 0.42906891653970547 1105 54-55 0.24248011923010182 1105 56-57 1.5924751501587764 1105 58-59 -8.804155167048517 1105 60-61 -10.023749895561465 1105 62-63 -3.8479278156076653 1105 64-65 -4.854203208868558 1105 66-67 -6.687102117378647 1105 68-69 -3.912844244196414 1105 70-71 -4.098078775990679 1105 72-73 -2.152237929920574 1105 74-75 -4.3051048348021315 1105 76-77 -6.406158004545599 1105 78-79 -9.32621753872506 1105 80-81 -4.652125653937205 1105 82-83 -2.400287104745342 1105 84-85 -1.8725964280574061 1105 86-87 -1.6694814529918816 1105 88-89 -1.8274707682276805 1105 90-91 -4.34979472849173 1105 92-93 -6.552191206973973 1105 94-95 -7.940921208044909 1105 96-97 -7.944472712162755 1105 98-99 -8.15501780455364 1105 100-101 -8.283304054070308 1106 1 0.09715807017581923 1106 2 0.09842766677540382 1106 3 0.08851031489879091 1106 4 -0.04260008144567706 1106 5 -0.0252262959499987 1106 6 0.16998491465290044 1106 7 0.060507151755430755 1106 8 -1.932885052298161E-4 1106 9 -0.059321589846227596 1106 10-11 0.06718818758036349 1106 12-13 0.16391394215256838 1106 14-15 0.1330535417020826 1106 16-17 0.20547264465171367 1106 18-19 0.09038352267261729 1106 20-21 0.0875129415488658 1106 22-23 0.059296070938273715 1106 24-25 0.24183464140029542 1106 26-27 0.13439588388692414 1106 28-29 0.23932760777785944 1106 30-31 0.398132861931515 1106 32-33 0.1616286418509958 1106 34-35 0.2257085328142736 1106 36-37 0.18367064286658064 1106 38-39 0.2027868480709074 1106 40-41 0.18973700729077336 1106 42-43 0.014341842129184101 1106 44-45 0.05120977533852766 1106 46-47 0.1821813640592893 1106 48-49 0.15106902073384276 1106 50-51 0.11650538238544783 1106 52-53 0.14134781067237867 1106 54-55 -0.05138730489336041 1106 56-57 1.2862737732926135 1106 58-59 -8.517470753334734 1106 60-61 -10.14940658419144 1106 62-63 -4.112644467563083 1106 64-65 -6.0729297736010714 1106 66-67 -7.278726927697054 1106 68-69 -4.48148743032592 1106 70-71 -4.170692106633446 1106 72-73 -4.723132460832133 1106 74-75 -5.175571770569981 1106 76-77 -7.011932454455071 1106 78-79 -9.674298316298746 1106 80-81 -5.32859351046676 1106 82-83 -3.032738030306863 1106 84-85 -2.456665490976345 1106 86-87 -2.333655670728639 1106 88-89 -2.3288445607302215 1106 90-91 -4.589384207753543 1106 92-93 -7.0942797502171455 1106 94-95 -7.648113528527001 1106 96-97 -7.574176386812697 1106 98-99 -7.214127521084209 1106 100-101 -7.718491138638953 1107 1 0.06988587802332447 1107 2 0.03627317131151031 1107 3 0.04222000984839269 1107 4 0.021599855987346928 1107 5 -0.008536697611042143 1107 6 -0.0733067169144519 1107 7 0.07036089978863913 1107 8 0.17307733969325056 1107 9 0.19691664439810808 1107 10-11 0.0035381914053758123 1107 12-13 -0.007350596457847303 1107 14-15 0.07992264642329161 1107 16-17 0.11593583650680728 1107 18-19 0.215279271932026 1107 20-21 0.2140215872780047 1107 22-23 0.16951216952337944 1107 24-25 0.32456007046801716 1107 26-27 0.19379714567638473 1107 28-29 0.29230429022442905 1107 30-31 0.22931632802310276 1107 32-33 0.10102575455466933 1107 34-35 0.1361447181333375 1107 36-37 0.1830534608453931 1107 38-39 0.09695958716638131 1107 40-41 0.03809486660060912 1107 42-43 -0.013214635151491905 1107 44-45 0.05886155372443369 1107 46-47 0.101038407771739 1107 48-49 -0.056156759859767647 1107 50-51 0.1239525724821462 1107 52-53 0.15656064752931087 1107 54-55 -0.055886263102458145 1107 56-57 1.3090619986192777 1107 58-59 -8.914491965480668 1107 60-61 -10.770284201813723 1107 62-63 -4.234981430207423 1107 64-65 -5.568451759236236 1107 66-67 -7.201354194069022 1107 68-69 -4.012302769773827 1107 70-71 -4.105064680867802 1107 72-73 -2.443274861336196 1107 74-75 -4.894144374831194 1107 76-77 -7.031696498900995 1107 78-79 -7.782688221894919 1107 80-81 -2.635745127805528 1107 82-83 -1.441676289838128 1107 84-85 -1.0870556924318784 1107 86-87 -0.9519058279728192 1107 88-89 -0.8677363976795291 1107 90-91 -0.45931852565331965 1107 92-93 -3.015997723055847 1107 94-95 -6.171650882093706 1107 96-97 -6.724476688470192 1107 98-99 -6.10508064016275 1107 100-101 -7.107134813079901 1108 1 -0.16276447990404463 1108 2 -0.13108078326639472 1108 3 -0.11219262376263828 1108 4 -0.05938307389068598 1108 5 -0.04052301428549754 1108 6 -0.16719798977786837 1108 7 -0.08250663083067877 1108 8 -0.08888156509107148 1108 9 -0.026667305387420015 1108 10-11 -0.06789549885823476 1108 12-13 -0.024942658404029316 1108 14-15 -0.13848323177507638 1108 16-17 -0.19026715269354355 1108 18-19 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86-87 1.4105504601428152 2316 88-89 1.6836941965951802 2316 90-91 2.9944619762597497 2316 92-93 5.014636807028467 2316 94-95 12.139981581610618 2316 96-97 13.78459346875151 2316 98-99 13.541378391526132 2316 100-101 12.268178859075794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 16.0 4 34.0 5 67.0 6 161.0 7 417.0 8 945.0 9 1659.0 10 2739.0 11 4316.0 12 6535.0 13 9275.0 14 11638.0 15 16144.0 16 21865.0 17 29745.0 18 33441.0 19 34450.0 20 35440.0 21 35887.0 22 36372.0 23 30429.0 24 31183.0 25 36285.0 26 51382.0 27 77325.0 28 110159.0 29 149496.0 30 184861.0 31 200678.0 32 199209.0 33 180883.0 34 159279.0 35 142431.0 36 115082.0 37 73780.0 38 27364.0 39 1837.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42867931882963 19.900845372378008 14.169659171193997 23.500816137598363 2 15.344606675338468 21.671562824279096 39.32832512435266 23.655505376029776 3 18.982699777767603 25.65459181237779 30.77577715575656 24.586931254098047 4 12.648363634060807 17.365553867355544 38.01606674944746 31.97001574913618 5 13.053415303078939 38.520202823409576 34.820246436101556 13.606135437409932 6 30.93494725038009 37.350540307899756 17.861361810707365 13.853150631012792 7 27.392647151952687 33.552106624578016 21.613154651429017 17.442091572040276 8 25.645017739418822 35.27940010902326 20.40990406635317 18.665678085204746 9 26.286686889343443 16.41636760520087 20.71228184182567 36.58466366363002 10-11 24.473977390027628 26.665460190928442 28.35175766302889 20.508804756015046 12-13 25.65998525923721 24.215380763255848 28.391751603045773 21.732882374461166 14-15 22.643409281779583 25.38983415789976 26.843826663730834 25.122929896589824 16-17 21.944713858216854 28.647206196771158 27.329914931282033 22.078165013729954 18-19 21.72630174963081 27.700470259101635 29.252469000335886 21.320758990931672 20-21 22.79035917091247 26.771680393372794 28.82486502654165 21.61309540917308 22-23 22.367286533651985 26.87070770447865 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48-49 22.58232464769567 27.191056391127795 27.93095157040868 22.295667390767857 50-51 22.319054678094403 27.37269931132664 28.036109201584186 22.27213680899477 52-53 22.593079552887556 27.408712349032267 27.737642154504854 22.260565943575326 54-55 22.15434995955941 27.087468194779824 28.035104090033087 22.723077755627678 56-57 21.424548797835318 27.473672065553867 28.785224658589932 22.31655447802088 58-59 21.08437999537622 26.872914906140764 29.850449976806846 22.192255121676162 60-61 22.286864369114223 27.067675985306387 28.470895973948977 22.17456367163041 62-63 22.568363219000034 27.110646793120512 27.88121275658738 22.439777231292066 64-65 21.888137998074853 27.206098433274484 28.352944533336842 22.552819035313817 66-67 21.608523209915106 27.043939265648604 28.9393240002195 22.40821352421679 68-69 21.766448542325342 26.612339971024312 29.396303079563264 22.224908407087078 70-71 21.618581974472583 26.637779523199285 29.234089835759175 22.509548666568957 72-73 20.907925813499055 26.62101287083807 29.782772554987314 22.688288760675558 74-75 21.47492989059403 26.944457839650926 28.918110324688207 22.662501945066836 76-77 19.904592985511012 27.18684734506987 29.66566027085944 23.242899398559675 78-79 20.514784740309484 27.36156343781025 29.02375390432777 23.0998979175525 80-81 21.675167492135042 27.417459052130138 27.86826397213773 23.03910948359709 82-83 22.130055672590508 27.31383952352037 27.512543200025526 23.04356160386359 84-85 21.995310813285997 27.079955845846275 27.449863796321917 23.47486954454581 86-87 21.964366309602305 27.041834719282814 27.79232021363944 23.201478757475442 88-89 22.172861565726304 26.889116815556473 27.497893735131623 23.4401278835856 90-91 21.286109230077578 27.09376940642559 28.284840112420824 23.335281251076015 92-93 20.341497595233758 27.36375742738315 28.824495476006756 23.470249501376337 94-95 19.390231836891353 27.31047628706986 29.275255534657703 24.024036341381077 96-97 19.509122848096986 27.252241738562148 28.883373178419696 24.35526223492117 98-99 19.681191163455026 27.21476517531495 28.54121576516024 24.562827896069784 100-101 20.185082716677858 27.14002198564293 28.364135366636418 24.310759931042796 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 263.0 1 204.0 2 150.5 3 177.0 4 232.5 5 281.0 6 326.5 7 483.0 8 713.0 9 881.5 10 993.0 11 1102.5 12 1273.5 13 1465.0 14 1655.0 15 1865.5 16 2064.5 17 2306.5 18 2542.5 19 2823.5 20 3249.0 21 3759.0 22 4484.5 23 5408.5 24 6566.0 25 8131.5 26 10296.5 27 13206.5 28 16417.0 29 20101.5 30 24329.5 31 28763.0 32 33609.5 33 39041.5 34 44607.5 35 49694.5 36 54208.5 37 58291.5 38 61833.0 39 64017.0 40 66130.5 41 68881.0 42 71887.5 43 75184.0 44 78191.5 45 81116.5 46 84122.0 47 87517.0 48 90376.5 49 89376.5 50 85005.0 51 80029.0 52 74691.0 53 69558.0 54 63820.0 55 56917.5 56 50123.5 57 43687.0 58 37365.0 59 31000.5 60 24801.0 61 19228.5 62 14724.5 63 11190.0 64 8155.0 65 5811.0 66 4079.0 67 2783.5 68 1872.5 69 1234.5 70 806.0 71 521.5 72 343.0 73 233.5 74 155.0 75 91.0 76 54.0 77 32.0 78 13.5 79 5.0 80 4.0 81 2.0 82 0.5 83 1.5 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022457011385850913 2 0.008817178006158385 3 2.4356845320879517E-4 4 0.0 5 0.1436566737025474 6 0.0 7 4.871369064175903E-5 8 0.0018511202443868432 9 0.0 10-11 0.0 12-13 0.0 14-15 7.307053596263854E-4 16-17 0.0 18-19 3.166389891714337E-4 20-21 0.0 22-23 2.4356845320879517E-4 24-25 2.4356845320879516E-5 26-27 3.653526798131927E-4 28-29 0.003994522632624241 30-31 0.014297468203356277 32-33 0.003142033046393458 34-35 0.005114937517384698 36-37 0.11469638461602163 38-39 0.11386825187511174 40-41 0.12726451680159545 42-43 0.13035783615734717 44-45 0.12621717245279765 46-47 0.11939725576295139 48-49 0.1194946831442349 50-51 0.11786277450773598 52-53 0.09340850180557295 54-55 0.08057244432146944 56-57 0.5690002635410664 58-59 1.5986858994812478 60-61 0.5344622568760592 62-63 0.004822655373534144 64-65 0.12565696501041743 66-67 1.9074576276140374 68-69 2.877542063054027 70-71 2.7822093704681046 72-73 2.6376271366433635 74-75 2.012459987792349 76-77 1.4411701807911201 78-79 0.9572483779558858 80-81 0.05589896001141849 82-83 2.679252985296747E-4 84-85 1.461410719252771E-4 86-87 3.166389891714337E-4 88-89 3.166389891714337E-4 90-91 0.25942475951268773 92-93 0.4108269100272748 94-95 2.0186953401944945 96-97 2.4281582668837998 98-99 1.4507180641569049 100-101 0.958003440160833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2052811.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.65947456162235 #Duplication Level Percentage of deduplicated Percentage of total 1 72.54549330240549 36.75116572513556 2 14.567348901711979 14.759484822331107 3 5.310703293733917 8.071123152397124 4 2.558203218801993 5.1838892354544 5 1.4045623409129087 3.5577195089845115 6 0.8443952984094527 2.566597328383631 7 0.5508899086036744 1.9535455317813617 8 0.3948018357093585 1.6000362842400042 9 0.2759173720932844 1.2580046183402487 >10 1.388500544457443 12.47997452887341 >50 0.09979870470570443 3.4598172230037694 >100 0.05399692992301425 5.120963379274852 >500 0.003656379360886347 1.2284665638079215 >1k 0.0016357486614491505 1.6992438859750836 >5k 9.622050949700886E-5 0.30996821201708424 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6359 0.30977035879094567 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4805 0.23406928353365214 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3821 0.18613501194216125 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3501 0.1705466309367984 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3258 0.15870920411085093 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2790 0.13591119689050768 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 4.871369064175903E-5 18-19 0.0 0.0 0.0 0.0 4.871369064175903E-5 20-21 0.0 0.0 0.0 9.742738128351806E-5 4.871369064175903E-5 22-23 0.0 0.0 0.0 4.627800610967108E-4 4.871369064175903E-5 24-25 0.0 0.0 0.0 7.55062204947265E-4 4.871369064175903E-5 26-27 0.0 0.0 0.0 0.0011691285754022167 4.871369064175903E-5 28-29 0.0 0.0 0.0 0.003483028880885771 4.871369064175903E-5 30-31 0.0 0.0 0.0 0.01100929408503754 4.871369064175903E-5 32-33 0.0 0.0 0.0 0.026354106637191635 4.871369064175903E-5 34-35 0.0 0.0 0.0 0.050564810886145874 4.871369064175903E-5 36-37 0.0 0.0 0.0 0.08507846070583215 4.871369064175903E-5 38-39 0.0 0.0 0.0 0.13532663260280658 4.871369064175903E-5 40-41 0.0 0.0 0.0 0.19307671285861192 4.871369064175903E-5 42-43 0.0 0.0 0.0 0.254309821995303 4.871369064175903E-5 44-45 0.0 0.0 0.0 0.3270150052781284 4.871369064175903E-5 46-47 0.0 0.0 0.0 0.41085126687259566 4.871369064175903E-5 48-49 0.0 0.0 0.0 0.4875266159427244 9.742738128351806E-5 50-51 0.0 0.0 0.0 0.5640801807862488 9.742738128351806E-5 52-53 0.0 0.0 0.0 0.6376378536553049 1.2178422660439757E-4 54-55 0.0 0.0 0.0 0.7183807958940205 1.461410719252771E-4 56-57 0.0 0.0 0.0 0.7872375976161469 1.461410719252771E-4 58-59 0.0 0.0 0.0 0.834319379621407 1.9485476256703613E-4 60-61 0.0 0.0 0.0 0.8740210374944406 1.9485476256703613E-4 62-63 1.2178422660439757E-4 0.0 0.0 0.9086808283860521 1.9485476256703613E-4 64-65 1.461410719252771E-4 0.0 0.0 0.9425612002273955 1.9485476256703613E-4 66-67 1.461410719252771E-4 0.0 0.0 0.9702549333572354 1.9485476256703613E-4 68-69 1.461410719252771E-4 0.0 0.0 1.0071068403277263 1.9485476256703613E-4 70-71 1.461410719252771E-4 0.0 0.0 1.070020571791558 1.9485476256703613E-4 72-73 1.461410719252771E-4 0.0 0.0 1.1607498206118343 1.9485476256703613E-4 74-75 1.461410719252771E-4 0.0 0.0 1.282655831442836 1.9485476256703613E-4 76-77 1.461410719252771E-4 0.0 0.0 1.4773157392473053 2.1921160788791565E-4 78-79 1.461410719252771E-4 0.0 0.0 1.7730078414427828 2.4356845320879517E-4 80-81 1.461410719252771E-4 0.0 0.0 2.134853135529769 2.4356845320879517E-4 82-83 1.461410719252771E-4 0.0 0.0 2.4869313346430824 2.4356845320879517E-4 84-85 1.461410719252771E-4 2.4356845320879516E-5 0.0 2.7720038522786563 2.4356845320879517E-4 86-87 1.461410719252771E-4 4.871369064175903E-5 0.0 2.988438779799991 2.4356845320879517E-4 88-89 1.461410719252771E-4 4.871369064175903E-5 0.0 3.188432836729733 2.4356845320879517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8210 0.0 26.187986 1 TACATGG 8165 0.0 25.751719 2 ACATGGG 8225 0.0 23.902445 3 CATGGGG 3665 0.0 19.762808 4 GTATCAA 13935 0.0 19.68297 1 AGTACTT 5415 0.0 19.515154 12-13 GAGTACT 5185 0.0 19.429718 12-13 CATGGGA 5230 0.0 19.154896 4 TCAACGC 15440 0.0 17.698267 4 GTACTTT 5795 0.0 17.627409 14-15 ATCAACG 15445 0.0 17.601183 3 CAACGCA 15750 0.0 17.260336 5 AACGCAG 15785 0.0 17.227488 6 AGTACAT 6005 0.0 16.998148 2 GCACCGT 415 4.7293724E-11 16.975834 6 TATCAAC 16280 0.0 16.787136 2 TCGCGCC 120 5.0150622E-5 16.216583 70-71 GGTATCA 10830 0.0 16.16008 1 CGCGTAA 90 0.0023000538 16.145498 94-95 ACGCAGA 16985 0.0 15.872095 7 >>END_MODULE