FastQCFastQC Report
Fri 27 May 2016
SRR939632_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939632_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958934
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24340.12425125093545775No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG63850.033.1234741
TACATGG73250.027.7344782
ACATGGG74500.026.444723
GTATCAA132000.022.172941
GAGTACT48200.021.83737612-13
CATGGGA47850.021.8185084
CATGGGG32900.020.5331174
GTACTTT53350.020.2620114-15
CGTCTTA705.0633773E-420.2304114-15
CAACGCG1708.356765E-519.4345765
AGTACTT52400.019.00613412-13
GCTTATA12300.018.742911
ATACCGT2051.679674E-518.4193026
GTATAAT9800.018.4102951
TATCAAC155000.018.2885272
AACGCGG1851.5977993E-417.8592576
GTATAGG5203.973575E-617.3481641
TCAACGC164550.017.2253534
ATCAACG163950.017.1460763
ATGGGAG22350.017.1054085
Produced by FastQC (version 0.11.5)