FastQCFastQC Report
Fri 27 May 2016
SRR939632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958934
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59750.30501282840565325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45710.23334119475183954No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35940.18346713059245487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33480.17090928025140203No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30900.15773885184493197No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26590.1357370896620305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG79800.026.3114152
GTACATG80600.026.2904641
ACATGGG80100.025.1519323
CATGGGA49950.022.5755544
TTACGGC1353.9919966E-420.955319
GTATCAA133800.019.8228991
ATGGGAG23600.019.3925865
GAGTACT54550.018.71284112-13
AGTACTT57150.018.2740212-13
CATGGGG34950.017.54984
ATCAACG150600.017.4191043
TCAACGC151600.017.3042014
CAACGCA152800.017.1374265
GTACTTT60150.017.12743814-15
AACGCAG153750.017.0811336
TATCAAC154150.017.0183852
GTGTAGC11600.016.6806431
AGTACAT59600.016.5457742
GGTATCA105600.016.491091
ATGGGAT25650.015.8192925
Produced by FastQC (version 0.11.5)