##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939632_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1958934 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.473118032562606 31.0 31.0 34.0 30.0 34.0 2 31.67670988404918 31.0 31.0 34.0 30.0 34.0 3 31.736737940124577 31.0 31.0 34.0 30.0 34.0 4 35.53218229914842 37.0 35.0 37.0 33.0 37.0 5 35.284066231940436 37.0 35.0 37.0 33.0 37.0 6 35.287063270125486 37.0 35.0 37.0 32.0 37.0 7 35.19162360753349 37.0 35.0 37.0 32.0 37.0 8 35.1565136957141 37.0 35.0 37.0 32.0 37.0 9 36.753089180135724 39.0 35.0 39.0 32.0 39.0 10-11 36.63323853687771 39.0 35.0 39.0 32.0 39.0 12-13 36.56897577968426 39.0 35.0 39.0 32.0 39.0 14-15 37.6347385874154 39.0 36.0 41.0 32.0 41.0 16-17 37.5861787584472 39.0 36.0 41.0 32.0 41.0 18-19 37.55831436893739 39.0 36.0 41.0 32.0 41.0 20-21 37.496859516451295 39.0 36.0 41.0 32.0 41.0 22-23 37.360730632068254 39.0 36.0 41.0 31.5 41.0 24-25 37.233891238806414 39.0 36.0 40.5 31.0 41.0 26-27 36.94965220880336 39.0 36.0 40.0 31.0 41.0 28-29 36.752535562709106 39.0 36.0 40.0 30.0 41.0 30-31 36.51803889258137 38.0 35.0 40.0 30.0 41.0 32-33 36.36758997495576 38.0 35.0 40.0 30.0 41.0 34-35 36.347069885968594 38.0 35.0 40.0 30.0 41.0 36-37 36.27740623216505 38.0 35.0 40.0 30.0 41.0 38-39 36.3367765325427 38.0 35.0 40.0 30.0 41.0 40-41 36.24346864161835 38.0 35.0 40.0 30.0 41.0 42-43 36.07922038210578 38.0 35.0 40.0 29.5 41.0 44-45 35.88845795723593 38.0 35.0 40.0 29.0 41.0 46-47 35.612635749851705 38.0 34.5 40.0 27.5 41.0 48-49 35.340604634969836 38.0 34.0 40.0 27.0 41.0 50-51 35.11353649484873 38.0 34.0 40.0 26.5 41.0 52-53 34.88167927046037 38.0 34.0 40.0 26.0 41.0 54-55 34.60419442411026 38.0 33.0 40.0 26.0 41.0 56-57 33.75433398981283 37.5 32.5 40.0 22.0 41.0 58-59 30.49860076960224 35.5 26.5 39.0 8.0 40.5 60-61 30.226337130296375 34.5 25.5 39.0 10.5 40.0 62-63 32.18195151036227 35.5 30.5 39.0 19.5 40.0 64-65 32.26536243691722 36.0 31.0 39.0 18.5 40.0 66-67 27.19625827108009 31.5 18.5 37.0 4.5 39.5 68-69 19.55057393459912 19.0 8.0 31.5 2.0 37.0 70-71 17.639355384101762 15.5 7.5 29.0 2.0 35.0 72-73 19.327658308038963 19.5 7.5 30.5 2.0 35.5 74-75 20.789425013808533 22.5 9.0 32.0 2.0 36.0 76-77 22.66510306115469 27.0 11.5 33.0 2.0 35.5 78-79 24.911928885812387 30.5 13.0 34.5 2.0 36.5 80-81 26.571227769797247 32.0 22.5 35.0 2.0 36.5 82-83 27.783977408120947 33.0 26.5 35.0 2.0 36.0 84-85 27.86441018431453 34.0 27.0 35.0 2.0 36.0 86-87 27.594278316676316 33.5 27.0 35.0 2.0 36.0 88-89 27.162309194694664 33.0 26.0 35.0 2.0 35.0 90-91 25.26517662157071 32.0 16.0 34.5 2.0 35.0 92-93 23.555216000130685 31.0 2.0 34.0 2.0 35.0 94-95 19.664884830218885 27.0 2.0 34.0 2.0 35.0 96-97 16.94803296078377 16.5 2.0 33.0 2.0 35.0 98-99 17.22061105682989 20.0 2.0 33.0 2.0 35.0 100-101 16.456954394583995 10.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.16789352235396038 1101 2 0.17246140988103065 1101 3 0.19384273838349486 1101 4 0.1512281256629322 1101 5 0.16859260832200818 1101 6 0.2687773918943037 1101 7 0.17288773130826485 1101 8 0.2837147223887939 1101 9 0.3097199189421076 1101 10-11 0.2041005580542432 1101 12-13 0.223789040898275 1101 14-15 0.36260800865377973 1101 16-17 0.33792663960446845 1101 18-19 0.25275724733846516 1101 20-21 0.34654659744860083 1101 22-23 0.2914711231941354 1101 24-25 0.2515743159493553 1101 26-27 0.2955798763187971 1101 28-29 0.2954624822355214 1101 30-31 0.1922728044576374 1101 32-33 0.33541322176024835 1101 34-35 0.11423550015310724 1101 36-37 -0.021492440723498873 1101 38-39 -0.01025699194829599 1101 40-41 0.02561886182119366 1101 42-43 0.20644786693687678 1101 44-45 0.019126107739133147 1101 46-47 0.03684348496490486 1101 48-49 -0.08670146350090846 1101 50-51 -0.05336506512357886 1101 52-53 -0.14867698835173115 1101 54-55 -0.024785640426770783 1101 56-57 -3.512962929064585 1101 58-59 -10.083128279655593 1101 60-61 -12.272658073538441 1101 62-63 -4.396307095624689 1101 64-65 -1.4081064225242699 1101 66-67 -2.629862105105495 1101 68-69 -4.973867187146974 1101 70-71 -4.689756240413686 1101 72-73 -3.6131120242058685 1101 74-75 -6.437546294116542 1101 76-77 -5.116492753979639 1101 78-79 0.9096855722713499 1101 80-81 1.4882979101140208 1101 82-83 0.1751796638620533 1101 84-85 -0.1945523836398415 1101 86-87 -0.08712180049303342 1101 88-89 -0.16767888428528366 1101 90-91 1.2743345908315185 1101 92-93 0.7525983493478385 1101 94-95 -8.683474001198997 1101 96-97 -9.773509233104068 1101 98-99 -8.932722738695446 1101 100-101 -9.624857354566425 1102 1 0.2846507476834077 1102 2 0.383517255009 1102 3 0.33871330537475686 1102 4 0.24179822081169533 1102 5 0.43585241493774873 1102 6 0.3350149731248706 1102 7 0.3382611110913203 1102 8 0.4160698449623865 1102 9 0.39223919606190094 1102 10-11 0.36301450574600835 1102 12-13 0.37381167699135176 1102 14-15 0.5054404698401669 1102 16-17 0.5466464227781316 1102 18-19 0.4428768499378464 1102 20-21 0.4626457469659968 1102 22-23 0.5570638758716697 1102 24-25 0.42840743221056243 1102 26-27 0.29342044448472393 1102 28-29 0.5234641010591545 1102 30-31 0.43297871616486816 1102 32-33 0.33437702030245475 1102 34-35 0.3470174931410597 1102 36-37 0.28723814604004616 1102 38-39 0.39734723482762746 1102 40-41 0.33387979252473343 1102 42-43 0.4638879820379884 1102 44-45 0.3930623614142803 1102 46-47 0.31535060032923923 1102 48-49 0.3650049892432392 1102 50-51 0.45399295126872374 1102 52-53 0.45897821250501636 1102 54-55 0.27701843046878594 1102 56-57 -3.240282892812534 1102 58-59 -9.803793317781118 1102 60-61 -7.705975916010093 1102 62-63 -1.9136603618671586 1102 64-65 -0.14682631716354422 1102 66-67 -1.3951881977471352 1102 68-69 -4.464181254364167 1102 70-71 -4.467695309881627 1102 72-73 -3.0052512550417134 1102 74-75 -3.67370505201238 1102 76-77 -3.715221703056148 1102 78-79 2.440806341829923 1102 80-81 2.693261516592404 1102 82-83 1.130218080913206 1102 84-85 1.000990878306915 1102 86-87 0.7442791764601644 1102 88-89 0.6863322485568553 1102 90-91 2.06668680672497 1102 92-93 1.7378977502630555 1102 94-95 -8.364886639169843 1102 96-97 -9.375386013874364 1102 98-99 -8.25532179089479 1102 100-101 -9.181583275208055 1103 1 0.1436157577904318 1103 2 0.22659145737030784 1103 3 0.20817046144085083 1103 4 0.2624322881081156 1103 5 0.24746857890377782 1103 6 0.2138038388581407 1103 7 0.14351634151416448 1103 8 0.31110037752798547 1103 9 0.28113677946971904 1103 10-11 0.3658213784389375 1103 12-13 0.22924937951783875 1103 14-15 0.33781621940757134 1103 16-17 0.35050551320080814 1103 18-19 0.2892906640150983 1103 20-21 0.323321380172537 1103 22-23 0.3941023079481809 1103 24-25 0.5327144125510301 1103 26-27 0.517056524726776 1103 28-29 0.5088228719230017 1103 30-31 0.3243562113566796 1103 32-33 0.246763551543296 1103 34-35 0.20258007950759094 1103 36-37 0.32992399243948256 1103 38-39 0.36500919362378426 1103 40-41 0.29265786812504047 1103 42-43 0.23640440093200965 1103 44-45 0.29104516487778653 1103 46-47 0.26658478259069796 1103 48-49 0.33269465300644896 1103 50-51 0.22246243583367686 1103 52-53 0.4858429365529702 1103 54-55 0.3840961190031962 1103 56-57 0.5392526112084894 1103 58-59 -8.377630967520119 1103 60-61 -8.030088606280831 1103 62-63 -2.4047139838791978 1103 64-65 -0.5115560753012929 1103 66-67 -1.960155393797212 1103 68-69 -3.576827852765229 1103 70-71 -4.104410228308474 1103 72-73 -3.2053036406931668 1103 74-75 -3.870955006225163 1103 76-77 -0.5238570452040285 1103 78-79 3.23702605194714 1103 80-81 2.6290369617012495 1103 82-83 1.1964745096287501 1103 84-85 0.7345031448396959 1103 86-87 0.6131591809606327 1103 88-89 0.45294888414179013 1103 90-91 1.8503924370087397 1103 92-93 1.5326962590167703 1103 94-95 -7.354960858873191 1103 96-97 -8.877644180177764 1103 98-99 -8.072027420755585 1103 100-101 -9.48830496549021 1104 1 0.23351173709636797 1104 2 0.21889595815988372 1104 3 0.21446949235917145 1104 4 0.12187247303379678 1104 5 0.24063970712823135 1104 6 0.11907218854040025 1104 7 0.24287811930699377 1104 8 0.2505365828259727 1104 9 0.219899199930083 1104 10-11 0.2754048722980329 1104 12-13 0.18064553028645491 1104 14-15 0.38447767163297897 1104 16-17 0.3625921505424117 1104 18-19 0.27591530468434655 1104 20-21 0.32317076819894197 1104 22-23 0.3833704374808349 1104 24-25 0.3448467732746394 1104 26-27 0.3037335567712489 1104 28-29 0.36890889961951956 1104 30-31 0.21070746430435605 1104 32-33 0.3852572412932105 1104 34-35 0.11738471789067972 1104 36-37 0.015510331309009473 1104 38-39 0.05168506141547624 1104 40-41 0.13624505795750963 1104 42-43 0.15382945480516952 1104 44-45 0.1648622840506988 1104 46-47 -0.005363499183879128 1104 48-49 0.06381131960255715 1104 50-51 0.33087511475816456 1104 52-53 0.31935838724580634 1104 54-55 0.3048800421540534 1104 56-57 0.7739375009008356 1104 58-59 -8.085949659492702 1104 60-61 -7.639911830371972 1104 62-63 -2.134477682900801 1104 64-65 -0.5206715395418016 1104 66-67 -1.45722391113188 1104 68-69 -3.4702427331858843 1104 70-71 -3.5805553238407075 1104 72-73 -2.48775972719503 1104 74-75 -3.2835485168918375 1104 76-77 -0.1111123933579492 1104 78-79 3.719944452644846 1104 80-81 3.0539847820083565 1104 82-83 1.426644850394954 1104 84-85 0.9618943083727594 1104 86-87 1.187512385619339 1104 88-89 0.9103235787900381 1104 90-91 2.2138605131974423 1104 92-93 2.044829148946196 1104 94-95 -6.351889136668142 1104 96-97 -7.88730610233794 1104 98-99 -5.858642959084904 1104 100-101 -5.397663758562272 1105 1 0.3261722875550852 1105 2 0.3689577708998151 1105 3 0.3859824947232404 1105 4 0.27410206940744786 1105 5 0.345599908399187 1105 6 0.41052251061731226 1105 7 0.21423279430211295 1105 8 0.25772746490461174 1105 9 0.43716122088135023 1105 10-11 0.3099972335446992 1105 12-13 0.3204198595640051 1105 14-15 0.3860961894285424 1105 16-17 0.5004367294566734 1105 18-19 0.5103938003574555 1105 20-21 0.46507709478709813 1105 22-23 0.5844483649105072 1105 24-25 0.6193365470617067 1105 26-27 0.4838065391817281 1105 28-29 0.47929560879600075 1105 30-31 0.5130167665795042 1105 32-33 0.32399051389823796 1105 34-35 0.3635273860321746 1105 36-37 0.526466368353276 1105 38-39 0.6064684999389058 1105 40-41 0.41224165283464487 1105 42-43 0.42673703933679263 1105 44-45 0.572281439493473 1105 46-47 0.5670051216329242 1105 48-49 0.5762703822177784 1105 50-51 0.5717018872655899 1105 52-53 0.6646823076917272 1105 54-55 0.379492899203953 1105 56-57 1.0700080541790769 1105 58-59 -8.148534516455854 1105 60-61 -7.28556921428331 1105 62-63 -1.7883832720738155 1105 64-65 0.13855590965246023 1105 66-67 -1.1502558802471974 1105 68-69 -2.9202899060663583 1105 70-71 -3.418365200865889 1105 72-73 -2.483546158756397 1105 74-75 -3.360403983523799 1105 76-77 0.3004297802816289 1105 78-79 4.367263189496086 1105 80-81 3.8318525678109445 1105 82-83 2.339696556209688 1105 84-85 1.7619967873889593 1105 86-87 1.6817833206183614 1105 88-89 1.7960659823296332 1105 90-91 3.075242732817024 1105 92-93 2.8266448168491145 1105 94-95 -5.88180975609686 1105 96-97 -7.598328832075291 1105 98-99 -5.311607801733526 1105 100-101 -4.812603464029987 1106 1 0.16392252587729672 1106 2 0.2725897337959182 1106 3 0.26732935889873133 1106 4 0.24256015286867694 1106 5 0.32733214466157534 1106 6 0.329904698313797 1106 7 0.32335799966487144 1106 8 0.3039509311349775 1106 9 0.28811619995632043 1106 10-11 0.2967428000754708 1106 12-13 0.22829576073746694 1106 14-15 0.34631091605099584 1106 16-17 0.37457341710471326 1106 18-19 0.34421189121061957 1106 20-21 0.3156824219364438 1106 22-23 0.3450184902353115 1106 24-25 0.39584597740964966 1106 26-27 0.3781528376941026 1106 28-29 0.4855354815105244 1106 30-31 0.5044011087538536 1106 32-33 0.41521259414127343 1106 34-35 0.3595642510415118 1106 36-37 0.3907182619725518 1106 38-39 0.6095357011684897 1106 40-41 0.45593112857085316 1106 42-43 0.416243199656094 1106 44-45 0.4276195189883012 1106 46-47 0.40039209154910793 1106 48-49 0.39680079844217175 1106 50-51 0.4529590566512738 1106 52-53 0.5671330262641803 1106 54-55 0.6933717030630788 1106 56-57 1.422976996794695 1106 58-59 -8.265535284946392 1106 60-61 -7.054485124291038 1106 62-63 -1.5147251444412824 1106 64-65 0.22959476913138133 1106 66-67 0.9861321466251844 1106 68-69 -2.0292379754874226 1106 70-71 -2.044417368076809 1106 72-73 -0.6651538815228761 1106 74-75 -0.5246429187249468 1106 76-77 2.8530568460225005 1106 78-79 5.072543913905463 1106 80-81 4.1505170845070225 1106 82-83 2.4149344840346814 1106 84-85 1.9827916680250262 1106 86-87 1.8595950156470629 1106 88-89 1.7900720941957111 1106 90-91 3.0641722998706413 1106 92-93 2.439107885357455 1106 94-95 -6.101992774033565 1106 96-97 -8.009753649586958 1106 98-99 -5.815181733452803 1106 100-101 -5.1667258185407565 1107 1 -0.04762932611363979 1107 2 0.016032196909701923 1107 3 0.09503427641232776 1107 4 0.05886994324013983 1107 5 0.02382637629131068 1107 6 -0.013975363742723346 1107 7 -0.06660817147558618 1107 8 0.019608440286688733 1107 9 -0.029115660974809998 1107 10-11 -0.053258647729137465 1107 12-13 -0.07556302311570562 1107 14-15 5.953092150008388E-4 1107 16-17 0.027336145003864942 1107 18-19 -0.01374693956047679 1107 20-21 -0.026475996843750238 1107 22-23 0.07168723208858552 1107 24-25 0.1918939685128862 1107 26-27 -0.007198003819709697 1107 28-29 -0.1121019728327326 1107 30-31 -0.060578398024354385 1107 32-33 0.016013501552805565 1107 34-35 -0.05900024616534694 1107 36-37 -0.004408201497753339 1107 38-39 0.08724629679078788 1107 40-41 -0.17306826199389747 1107 42-43 -0.1605945055193132 1107 44-45 -0.24142094398342095 1107 46-47 -0.04054766032432866 1107 48-49 -0.18866491731849067 1107 50-51 -0.20458945724517008 1107 52-53 -0.2932385482640498 1107 54-55 -0.3166281023881794 1107 56-57 0.11010495059886694 1107 58-59 -8.87191431204884 1107 60-61 -8.29793652307389 1107 62-63 -2.893520824373308 1107 64-65 -1.0648837112890988 1107 66-67 0.13047883158244744 1107 68-69 -2.5633867093596727 1107 70-71 -2.858917968237062 1107 72-73 -1.4352785917922901 1107 74-75 -1.3342191734625501 1107 76-77 1.794952035827425 1107 78-79 3.7303367448294864 1107 80-81 2.719662535934763 1107 82-83 1.169083009785016 1107 84-85 0.7084447975677968 1107 86-87 0.6146231958064412 1107 88-89 0.7026071638690468 1107 90-91 1.8927193193612588 1107 92-93 1.172943712458821 1107 94-95 -7.06704808695617 1107 96-97 -8.516803779369068 1107 98-99 -6.564294500691519 1107 100-101 -5.77713596348862 1108 1 -0.19565703823463565 1108 2 -0.16064588855427786 1108 3 -0.14948618892324106 1108 4 -0.1053790397976968 1108 5 -0.19082978320219013 1108 6 -0.16523761858709918 1108 7 -0.1659893008557276 1108 8 -0.05319304963924054 1108 9 -0.15131872133779467 1108 10-11 -0.23678017975894505 1108 12-13 -0.12459336516272401 1108 14-15 -0.18306327970985592 1108 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20-21 -0.6019856965338235 2315 22-23 -0.5515518408685338 2315 24-25 -0.36522129120554325 2315 26-27 -0.2973152676539712 2315 28-29 -0.4587347507909101 2315 30-31 -0.41382639150666023 2315 32-33 -0.4474322806768498 2315 34-35 -0.5976906823247461 2315 36-37 -0.43759157041071006 2315 38-39 -0.6236469038187167 2315 40-41 -0.762478152680238 2315 42-43 -0.4685723949144105 2315 44-45 -0.3797338790811935 2315 46-47 -0.30187809636843355 2315 48-49 -0.31542344249675835 2315 50-51 -0.1719579647649212 2315 52-53 -0.33744666791150024 2315 54-55 -0.4071686708422817 2315 56-57 0.37740293937351765 2315 58-59 3.463977447441895 2315 60-61 3.4949500906620905 2315 62-63 0.8349118724979263 2315 64-65 0.08560979983345618 2315 66-67 -1.7180622278127835 2315 68-69 -4.585155168649987 2315 70-71 -3.627911673430278 2315 72-73 -2.900976122099017 2315 74-75 -0.2844093050655161 2315 76-77 2.163424248175314 2315 78-79 3.94165219323839 2315 80-81 2.9130499864803703 2315 82-83 1.147002908560836 2315 84-85 0.7288533211127941 2315 86-87 0.7713969824324742 2315 88-89 1.0866426182730073 2315 90-91 2.5091888569927114 2315 92-93 3.9630375581130757 2315 94-95 6.48478907526961 2315 96-97 4.297925391180613 2315 98-99 6.574012669931182 2315 100-101 6.854240746879173 2316 1 -0.41740949298321794 2316 2 -0.3909669020220825 2316 3 -0.44256984511494935 2316 4 -0.31605181514976977 2316 5 -0.4608675012597132 2316 6 -0.3991510720442477 2316 7 -0.3542157321945112 2316 8 -0.431659933594716 2316 9 -0.5578896579345596 2316 10-11 -0.6243690939298645 2316 12-13 -0.5486908991397286 2316 14-15 -0.6523558611419134 2316 16-17 -0.7104908361424904 2316 18-19 -0.6984384096773439 2316 20-21 -0.6405797778910554 2316 22-23 -0.7684857936434426 2316 24-25 -0.6705985512452699 2316 26-27 -0.6525820927434438 2316 28-29 -0.6687162262186064 2316 30-31 -0.6171849132290248 2316 32-33 -0.49255868364078026 2316 34-35 -0.4156280843582607 2316 36-37 -0.45413323812514506 2316 38-39 -0.5456870585533764 2316 40-41 -0.5638765064241298 2316 42-43 -0.49597157849208173 2316 44-45 -0.5189568319483158 2316 46-47 -0.5071608475608045 2316 48-49 -0.546886820842289 2316 50-51 -0.6501014840983785 2316 52-53 -0.4211472446040645 2316 54-55 -0.10921905181142222 2316 56-57 0.5803989327013142 2316 58-59 3.5732237027121734 2316 60-61 3.695661421665182 2316 62-63 0.9570849892362645 2316 64-65 0.19539775215573485 2316 66-67 -2.016943838193246 2316 68-69 -3.1151921130175957 2316 70-71 -3.1901869267810454 2316 72-73 -0.9084907280117918 2316 74-75 5.470009140714485 2316 76-77 5.849108986777669 2316 78-79 4.989789363086089 2316 80-81 3.264218983387355 2316 82-83 1.5584244389755 2316 84-85 1.2293473446331369 2316 86-87 1.1019652509298048 2316 88-89 1.0163723564981701 2316 90-91 2.6137028593900062 2316 92-93 4.403170080053382 2316 94-95 7.078084081179028 2316 96-97 4.083607719912141 2316 98-99 6.2239647868455386 2316 100-101 6.203804036373816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 13.0 4 35.0 5 77.0 6 161.0 7 438.0 8 928.0 9 1608.0 10 2559.0 11 4195.0 12 6134.0 13 8395.0 14 10528.0 15 14295.0 16 19010.0 17 25066.0 18 27424.0 19 27677.0 20 29966.0 21 30641.0 22 30400.0 23 30972.0 24 32768.0 25 34676.0 26 43330.0 27 61976.0 28 88003.0 29 116939.0 30 141438.0 31 156741.0 32 161775.0 33 160253.0 34 163704.0 35 173030.0 36 172588.0 37 128985.0 38 49236.0 39 2970.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37077053872097 19.91656603359315 14.137819228108158 23.574844199577726 2 15.33618014562854 21.708628557366637 39.29029827200127 23.664893025003547 3 18.980840541357313 25.69262372072889 30.819458397654227 24.50707734025957 4 12.613288655973095 17.41528811077862 38.041608344130026 31.929814889118262 5 13.044468804817225 38.55204373424763 34.80876483034181 13.594722630593337 6 30.948566924664128 37.41233752643019 17.792789343591974 13.846306205313708 7 27.292088452188796 33.51731094564697 21.684497793187518 17.50610280897672 8 25.61907489339849 35.35259792202992 20.377797890788393 18.65052929378319 9 26.214972020496862 16.422145922220963 20.720912496286246 36.64196956099593 10-11 24.492708789576373 26.660571514915766 28.37655582066573 20.470163874842136 12-13 25.682182248100244 24.24216436082073 28.38673993100329 21.688913460075735 14-15 22.607717297863523 25.436387527030146 26.84621761742671 25.10967755767962 16-17 21.91424568521596 28.687829372462108 27.31963591413389 22.078289028188042 18-19 21.71789206727775 27.754267138490313 29.235794752629506 21.29204604160243 20-21 22.766387228972494 26.829183627421855 28.839281976830257 21.5651471667754 22-23 22.317648271968327 26.865529925969938 28.59700735195775 22.219814450103986 24-25 22.3377471307084 26.85170957863286 28.85859291767508 21.95195037298366 26-27 22.32544451494768 26.831775257244633 28.968040254567157 21.874739973240533 28-29 21.999289401100917 27.26359186303002 28.92020107090113 21.81691766496793 30-31 22.160818822419497 27.03736905973158 28.807420616465173 21.994391501383745 32-33 22.067992190110417 27.03813077251182 28.738765115452154 22.15511192192561 34-35 22.61538665735528 26.934001466134937 28.502388084692775 21.948223791817007 36-37 22.52423974869144 26.69468748914219 28.691477664073055 22.08959509809332 38-39 22.338631083226574 26.999390735345397 28.92224020354804 21.73973797787999 40-41 22.30571984703201 26.859401836811493 28.915282824466193 21.919595491690306 42-43 22.041163576162322 27.125371090456653 28.501637652204572 22.331827681176453 44-45 22.378470687487003 27.14135484111626 28.150375294392376 22.329799177004357 46-47 22.303538412447153 27.195786228291613 27.931106457415577 22.569568901845656 48-49 22.52191450310309 27.228624817831502 27.98407007531973 22.265390603745676 50-51 22.295174593978924 27.346385575252185 28.01531999806891 22.34311983269998 52-53 22.585914764870633 27.47213903079533 27.699092848783778 22.242853355550256 54-55 22.137905778736627 27.10263332894088 28.02676027707209 22.732700615250405 56-57 22.22405276036638 27.571582555567474 27.859036643116582 22.345328040949564 58-59 21.438447378704677 26.919952283992515 29.377506632936385 22.264093704366424 60-61 21.716567244570673 27.067969778687978 29.062823273350535 22.15263970339082 62-63 22.549048636153866 27.087145050825168 27.922906652399952 22.44089966062101 64-65 22.253436068464335 27.193661099138616 28.005652184899755 22.54725064749729 66-67 21.871600266881067 27.148515183700123 28.515364373958707 22.464520175460105 68-69 21.27527505699332 26.642020765204776 29.897235533083315 22.185468644718583 70-71 22.09506301450345 26.67553868501425 28.806783226031047 22.42261507445125 72-73 21.457755308255635 26.682187828764288 29.202548305847653 22.657508557132424 74-75 21.564755963261 27.035346069402376 28.705232393224474 22.694665574112147 76-77 20.585975310572262 27.157701893829906 29.15347006707764 23.102852728520187 78-79 21.707606146197183 27.115909524387718 28.224714013205332 22.951770316209767 80-81 21.754133929695428 27.44647921742286 27.749988573074486 23.04939827980723 82-83 22.108466839431006 27.33887189974325 27.546357566029013 23.006303694796728 84-85 22.00540814274148 27.083901254685035 27.426798128335424 23.483892474238065 86-87 21.98756340305968 27.062539307855037 27.763648504055343 23.186248785029935 88-89 22.195756567478316 26.88065390699937 27.525877607861016 23.397711917661297 90-91 21.685141307699322 27.027831301481353 28.013871090336746 23.273156300482583 92-93 21.54496419413826 27.183774479257117 27.91552166229096 23.355739664313663 94-95 20.459467845703912 27.17059409910964 28.546131825243258 23.82380622994319 96-97 20.2190296902703 27.112386255126452 28.524394266939122 24.144189787664125 98-99 20.80624364317679 26.89001408102976 28.102996785332508 24.200745490460942 100-101 20.152637303873156 27.015668069316273 28.627683944047703 24.204010682762863 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 292.0 1 239.0 2 196.5 3 232.5 4 252.0 5 288.0 6 387.5 7 552.0 8 782.5 9 985.5 10 1134.0 11 1240.0 12 1327.0 13 1451.0 14 1557.5 15 1682.5 16 1930.5 17 2161.0 18 2374.0 19 2610.0 20 2914.5 21 3434.0 22 4021.0 23 4756.0 24 5863.5 25 7513.5 26 9765.5 27 12453.5 28 15558.0 29 18824.5 30 22582.5 31 26990.5 32 31393.0 33 36279.5 34 41676.0 35 46583.5 36 51156.5 37 55289.5 38 58040.5 39 59669.0 40 61373.0 41 64068.0 42 67285.0 43 70348.5 44 73211.0 45 75626.5 46 78399.0 47 82685.5 48 87609.5 49 86923.5 50 81110.0 51 75925.5 52 71054.0 53 66663.5 54 62055.5 55 56065.0 56 49555.5 57 43326.5 58 37587.5 59 31640.0 60 25387.0 61 19835.5 62 15304.5 63 11633.5 64 8551.5 65 6127.0 66 4312.5 67 3005.0 68 2070.0 69 1330.5 70 880.0 71 590.5 72 384.5 73 271.0 74 182.5 75 117.0 76 70.5 77 35.5 78 16.5 79 8.0 80 4.0 81 2.5 82 2.5 83 2.0 84 1.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025473037886932382 2 0.012864139373761442 3 3.062890327086058E-4 4 0.0 5 0.06595423837658645 6 0.0 7 0.0 8 1.531445163543029E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 5.104817211810097E-4 16-17 2.5524086059050482E-5 18-19 5.104817211810097E-4 20-21 0.0 22-23 0.0 24-25 5.1048172118100964E-5 26-27 5.1048172118100964E-5 28-29 0.0011741079587163222 30-31 0.0061768288262902165 32-33 0.006917027322002681 34-35 0.0021440232289602406 36-37 0.09280557691070755 38-39 0.08456129711363425 40-41 0.09150384852169599 42-43 0.09515379282814021 44-45 0.09888030939276159 46-47 0.09329053454582951 48-49 0.0822641293683197 50-51 0.07618939688626569 52-53 0.06467803407363393 54-55 0.05268171362588019 56-57 0.07167163365381375 58-59 0.719472937832515 60-61 0.34383496330146907 62-63 0.00316498667132226 64-65 0.002322691831373594 66-67 1.1098638341056921 68-69 2.3813206570512326 70-71 2.3381594275253788 72-73 1.8141754648191313 74-75 1.2884303401748092 76-77 0.7463242763666361 78-79 0.5697231249240658 80-81 0.04285494049314576 82-83 1.7866860241335338E-4 84-85 0.0018377341962516349 86-87 0.0019398305404878368 88-89 5.1048172118100964E-5 90-91 0.33324246758696313 92-93 0.05890959062428852 94-95 0.9607265992626602 96-97 1.6605204667436473 98-99 0.6482352136417051 100-101 0.577380350741781 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1958934.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.37909889774247 #Duplication Level Percentage of deduplicated Percentage of total 1 73.18647047843174 37.60254904688054 2 14.262785667883806 14.656181507750118 3 5.2036595029510355 8.02078008696896 4 2.446567159710475 5.028096642349335 5 1.3415143035530166 3.4462898037493273 6 0.8513688307076099 2.6245538010828993 7 0.541092367190388 1.946058677268197 8 0.38800933964620876 1.5948456187944247 9 0.28546980516319315 1.3200463216619076 >10 1.3404880625569695 12.268558192344496 >50 0.09389173210262985 3.2940113441984367 >100 0.05361135114078325 5.111010819855479 >500 0.0033809326414530414 1.131511546825056 >1k 0.0015910271253896662 1.6502380892423039 >5k 9.943919533685414E-5 0.30526850102856856 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5975 0.30501282840565325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4571 0.23334119475183954 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3594 0.18346713059245487 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3348 0.17090928025140203 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3090 0.15773885184493197 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2659 0.1357370896620305 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.5524086059050482E-5 0.0 14-15 0.0 0.0 0.0 5.1048172118100964E-5 0.0 16-17 0.0 0.0 0.0 1.531445163543029E-4 0.0 18-19 0.0 0.0 0.0 2.2971677453145436E-4 0.0 20-21 0.0 0.0 0.0 3.062890327086058E-4 0.0 22-23 0.0 0.0 0.0 4.3390946300385824E-4 2.5524086059050482E-5 24-25 0.0 0.0 0.0 5.360058072400602E-4 5.1048172118100964E-5 26-27 0.0 0.0 0.0 8.933430120667669E-4 5.1048172118100964E-5 28-29 0.0 0.0 0.0 0.002960793982849856 5.1048172118100964E-5 30-31 0.0 0.0 0.0 0.00946943592790773 5.1048172118100964E-5 32-33 0.0 0.0 0.0 0.02327796648585404 5.1048172118100964E-5 34-35 0.0 0.0 0.0 0.045126584152401256 5.1048172118100964E-5 36-37 0.0 0.0 0.0 0.07664883043532861 5.1048172118100964E-5 38-39 0.0 0.0 0.0 0.12698232814377616 5.1048172118100964E-5 40-41 0.0 0.0 0.0 0.18632582823106852 5.1048172118100964E-5 42-43 0.0 0.0 0.0 0.2475325866006716 5.1048172118100964E-5 44-45 0.0 0.0 0.0 0.32037832821320167 5.1048172118100964E-5 46-47 0.0 0.0 0.0 0.4042759990893006 5.1048172118100964E-5 48-49 0.0 0.0 0.0 0.49054740996889123 5.1048172118100964E-5 50-51 0.0 0.0 0.0 0.578350266012025 1.0209634423620193E-4 52-53 0.0 0.0 0.0 0.6647492973219108 1.2762043029525242E-4 54-55 5.1048172118100964E-5 0.0 0.0 0.7611282462808855 1.531445163543029E-4 56-57 5.1048172118100964E-5 0.0 0.0 0.8521471371674594 1.531445163543029E-4 58-59 5.1048172118100964E-5 0.0 0.0 0.9197604411378841 1.531445163543029E-4 60-61 5.1048172118100964E-5 0.0 0.0 0.9771641106846887 1.531445163543029E-4 62-63 7.657225817715145E-5 0.0 0.0 1.028441999577321 1.531445163543029E-4 64-65 1.0209634423620193E-4 0.0 0.0 1.0859222413823029 1.531445163543029E-4 66-67 1.0209634423620193E-4 0.0 0.0 1.1338309509151405 1.531445163543029E-4 68-69 1.0209634423620193E-4 0.0 0.0 1.1941188421866178 1.531445163543029E-4 70-71 1.0209634423620193E-4 0.0 0.0 1.288736629207518 1.531445163543029E-4 72-73 1.0209634423620193E-4 0.0 0.0 1.4127836874545032 1.531445163543029E-4 74-75 1.0209634423620193E-4 0.0 0.0 1.5851223165252122 1.531445163543029E-4 76-77 1.0209634423620193E-4 0.0 0.0 1.808483593627963 1.531445163543029E-4 78-79 1.0209634423620193E-4 0.0 0.0 2.0919540933997776 1.531445163543029E-4 80-81 1.0209634423620193E-4 0.0 0.0 2.4362740143363686 1.531445163543029E-4 82-83 1.0209634423620193E-4 0.0 0.0 2.7754891180611496 1.531445163543029E-4 84-85 1.0209634423620193E-4 0.0 0.0 3.079685175712913 1.531445163543029E-4 86-87 1.0209634423620193E-4 0.0 0.0 3.3632322477429053 1.531445163543029E-4 88-89 1.0209634423620193E-4 0.0 0.0 3.6374630283613434 1.531445163543029E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 7980 0.0 26.311415 2 GTACATG 8060 0.0 26.290464 1 ACATGGG 8010 0.0 25.151932 3 CATGGGA 4995 0.0 22.575554 4 TTACGGC 135 3.9919966E-4 20.95531 9 GTATCAA 13380 0.0 19.822899 1 ATGGGAG 2360 0.0 19.392586 5 GAGTACT 5455 0.0 18.712841 12-13 AGTACTT 5715 0.0 18.27402 12-13 CATGGGG 3495 0.0 17.5498 4 ATCAACG 15060 0.0 17.419104 3 TCAACGC 15160 0.0 17.304201 4 CAACGCA 15280 0.0 17.137426 5 GTACTTT 6015 0.0 17.127438 14-15 AACGCAG 15375 0.0 17.081133 6 TATCAAC 15415 0.0 17.018385 2 GTGTAGC 1160 0.0 16.680643 1 AGTACAT 5960 0.0 16.545774 2 GGTATCA 10560 0.0 16.49109 1 ATGGGAT 2565 0.0 15.819292 5 >>END_MODULE