FastQCFastQC Report
Fri 27 May 2016
SRR939631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307451
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59950.25981050085137236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37480.16243031813026582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28340.12281950949337601No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24660.10687117516254949No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG91450.027.5054932
GTACATG95100.027.2477071
ACATGGG94650.025.3715923
GTATCAA130500.023.5294421
CATGGGA53350.021.3493774
TATCAAC149700.020.5754872
ATCAACG150300.020.4929053
TCAACGC150700.020.438074
CATGGGG41600.020.4205824
CAACGCA151450.020.367315
AACGCAG155350.019.8843886
GAGTACT56000.019.66048412-13
AGTACTT59400.018.57508312-13
ACGCAGA169000.017.9690997
GGTATCA99800.017.9278661
TACCCCG1600.001200013117.793627
CGCAGAG167850.017.7266378
GTACTTT63250.017.44442614-15
ATGGGAG25900.017.223355
AGTACAT68950.016.8639412
Produced by FastQC (version 0.11.5)