FastQCFastQC Report
Fri 27 May 2016
SRR939631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307451
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61080.26470767959969677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43240.1873929283872117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33520.14526852357861553No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25270.10951478492934412No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24350.10552770134663748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG90700.025.3728181
TACATGG88550.025.0278822
ACATGGG88900.023.9221483
GTATCAA120850.022.8590721
CATGGGA50800.021.5802124
GGTATCA90750.020.7952861
TCAACGC137150.019.8630644
ATCAACG137900.019.7209153
CAACGCA139500.019.5284545
ATGGGAG24700.019.4297285
AACGCAG141150.019.3071126
TATCAAC142300.019.2446422
GAGTACT55050.018.94810912-13
AGTACTT59200.018.85003312-13
CATGGGG38800.018.674654
CGCAGAG153400.017.2449788
ACGCAGA155150.017.2318027
GGACCGA3903.5106495E-1016.8668376
ATGGGAT21550.016.8115185
GTACTTT62750.016.51050414-15
Produced by FastQC (version 0.11.5)