##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939631_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2307451 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54664606095644 31.0 31.0 34.0 30.0 34.0 2 31.753624237307747 31.0 31.0 34.0 30.0 34.0 3 31.80578829192906 31.0 31.0 34.0 30.0 34.0 4 35.59399007822918 37.0 35.0 37.0 33.0 37.0 5 35.3275298153677 37.0 35.0 37.0 33.0 37.0 6 35.339754560335194 37.0 35.0 37.0 32.0 37.0 7 35.26543445559624 37.0 35.0 37.0 32.0 37.0 8 35.23325739094785 37.0 35.0 37.0 32.0 37.0 9 36.840312535347444 39.0 37.0 39.0 32.0 39.0 10-11 36.72561454176058 39.0 35.0 39.0 32.0 39.0 12-13 36.67029657401176 39.0 35.0 39.0 32.0 39.0 14-15 37.74301426119125 39.5 37.0 41.0 32.0 41.0 16-17 37.70485548772216 39.0 37.0 41.0 32.0 41.0 18-19 37.683272797558864 39.0 36.5 41.0 32.0 41.0 20-21 37.611547330799226 39.0 36.5 41.0 32.0 41.0 22-23 37.48203190446948 39.0 36.0 41.0 32.0 41.0 24-25 37.3642248524454 39.0 36.0 41.0 31.5 41.0 26-27 37.10972995742922 39.0 36.0 40.0 31.0 41.0 28-29 36.90598846952763 39.0 36.0 40.0 30.5 41.0 30-31 36.683767932666825 38.5 35.5 40.0 30.0 41.0 32-33 36.53008189556355 38.0 35.0 40.0 30.0 41.0 34-35 36.52676481537419 38.0 35.0 40.0 30.0 41.0 36-37 36.43219704340417 38.0 35.0 40.0 30.0 41.0 38-39 36.47624196570155 39.0 35.0 40.0 30.0 41.0 40-41 36.38405972651207 39.0 35.0 40.0 30.0 41.0 42-43 36.20982114029724 38.5 35.0 40.0 30.0 41.0 44-45 36.019780485046056 38.0 35.0 40.0 29.0 41.0 46-47 35.755668267711854 38.0 35.0 40.0 28.5 41.0 48-49 35.48928297935687 38.0 34.0 40.0 27.5 41.0 50-51 35.25161379374903 38.0 34.0 40.0 27.0 41.0 52-53 35.01870310572142 38.0 34.0 40.0 26.5 41.0 54-55 34.73074227795087 38.0 34.0 40.0 26.0 41.0 56-57 32.648465557881835 37.0 30.5 39.5 16.5 41.0 58-59 27.479641821213107 32.5 17.5 38.5 7.0 40.0 60-61 28.262712620983066 33.0 20.0 38.0 9.5 40.0 62-63 31.460494285685805 34.5 29.5 38.5 15.0 40.0 64-65 31.72353324079255 35.5 31.0 39.0 10.0 40.0 66-67 24.939850293679044 27.0 18.0 35.5 2.0 39.0 68-69 17.319534846027068 13.0 7.5 28.0 2.0 35.0 70-71 16.179182353168063 12.5 7.5 26.5 2.0 33.5 72-73 17.268273085755666 13.0 7.0 28.0 2.0 34.0 74-75 18.31101483845161 17.0 8.5 29.0 2.0 34.5 76-77 19.510052651172224 21.0 8.0 30.0 2.0 34.5 78-79 22.641709401413074 27.5 10.5 33.5 2.0 35.5 80-81 26.40371669864279 31.5 23.5 34.5 2.0 36.0 82-83 27.766231222244805 33.5 27.0 35.0 2.0 36.0 84-85 27.835768993577762 34.0 28.0 35.0 2.0 36.0 86-87 27.59836200205335 34.0 27.0 35.0 2.0 36.0 88-89 27.17803975035656 33.5 26.5 35.0 2.0 35.5 90-91 25.797627555254692 32.5 21.0 35.0 2.0 35.0 92-93 23.277978167250353 31.5 2.0 34.5 2.0 35.0 94-95 15.751643696875904 4.5 2.0 32.0 2.0 34.5 96-97 13.150117597296758 2.0 2.0 30.0 2.0 34.0 98-99 12.786496441311213 2.0 2.0 29.5 2.0 34.0 100-101 12.503532902757197 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3642310042166699 1101 2 0.42752458922176473 1101 3 0.3476585597672859 1101 4 0.3033991726808054 1101 5 0.38737834347917044 1101 6 0.4407664949317933 1101 7 0.3042687331423508 1101 8 0.5063614210898351 1101 9 0.5885065226562034 1101 10-11 0.5461852469226969 1101 12-13 0.5535593855401331 1101 14-15 0.7076349248968867 1101 16-17 0.6823045746299599 1101 18-19 0.5068809620455497 1101 20-21 0.5480340198492399 1101 22-23 0.7171445173958517 1101 24-25 0.6692139219277209 1101 26-27 0.6442610110832021 1101 28-29 0.6763080589098323 1101 30-31 0.5693531255784521 1101 32-33 0.6063481862428617 1101 34-35 0.46212032662084823 1101 36-37 0.2891013838256171 1101 38-39 0.5377223921976864 1101 40-41 0.6171595928658249 1101 42-43 0.7757716360211475 1101 44-45 0.37057296308403664 1101 46-47 0.4417847398374306 1101 48-49 0.409758076366316 1101 50-51 0.3610989793511479 1101 52-53 0.24161063949687644 1101 54-55 0.25012663101151844 1101 56-57 -2.1245253883076103 1101 58-59 -9.869228190826224 1101 60-61 -10.501203939920565 1101 62-63 -3.5919767651263577 1101 64-65 -5.297306925024422 1101 66-67 -9.962497784182784 1101 68-69 -4.892493642381876 1101 70-71 -4.641299187836406 1101 72-73 -4.666138036554035 1101 74-75 -6.802907054635522 1101 76-77 -8.068528944716558 1101 78-79 -11.522758302582755 1101 80-81 -8.27137497612949 1101 82-83 -4.536017880449464 1101 84-85 -3.6685201753129704 1101 86-87 -3.5018319484954468 1101 88-89 -3.8218975472524477 1101 90-91 -10.351284161448685 1101 92-93 -17.272184181229612 1101 94-95 -11.508437423821224 1101 96-97 -9.743935913169906 1101 98-99 -9.607878098874522 1101 100-101 -9.617072781651341 1102 1 0.4157704218778946 1102 2 0.4085849573730336 1102 3 0.41556927950370337 1102 4 0.3669069270764709 1102 5 0.3624311402582805 1102 6 0.3644907011198697 1102 7 0.4548336656796934 1102 8 0.43458860546030564 1102 9 0.4967599423953004 1102 10-11 0.377707014317636 1102 12-13 0.34491439552341063 1102 14-15 0.39331896672786115 1102 16-17 0.41857171133031557 1102 18-19 0.3941644892504357 1102 20-21 0.46928170805239944 1102 22-23 0.4835427229254421 1102 24-25 0.4806275074320965 1102 26-27 0.48514958117560525 1102 28-29 0.5384819620389933 1102 30-31 0.5046038117665645 1102 32-33 0.402726241185114 1102 34-35 0.2565911366728528 1102 36-37 0.3102066937474106 1102 38-39 0.3212655991454554 1102 40-41 0.29114713286596583 1102 42-43 0.271312663489077 1102 44-45 0.37465088668942315 1102 46-47 0.49304892215843665 1102 48-49 0.5048880910602023 1102 50-51 0.5413847280412725 1102 52-53 0.3541002831671278 1102 54-55 0.3381409320477715 1102 56-57 -2.7898710186865543 1102 58-59 -10.27589520687108 1102 60-61 -10.404878971794655 1102 62-63 -4.470890617665024 1102 64-65 -5.780271537680687 1102 66-67 -7.030701341519546 1102 68-69 -4.4798234555298855 1102 70-71 -4.374333709468981 1102 72-73 -4.885289575611649 1102 74-75 -5.667194229108723 1102 76-77 -7.638288701869374 1102 78-79 -10.263920867856136 1102 80-81 -6.07119227267037 1102 82-83 -3.732238675199511 1102 84-85 -3.3297768806739363 1102 86-87 -3.145035935887428 1102 88-89 -3.365760579265757 1102 90-91 -9.283341037551576 1102 92-93 -13.219854062042309 1102 94-95 -10.437702741488014 1102 96-97 -9.35966437270411 1102 98-99 -9.429970455533118 1102 100-101 -9.262452648426235 1103 1 0.19310445448434876 1103 2 0.22214543312145452 1103 3 0.17426904863739168 1103 4 0.20794534765967398 1103 5 0.2288697847302288 1103 6 0.09376734024032629 1103 7 0.05344103519927046 1103 8 0.19215319342006865 1103 9 0.403573266809488 1103 10-11 0.4042724721974267 1103 12-13 0.35268413899181184 1103 14-15 0.44923246998001076 1103 16-17 0.24320641317603275 1103 18-19 0.3652125343194328 1103 20-21 0.2171551631290356 1103 22-23 0.28338897005857433 1103 24-25 0.3761890381576407 1103 26-27 0.46695094134525306 1103 28-29 0.4203094501467959 1103 30-31 0.35639260853140087 1103 32-33 0.46235956268692036 1103 34-35 0.512865254769622 1103 36-37 0.34824436424263894 1103 38-39 0.4346872062298104 1103 40-41 0.2480217195212262 1103 42-43 0.2235914444178846 1103 44-45 0.2092180919935842 1103 46-47 0.08484880718034304 1103 48-49 0.040091885185702836 1103 50-51 0.2436649310276735 1103 52-53 0.17890591633543096 1103 54-55 0.15701061241473013 1103 56-57 -2.9257832250221796 1103 58-59 -10.33331798724775 1103 60-61 -10.619503108700503 1103 62-63 -4.55699772173385 1103 64-65 -5.703768252158824 1103 66-67 -7.1879552193757945 1103 68-69 -4.5073499399304815 1103 70-71 -4.037077488061822 1103 72-73 -4.456477304657726 1103 74-75 -5.376746076190891 1103 76-77 -7.0683748520143865 1103 78-79 -9.310317298074443 1103 80-81 -5.243867227467899 1103 82-83 -3.169629461176431 1103 84-85 -2.8022462249044047 1103 86-87 -2.5776873831249176 1103 88-89 -2.6084307256125463 1103 90-91 -5.475216519155083 1103 92-93 -10.618547153193546 1103 94-95 -8.81412432506101 1103 96-97 -8.338676509112425 1103 98-99 -8.48409102857303 1103 100-101 -8.525213719239394 1104 1 0.17403692124123538 1104 2 0.22938981101132327 1104 3 0.13232829288840975 1104 4 0.1235661185820689 1104 5 0.14699436182246473 1104 6 0.14530057310103217 1104 7 0.14156796391496584 1104 8 0.24447969871538788 1104 9 0.15037374533602588 1104 10-11 0.27465869270922383 1104 12-13 0.2230702794074375 1104 14-15 0.4272738656012507 1104 16-17 0.2920806134959051 1104 18-19 0.27433830708724116 1104 20-21 0.22548476398100803 1104 22-23 0.2530662433800117 1104 24-25 0.2633449930833649 1104 26-27 0.4206000105637173 1104 28-29 0.3386622212178523 1104 30-31 0.3202860575620434 1104 32-33 0.45895050964147543 1104 34-35 0.47658841934993745 1104 36-37 0.20770722973746558 1104 38-39 0.24670422634483913 1104 40-41 0.11278534932571205 1104 42-43 0.3648663447141729 1104 44-45 0.2792936675326061 1104 46-47 0.2986477427343246 1104 48-49 0.288713510253622 1104 50-51 0.245674143368376 1104 52-53 0.1403235127583926 1104 54-55 -9.002616971613975E-4 1104 56-57 -3.226790145971748 1104 58-59 -10.412879031419823 1104 60-61 -10.509114892192233 1104 62-63 -4.376519752665068 1104 64-65 -5.79804880916679 1104 66-67 -6.890365521280675 1104 68-69 -4.284435259648749 1104 70-71 -4.457953929152051 1104 72-73 -4.798885613769977 1104 74-75 -5.452380485888549 1104 76-77 -6.937636922827089 1104 78-79 -9.72810963626737 1104 80-81 -5.382979531264329 1104 82-83 -3.1400893541423223 1104 84-85 -2.51991909165514 1104 86-87 -2.416874384606377 1104 88-89 -2.32915685823642 1104 90-91 -4.7501176965046525 1104 92-93 -7.103737612266325 1104 94-95 -7.918278897206511 1104 96-97 -8.138789636938217 1104 98-99 -8.348431750617545 1104 100-101 -8.46708560830428 1105 1 0.2780513420730699 1105 2 0.2125330458204786 1105 3 0.2798754253238833 1105 4 0.16163783918726438 1105 5 0.18143328783144597 1105 6 0.1307901677868486 1105 7 0.20959312292866628 1105 8 0.08632001994350702 1105 9 0.294486008992493 1105 10-11 0.2912022830868679 1105 12-13 0.3740709763855037 1105 14-15 0.3914737218183646 1105 16-17 0.44074641733231346 1105 18-19 0.34504460478428456 1105 20-21 0.3347852665563664 1105 22-23 0.39044155094610034 1105 24-25 0.3348395872656411 1105 26-27 0.4032045581046333 1105 28-29 0.2759183245952883 1105 30-31 0.3919057887219495 1105 32-33 0.44239644963622027 1105 34-35 0.3040583457600974 1105 36-37 0.30156968475545654 1105 38-39 0.2925915239611214 1105 40-41 0.309148449970742 1105 42-43 0.3900862650585921 1105 44-45 0.2746119990528584 1105 46-47 0.5090796963036865 1105 48-49 0.35578252637119334 1105 50-51 0.2268524949703803 1105 52-53 0.4450749289001692 1105 54-55 0.39592654374026637 1105 56-57 1.5508292302179285 1105 58-59 -8.84841396125504 1105 60-61 -10.054231591979484 1105 62-63 -3.753280852049091 1105 64-65 -4.819802285664803 1105 66-67 -6.279972272096558 1105 68-69 -3.866316306066423 1105 70-71 -4.115624609840706 1105 72-73 -2.370426905815087 1105 74-75 -4.5538408992294475 1105 76-77 -6.556346629301938 1105 78-79 -9.20072649143719 1105 80-81 -4.578423465078995 1105 82-83 -2.1020220374281102 1105 84-85 -1.6444336249939262 1105 86-87 -1.4450936870765787 1105 88-89 -1.4475209008155652 1105 90-91 -4.037767973052389 1105 92-93 -6.570415778904909 1105 94-95 -8.22608171124299 1105 96-97 -8.34761549977679 1105 98-99 -8.682598665730136 1105 100-101 -8.613456154017832 1106 1 0.36943483211579675 1106 2 0.230705432622603 1106 3 0.30639796463060875 1106 4 0.08384795915323906 1106 5 0.11204005560720987 1106 6 0.2971495611322794 1106 7 0.3405619071936812 1106 8 0.23231083085396875 1106 9 0.17408702267100296 1106 10-11 0.30258837686668727 1106 12-13 0.2497926643834063 1106 14-15 0.286696147272238 1106 16-17 0.2951884170670809 1106 18-19 0.34542990935684514 1106 20-21 0.3714619762089555 1106 22-23 0.3895019414743146 1106 24-25 0.4246424390855026 1106 26-27 0.4004546640330702 1106 28-29 0.5181087305518659 1106 30-31 0.5480845296129147 1106 32-33 0.415565626240749 1106 34-35 0.48372914282708734 1106 36-37 0.3915502295025348 1106 38-39 0.41732245765154374 1106 40-41 0.39169985356292614 1106 42-43 0.36444248701989324 1106 44-45 0.45177973147279005 1106 46-47 0.2577183946292081 1106 48-49 0.31191279085266643 1106 50-51 0.38242780766141493 1106 52-53 0.5125751957427909 1106 54-55 0.28701472794900695 1106 56-57 1.5824907136688324 1106 58-59 -8.682086912469739 1106 60-61 -10.065817211264605 1106 62-63 -3.6190284884703594 1106 64-65 -5.319031272212701 1106 66-67 -6.646088232196309 1106 68-69 -3.925563049777832 1106 70-71 -4.142206784430174 1106 72-73 -4.561798347509168 1106 74-75 -5.041276250539887 1106 76-77 -6.736589520776235 1106 78-79 -9.330949531437707 1106 80-81 -4.858678162317741 1106 82-83 -2.254465588084539 1106 84-85 -1.7279826561126264 1106 86-87 -1.5691811661739052 1106 88-89 -1.5353893457861467 1106 90-91 -4.295036402174137 1106 92-93 -6.397196104355874 1106 94-95 -7.2819397558202645 1106 96-97 -7.173405070920266 1106 98-99 -6.7780984053564275 1106 100-101 -7.3755540302740155 1107 1 0.059182862864588515 1107 2 -0.01925739677222893 1107 3 -0.03701730220688759 1107 4 -0.016837719882545343 1107 5 -0.07887529031667384 1107 6 0.016625376512998002 1107 7 -0.046363530154074795 1107 8 0.15896057303232425 1107 9 0.13081490220660896 1107 10-11 0.03995590715677366 1107 12-13 -0.014111514541646386 1107 14-15 0.171839372731732 1107 16-17 0.12443100667196916 1107 18-19 0.14549317847202303 1107 20-21 0.10496297269751409 1107 22-23 0.1649448252805925 1107 24-25 0.2576139521663947 1107 26-27 0.2348576408449361 1107 28-29 0.06349562119120122 1107 30-31 0.12170882178968867 1107 32-33 0.28540326187391685 1107 34-35 0.13141982012296438 1107 36-37 0.2265696847554537 1107 38-39 0.14869009866257699 1107 40-41 0.22656983553122956 1107 42-43 0.01714210107829217 1107 44-45 -0.012759511195902462 1107 46-47 0.08988124933168251 1107 48-49 -0.029452556652948658 1107 50-51 0.11147782189696187 1107 52-53 -0.02285788135062461 1107 54-55 -0.19342325250084258 1107 56-57 0.9412459729140323 1107 58-59 -9.028329517891901 1107 60-61 -10.629130219394685 1107 62-63 -4.237405398406771 1107 64-65 -5.827943517949944 1107 66-67 -7.239889045204439 1107 68-69 -4.111321171950493 1107 70-71 -4.1080505571675126 1107 72-73 -2.6465931974031918 1107 74-75 -4.750337969561748 1107 76-77 -6.858425274153223 1107 78-79 -7.847158683315218 1107 80-81 -2.833329391320305 1107 82-83 -1.7076923129587946 1107 84-85 -1.4566044175247903 1107 86-87 -1.4491881257765762 1107 88-89 -1.235154459196039 1107 90-91 -0.8586309578667759 1107 92-93 -3.6043107163580252 1107 94-95 -6.053501474842125 1107 96-97 -6.73534434838697 1107 98-99 -6.207808000117744 1107 100-101 -6.817215556325073 1108 1 -0.028058973274895038 1108 2 -0.09998083230005861 1108 3 -0.1940389307936634 1108 4 -0.06672501263467723 1108 5 -0.04714622272199165 1108 6 -0.13717338502548415 1108 7 -0.12805535821949832 1108 8 -0.08090632036704903 1108 9 -0.14557078726844708 1108 10-11 -0.11848733222089436 1108 12-13 -0.14226814136531374 1108 14-15 -0.22088219422857236 1108 16-17 -0.12276820174087533 1108 18-19 -0.1375830208550255 1108 20-21 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2.68881130824143 2316 92-93 4.723530483667027 2316 94-95 11.856585149019132 2316 96-97 13.6958470566399 2316 98-99 13.50521265478296 2316 100-101 12.295687125828163 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 19.0 4 30.0 5 69.0 6 164.0 7 414.0 8 1099.0 9 1884.0 10 2999.0 11 4838.0 12 7322.0 13 10229.0 14 12636.0 15 17450.0 16 24303.0 17 32078.0 18 36512.0 19 37827.0 20 39702.0 21 39955.0 22 39993.0 23 34239.0 24 34431.0 25 41183.0 26 58114.0 27 87098.0 28 123061.0 29 166865.0 30 207683.0 31 226160.0 32 224963.0 33 204708.0 34 181112.0 35 161148.0 36 130192.0 37 83360.0 38 31421.0 39 2190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.11359276959589 20.01122706633289 14.447413765076888 23.427766398994333 2 15.352339212335373 21.38397599567624 39.83437432685044 23.429310465137952 3 18.889171548320245 25.822889259957645 30.395731389946214 24.8922078017759 4 12.841312773272326 17.407476908502066 38.01489175718141 31.736318561044204 5 13.12615387820254 38.39112814677287 34.79640966484476 13.68630831017982 6 30.924296983987958 37.43061066085477 17.904865585444718 13.740226769712551 7 27.38992073937865 33.09132024905404 21.857712254778107 17.6610467567892 8 25.439419541921083 35.623525679557986 20.386457769481833 18.550597009039105 9 26.164065889156475 16.611056962856416 20.58596260548978 36.638914542497325 10-11 24.383963083073056 26.72511788982735 28.43551174001095 20.455407287088654 12-13 25.53020627523618 24.220774352304772 28.36692956860189 21.88208980385716 14-15 22.54590478881565 25.49471513656482 26.693403379244263 25.265976695375265 16-17 21.901960214973144 28.62166953924482 27.2020294255436 22.274340820238436 18-19 21.79238708737965 27.760712267405374 29.063970238982634 21.38293040623234 20-21 22.854114778602018 26.81201030921133 28.683686024101917 21.65018888808473 22-23 22.339396253382805 26.86159107392322 28.435975516605726 22.363037156088247 24-25 22.274225286403883 26.989908121499518 28.68166778947925 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22.485909386633935 27.252388424317882 27.908753611190836 22.35294857785735 52-53 22.564728209299172 27.348244543852513 27.67991324386607 22.407114002982244 54-55 22.386613520933725 27.15081908909139 27.826605540399285 22.6359618495756 56-57 21.48783224416066 27.34151055579191 28.72286076955803 22.447796430489394 58-59 21.006105828038084 26.784273445232028 29.842496113782204 22.367124612947688 60-61 22.196380492323808 27.156394923991904 28.332693523571074 22.314531060113215 62-63 22.513728438155447 27.1354246687373 27.861605468275126 22.48924142483213 64-65 21.99476594051432 27.151000142762854 28.246195196311945 22.60803872041088 66-67 21.629796189299384 26.967684968883493 28.93114947679542 22.471369365021705 68-69 21.76678790714411 26.6284536682648 29.38520497111256 22.219553453478525 70-71 21.71556923815188 26.576860134029683 29.219188904787995 22.488381723030447 72-73 20.93183783730825 26.65188301214252 29.68760159216352 22.728677558385712 74-75 21.477969209232896 26.922918667759777 28.91599634195387 22.683115781053463 76-77 19.833644859813084 27.287872457394176 29.577130291368885 23.301352391423862 78-79 20.709418826274963 27.290959259704152 28.89117315834604 23.108448755674846 80-81 21.83511308909128 27.373238338829104 27.603858954798838 23.187789617280785 82-83 22.13898443105157 27.311847668774842 27.394384829457515 23.15478307071608 84-85 22.18650695194582 27.16334824519301 27.261270299757633 23.388874503103537 86-87 22.06917652840621 27.107867599849094 27.447399059869042 23.375556811875647 88-89 22.22779355036666 27.09487837000948 27.337852320896367 23.339475758727495 90-91 21.47974585504526 27.146669968943392 27.937521793396968 23.43606238261438 92-93 20.41055848643765 27.369695418902907 28.66024091981394 23.5595051748455 94-95 19.492150115680875 27.407894439928903 29.15275317829279 23.947202266097428 96-97 19.482384839502465 27.4714700917516 28.765452450597255 24.280692618148677 98-99 19.85931328625741 27.33231897082234 28.398961180179548 24.409406562740696 100-101 20.386244212879387 27.327090967256023 28.066883994133995 24.21978082573059 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 321.0 1 249.5 2 170.0 3 180.0 4 205.0 5 253.5 6 342.5 7 485.0 8 663.5 9 789.5 10 843.5 11 949.5 12 1151.0 13 1329.0 14 1471.5 15 1657.5 16 1871.5 17 2102.0 18 2345.5 19 2660.5 20 3138.5 21 3666.0 22 4380.0 23 5433.5 24 6900.5 25 8847.5 26 11433.5 27 14726.0 28 18597.5 29 22946.0 30 27423.5 31 32517.0 32 38552.0 33 44889.0 34 51178.0 35 56900.0 36 62446.5 37 67968.5 38 72071.5 39 74984.0 40 77900.0 41 80588.0 42 82949.5 43 85871.0 44 88506.5 45 90217.0 46 92495.5 47 94618.5 48 95838.5 49 93952.5 50 89264.5 51 84388.0 52 79600.0 53 75259.0 54 70450.0 55 64479.5 56 57905.5 57 51435.5 58 44847.5 59 38153.5 60 31172.5 61 24503.5 62 18871.0 63 13947.0 64 9952.0 65 6974.5 66 4747.0 67 3257.0 68 2211.0 69 1432.5 70 935.0 71 601.5 72 388.0 73 281.5 74 197.5 75 140.0 76 86.5 77 50.0 78 36.0 79 26.0 80 13.5 81 9.5 82 8.0 83 4.0 84 2.5 85 1.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022795717005474874 2 0.008667573005883982 3 8.667573005883982E-5 4 0.0 5 0.14149812932105602 6 0.0 7 0.0 8 0.001386811680941437 9 0.0 10-11 0.0 12-13 0.0 14-15 5.200543803530389E-4 16-17 0.0 18-19 1.3001359508825973E-4 20-21 0.0 22-23 4.117097177794891E-4 24-25 6.500679754412987E-5 26-27 4.333786502941991E-4 28-29 0.003835401055103662 30-31 0.013239717766487781 32-33 0.003272008809721203 34-35 0.005157205938500969 36-37 0.11155166458572685 38-39 0.11155166458572685 40-41 0.1234262396037879 42-43 0.12526809886753823 44-45 0.12199609005781704 46-47 0.11543040350585994 48-49 0.11614547827884536 50-51 0.11426028115006559 52-53 0.09267802436541447 54-55 0.07978500951916205 56-57 0.5787771874679029 58-59 1.6282252580878207 60-61 0.5416366371376901 62-63 0.004572144760603801 64-65 0.12697994453620035 66-67 1.9356857415390405 68-69 2.927472782737315 70-71 2.825043738740281 72-73 2.6849107521676516 74-75 2.0467606896094432 76-77 1.4605293893564804 78-79 0.9645491930272843 80-81 0.055580811900231036 82-83 2.3835825766180952E-4 84-85 1.5168252760296968E-4 86-87 2.816961226912294E-4 88-89 8.667573005883982E-5 90-91 0.2603955620292695 92-93 0.4120564206997245 94-95 2.0522212606031505 96-97 2.466704601744522 98-99 1.4779078732332778 100-101 0.9716349339595944 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2307451.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.46159686664287 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0063421392869 38.495740352921146 2 14.864686911350777 15.893797984069957 3 5.490151736513236 8.8053683662251 4 2.6041159099494964 5.5688077988692335 5 1.4578981459423035 3.8970781475496747 6 0.8854998035048809 2.840414011228164 7 0.5737128012659685 2.1470121748959525 8 0.39904446246074016 1.7066843347153835 9 0.3008364080562204 1.4474875293279477 >10 1.316946919041329 12.109657221809716 >50 0.0668454974686368 2.432594899803141 >100 0.03164425326330134 3.0078927016276675 >500 0.0013812572226547875 0.5004900388510335 >1k 8.125042486198895E-4 0.8816565108968909 >5k 8.125042486198894E-5 0.2653179272088589 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6108 0.26470767959969677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4324 0.1873929283872117 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3352 0.14526852357861553 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2527 0.10951478492934412 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2435 0.10552770134663748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.667573005883982E-5 2 0.0 0.0 0.0 0.0 8.667573005883982E-5 3 0.0 0.0 0.0 0.0 8.667573005883982E-5 4 0.0 0.0 0.0 0.0 8.667573005883982E-5 5 0.0 0.0 0.0 0.0 8.667573005883982E-5 6 0.0 0.0 0.0 0.0 1.3001359508825973E-4 7 0.0 0.0 0.0 0.0 1.3001359508825973E-4 8 0.0 0.0 0.0 0.0 1.3001359508825973E-4 9 0.0 0.0 0.0 0.0 1.3001359508825973E-4 10-11 0.0 0.0 0.0 2.1668932514709954E-5 1.3001359508825973E-4 12-13 0.0 0.0 0.0 4.333786502941991E-5 1.3001359508825973E-4 14-15 0.0 0.0 0.0 4.333786502941991E-5 1.9502039263238959E-4 16-17 0.0 0.0 0.0 8.667573005883982E-5 2.6002719017651947E-4 18-19 0.0 0.0 0.0 1.3001359508825973E-4 3.0336505520593937E-4 20-21 0.0 0.0 0.0 2.3835825766180952E-4 3.0336505520593937E-4 22-23 0.0 0.0 0.0 6.500679754412986E-4 3.4670292023535927E-4 24-25 0.0 0.0 0.0 0.001061777693220788 3.4670292023535927E-4 26-27 0.0 0.0 0.0 0.0019068660612944761 3.4670292023535927E-4 28-29 0.0 0.0 0.0 0.004247110772883151 3.4670292023535927E-4 30-31 0.0 0.0 0.0 0.012026257545664025 3.4670292023535927E-4 32-33 0.0 0.0 0.0 0.028646328784446562 4.7671651532361903E-4 34-35 0.0 0.0 0.0 0.052612168145715774 4.7671651532361903E-4 36-37 0.0 0.0 0.0 0.08910265050048734 4.7671651532361903E-4 38-39 0.0 0.0 0.0 0.14492182065838016 4.98385447838329E-4 40-41 0.0 0.0 0.0 0.20886684050928925 5.633922453824588E-4 42-43 0.0 0.0 0.0 0.2742203409736545 5.633922453824588E-4 44-45 0.0 0.0 0.0 0.35285689706953693 5.850611778971688E-4 46-47 0.0 0.0 0.0 0.4440831029564658 6.067301104118787E-4 48-49 0.0 0.0 0.0 0.5283969193712023 6.067301104118787E-4 50-51 0.0 0.0 0.0 0.6122773571356445 6.067301104118787E-4 52-53 0.0 0.0 0.0 0.6914122986793652 6.067301104118787E-4 54-55 0.0 0.0 0.0 0.7781963734007786 6.067301104118787E-4 56-57 0.0 0.0 0.0 0.8502455740121893 6.283990429265887E-4 58-59 0.0 4.333786502941991E-5 0.0 0.9007558557039781 6.500679754412986E-4 60-61 0.0 4.333786502941991E-5 0.0 0.9413634352365445 6.717369079560086E-4 62-63 4.333786502941991E-5 6.500679754412987E-5 2.1668932514709954E-5 0.9780056001189191 6.934058404707185E-4 64-65 4.333786502941991E-5 8.667573005883982E-5 4.333786502941991E-5 1.0104006542284105 6.934058404707185E-4 66-67 4.333786502941991E-5 8.667573005883982E-5 4.333786502941991E-5 1.0404988014913426 7.367437055001384E-4 68-69 4.333786502941991E-5 8.667573005883982E-5 4.333786502941991E-5 1.0815180907416886 7.367437055001384E-4 70-71 4.333786502941991E-5 8.667573005883982E-5 4.333786502941991E-5 1.1486701126047747 7.367437055001384E-4 72-73 4.333786502941991E-5 8.667573005883982E-5 4.333786502941991E-5 1.2437967263443515 7.800815705295583E-4 74-75 4.333786502941991E-5 8.667573005883982E-5 4.333786502941991E-5 1.3696498863897868 7.800815705295583E-4 76-77 4.333786502941991E-5 8.667573005883982E-5 4.333786502941991E-5 1.5724710947274718 7.800815705295583E-4 78-79 4.333786502941991E-5 1.0834466257354978E-4 4.333786502941991E-5 1.880278281098927 7.800815705295583E-4 80-81 4.333786502941991E-5 1.3001359508825973E-4 4.333786502941991E-5 2.2579677748303215 8.234194355589782E-4 82-83 4.333786502941991E-5 1.3001359508825973E-4 4.333786502941991E-5 2.6396226832119076 9.534330306472381E-4 84-85 4.333786502941991E-5 1.3001359508825973E-4 4.333786502941991E-5 2.929878034246448 9.534330306472381E-4 86-87 4.333786502941991E-5 1.3001359508825973E-4 4.333786502941991E-5 3.153003032350416 9.534330306472381E-4 88-89 4.333786502941991E-5 1.3001359508825973E-4 4.333786502941991E-5 3.363906752516088 9.534330306472381E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9070 0.0 25.372818 1 TACATGG 8855 0.0 25.027882 2 ACATGGG 8890 0.0 23.922148 3 GTATCAA 12085 0.0 22.859072 1 CATGGGA 5080 0.0 21.580212 4 GGTATCA 9075 0.0 20.795286 1 TCAACGC 13715 0.0 19.863064 4 ATCAACG 13790 0.0 19.720915 3 CAACGCA 13950 0.0 19.528454 5 ATGGGAG 2470 0.0 19.429728 5 AACGCAG 14115 0.0 19.307112 6 TATCAAC 14230 0.0 19.244642 2 GAGTACT 5505 0.0 18.948109 12-13 AGTACTT 5920 0.0 18.850033 12-13 CATGGGG 3880 0.0 18.67465 4 CGCAGAG 15340 0.0 17.244978 8 ACGCAGA 15515 0.0 17.231802 7 GGACCGA 390 3.5106495E-10 16.866837 6 ATGGGAT 2155 0.0 16.811518 5 GTACTTT 6275 0.0 16.510504 14-15 >>END_MODULE