FastQCFastQC Report
Fri 27 May 2016
SRR939630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316878
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.11057984063036552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG77900.031.7332671
GTATCAA110900.030.5124661
TACATGG90850.026.8798432
ACATGGG92800.024.6358663
GTATAGG5502.1827873E-1123.9465581
TATCAAC136300.023.5653652
ATCAACG148600.021.6470553
CATGGGA54850.021.5271684
TCAACGC149000.021.4598144
CAACGCA150400.021.1812155
AACGCAG153700.020.7878576
GAGTACT55400.020.57366812-13
AGTACTT61550.019.74483512-13
ACGCAGA160100.019.5146877
CATGGGG38750.018.7703574
GTGTAGC13000.018.703821
GGTATCA84750.018.409561
CGCAGAG167100.018.3582738
GTACTTT63350.018.06923914-15
ATGGGAG27450.018.0526875
Produced by FastQC (version 0.11.5)