FastQCFastQC Report
Fri 27 May 2016
SRR939630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316878
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63520.2741620404699773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43360.18714839538378802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.14415951120430165No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24820.10712691820631039No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23490.10138643467631873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG92500.025.2448182
GTACATG96050.024.7596861
ACATGGG92450.024.1343083
GTATCAA122000.024.0570561
CATGGGG40600.020.797334
TCAACGC140450.020.5210744
ATCAACG141150.020.3858853
GGTATCA90950.020.2855131
TATCAAC143800.020.0771142
CAACGCA143950.020.0221235
AACGCAG144400.019.914596
CATGGGA51900.019.3594424
GAGTACT59500.018.93687812-13
AGTACTT62300.018.16145512-13
ACGCAGA158800.017.8118697
CGCAGAG158550.017.7507538
GTACTTT64700.017.41490614-15
GTCGGGA3301.319313E-817.154422
AGAGTAC125700.016.5207110-11
AGTACAT69750.016.4350412
Produced by FastQC (version 0.11.5)