##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939629_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1163154 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45507559618073 31.0 31.0 34.0 30.0 34.0 2 31.615117172790534 31.0 31.0 34.0 30.0 34.0 3 31.677746884763323 31.0 31.0 34.0 30.0 34.0 4 35.3823268458003 37.0 35.0 37.0 33.0 37.0 5 35.15446363938051 37.0 35.0 37.0 32.0 37.0 6 35.22297047510476 37.0 35.0 37.0 32.0 37.0 7 35.17589072470197 37.0 35.0 37.0 32.0 37.0 8 35.17883616442879 37.0 35.0 37.0 32.0 37.0 9 36.716541403803795 39.0 35.0 39.0 32.0 39.0 10-11 36.592136122989736 38.5 35.0 39.0 32.0 39.0 12-13 36.544238768039314 38.0 35.0 39.0 32.0 39.0 14-15 37.3218834307409 39.0 36.0 41.0 32.0 41.0 16-17 37.31917183795095 39.0 36.0 40.0 32.0 41.0 18-19 37.488428015550824 39.0 36.0 41.0 32.0 41.0 20-21 37.44133407958017 39.0 36.0 41.0 32.0 41.0 22-23 37.33460143712698 39.0 36.0 40.5 31.5 41.0 24-25 37.164180753365414 39.0 36.0 40.0 31.0 41.0 26-27 36.62055712313244 38.5 35.5 40.0 30.5 41.0 28-29 36.371383754859636 38.0 35.0 40.0 30.0 41.0 30-31 36.29400320163968 38.0 35.0 40.0 30.0 41.0 32-33 36.22829092278408 38.0 35.0 40.0 30.0 41.0 34-35 36.28839732314036 38.0 35.0 40.0 30.0 41.0 36-37 36.073148095609014 38.0 35.0 40.0 30.0 41.0 38-39 35.95106623886433 38.0 35.0 40.0 29.5 41.0 40-41 35.772019870111784 38.0 35.0 40.0 29.0 41.0 42-43 35.76096974261362 38.0 35.0 40.0 29.0 41.0 44-45 35.64375181618255 38.0 35.0 40.0 28.5 41.0 46-47 35.28192698473289 38.0 34.0 40.0 27.0 41.0 48-49 34.977659020215725 38.0 34.0 40.0 26.0 41.0 50-51 34.73535404598187 38.0 33.0 40.0 26.0 41.0 52-53 34.15634000312942 38.0 33.0 40.0 23.5 41.0 54-55 33.923546667079336 37.0 33.0 40.0 23.0 41.0 56-57 32.04556232450733 36.0 30.0 39.5 15.5 40.5 58-59 27.0611918112305 32.5 17.0 38.0 6.0 40.0 60-61 27.722230676247513 32.0 18.5 38.0 7.0 40.0 62-63 30.694972892669412 34.5 29.0 38.0 4.5 40.0 64-65 30.624324036198132 35.0 29.5 38.0 2.0 40.0 66-67 24.022114440564188 26.5 16.5 34.5 2.0 38.5 68-69 16.89732872861203 13.0 7.0 28.0 2.0 34.5 70-71 15.81802065762573 12.5 7.0 26.0 2.0 33.5 72-73 16.84606681488436 13.0 7.0 28.0 2.0 34.0 74-75 17.733260170192423 17.0 7.5 29.0 2.0 34.0 76-77 18.664595143893244 20.5 7.0 29.5 2.0 33.5 78-79 21.557977275580015 26.5 9.0 33.0 2.0 35.0 80-81 25.219419784482536 30.5 21.0 34.0 2.0 36.0 82-83 26.59651172587637 32.5 25.5 35.0 2.0 36.0 84-85 26.705188220992234 33.0 26.0 35.0 2.0 35.5 86-87 26.389876576962294 33.0 25.0 35.0 2.0 35.0 88-89 25.775113183636904 32.5 22.0 35.0 2.0 35.0 90-91 24.317203053078096 31.5 10.5 34.0 2.0 35.0 92-93 22.02800446028643 30.5 2.0 34.0 2.0 35.0 94-95 15.187240898453688 4.5 2.0 31.5 2.0 34.0 96-97 12.661723211199892 2.0 2.0 28.5 2.0 34.0 98-99 12.283236355633047 2.0 2.0 29.0 2.0 34.0 100-101 11.960110183174368 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4529452498720836 1101 2 0.44667051984333384 1101 3 0.41741541672935867 1101 4 0.24477672307507703 1101 5 0.39530895473166794 1101 6 0.40968207819761915 1101 7 0.4066412516850093 1101 8 0.5847111947674435 1101 9 0.41339414136030683 1101 10-11 0.5894542874402902 1101 12-13 0.5394118933058323 1101 14-15 0.7910537244431239 1101 16-17 0.7283409934153582 1101 18-19 0.5818019910145367 1101 20-21 0.519896707323305 1101 22-23 0.6705433510862662 1101 24-25 0.645450390868767 1101 26-27 0.6761085615982196 1101 28-29 0.6812451295120212 1101 30-31 0.6317246763364821 1101 32-33 0.7036164341497866 1101 34-35 0.4753536603313222 1101 36-37 0.384423213149077 1101 38-39 0.35924720066346794 1101 40-41 0.49977238029865134 1101 42-43 0.3521393772874859 1101 44-45 0.2495624820525748 1101 46-47 0.5129394974964185 1101 48-49 0.42154098825141517 1101 50-51 0.23564709966567676 1101 52-53 0.3207465249541883 1101 54-55 0.12705571555500228 1101 56-57 -1.9984787438748626 1101 58-59 -9.497513582741778 1101 60-61 -10.200366583577402 1101 62-63 -4.183364935330122 1101 64-65 -5.087137790284341 1101 66-67 -9.210775700978267 1101 68-69 -4.571545432787902 1101 70-71 -3.944472306228409 1101 72-73 -4.137550293756185 1101 74-75 -6.1110464265177065 1101 76-77 -7.115522126483516 1101 78-79 -10.266885665412472 1101 80-81 -7.154854008497686 1101 82-83 -3.379347011953154 1101 84-85 -2.577314518084737 1101 86-87 -2.408565076075888 1101 88-89 -2.5569237384590515 1101 90-91 -8.690541552267527 1101 92-93 -15.815936785953493 1101 94-95 -10.108230680412927 1101 96-97 -8.39139193024171 1101 98-99 -8.453428269946437 1101 100-101 -8.333632607856195 1102 1 0.40356419188906045 1102 2 0.3690800206606184 1102 3 0.37467991903708153 1102 4 0.31368827611344585 1102 5 0.38610098431771434 1102 6 0.30040417175793976 1102 7 0.48078326603909716 1102 8 0.4696074699779871 1102 9 0.40814156912311006 1102 10-11 0.35819943532140996 1102 12-13 0.26100959573540905 1102 14-15 0.3523439704148359 1102 16-17 0.4611538465759182 1102 18-19 0.47032486681012386 1102 20-21 0.5621076848225712 1102 22-23 0.6296811397975759 1102 24-25 0.45039070775921886 1102 26-27 0.45714779128647365 1102 28-29 0.594206306009589 1102 30-31 0.5368831543189145 1102 32-33 0.40443887853673033 1102 34-35 0.2362293674713456 1102 36-37 0.30639991315137394 1102 38-39 0.23409856613570668 1102 40-41 0.2559777694672434 1102 42-43 0.47806107514420404 1102 44-45 0.5605005911265764 1102 46-47 0.40579120036493777 1102 48-49 0.5418080226691089 1102 50-51 0.37183809216762853 1102 52-53 0.16349487973783994 1102 54-55 0.04320536882299564 1102 56-57 -2.7753431040175514 1102 58-59 -10.249191556005577 1102 60-61 -10.649465604473043 1102 62-63 -4.355394046405561 1102 64-65 -5.093493451826731 1102 66-67 -6.032689425800477 1102 68-69 -3.8068478119230527 1102 70-71 -3.8921844809931336 1102 72-73 -4.100616456184653 1102 74-75 -4.925775739703349 1102 76-77 -6.604620213806914 1102 78-79 -9.23858900891137 1102 80-81 -5.073407200823397 1102 82-83 -2.8101729940875337 1102 84-85 -2.4358775047297385 1102 86-87 -2.2652318464728047 1102 88-89 -2.479782009090176 1102 90-91 -8.836899474037448 1102 92-93 -13.313285932144355 1102 94-95 -10.336568119280322 1102 96-97 -8.847710167002182 1102 98-99 -8.802626462419552 1102 100-101 -8.65235335183138 1103 1 0.20101385595626198 1103 2 0.22596711538853853 1103 3 0.2785368702411226 1103 4 0.13420447080060427 1103 5 0.24369463115233714 1103 6 0.2695284520465506 1103 7 0.2755119959188903 1103 8 0.2164613898237988 1103 9 0.4297322358869522 1103 10-11 0.34250345112874214 1103 12-13 0.33408375198937534 1103 14-15 0.42389165019759645 1103 16-17 0.40587508578867215 1103 18-19 0.6038095656043296 1103 20-21 0.36458520607312295 1103 22-23 0.3939427624785097 1103 24-25 0.4523406431100341 1103 26-27 0.31745663621211406 1103 28-29 0.49887474614229177 1103 30-31 0.4411777359347937 1103 32-33 0.5428417738482452 1103 34-35 0.6048548773090872 1103 36-37 0.3642970332260873 1103 38-39 0.6161974915286592 1103 40-41 0.5206321460413008 1103 42-43 0.2985192340327174 1103 44-45 0.4634482023461075 1103 46-47 0.46503191666166543 1103 48-49 0.19561908510213044 1103 50-51 0.4784410814161575 1103 52-53 0.1716290018181681 1103 54-55 -0.15650998582972164 1103 56-57 -2.664999167678772 1103 58-59 -9.794275923860763 1103 60-61 -10.261195314213069 1103 62-63 -4.282063452901795 1103 64-65 -5.692898652374161 1103 66-67 -6.426871269503323 1103 68-69 -4.020169524599961 1103 70-71 -4.062715589530875 1103 72-73 -4.069346970327841 1103 74-75 -4.874926257571627 1103 76-77 -6.279917681016688 1103 78-79 -8.764119699051493 1103 80-81 -4.916124787829855 1103 82-83 -2.7414265538414604 1103 84-85 -2.4010728964173964 1103 86-87 -2.067984025514594 1103 88-89 -2.087842641653392 1103 90-91 -4.564804705627278 1103 92-93 -9.336367309131406 1103 94-95 -7.943798367538574 1103 96-97 -7.663027771284414 1103 98-99 -7.709181740842921 1103 100-101 -7.79023088819823 1104 1 0.19231379421388795 1104 2 0.26985839461697125 1104 3 0.28937759233656735 1104 4 0.2514511093251315 1104 5 0.29898405578695986 1104 6 0.12737002382544915 1104 7 0.3319123961797956 1104 8 0.17515206440278064 1104 9 0.05825024472443374 1104 10-11 0.35921066647027544 1104 12-13 0.39124286731212976 1104 14-15 0.5206858855115186 1104 16-17 0.5707969013628542 1104 18-19 0.3363634740514172 1104 20-21 0.5855648388318073 1104 22-23 0.4288949460101321 1104 24-25 0.4916670512329304 1104 26-27 0.6757124044788583 1104 28-29 0.5664449030917922 1104 30-31 0.4932000406092101 1104 32-33 0.6434005520073143 1104 34-35 0.44846860618071105 1104 36-37 0.3870033233997674 1104 38-39 0.3129030810522053 1104 40-41 0.29853669891232215 1104 42-43 0.6487639777050944 1104 44-45 0.37671952233612416 1104 46-47 0.41172057058114575 1104 48-49 0.27009896850776016 1104 50-51 0.3002853009557711 1104 52-53 0.4757343857918599 1104 54-55 0.3767505727935969 1104 56-57 -2.905204098165374 1104 58-59 -9.989766988636063 1104 60-61 -9.970907447654014 1104 62-63 -4.0435762765411845 1104 64-65 -5.2525748823345 1104 66-67 -6.254772628803291 1104 68-69 -3.900685450842019 1104 70-71 -4.015394608543268 1104 72-73 -4.188390718380248 1104 74-75 -4.918894956704332 1104 76-77 -6.392048799366561 1104 78-79 -8.716165810346476 1104 80-81 -4.574578170402248 1104 82-83 -2.5357092874608576 1104 84-85 -1.8904857245084337 1104 86-87 -1.8635354778152227 1104 88-89 -1.8124329451974823 1104 90-91 -4.117160883638864 1104 92-93 -6.357815525860687 1104 94-95 -7.652040490547188 1104 96-97 -7.413598259204006 1104 98-99 -7.577443063987795 1104 100-101 -7.8791331320206055 1105 1 0.32556580760729403 1105 2 0.3874395152110175 1105 3 0.3121583784249289 1105 4 0.19084337127814166 1105 5 0.22759516525058388 1105 6 0.33272198298359257 1105 7 0.36025509712209214 1105 8 0.29818900171196105 1105 9 0.4682609006679357 1105 10-11 0.3957173226457371 1105 12-13 0.4954496914653319 1105 14-15 0.5527505882423256 1105 16-17 0.6105846919053519 1105 18-19 0.6143860683420215 1105 20-21 0.5152046389455478 1105 22-23 0.5897482913635272 1105 24-25 0.663241251065152 1105 26-27 0.7078367462255812 1105 28-29 0.7871553915847542 1105 30-31 0.8239698847503902 1105 32-33 0.7831018165521542 1105 34-35 0.8316080962374741 1105 36-37 0.6718303669586447 1105 38-39 0.6396210965820686 1105 40-41 0.5636986830924897 1105 42-43 0.3943518437683835 1105 44-45 0.729863223368703 1105 46-47 0.641316942947391 1105 48-49 0.47711587765150654 1105 50-51 0.6586894267894863 1105 52-53 0.7880530387013565 1105 54-55 0.6421412281176515 1105 56-57 1.9541891786393961 1105 58-59 -8.307429099672706 1105 60-61 -9.449976789048172 1105 62-63 -3.306935251356389 1105 64-65 -4.36189912944959 1105 66-67 -5.746854808072243 1105 68-69 -3.548909493207466 1105 70-71 -3.6316330275360205 1105 72-73 -1.7848583093528099 1105 74-75 -3.776126731054017 1105 76-77 -5.798111088858956 1105 78-79 -8.289246669649142 1105 80-81 -3.6818671125379048 1105 82-83 -1.3895551472940149 1105 84-85 -0.9348980100139102 1105 86-87 -0.7468720072726143 1105 88-89 -1.0407126462511478 1105 90-91 -3.638455636789356 1105 92-93 -5.79716528265871 1105 94-95 -7.361407374616191 1105 96-97 -7.287989595269531 1105 98-99 -8.00637411535445 1105 100-101 -7.786340095221685 1106 1 0.21800040583444869 1106 2 0.19035805217820112 1106 3 0.21835639749799896 1106 4 0.12022466672184606 1106 5 0.04061349865221331 1106 6 0.12067209657331546 1106 7 0.16817144285989372 1106 8 0.08189625624391539 1106 9 0.01058357603747595 1106 10-11 0.12017560976416064 1106 12-13 0.20348035617529092 1106 14-15 0.2032613079031833 1106 16-17 0.18820491261181616 1106 18-19 0.29602405224429873 1106 20-21 0.19011405539789195 1106 22-23 0.3493127819183215 1106 24-25 0.4621509842213314 1106 26-27 0.357104611690346 1106 28-29 0.5156134536767638 1106 30-31 0.5339778648536111 1106 32-33 0.3957237145387893 1106 34-35 0.6327430905527791 1106 36-37 0.5480172249996258 1106 38-39 0.5351744636091738 1106 40-41 0.5454673767794631 1106 42-43 0.5617794793728379 1106 44-45 0.37736803469724833 1106 46-47 0.4150466376129458 1106 48-49 0.477347154794451 1106 50-51 0.7288896794580282 1106 52-53 0.4479092996097478 1106 54-55 0.21082602515285487 1106 56-57 1.6539990868855767 1106 58-59 -8.529667334576665 1106 60-61 -9.85385231332845 1106 62-63 -3.5831072465419354 1106 64-65 -5.088008304762525 1106 66-67 -6.344124904354377 1106 68-69 -3.6404080788053594 1106 70-71 -3.89017448135942 1106 72-73 -4.4175613835330445 1106 74-75 -4.887405068581131 1106 76-77 -6.036983567642926 1106 78-79 -8.497866986894707 1106 80-81 -4.256218199575205 1106 82-83 -2.1242066445877974 1106 84-85 -1.6365907781235052 1106 86-87 -1.4651889486358698 1106 88-89 -1.5357471329374306 1106 90-91 -3.4432184874294656 1106 92-93 -5.783625846433209 1106 94-95 -6.854598242155506 1106 96-97 -6.701492291072024 1106 98-99 -6.265617040392182 1106 100-101 -6.468462510977423 1107 1 0.11848319229997628 1107 2 0.05136175173599611 1107 3 0.06822920591958237 1107 4 0.17348061346795873 1107 5 0.02077147532244794 1107 6 0.13272359288232138 1107 7 0.12623283893056225 1107 8 0.1545397503484125 1107 9 0.31454642828206403 1107 10-11 0.2096713882102108 1107 12-13 0.1288314782785207 1107 14-15 0.2086152816203466 1107 16-17 0.13796269679810536 1107 18-19 0.17695511790477525 1107 20-21 0.06390340475693534 1107 22-23 0.2745874725422013 1107 24-25 0.3535493923901143 1107 26-27 0.2494623548846846 1107 28-29 0.2907582107857536 1107 30-31 0.3320918238465822 1107 32-33 0.36152971019419056 1107 34-35 0.35657661936333085 1107 36-37 0.17653898535026258 1107 38-39 0.06684931381375492 1107 40-41 0.07537950457015086 1107 42-43 0.03959858422655316 1107 44-45 -0.021636162522021607 1107 46-47 0.07222780352545755 1107 48-49 0.05324429927325269 1107 50-51 0.07362204246574322 1107 52-53 0.19642162769891058 1107 54-55 -0.043076950053865914 1107 56-57 1.2389175451221064 1107 58-59 -9.197748040734986 1107 60-61 -10.51421275458829 1107 62-63 -4.261618279066102 1107 64-65 -5.7828396859513305 1107 66-67 -6.85872331691025 1107 68-69 -3.830228228336866 1107 70-71 -3.9760587867206603 1107 72-73 -2.48091211216839 1107 74-75 -4.869498509663428 1107 76-77 -6.6603923019444835 1107 78-79 -7.445968298705667 1107 80-81 -3.0881988442455963 1107 82-83 -1.978917942668506 1107 84-85 -1.762914876386283 1107 86-87 -1.630477335232463 1107 88-89 -1.4277785856907776 1107 90-91 -1.2337784114229855 1107 92-93 -3.106551931366063 1107 94-95 -4.961623905995465 1107 96-97 -5.635387629985655 1107 98-99 -5.314894437307366 1107 100-101 -6.0823947700657435 1108 1 -0.13034906512354283 1108 2 -0.05318213544899919 1108 3 -0.13115557410713308 1108 4 -0.07120537043918063 1108 5 -0.03454832022762844 1108 6 -0.3248957772607426 1108 7 -0.10226157350319198 1108 8 -0.21885994067790904 1108 9 -0.4170445394003792 1108 10-11 -0.1426914032546378 1108 12-13 -0.21080287039937673 1108 14-15 -0.19174720074094154 1108 16-17 -0.10979126976027231 1108 18-19 -0.10229916327686084 1108 20-21 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11.094138250873078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 23.0 5 59.0 6 140.0 7 304.0 8 668.0 9 1183.0 10 1835.0 11 2948.0 12 4572.0 13 6390.0 14 8110.0 15 10912.0 16 14908.0 17 19884.0 18 22084.0 19 22053.0 20 23389.0 21 23556.0 22 22995.0 23 19617.0 24 18975.0 25 21904.0 26 31250.0 27 46471.0 28 65575.0 29 87670.0 30 104832.0 31 111557.0 32 107781.0 33 97431.0 34 85662.0 35 75036.0 36 57508.0 37 33926.0 38 11235.0 39 704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.38303558990006 17.615822396199707 13.546279941316996 20.45486207258324 2 13.786998935579337 22.94393993847339 39.6551724137931 23.61388871215417 3 23.32762757779931 28.74841057023453 30.22500049434597 17.698961357620195 4 11.991275445899683 15.106168228798595 39.733087794049624 33.16946853125209 5 12.200646552651856 41.44359452281229 33.19664756854018 13.159111355995678 6 26.93770558326756 37.26101616810843 18.661415427363874 17.139862821260127 7 25.725484329676036 36.82814141549614 19.721894091410082 17.72448016341774 8 22.39837405363766 31.957342980798497 21.328681549394826 24.31560141616902 9 26.003005620923798 20.786757385522467 21.281790717308287 31.92844627624545 10-11 23.11723125226754 24.58634024385421 28.064340577429984 24.23208792644826 12-13 31.51521638579242 22.28079858728939 25.907833356546078 20.296151670372108 14-15 26.931763569007185 23.612922528278883 28.90417118386584 20.55114271884809 16-17 20.162764345907764 27.747099696170928 30.82717335795604 21.262962599965267 18-19 19.623221424579803 31.0993423032283 30.220263938443022 19.057172333748873 20-21 21.697685775056442 28.45951610878697 26.393366656521838 23.449431459634752 22-23 20.698474872297044 27.420574415046744 25.07865504705547 26.802295665600738 24-25 22.748449474446204 27.21634452531651 27.83900498128365 22.196201018953637 26-27 25.457498260107393 23.82455692382688 29.466623507872825 21.251321308192907 28-29 21.6087610695555 24.809345714040067 31.854311753073684 21.727581463330754 30-31 20.629279682677396 30.338212893509393 29.145501579777132 19.88700584403608 32-33 20.603554263999104 28.92718659456113 27.218792719519218 23.25046642192054 34-35 22.329317441723003 25.207260846668888 27.529743033090803 24.93367867851731 36-37 24.734499088782886 25.393845895650042 29.370152099026814 20.501502916540257 38-39 26.931567518906224 22.216350709472863 30.824394815817623 20.027686955803286 40-41 21.690568558303198 25.1048621792913 32.825757060605305 20.378812201800194 42-43 20.044390540347447 28.79497372031808 29.76417148848187 21.396464250852603 44-45 20.909097169082933 29.800547264152648 26.418320523955074 22.872035042809348 46-47 21.01597774614284 27.25288458393653 24.26213522041268 27.469002449507947 48-49 23.040370643397313 26.065490030524032 26.302822644328565 24.59131668175009 50-51 26.058883675134524 27.45304178386138 26.420451611348465 20.06762292965563 52-53 21.7649134129289 27.273443046143917 30.090437775626544 20.871205765300633 54-55 18.842293644682105 26.476117547354068 31.240519507337027 23.4410693006268 56-57 21.30575279101687 30.084362740330057 26.46443817613937 22.1454462925137 58-59 19.75261345502883 27.025861748873304 28.78952617299148 24.431998623106388 60-61 21.07591400052119 24.736497054134517 29.26245105169969 24.925137893644596 62-63 27.315090658818654 23.877974064072042 26.810833490242004 21.996101786867303 64-65 24.528137846200462 25.095883147162283 29.879431058177214 20.496547948460037 66-67 20.195426321156692 25.757762339261042 31.635671066395606 22.41114027318666 68-69 21.01285162496465 28.95764943217301 28.771980917082963 21.257518025779376 70-71 20.56346435733094 28.041316887349133 27.3692984220964 24.025920333223528 72-73 19.484830062528722 26.69618492220222 26.979403003656895 26.839582011612162 74-75 21.28100614759762 26.96252172146772 27.877576250522612 23.878895880412053 76-77 21.43421276996591 25.1078380878326 29.92571808108168 23.532231061119806 78-79 20.09619424258101 24.942667120353043 31.117127470825768 23.84401116624018 80-81 20.28952283955688 30.00257626984513 27.89437895768165 21.81352193291634 82-83 20.718238337826158 28.732628552686386 25.808417906007946 24.740715203479514 84-85 21.611224667444727 25.61959846193857 25.908124687003635 26.861052183613072 86-87 23.348698212312165 25.753503093537766 27.67320308077072 23.22459561337935 88-89 25.47253026901878 22.87712794447506 28.743982949792503 22.906358836713654 90-91 21.065499270161702 25.08346704005427 30.62058449984636 23.23044918993767 92-93 18.62339755417811 28.372457592186628 29.3964777160704 23.607667137564857 94-95 18.369113360743043 28.925362055623115 27.53614414490297 25.16938043873087 96-97 18.10786328341164 27.019440557468695 26.438699944661998 28.433996214457668 98-99 19.66094066461631 26.20365540713811 27.028974256541403 27.106429671704174 100-101 22.695036692187077 26.715531716640577 26.754981947938717 23.834449643233636 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 713.0 1 536.5 2 361.5 3 375.0 4 403.0 5 487.5 6 634.0 7 831.0 8 1069.0 9 1225.5 10 1278.0 11 1312.5 12 1435.0 13 1556.0 14 1637.0 15 1700.0 16 1769.0 17 1916.5 18 1986.5 19 2127.5 20 2318.0 21 2516.0 22 2863.5 23 3228.0 24 3795.0 25 4502.5 26 5394.5 27 6648.0 28 7975.5 29 9382.0 30 11143.0 31 12942.5 32 14528.0 33 16053.5 34 17903.0 35 19845.0 36 21852.5 37 23750.0 38 24791.5 39 25333.0 40 26276.0 41 27867.0 42 30063.5 43 34117.5 44 41158.0 45 51368.5 46 65316.0 47 82660.0 48 101012.5 49 93190.0 50 65899.5 51 49409.5 52 38875.5 53 31130.5 54 25357.0 55 21383.5 56 18789.5 57 16684.0 58 14822.5 59 12893.5 60 11078.5 61 9378.0 62 7679.5 63 6247.0 64 4877.5 65 3713.0 66 2803.5 67 2173.5 68 1649.5 69 1215.5 70 959.0 71 748.5 72 629.5 73 539.0 74 428.0 75 309.5 76 221.5 77 171.0 78 114.0 79 64.0 80 49.0 81 31.5 82 14.0 83 7.0 84 5.5 85 4.0 86 1.5 87 2.0 88 2.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025448049011566828 2 0.00687785108420725 3 8.597313855259062E-5 4 0.0 5 0.13833077993111834 6 0.0 7 0.0 8 0.00137557021684145 9 0.0 10-11 0.0 12-13 0.0 14-15 6.018119698681344E-4 16-17 0.0 18-19 3.438925542103625E-4 20-21 0.0 22-23 4.7285226203924843E-4 24-25 0.0 26-27 6.447985391444298E-4 28-29 0.004642549481839894 30-31 0.014916339538874474 32-33 0.0037828180963139875 34-35 0.005717213713747276 36-37 0.10716551720580422 38-39 0.10617682611244943 40-41 0.11958863572665356 42-43 0.12208185674467868 44-45 0.11821306550981212 46-47 0.11073340245573673 48-49 0.11026055019369749 50-51 0.1091858859617901 52-53 0.08846635957061576 54-55 0.07728985155877897 56-57 0.5623932858417716 58-59 1.5946727604427273 60-61 0.53247463362547 62-63 0.004900468897497666 64-65 0.12496195688619048 66-67 1.9096783400994193 68-69 2.876016417430538 70-71 2.7839391860407137 72-73 2.6337441129893375 74-75 2.0152533542420006 76-77 1.4308939315000422 78-79 0.9551615693192819 80-81 0.05334633247188248 82-83 2.1493284638147655E-4 84-85 1.2895970782888594E-4 86-87 3.009059849340672E-4 88-89 8.597313855259062E-5 90-91 0.2549533423777075 92-93 0.40660995878447737 94-95 2.0128031197932517 96-97 2.4338995524238407 98-99 1.4347197361656323 100-101 0.9516796572079019 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1163154.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.36750350643416 #Duplication Level Percentage of deduplicated Percentage of total 1 69.13832358502434 23.069732546522793 2 14.088900761267064 9.402228911067633 3 6.132218478248111 6.138504647254925 4 3.3127782238783485 4.421565560051981 5 1.9665066598923135 3.2808708934691455 6 1.2699174785424843 2.542438555088902 7 0.8681183282180738 2.0276858952571435 8 0.6184459684526856 1.6508798416708044 9 0.4404522320655191 1.3227112258076663 >10 1.972997087378841 11.237355253328017 >50 0.10598635816269719 2.427871568790831 >100 0.06395166176162728 4.135736058521834 >500 0.008251827318722697 1.890400939847986 >1k 0.008767566526142866 6.115537387289748 >5k 0.002320826433390759 5.303964257355919 >10k+ 0.0020629568296806743 15.032516458674658 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 43259 3.7191120006465175 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 35112 3.0186888408585624 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 22703 1.9518481645594652 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 16950 1.4572446984664111 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 16926 1.455181343141149 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 14990 1.2887373469033336 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 13543 1.164334215417735 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 11223 0.9648765339757247 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 8981 0.7721247573408164 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 8068 0.6936312818423012 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 7623 0.6553732351863983 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 7389 0.6352555207650922 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7122 0.6123006927715504 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6159 0.5295085603454056 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5859 0.5037166187796285 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5301 0.45574360746728293 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 5140 0.4419019321603158 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4694 0.4035579123658604 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4042 0.3475034260295713 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3972 0.34148530633088997 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3878 0.33340383130694645 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3844 0.3304807445961584 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3383 0.29084712772341414 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 3046 0.26187418003119106 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2832 0.24347592838093665 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2774 0.2384894863448864 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2602 0.22370210651384081 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2430 0.2089147266827952 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2208 0.1898286899241201 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2099 0.18045761782188774 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1949 0.16756164703899912 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1870 0.16076976909334448 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1838 0.15801862865966157 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1706 0.1466701743707196 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1703 0.14641225495506183 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1573 0.13523574694322507 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1521 0.13076514373849035 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1517 0.13042125118428 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1450 0.12466105090125641 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1290 0.11090534873284191 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1266 0.10884199340757973 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1251 0.10755239632929088 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1231 0.10583293355823907 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1220 0.10488722903416058 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1203 0.10342568567876652 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1176 0.10110441093784657 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1166 0.10024467955232066 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.597313855259062E-5 0.0 5 0.0 0.0 0.0 8.597313855259062E-5 0.0 6 0.0 0.0 0.0 8.597313855259062E-5 0.0 7 0.0 0.0 0.0 8.597313855259062E-5 0.0 8 0.0 0.0 0.0 8.597313855259062E-5 0.0 9 0.0 0.0 0.0 8.597313855259062E-5 0.0 10-11 0.0 0.0 0.0 1.2895970782888594E-4 0.0 12-13 0.0 0.0 0.0 1.7194627710518125E-4 0.0 14-15 0.0 0.0 0.0 2.1493284638147655E-4 0.0 16-17 0.0 0.0 0.0 3.4389255421036255E-4 0.0 18-19 0.0 0.0 0.0 5.158388313155438E-4 0.0 20-21 0.0 0.0 0.0 9.457045240784969E-4 0.0 22-23 0.0 0.0 0.0 0.0022782881716436514 0.0 24-25 0.0 0.0 0.0 0.004040737511971759 0.0 26-27 0.0 0.0 0.0 0.005717213713747277 0.0 28-29 0.0 0.0 0.0 0.013325836475651547 0.0 30-31 0.0 0.0 0.0 0.035077040529456975 0.0 32-33 0.0 0.0 0.0 0.07690297243529232 0.0 34-35 0.0 0.0 0.0 0.15660007187354386 0.0 36-37 0.0 0.0 0.0 0.2687950176846746 0.0 38-39 0.0 0.0 0.0 0.3969809672665872 0.0 40-41 0.0 0.0 0.0 0.5516036569534215 0.0 42-43 0.0 0.0 0.0 0.700294200080127 0.0 44-45 0.0 0.0 0.0 0.8524666553182123 0.0 46-47 0.0 0.0 0.0 1.0548044369017344 0.0 48-49 0.0 0.0 0.0 1.2693933907290007 0.0 50-51 0.0 4.298656927629531E-5 0.0 1.4455953381925353 0.0 52-53 0.0 8.597313855259062E-5 0.0 1.5964781963523316 0.0 54-55 0.0 8.597313855259062E-5 0.0 1.7425035721839068 0.0 56-57 0.0 1.7194627710518125E-4 0.0 1.8546985179950377 0.0 58-59 0.0 1.7194627710518125E-4 0.0 1.9579952439659754 0.0 60-61 0.0 1.7194627710518125E-4 0.0 2.078916463340194 0.0 62-63 0.0 1.7194627710518125E-4 0.0 2.167984634880678 0.0 64-65 0.0 1.7194627710518125E-4 0.0 2.2252857317259798 0.0 66-67 0.0 1.7194627710518125E-4 0.0 2.27424743413168 0.0 68-69 0.0 1.7194627710518125E-4 0.0 2.344272555482765 0.0 70-71 0.0 1.7194627710518125E-4 0.0 2.462786526977511 0.0 72-73 0.0 1.7194627710518125E-4 0.0 2.6577736052147864 0.0 74-75 0.0 1.7194627710518125E-4 0.0 2.8853874895327705 0.0 76-77 0.0 1.7194627710518125E-4 0.0 3.1661757600455314 0.0 78-79 0.0 1.7194627710518125E-4 0.0 3.599007526088549 0.0 80-81 0.0 1.7194627710518125E-4 0.0 4.1405093392620405 0.0 82-83 0.0 1.7194627710518125E-4 0.0 4.674875381935668 0.0 84-85 0.0 1.7194627710518125E-4 0.0 5.265596816930518 4.298656927629531E-5 86-87 0.0 1.7194627710518125E-4 0.0 5.73539703255115 8.597313855259062E-5 88-89 0.0 1.7194627710518125E-4 0.0 6.037205735440018 8.597313855259062E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGGGA 255 0.0 25.84597 2 CGTTGGG 235 0.0 25.836628 62-63 TGGTAGC 1900 0.0 24.542078 62-63 ATGGGAG 1655 0.0 24.461708 5 GGTAGCA 1545 0.0 24.393744 64-65 CGGCTTG 670 0.0 24.238518 74-75 GTAGCAA 1495 0.0 24.233734 64-65 ATGGGAT 1800 0.0 24.060333 5 CGTGTAC 805 0.0 23.84221 76-77 TACATGG 8490 0.0 23.399673 2 GTACATG 8645 0.0 23.312883 1 GTGGTAG 2045 0.0 22.92069 62-63 CGGGAGT 330 0.0 22.824032 4 ACATGGG 8515 0.0 22.279564 3 CATGGGA 6445 0.0 22.277334 4 CTGCGCG 240 0.0 22.262562 62-63 GCAACTC 710 0.0 22.257292 68-69 TAGCAAC 1535 0.0 22.020153 66-67 ATACTCT 3530 0.0 21.870337 5 AGCAACT 750 0.0 21.723381 66-67 >>END_MODULE