FastQCFastQC Report
Fri 27 May 2016
SRR939628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133701
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA170731.5059526277210658No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA126811.1185488942851773No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT60470.5333857869050129No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA59060.5209486451895164No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG55590.4903409276343586No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT54210.47816840595536214No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC45690.4030163155893838No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG43400.3828169861365563No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG31830.28076185872641907No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG28290.24953669441942805No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT25140.22175159058693605No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG25010.22060490376210307No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA23650.2086087954407732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.17826569792211527No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT20130.1775600444914488No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20130.1775600444914488No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17050.15039238741078997No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA16790.14809901376112397No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA14820.1307222980309623No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC13560.11960825649796551No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT13120.11572716262929997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.11246351551246758No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT11390.1004674071911377No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTG1952.2983913E-531.887881
TATATGG2101.3496901E-924.7432042
GCTTTTA8800.024.7312281
GTCTTAA3001.545737E-524.1816441
GTACATG82800.024.156611
GGGATTC3750.023.9122897
GAGTACT35500.023.728512-13
ATTCGCG500.001709115523.61694780-81
TACATGG95550.022.6421342
ACATGGG93350.022.0634773
ATGGGAT19250.021.8201685
ATGGGAG18700.021.7047925
AGTACTT38200.021.68074412-13
CATGGGG14950.021.1688354
GTACTTT40350.020.99657814-15
CATGGGA71150.020.5802274
GATTAGA4751.2137825E-619.6362231
GCTTATA33650.019.4028051
ACCACTC8200.019.02305694-95
TTTACTC9200.018.996664
Produced by FastQC (version 0.11.5)