##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939628_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1133701 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.871454642802643 2.0 2.0 31.0 2.0 33.0 2 22.31114641338413 16.0 16.0 31.0 16.0 33.0 3 26.865920555772643 28.0 25.0 31.0 16.0 34.0 4 32.06066590750118 35.0 32.0 35.0 25.0 37.0 5 32.62423954817011 35.0 33.0 35.0 26.0 37.0 6 33.119660298438475 35.0 33.0 37.0 27.0 37.0 7 33.321342223390474 35.0 33.0 37.0 28.0 37.0 8 33.302428947315036 35.0 33.0 37.0 26.0 37.0 9 34.53860409402479 37.0 33.0 39.0 27.0 39.0 10-11 34.37019020006157 37.0 33.0 39.0 27.0 39.0 12-13 34.36500541147974 37.0 33.0 39.0 27.0 39.0 14-15 34.76601678925925 38.0 33.0 40.0 25.5 41.0 16-17 34.46237544114365 38.0 33.0 40.0 25.0 41.0 18-19 34.97076125010034 38.0 33.5 40.0 25.5 41.0 20-21 35.012494035023344 38.0 33.5 40.0 25.5 41.0 22-23 34.9192247338584 38.0 33.5 40.0 25.5 41.0 24-25 34.68313382452693 38.0 33.0 40.0 24.5 41.0 26-27 34.58049035856897 38.0 33.0 40.0 24.5 41.0 28-29 34.306544229916 38.0 33.0 40.0 24.0 41.0 30-31 34.291230227370356 38.0 33.0 40.0 24.0 41.0 32-33 34.083181103306785 38.0 33.0 40.0 24.0 41.0 34-35 33.87684142467899 38.0 33.0 40.0 23.0 41.0 36-37 33.452674029572165 37.0 31.5 40.0 19.0 41.0 38-39 33.08541581951502 37.0 31.0 40.0 17.0 41.0 40-41 32.62017939474341 37.0 30.5 39.5 15.5 41.0 42-43 32.496215933478055 37.0 30.0 39.0 15.0 41.0 44-45 32.29743865446004 37.0 30.0 39.0 14.5 41.0 46-47 31.898527477703556 36.0 30.0 39.0 9.0 40.0 48-49 31.491619042410655 36.0 30.0 39.0 8.5 40.0 50-51 30.78353948704288 35.5 28.5 38.5 8.0 40.0 52-53 30.58148047853887 35.0 28.0 38.5 7.0 40.0 54-55 30.752559537303043 35.0 28.0 39.0 4.0 40.0 56-57 30.6521155048818 35.0 28.0 39.0 2.0 40.0 58-59 30.343407124100622 35.0 28.0 39.0 2.0 40.0 60-61 29.64847212801259 34.5 26.5 38.0 2.0 40.0 62-63 29.02124678376397 34.0 26.0 38.0 2.0 40.0 64-65 28.130311695940996 33.0 25.5 37.0 2.0 39.0 66-67 27.54790637037455 32.5 23.5 36.5 2.0 39.0 68-69 27.35947926305084 33.0 24.0 36.0 2.0 39.0 70-71 26.71248283277513 32.0 22.5 35.5 2.0 38.0 72-73 26.044591563383996 31.5 21.5 35.0 2.0 37.5 74-75 25.389066429331898 31.0 19.5 35.0 2.0 37.0 76-77 24.569403220073017 30.5 15.5 34.0 2.0 36.0 78-79 23.891795102941607 30.0 10.5 34.0 2.0 36.0 80-81 23.242767713885762 29.5 7.0 34.0 2.0 35.0 82-83 22.6256918711371 29.0 2.0 34.0 2.0 35.0 84-85 22.023439160766372 29.0 2.0 34.0 2.0 35.0 86-87 21.103181967732233 28.0 2.0 33.0 2.0 35.0 88-89 20.044377662187827 26.0 2.0 32.5 2.0 34.0 90-91 18.987435840666983 25.0 2.0 32.0 2.0 34.0 92-93 18.09561956812246 24.5 2.0 32.0 2.0 34.0 94-95 17.23480132768693 21.0 2.0 31.0 2.0 34.0 96-97 16.123236638231774 14.0 2.0 31.0 2.0 34.0 98-99 14.99593323107239 2.0 2.0 31.0 2.0 34.0 100-101 13.498421541482278 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.917034278342346 1101 2 7.990710104007622 1101 3 3.9218488836458185 1101 4 2.7329772760804687 1101 5 1.6752104499658742 1101 6 1.5559267050610828 1101 7 1.192132784973687 1101 8 1.326673456562716 1101 9 1.1536082730287376 1101 10-11 1.138173596963263 1101 12-13 0.9766012268770723 1101 14-15 0.9972071665188622 1101 16-17 1.2115578528131081 1101 18-19 1.1601828565073973 1101 20-21 1.060528850601166 1101 22-23 1.0566541488272705 1101 24-25 1.3012404379138403 1101 26-27 1.405396993543583 1101 28-29 1.3597369221431777 1101 30-31 1.3608999487451072 1101 32-33 1.32736759679252 1101 34-35 1.4691535064969656 1101 36-37 1.5303541410383303 1101 38-39 1.3536487748169606 1101 40-41 1.3205541780387833 1101 42-43 1.23349299771089 1101 44-45 1.2280607971609 1101 46-47 1.2140498034619966 1101 48-49 1.2504210671743543 1101 50-51 1.1282518706746423 1101 52-53 1.3260866751826796 1101 54-55 1.3043182653045875 1101 56-57 1.2384233555535715 1101 58-59 1.1255698902605111 1101 60-61 1.0930127784323176 1101 62-63 0.9815129733497479 1101 64-65 0.8380595208309849 1101 66-67 0.6620462658551673 1101 68-69 0.5683035407471344 1101 70-71 0.7804917080407385 1101 72-73 0.8742331492334188 1101 74-75 0.8903906889962414 1101 76-77 0.49665064595668795 1101 78-79 0.5292535878394062 1101 80-81 0.3387585211156363 1101 82-83 0.10757280449477236 1101 84-85 -0.13091593521818723 1101 86-87 -0.5113199952300498 1101 88-89 -0.3567965876151362 1101 90-91 -0.029045149567828332 1101 92-93 -0.2840860105372016 1101 94-95 -0.31533781182931264 1101 96-97 -0.665228383561919 1101 98-99 -0.9062150711961046 1101 100-101 -0.9543803011865108 1102 1 14.628073970661106 1102 2 7.670379154875846 1102 3 3.60843142503181 1102 4 2.102432619461645 1102 5 1.494683679590537 1102 6 0.867265321618504 1102 7 0.9820031775309914 1102 8 0.5971399562911159 1102 9 0.8064649931774781 1102 10-11 0.5385513216185842 1102 12-13 0.3614412632930879 1102 14-15 0.5211176356179266 1102 16-17 0.5834055995565208 1102 18-19 0.682940972035496 1102 20-21 0.566533833513013 1102 22-23 0.5182242770971897 1102 24-25 0.6398626735113169 1102 26-27 0.5602868149145834 1102 28-29 0.8389652206409224 1102 30-31 0.9056060938526969 1102 32-33 0.49779763003506616 1102 34-35 0.5992331838758247 1102 36-37 0.7766212157763306 1102 38-39 0.7344585705478579 1102 40-41 0.9375724813125075 1102 42-43 0.7153736336914562 1102 44-45 0.7341638862760362 1102 46-47 0.646084917280966 1102 48-49 0.5326407531461754 1102 50-51 0.4104671402900486 1102 52-53 0.7233985021220768 1102 54-55 0.8045624267236775 1102 56-57 0.5936457285628229 1102 58-59 0.5089897526342284 1102 60-61 0.47147338800306926 1102 62-63 0.2610948327241296 1102 64-65 0.012061440053301453 1102 66-67 0.41905486576292716 1102 68-69 0.20469734598707845 1102 70-71 0.2863648311681253 1102 72-73 0.24179513752755 1102 74-75 0.1192147331547595 1102 76-77 0.04222712873258061 1102 78-79 -0.2606177510799874 1102 80-81 -0.05999146074575634 1102 82-83 -0.36450352023865307 1102 84-85 -0.5923283364731908 1102 86-87 -0.6789935911599336 1102 88-89 -0.48172773977312033 1102 90-91 -0.21592674137409773 1102 92-93 0.29996324571683175 1102 94-95 0.09814198050099421 1102 96-97 -0.4278381852233526 1102 98-99 -0.7714430151235199 1102 100-101 -0.8060736899640446 1103 1 14.937898718865402 1103 2 7.719628855798643 1103 3 3.802789414240209 1103 4 2.2977914624672096 1103 5 1.5825770095483946 1103 6 1.2269559194254427 1103 7 0.9168812880580148 1103 8 1.1082331144967483 1103 9 1.022690365845051 1103 10-11 0.981645988861068 1103 12-13 0.7372851325826915 1103 14-15 0.6180152370435721 1103 16-17 1.049939977302195 1103 18-19 0.8538505638509619 1103 20-21 0.6590830261286627 1103 22-23 0.8476081780567242 1103 24-25 0.9916748828107487 1103 26-27 1.08582906298237 1103 28-29 1.159256740250946 1103 30-31 0.9120741037607161 1103 32-33 1.1568257284004417 1103 34-35 1.060128839438157 1103 36-37 1.3089473042436168 1103 38-39 1.1874835937542656 1103 40-41 1.1596611943125552 1103 42-43 1.0162366141385064 1103 44-45 0.9308853299874755 1103 46-47 1.1184404418946485 1103 48-49 1.1383937079296764 1103 50-51 1.015088627684129 1103 52-53 0.9623521910718544 1103 54-55 1.0241244957918987 1103 56-57 1.168499106273753 1103 58-59 0.7631805564445457 1103 60-61 0.8180619843506065 1103 62-63 0.8846593241936027 1103 64-65 0.42265410931114644 1103 66-67 0.7666663433780521 1103 68-69 0.7390744711726036 1103 70-71 0.6496576960050824 1103 72-73 0.6487870909856817 1103 74-75 0.6298915411447155 1103 76-77 0.5064852311187451 1103 78-79 0.12184366671969826 1103 80-81 0.26490628215209355 1103 82-83 0.16213575790563084 1103 84-85 -0.045386245762205846 1103 86-87 -0.09184236978323312 1103 88-89 -0.29931050101271595 1103 90-91 -0.09089943972061221 1103 92-93 0.37451258228665196 1103 94-95 0.1297268911253049 1103 96-97 -0.44452570042540884 1103 98-99 -0.7394560256023901 1103 100-101 -0.8111759967055665 1104 1 14.914412990757032 1104 2 8.03950306963512 1104 3 4.015411571956708 1104 4 2.577876126231118 1104 5 1.7839775900999228 1104 6 1.4102457113944737 1104 7 1.2234560340667215 1104 8 0.9677009422664611 1104 9 1.133430569038083 1104 10-11 1.0226038988755803 1104 12-13 0.8357369951855063 1104 14-15 0.8799443613783566 1104 16-17 0.9882128365184499 1104 18-19 0.9692642068117721 1104 20-21 1.020279204164467 1104 22-23 1.0819502196629074 1104 24-25 1.3755041039041274 1104 26-27 1.2597731848981937 1104 28-29 1.2934355017410368 1104 30-31 1.4622216606063816 1104 32-33 1.4718535464942946 1104 34-35 1.2914725743196271 1104 36-37 1.2399935064690624 1104 38-39 1.099024474257412 1104 40-41 1.1515564531417795 1104 42-43 1.1845312440369113 1104 44-45 0.9416376280039245 1104 46-47 0.9917919174631038 1104 48-49 0.9238847577603444 1104 50-51 0.8769427641137355 1104 52-53 1.0074477401768398 1104 54-55 1.0842297207556157 1104 56-57 0.8313450182234376 1104 58-59 0.8297456265945193 1104 60-61 0.8143815295852441 1104 62-63 0.7547476164045364 1104 64-65 0.9595288069405576 1104 66-67 0.7544916941279318 1104 68-69 0.6490841470313349 1104 70-71 0.9476850303060012 1104 72-73 0.8282404049672927 1104 74-75 0.3619331473372611 1104 76-77 0.02371914500360006 1104 78-79 0.14564386023686993 1104 80-81 0.22668664015886364 1104 82-83 0.188298070435863 1104 84-85 0.12320107886285214 1104 86-87 -0.041184411389426856 1104 88-89 -0.3859360452797702 1104 90-91 -0.07359658534228686 1104 92-93 0.12992985369448817 1104 94-95 0.2033432433265645 1104 96-97 -0.6139112833958968 1104 98-99 -1.0066451886406078 1104 100-101 -1.0998697557766857 1105 1 14.673884257903083 1105 2 7.828254051567566 1105 3 3.7604094570252933 1105 4 2.2243460035650955 1105 5 1.4401356661165963 1105 6 1.2368110627752245 1105 7 0.9378538828793381 1105 8 0.8124837085967584 1105 9 0.9483710488253791 1105 10-11 0.8108527356633033 1105 12-13 0.7131993796196028 1105 14-15 0.7311761578615261 1105 16-17 0.8091071503742739 1105 18-19 0.8519719232901011 1105 20-21 0.9153689376831053 1105 22-23 0.8163166466373539 1105 24-25 0.9148871481968328 1105 26-27 0.6948411648365607 1105 28-29 1.0213634732196724 1105 30-31 0.7944299511375377 1105 32-33 0.5568239374544746 1105 34-35 0.8154241726035139 1105 36-37 0.681839237328937 1105 38-39 0.5592012220971583 1105 40-41 0.682974061456477 1105 42-43 0.8178380903720353 1105 44-45 0.8332806275809332 1105 46-47 0.6930632933770582 1105 48-49 0.722759614247142 1105 50-51 0.5828113966595119 1105 52-53 0.9363905766020046 1105 54-55 0.833030252509797 1105 56-57 0.6902334706979083 1105 58-59 0.6888772961818077 1105 60-61 0.8750341369935448 1105 62-63 0.5241811169181609 1105 64-65 0.9198608511728388 1105 66-67 0.7758452502340418 1105 68-69 0.9263573961702569 1105 70-71 0.7797899913308406 1105 72-73 0.7629518678955698 1105 74-75 0.5395580793573238 1105 76-77 0.6682629240839084 1105 78-79 0.218870943444319 1105 80-81 0.3525175060722141 1105 82-83 0.2931258545920059 1105 84-85 0.09058793003210752 1105 86-87 0.027233922044619163 1105 88-89 0.08122722742552924 1105 90-91 0.3886621614097834 1105 92-93 0.43192876601547425 1105 94-95 -0.08613829634350623 1105 96-97 -0.15098965671150566 1105 98-99 -0.39616812053981754 1105 100-101 -0.4191830073326699 1106 1 14.857750371608223 1106 2 7.917419407440963 1106 3 3.595013903326997 1106 4 2.3936717670543857 1106 5 1.708574760884936 1106 6 1.2595305149886755 1106 7 1.0293591364891626 1106 8 0.8189236974118685 1106 9 1.0014136908886115 1106 10-11 0.9963255915475528 1106 12-13 0.6420253201202328 1106 14-15 0.7657900902351358 1106 16-17 1.0664363809298436 1106 18-19 0.9311651235745657 1106 20-21 0.7875283086511402 1106 22-23 0.6082005991177155 1106 24-25 0.9800363120274938 1106 26-27 0.8317943129414189 1106 28-29 0.7237323871760353 1106 30-31 0.8775871514691076 1106 32-33 1.0885632180382245 1106 34-35 1.0092568618552988 1106 36-37 1.1645454462419949 1106 38-39 1.4376945610766683 1106 40-41 1.4880855042012158 1106 42-43 1.223266293517817 1106 44-45 1.2231141625191313 1106 46-47 1.2400075692146686 1106 48-49 1.256397659223527 1106 50-51 1.2541924757280896 1106 52-53 0.9570212329823775 1106 54-55 1.021215938957301 1106 56-57 1.309115341822487 1106 58-59 1.209068679232491 1106 60-61 1.0590819120756798 1106 62-63 1.0120814152024238 1106 64-65 1.117033815743909 1106 66-67 1.2864581806800537 1106 68-69 1.3676807714014423 1106 70-71 1.4647389432438942 1106 72-73 1.163263172255256 1106 74-75 1.1772257828785158 1106 76-77 1.2766391255755174 1106 78-79 1.108758728793731 1106 80-81 1.3547678131295378 1106 82-83 1.3608319178039672 1106 84-85 1.2071981039372268 1106 86-87 1.0625217995267278 1106 88-89 0.932636901110854 1106 90-91 1.0974118537324529 1106 92-93 1.3595528274058601 1106 94-95 0.9762003861837094 1106 96-97 0.7864475290623609 1106 98-99 0.4211378846397018 1106 100-101 0.26535488146452657 1107 1 14.883811397605207 1107 2 7.944634445214721 1107 3 3.9489307730029566 1107 4 2.392139590741877 1107 5 1.3698936523992913 1107 6 1.1682257375506993 1107 7 0.6830006779853974 1107 8 0.8286675336349134 1107 9 0.8778783160360604 1107 10-11 0.6727311132377238 1107 12-13 0.6275126461188094 1107 14-15 0.7120529097079213 1107 16-17 0.8893803552439294 1107 18-19 0.874628610568756 1107 20-21 1.0331504442072728 1107 22-23 0.9215257754826709 1107 24-25 0.6129907285623091 1107 26-27 0.6831960834833737 1107 28-29 0.8290103442092303 1107 30-31 1.1155191282083337 1107 32-33 1.1157514183287986 1107 34-35 0.9285995508979212 1107 36-37 0.9621246442648115 1107 38-39 0.9354155098060986 1107 40-41 1.0463691983563805 1107 42-43 0.9363687187723784 1107 44-45 0.9173480072691689 1107 46-47 0.8161897298516436 1107 48-49 1.0242140883987965 1107 50-51 0.9263280928499142 1107 52-53 0.8417331996941364 1107 54-55 0.843842133072819 1107 56-57 0.7977051418710381 1107 58-59 0.8335537350239584 1107 60-61 0.7574453745501692 1107 62-63 0.5894099633311818 1107 64-65 0.6241780757552036 1107 66-67 0.7999464557496161 1107 68-69 0.8909644228606091 1107 70-71 0.4626291511002023 1107 72-73 0.7915991019117712 1107 74-75 0.5129062777617079 1107 76-77 0.5414512814915362 1107 78-79 0.6004726558616476 1107 80-81 0.5393575473042667 1107 82-83 0.42875758773464057 1107 84-85 0.5053036825960611 1107 86-87 0.411519785495603 1107 88-89 0.6379689600703671 1107 90-91 0.7074793865992177 1107 92-93 0.870646772015732 1107 94-95 1.094162408705234 1107 96-97 0.6213710849312939 1107 98-99 0.04850461518770999 1107 100-101 -0.020483832580870498 1108 1 14.238896163833953 1108 2 7.533096819635123 1108 3 3.4952913796490144 1108 4 -0.12484021992273142 1108 5 0.24927807086915266 1108 6 0.5467721537021717 1108 7 0.584525745605184 1108 8 0.46220815380492297 1108 9 0.7571685498073109 1108 10-11 0.5508971681063528 1108 12-13 0.45112161057011946 1108 14-15 0.6191330633014331 1108 16-17 0.8302981730569101 1108 18-19 0.6014036298886936 1108 20-21 0.6512828099336971 1108 22-23 -0.3685425687986239 1108 24-25 0.39909184428874056 1108 26-27 0.43675034835973037 1108 28-29 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72-73 -0.18016618843929777 2312 74-75 0.2013884746186747 2312 76-77 0.06128961800886046 2312 78-79 0.531200477532618 2312 80-81 0.6037769411622094 2312 82-83 0.27588287693371427 2312 84-85 0.36379233543237177 2312 86-87 0.4821839593702464 2312 88-89 0.6904351921784233 2312 90-91 0.7594194203911115 2312 92-93 1.1438858976505344 2312 94-95 1.1388556656868118 2312 96-97 0.7691716119696039 2312 98-99 0.6441527091138113 2312 100-101 0.7677162451788817 2313 1 -14.152510086166046 2313 2 -7.3794031803648785 2313 3 -4.020333620350986 2313 4 -1.2568714699227286 2313 5 -0.8975969291308488 2313 6 -1.3704153462978326 2313 7 -0.5607867543948188 2313 8 -0.2815418461950756 2313 9 -2.4826752001926877 2313 10-11 -0.47566533189365146 2313 12-13 -0.8176283894298777 2313 14-15 -1.0320388116985697 2313 16-17 -0.8634518269430842 2313 18-19 -0.3118776201113107 2313 20-21 -1.3537953150663 2313 22-23 -2.3513550687986253 2313 24-25 -1.5290331557112538 2313 26-27 -0.4530934016402739 2313 28-29 0.005893434433339451 2313 30-31 -1.4055728105474614 2313 32-33 -0.41381352081339884 2313 34-35 -1.1180526660649832 2313 36-37 -1.2910281281463156 2313 38-39 -1.3605067757425928 2313 40-41 -0.6825901814736071 2313 42-43 -0.4161778905784743 2313 44-45 -0.39841645853453755 2313 46-47 -1.2589352459984333 2313 48-49 -0.7364637999319612 2313 50-51 -1.5410139666554912 2313 52-53 -0.46959552905392954 2313 54-55 -1.5103315773213097 2313 56-57 -1.0097487317765648 2313 58-59 -0.6600260080208642 2313 60-61 -2.236465826183988 2313 62-63 -1.0787079124416188 2313 64-65 -0.7019135007517505 2313 66-67 -1.9743664789489905 2313 68-69 -0.7497620068148159 2313 70-71 -0.5270866043093783 2313 72-73 -1.1299987777250138 2313 74-75 -0.5481990642011993 2313 76-77 -0.4833241242271633 2313 78-79 -0.31399556284005214 2313 80-81 -0.22709941753344154 2313 82-83 -0.0667680353333644 2313 84-85 -0.08697920959868455 2313 86-87 -0.42295724792789 2313 88-89 0.10178030087407564 2313 90-91 0.6566437992730982 2313 92-93 1.1875800940791024 2313 94-95 1.3321713683265628 2313 96-97 0.8752954473733325 2313 98-99 1.0836252440516994 2313 100-101 0.7423838499925459 2314 1 -14.152510086166046 2314 2 -7.169678820754076 2314 3 -3.544036708119055 2314 4 -1.69788057933496 2314 5 -1.2964843149529308 2314 6 -6.27510346683 2314 7 -2.068881596531437 2314 8 -4.370434940913103 2314 9 -1.3943784368884735 2314 10-11 -0.40281981958229096 2314 12-13 -3.261447889032741 2314 14-15 -3.063636930245032 2314 16-17 -2.522703030775496 2314 18-19 -1.3482228097459412 2314 20-21 -1.8796584753125316 2314 22-23 -1.2521983720154637 2314 24-25 -3.385004847033734 2314 26-27 -5.006340336211359 2314 28-29 -2.228753048390331 2314 30-31 -2.4795914389231584 2314 32-33 -4.15498551991983 2314 34-35 -3.0521685938846836 2314 36-37 -1.4293279941113681 2314 38-39 -1.7287634526687228 2314 40-41 -2.8016609966046637 2314 42-43 -2.140397881642812 2314 44-45 -3.134436179046851 2314 46-47 -2.7063677941517312 2314 48-49 -1.7755629113298959 2314 50-51 -2.6459471973941717 2314 52-53 -1.571294546130975 2314 54-55 -0.9771315549821509 2314 56-57 -1.4706446561212836 2314 58-59 -1.6977847699350797 2314 60-61 -1.1031708624230667 2314 62-63 -3.481858664526605 2314 64-65 -1.6097322060734314 2314 66-67 -2.5430252607202384 2314 68-69 -2.3966245961714527 2314 70-71 -3.4878000921965935 2314 72-73 -3.380452697006188 2314 74-75 -2.846336847163869 2314 76-77 -1.4555950237505968 2314 78-79 -1.1158087578360814 2314 80-81 -0.7270541186851496 2314 82-83 -0.819401263589917 2314 84-85 -0.7957727709569049 2314 86-87 -0.4489460659382125 2314 88-89 0.10416934972236902 2314 90-91 0.28180513763688 2314 92-93 0.8283009662991176 2314 94-95 0.716981051110519 2314 96-97 0.40517407128119487 2314 98-99 0.33276673779673516 2314 100-101 0.3015388713388525 2315 1 -14.152510086166046 2315 2 -7.363107728108634 2315 3 -4.744801035741798 2315 4 -2.992798267867933 2315 5 -3.6791774337642096 2315 6 -2.9421927687394103 2315 7 -3.121508324701022 2315 8 -3.5789878322949953 2315 9 -2.4568719165504618 2315 10-11 -5.015522302078985 2315 12-13 -5.025812313684515 2315 14-15 -3.6613315947364384 2315 16-17 -4.81751703393342 2315 18-19 -1.6077636243417643 2315 20-21 -3.4245680034224684 2315 22-23 -4.689573365535129 2315 24-25 -5.337676953555739 2315 26-27 -4.963683084859447 2315 28-29 -2.6939406837173436 2315 30-31 -3.5556203402614024 2315 32-33 -4.560844959673187 2315 34-35 -4.147945078937667 2315 36-37 -5.19341720449604 2315 38-39 -3.8343478061616096 2315 40-41 -4.977321622851527 2315 42-43 -3.769902835179604 2315 44-45 -2.4552755313588754 2315 46-47 -1.6799814662844952 2315 48-49 -1.5045005394565365 2315 50-51 -0.9728595045281736 2315 52-53 -0.8490715060885812 2315 54-55 -2.7267601757298507 2315 56-57 -4.867522830285829 2315 58-59 -4.064192884531742 2315 60-61 -4.936403817219443 2315 62-63 -2.8655006325262242 2315 64-65 -3.900689689309363 2315 66-67 -2.364459523872437 2315 68-69 -1.940817607137138 2315 70-71 -2.44978692159383 2315 72-73 -2.7769055691230804 2315 74-75 -3.1256850885767022 2315 76-77 -2.4642908345414263 2315 78-79 -2.015132498778808 2315 80-81 -2.0182538544794575 2315 82-83 -1.4114034729844533 2315 84-85 -1.4313241179387326 2315 86-87 -2.226900955119671 2315 88-89 -2.754717734984105 2315 90-91 -2.28424308630305 2315 92-93 -1.15877900392654 2315 94-95 -1.2786632030876177 2315 96-97 -1.9185892726105518 2315 98-99 -1.6106652097154246 2315 100-101 -1.230369662799557 2316 1 -14.152510086166046 2316 2 -7.364658780075862 2316 3 -5.326086871796072 2316 4 -3.3180134754966346 2316 5 -2.3629245251258943 2316 6 -1.3992054732590198 2316 7 -4.836698320249486 2316 8 -3.350207334427939 2316 9 -1.6460809598954143 2316 10-11 -4.960048073429572 2316 12-13 -4.434613319239958 2316 14-15 -2.4222696384945976 2316 16-17 -3.7387540694079924 2316 18-19 -3.000912379607591 2316 20-21 -3.681437114199248 2316 22-23 -3.564133118963781 2316 24-25 -3.989086507796312 2316 26-27 -4.849857762189405 2316 28-29 -4.1522000188077826 2316 30-31 -3.1697414341023773 2316 32-33 -3.4646118667052193 2316 34-35 -4.4980449890418654 2316 36-37 -2.8344691118374854 2316 38-39 -3.8025981516715746 2316 40-41 -3.518963142869147 2316 42-43 -7.455075882940157 2316 44-45 -4.276135350462699 2316 46-47 -5.4675944764897615 2316 48-49 -5.3981593253241975 2316 50-51 -4.107847936515114 2316 52-53 -5.470763210721973 2316 54-55 -5.579061900917509 2316 56-57 -5.774720217119256 2316 58-59 -4.4090976688383705 2316 60-61 -3.968113547591912 2316 62-63 -2.9029424680774554 2316 64-65 -3.1149966964577764 2316 66-67 -3.898709093523724 2316 68-69 -3.9715621719675838 2316 70-71 -3.9545232527404295 2316 72-73 -5.226718624442601 2316 74-75 -5.411673852599218 2316 76-77 -4.912196182443516 2316 78-79 -5.128741898513049 2316 80-81 -5.170084940861269 2316 82-83 -4.851715586427503 2316 84-85 -4.4316807733889405 2316 86-87 -4.330183649145891 2316 88-89 -4.006904412172993 2316 90-91 -3.489658327068767 2316 92-93 -3.5309695936789467 2316 94-95 -2.797127442057416 2316 96-97 -2.6595995257893428 2316 98-99 -2.6856456968029665 2316 100-101 -2.5493523829760765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8669.0 3 2587.0 4 2602.0 5 3054.0 6 3494.0 7 4439.0 8 5416.0 9 6918.0 10 8367.0 11 10048.0 12 11913.0 13 13189.0 14 14322.0 15 15806.0 16 17905.0 17 18817.0 18 17622.0 19 18305.0 20 19672.0 21 21845.0 22 23873.0 23 26294.0 24 29263.0 25 33028.0 26 36848.0 27 40759.0 28 45479.0 29 51127.0 30 57709.0 31 64876.0 32 73212.0 33 82724.0 34 93375.0 35 102756.0 36 94801.0 37 47259.0 38 5299.0 39 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.43843097639188 16.724046840800497 12.766846838478196 20.070675344329423 2 13.589754228976068 22.413639369270985 40.55199824140278 23.444608160350167 3 23.483922585385326 29.14996167418465 29.503106687060004 17.86300905337002 4 11.682771590525462 14.47154227255293 40.143327046904304 33.7023590900173 5 12.398737827123293 41.61284710416459 32.65364517328383 13.33476989542829 6 26.67431624192091 37.07190865735821 19.082421965057517 17.171353135663363 7 26.35842991129757 36.929046004945285 19.701286322192747 17.0112377615644 8 22.568525793294988 31.329862837991236 21.140104538881417 24.961506829832363 9 26.91239949441373 20.99322440204531 21.18365836359284 30.91071773994812 10-11 23.284391977733296 24.579882863990015 27.950888190883212 24.184836967393476 12-13 32.1416111016405 22.237728512918263 25.599011444525804 20.021648940915437 14-15 27.457544773976988 23.60426650177771 28.720491133537973 20.217697590707328 16-17 20.40740977915961 27.51100227222039 30.676741870021928 21.40484607859807 18-19 19.62714363260455 31.395629765437665 30.06524920177608 18.911977400181705 20-21 22.1601645250576 28.43818271382404 26.19805300233931 23.20359975877905 22-23 20.976057662396425 27.476333389892822 24.914718899352394 26.63289004835836 24-25 22.96199483576002 27.228415518885896 27.787977981703282 22.021611663650805 26-27 25.876028769754456 23.634236608867287 29.476181510739156 21.013553110639098 28-29 21.69721804700686 24.91131135448897 31.832716000549787 21.558754597954383 30-31 20.69358300683178 30.48191098884001 28.989934440373982 19.834571563954228 32-33 20.816035548908257 28.962910809949555 27.063568777670376 23.157484863471808 34-35 22.723865987536314 25.25774517056477 27.362527807312233 24.655861034586685 36-37 24.925779180524383 25.327647525891678 29.53465015361136 20.21192313997258 38-39 27.029940775904578 22.26249829891997 30.760696479603826 19.946864445571624 40-41 21.792199230845362 25.201258006136268 32.89695766821723 20.109585094801137 42-43 20.115269573515832 28.93381167187272 29.488731504513144 21.462187250098307 44-45 20.953087122400515 29.876502731274574 26.309761268661795 22.860648877663113 46-47 21.196275049470202 27.18428021019477 24.265748143734754 27.35369659660027 48-49 23.45095563980196 26.261850742415945 26.043543812331183 24.24364980545091 50-51 26.38527022881327 27.55387026930493 26.28527880455876 19.775580697323043 52-53 21.79026246706394 27.559815509429477 30.018053758015974 20.631868265490617 54-55 18.95916763120269 26.698696041614905 31.086060192353095 23.256076134829314 56-57 22.43480005852226 30.43153094497095 25.234276894271407 21.89939210223538 58-59 21.113140556627716 27.477042818060355 26.6892262068868 24.720590418425125 60-61 21.533244075630435 24.689210864695042 28.85873863842235 24.91880642125217 62-63 27.59529905427846 23.783999710123666 26.784263957147793 21.83643727845008 64-65 25.120709553021186 25.218443642070547 29.39021351275403 20.270633292154233 66-67 20.760500596095024 26.003176227606666 30.6484311963172 22.587891979981105 68-69 20.103820201684595 30.22277735166144 28.554885355068798 21.118517091585165 70-71 21.075158393554847 28.90252643133068 25.40743974681777 24.614875428296703 72-73 20.73154346398325 27.26234494512032 24.436065634632232 27.570045956264206 74-75 22.130731529613364 27.66254125923221 26.621389457973276 23.58533775318115 76-77 24.66758890711854 24.320975046012546 28.093053442640493 22.918382604228423 78-79 21.639017118464086 24.916865679163855 30.227161602863305 23.216955599508754 80-81 20.691095837721267 30.022291132267497 27.748436481767868 21.538176548243367 82-83 20.834543492570397 28.429834414850987 26.0435109161889 24.692111176389716 84-85 21.753942944418714 25.599661654534078 26.2584984108016 26.387896990245608 86-87 23.538228101432434 25.6709912128604 28.02903499695692 22.761745688750242 88-89 25.575407892747876 22.828645708562544 28.902064193217893 22.69388220547169 90-91 21.54015992731059 24.924583852238268 30.67874974920209 22.85650647124906 92-93 20.1413133979031 27.99986034828078 28.673370538436927 23.185455715379188 94-95 20.30629184820692 28.872781450951525 26.274782870115544 24.546143830726006 96-97 20.073719961437426 26.526405632258122 24.894970060939475 28.504904345364974 98-99 22.00136758850624 25.694210394557242 25.80509475371465 26.49932726322187 100-101 24.388102609464838 26.08562582927908 26.23361344537815 23.292658115877927 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2147.0 1 1505.5 2 810.0 3 657.5 4 569.0 5 599.5 6 838.0 7 1771.5 8 2301.0 9 1855.5 10 1473.5 11 1314.0 12 1300.0 13 1364.0 14 1427.0 15 1456.0 16 1526.5 17 1628.0 18 1705.0 19 1819.5 20 2065.5 21 2367.5 22 2715.5 23 3141.5 24 3678.5 25 4454.5 26 5457.0 27 6750.0 28 8263.0 29 9878.5 30 11429.5 31 12705.0 32 13835.5 33 15168.5 34 16677.5 35 18057.0 36 20033.0 37 22084.5 38 22863.5 39 22705.5 40 23010.5 41 23829.0 42 24413.5 43 26651.5 44 30095.0 45 35995.0 46 51695.5 47 98861.5 48 139766.0 49 108608.0 50 57955.0 51 37017.0 52 27899.5 53 24203.0 54 21988.0 55 19947.5 56 18259.5 57 16600.5 58 15330.0 59 14085.0 60 12394.5 61 10774.0 62 9053.0 63 7188.0 64 5661.5 65 4411.5 66 3490.0 67 2791.5 68 2069.5 69 1609.0 70 1307.5 71 1087.0 72 901.0 73 730.0 74 623.5 75 511.0 76 399.0 77 315.5 78 235.0 79 154.0 80 120.5 81 100.5 82 73.0 83 48.0 84 29.5 85 18.5 86 13.5 87 12.0 88 9.0 89 4.0 90 3.5 91 4.5 92 5.0 93 6.5 94 4.0 95 0.5 96 2.0 97 2.5 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 54.42105105314364 2 0.08644254525664175 3 0.11510971587746681 4 0.19079104631644497 5 0.12066673664396521 6 0.14218916627929234 7 0.15118624752028972 8 0.1483636337976239 9 0.13672035219162726 10-11 0.125915034034547 12-13 0.13619111211862742 14-15 0.14342405978295864 16-17 0.13658804217337728 18-19 0.14611436348737455 20-21 0.17275278049503354 22-23 0.18955615281277868 24-25 0.21698842992993744 26-27 0.20940265555027293 28-29 0.20777083199185675 30-31 0.222016210623436 32-33 0.21297502604302193 34-35 0.19881785409027602 36-37 0.18624840235652965 38-39 0.18232320514844744 40-41 0.18249961850611404 42-43 0.17694259773961565 44-45 0.19211414649894462 46-47 0.2173853599846873 48-49 0.22011976702851987 50-51 0.22969019168193375 52-53 0.256813745423176 54-55 0.24345043357992982 56-57 0.22117824717451956 58-59 0.17897135135278172 60-61 0.18929153277627875 62-63 0.19264338657194444 64-65 0.18161755171778096 66-67 0.19140849306827815 68-69 0.17932417806811496 70-71 0.19264338657194444 72-73 0.21240168263060544 74-75 0.24186271336093024 76-77 0.24340633024051314 78-79 0.2381580328499313 80-81 0.2432740202222632 82-83 0.2338800089265159 84-85 0.2040220481414412 86-87 0.21570943308685447 88-89 0.21698842992993744 90-91 0.20393384146260787 92-93 0.20419846149910778 94-95 0.24287709016751333 96-97 0.2708827106970886 98-99 0.28614246613525085 100-101 0.28234957894541857 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1133701.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.87510918265365 #Duplication Level Percentage of deduplicated Percentage of total 1 85.2213817048852 55.28746442801029 2 9.096407739770292 11.802608905750668 3 2.825222306095323 5.498598167196079 4 1.1650505953872519 3.0233113831625436 5 0.5748181059218932 1.864569369092449 6 0.31221494188968446 1.2152987066129484 7 0.19056530629181168 0.8654061536474982 8 0.12200206599898901 0.6331917881754981 9 0.07972794113265816 0.4655122997300442 >10 0.36702441728468505 4.126439496389291 >50 0.0219824409782429 0.9634321763554514 >100 0.016575755460421587 2.2327620655510434 >500 0.0023422263011838745 1.024274626811071 >1k 0.0036034250787444224 4.838742066198551 >5k 7.206850157488845E-4 2.6805534653267475 >10k+ 3.6034250787444225E-4 3.4778349019897985 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 17073 1.5059526277210658 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12681 1.1185488942851773 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6047 0.5333857869050129 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5906 0.5209486451895164 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5559 0.4903409276343586 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5421 0.47816840595536214 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4569 0.4030163155893838 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4340 0.3828169861365563 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3183 0.28076185872641907 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2829 0.24953669441942805 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2514 0.22175159058693605 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2501 0.22060490376210307 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2365 0.2086087954407732 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2021 0.17826569792211527 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2013 0.1775600444914488 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2013 0.1775600444914488 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1705 0.15039238741078997 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1679 0.14809901376112397 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1482 0.1307222980309623 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1356 0.11960825649796551 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1312 0.11572716262929997 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1275 0.11246351551246758 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1139 0.1004674071911377 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 4.410333941665395E-5 0.0 0.0 4.410333941665395E-5 0.0 14-15 8.82066788333079E-5 0.0 0.0 8.82066788333079E-5 0.0 16-17 1.764133576666158E-4 0.0 0.0 2.2051669708326977E-4 0.0 18-19 1.764133576666158E-4 0.0 0.0 4.851367335831935E-4 0.0 20-21 1.764133576666158E-4 0.0 0.0 7.497567700831171E-4 0.0 22-23 1.764133576666158E-4 0.0 0.0 0.0018964435949161197 0.0 24-25 1.764133576666158E-4 0.0 0.0 0.003484163813915662 0.0 26-27 1.764133576666158E-4 0.0 0.0 0.005865744142414975 0.0 28-29 1.764133576666158E-4 0.0 0.0 0.0128340717702463 8.82066788333079E-5 30-31 1.764133576666158E-4 0.0 0.0 0.03360674463549031 8.82066788333079E-5 32-33 1.764133576666158E-4 0.0 0.0 0.06955096626006328 8.82066788333079E-5 34-35 1.764133576666158E-4 4.410333941665395E-5 0.0 0.14801080708229067 8.82066788333079E-5 36-37 1.764133576666158E-4 8.82066788333079E-5 0.0 0.26082714931009143 8.82066788333079E-5 38-39 1.764133576666158E-4 8.82066788333079E-5 0.0 0.3807882325233902 8.82066788333079E-5 40-41 1.764133576666158E-4 8.82066788333079E-5 0.0 0.5224481587296828 8.82066788333079E-5 42-43 1.764133576666158E-4 8.82066788333079E-5 0.0 0.6634465348447254 8.82066788333079E-5 44-45 1.764133576666158E-4 8.82066788333079E-5 0.0 0.8112368252299327 8.82066788333079E-5 46-47 1.764133576666158E-4 8.82066788333079E-5 0.0 1.004806381929627 8.82066788333079E-5 48-49 1.764133576666158E-4 8.82066788333079E-5 0.0 1.2426997947430585 8.82066788333079E-5 50-51 1.764133576666158E-4 8.82066788333079E-5 0.0 1.4727428131403255 8.82066788333079E-5 52-53 1.764133576666158E-4 8.82066788333079E-5 0.0 1.66512157967577 8.82066788333079E-5 54-55 1.764133576666158E-4 8.82066788333079E-5 0.0 1.850664328601633 8.82066788333079E-5 56-57 1.764133576666158E-4 8.82066788333079E-5 0.0 2.0462626389144933 8.82066788333079E-5 58-59 1.764133576666158E-4 8.82066788333079E-5 0.0 2.3587347986814864 8.82066788333079E-5 60-61 1.764133576666158E-4 8.82066788333079E-5 0.0 2.8440479456223464 8.82066788333079E-5 62-63 1.764133576666158E-4 8.82066788333079E-5 0.0 3.243447787379565 8.82066788333079E-5 64-65 1.764133576666158E-4 8.82066788333079E-5 0.0 3.523062959281151 8.82066788333079E-5 66-67 1.764133576666158E-4 8.82066788333079E-5 0.0 3.8366377025335607 8.82066788333079E-5 68-69 1.764133576666158E-4 8.82066788333079E-5 0.0 4.1843043271550435 8.82066788333079E-5 70-71 1.764133576666158E-4 8.82066788333079E-5 0.0 4.6045209451169224 8.82066788333079E-5 72-73 1.764133576666158E-4 8.82066788333079E-5 0.0 5.109989318171193 8.82066788333079E-5 74-75 1.764133576666158E-4 8.82066788333079E-5 0.0 5.588201827465972 8.82066788333079E-5 76-77 1.764133576666158E-4 8.82066788333079E-5 0.0 5.995452063639355 8.82066788333079E-5 78-79 1.764133576666158E-4 8.82066788333079E-5 0.0 6.445791262422809 8.82066788333079E-5 80-81 1.764133576666158E-4 8.82066788333079E-5 0.0 6.959506959947993 8.82066788333079E-5 82-83 1.764133576666158E-4 8.82066788333079E-5 0.0 7.493598400283672 8.82066788333079E-5 84-85 1.764133576666158E-4 8.82066788333079E-5 0.0 8.27497726472853 8.82066788333079E-5 86-87 1.764133576666158E-4 8.82066788333079E-5 0.0 9.120835211400536 8.82066788333079E-5 88-89 1.764133576666158E-4 8.82066788333079E-5 0.0 9.74489746414619 8.82066788333079E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGTG 195 2.2983913E-5 31.88788 1 TATATGG 210 1.3496901E-9 24.743204 2 GCTTTTA 880 0.0 24.731228 1 GTCTTAA 300 1.545737E-5 24.181644 1 GTACATG 8280 0.0 24.15661 1 GGGATTC 375 0.0 23.912289 7 GAGTACT 3550 0.0 23.7285 12-13 ATTCGCG 50 0.0017091155 23.616947 80-81 TACATGG 9555 0.0 22.642134 2 ACATGGG 9335 0.0 22.063477 3 ATGGGAT 1925 0.0 21.820168 5 ATGGGAG 1870 0.0 21.704792 5 AGTACTT 3820 0.0 21.680744 12-13 CATGGGG 1495 0.0 21.168835 4 GTACTTT 4035 0.0 20.996578 14-15 CATGGGA 7115 0.0 20.580227 4 GATTAGA 475 1.2137825E-6 19.636223 1 GCTTATA 3365 0.0 19.402805 1 ACCACTC 820 0.0 19.023056 94-95 TTTACTC 920 0.0 18.99666 4 >>END_MODULE