FastQCFastQC Report
Fri 27 May 2016
SRR939627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045050
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62890.6017893880675567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42450.4062006602554902No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35190.33673029998564663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32120.3073537151332472No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25550.24448590976508303No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25240.241519544519401No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14110.13501746327926895No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13460.12879766518348404No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11940.11425290655949477No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG11560.11061671690349743No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11050.10573656762834313No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10790.10324864839002917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG71000.030.4722121
TACATGG71750.029.8891812
ATGGGCG1153.892832E-628.8855675
ACATGGG72050.027.7905243
CATGGGA53650.025.9153924
GAGTACT50450.024.45524812-13
CATGGGG19050.023.4149254
TGCACCG1252.270278E-422.7783345
ATGGGAT19500.022.1456015
ATGGGAG22900.021.9660055
GTACTTT57000.021.85316714-15
GTATCAA141600.021.6454581
AGTACTT51750.021.54851512-13
GTATAGG3052.1100277E-1020.2227861
CGGTATA1703.2160642E-719.5364932
ACTTTTT66800.019.39583416-17
TATCAAC159100.019.3540552
CAACGCA161500.019.3051665
TCAACGC161700.019.1042864
AACGCAG164200.019.0157136
Produced by FastQC (version 0.11.5)