##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939627_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1045050 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.478956030811922 31.0 31.0 33.0 27.0 34.0 2 30.60044399789484 31.0 31.0 34.0 26.0 34.0 3 30.646662839098607 31.0 31.0 34.0 26.0 34.0 4 34.26284771063585 35.0 35.0 37.0 30.0 37.0 5 34.27423759628726 35.0 35.0 37.0 30.0 37.0 6 34.43063298406775 37.0 35.0 37.0 30.0 37.0 7 34.25023300320559 36.0 35.0 37.0 30.0 37.0 8 34.26905124156739 36.0 35.0 37.0 30.0 37.0 9 35.54226113583082 38.0 35.0 39.0 30.0 39.0 10-11 35.45630687526912 38.0 35.0 39.0 29.5 39.0 12-13 35.32172336251854 37.0 35.0 39.0 29.0 39.0 14-15 35.99785464810296 38.0 34.5 40.0 27.0 41.0 16-17 35.78354863403665 38.0 34.0 40.0 27.0 41.0 18-19 35.84118319697622 38.0 34.0 40.0 27.0 41.0 20-21 35.9930261709966 39.0 34.5 40.0 27.0 41.0 22-23 35.86561982680254 38.5 34.0 40.0 27.0 41.0 24-25 35.74532462561599 38.0 34.0 40.0 27.0 41.0 26-27 35.85160566480073 38.0 34.5 40.0 27.0 41.0 28-29 35.73921295631788 38.0 34.0 40.0 27.0 41.0 30-31 35.49531744892589 38.0 34.0 40.0 27.0 41.0 32-33 35.250294722740534 38.0 34.0 40.0 26.0 41.0 34-35 35.01575905459069 38.0 34.0 40.0 25.0 41.0 36-37 34.70527725946127 38.0 33.5 40.0 24.5 41.0 38-39 34.53631883641931 38.0 33.0 40.0 24.0 41.0 40-41 34.26082101334865 38.0 33.0 40.0 24.0 41.0 42-43 33.907009234007944 38.0 33.0 40.0 22.5 41.0 44-45 33.605723649586146 38.0 33.0 40.0 20.5 41.0 46-47 33.074168700062195 37.5 31.5 40.0 16.5 41.0 48-49 32.6598373283575 37.0 31.0 40.0 14.0 41.0 50-51 32.00789675135161 36.5 30.5 39.5 9.5 40.5 52-53 32.03559686139419 37.0 31.0 39.0 9.0 40.0 54-55 32.09420649729678 37.0 31.0 39.5 8.0 40.5 56-57 32.00740108128797 37.0 31.0 39.5 8.0 41.0 58-59 31.658523037175254 36.0 30.5 39.0 7.0 40.0 60-61 31.252110903784505 36.0 29.5 39.0 2.0 40.0 62-63 30.860546385340413 35.0 29.0 39.0 2.0 40.0 64-65 30.279581359743553 35.0 28.0 38.0 2.0 40.0 66-67 29.763136692024304 34.0 28.0 38.0 2.0 40.0 68-69 29.27208698148414 34.0 27.0 37.5 2.0 39.5 70-71 28.729112961102338 34.0 26.0 37.0 2.0 39.0 72-73 28.000264102196066 33.0 26.0 36.0 2.0 39.0 74-75 27.54926367159466 33.0 25.0 36.0 2.0 38.0 76-77 27.18610640639204 33.0 25.0 35.0 2.0 38.0 78-79 26.620399502416152 32.0 23.5 35.0 2.0 37.0 80-81 25.971251614755275 32.0 21.5 35.0 2.0 36.5 82-83 25.3284804554806 31.0 20.0 34.5 2.0 36.0 84-85 24.700463135735134 31.0 18.0 34.0 2.0 35.5 86-87 24.04303430457873 31.0 11.5 34.0 2.0 35.0 88-89 23.249552652983112 30.0 4.5 34.0 2.0 35.0 90-91 22.04111143007512 29.0 2.0 34.0 2.0 35.0 92-93 20.40996363810344 26.5 2.0 33.0 2.0 35.0 94-95 20.21741352088417 27.0 2.0 33.0 2.0 34.0 96-97 19.598194344768192 26.5 2.0 33.0 2.0 34.0 98-99 18.622854887325964 25.0 2.0 32.5 2.0 34.0 100-101 16.96451222429549 21.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1212831619389796 1101 2 1.1593142221605035 1101 3 1.1433199072790607 1101 4 1.2376335497580015 1101 5 0.8712935682358918 1101 6 0.8871526785797172 1101 7 1.0625494181894055 1101 8 0.9869638584514462 1101 9 1.3394085981550887 1101 10-11 1.2935664996272607 1101 12-13 1.1689312103784104 1101 14-15 1.2680055807814838 1101 16-17 1.183879162035133 1101 18-19 0.8680649678577907 1101 20-21 1.1514499820931832 1101 22-23 1.338953189093452 1101 24-25 1.2782435316455363 1101 26-27 1.329516494919794 1101 28-29 1.4214031797059121 1101 30-31 1.4859733108333089 1101 32-33 1.5205551793387073 1101 34-35 1.6900333621706665 1101 36-37 1.6323965850131898 1101 38-39 1.508994711094111 1101 40-41 1.456582584202124 1101 42-43 1.1944747561130384 1101 44-45 1.004633456223452 1101 46-47 1.42919660181051 1101 48-49 1.4357356251636872 1101 50-51 1.6423592951462922 1101 52-53 1.5983353904590771 1101 54-55 1.505267790786057 1101 56-57 1.2510839768002668 1101 58-59 1.3176471917141122 1101 60-61 1.442281467680715 1101 62-63 1.404941391543634 1101 64-65 1.0237080647660797 1101 66-67 1.1676606155475469 1101 68-69 0.8694023702300804 1101 70-71 0.748866079955345 1101 72-73 0.9543907088589769 1101 74-75 0.9737194355321108 1101 76-77 0.820909022958638 1101 78-79 0.6726064454580509 1101 80-81 0.6641841105230064 1101 82-83 0.2236308796236166 1101 84-85 0.1946107355693023 1101 86-87 -0.3246895776311476 1101 88-89 -0.7666080806468258 1101 90-91 -3.929338631950113 1101 92-93 -9.499935305045753 1101 94-95 -7.702280948619091 1101 96-97 -4.314704287994237 1101 98-99 -3.1381528425273295 1101 100-101 -2.7189361279020723 1102 1 1.1587775784015548 1102 2 1.2314227199193866 1102 3 1.0448879186155686 1102 4 1.2771842482777913 1102 5 0.8783211239557431 1102 6 0.9819187861224989 1102 7 0.8920320360598097 1102 8 0.8144055971264876 1102 9 1.078722862745451 1102 10-11 0.9228484952220342 1102 12-13 0.9000915849760034 1102 14-15 1.1940102324457627 1102 16-17 1.437597027814597 1102 18-19 1.1890317284819147 1102 20-21 1.14546211954832 1102 22-23 1.396573369923054 1102 24-25 1.375545335320659 1102 26-27 1.2663820895472782 1102 28-29 1.3726704501647689 1102 30-31 1.265851012110204 1102 32-33 1.4683529551654502 1102 34-35 1.5229989521772893 1102 36-37 1.485564953696091 1102 38-39 1.5541250963196873 1102 40-41 1.2891592519469839 1102 42-43 1.3297311367485563 1102 44-45 1.3708846306916698 1102 46-47 1.193773485195436 1102 48-49 1.1903776626542566 1102 50-51 1.22045180473458 1102 52-53 1.3532643062516385 1102 54-55 1.632686040096786 1102 56-57 1.3866253103800474 1102 58-59 1.4451925146351066 1102 60-61 1.3978538380271317 1102 62-63 1.2180122601110135 1102 64-65 1.406883133693885 1102 66-67 1.428818063601394 1102 68-69 1.294469372679135 1102 70-71 1.3671099996756304 1102 72-73 1.0998360826094178 1102 74-75 1.0942680084569574 1102 76-77 0.7165161267585276 1102 78-79 0.639700156469555 1102 80-81 0.40022719232760195 1102 82-83 0.03145322301501707 1102 84-85 -0.10523292538290363 1102 86-87 -0.5109852663701169 1102 88-89 -0.827089343065385 1102 90-91 -4.848211758577037 1102 92-93 -10.494738764528526 1102 94-95 -8.64512862971824 1102 96-97 -4.752794410138932 1102 98-99 -3.218459513506799 1102 100-101 -2.5413411307516007 1103 1 0.9768306584888151 1103 2 0.9075679735963078 1103 3 0.7380152627517695 1103 4 0.9096633749779244 1103 5 1.0036754371663577 1103 6 0.7070329600114817 1103 7 0.8859729967077996 1103 8 0.877070848807918 1103 9 0.7322827751252134 1103 10-11 0.7990571284460728 1103 12-13 0.8870126088448131 1103 14-15 0.9080652989546039 1103 16-17 0.9039304699929502 1103 18-19 0.8643974930954954 1103 20-21 0.8905040022043238 1103 22-23 0.901478765461313 1103 24-25 0.9445026227213944 1103 26-27 1.0325801557341165 1103 28-29 1.0601406878550748 1103 30-31 1.0294623084090162 1103 32-33 1.0906125550693062 1103 34-35 0.9204266949583655 1103 36-37 0.9990424613265887 1103 38-39 1.0476499515331383 1103 40-41 0.9663481706170813 1103 42-43 1.056532408421191 1103 44-45 0.905690659798644 1103 46-47 0.7977312487931272 1103 48-49 0.7769984556809177 1103 50-51 0.8437119851451982 1103 52-53 0.7116002473302032 1103 54-55 0.7023984398119723 1103 56-57 0.7687087400865735 1103 58-59 0.8305214533508227 1103 60-61 0.6159072582550706 1103 62-63 0.6870095707320161 1103 64-65 0.6502056052014389 1103 66-67 0.596463768531617 1103 68-69 0.7231605793622187 1103 70-71 0.9173533136436198 1103 72-73 0.7739767738659857 1103 74-75 0.4054111305856054 1103 76-77 0.4078101126177458 1103 78-79 0.42070568035423506 1103 80-81 0.2516849219112238 1103 82-83 0.5097715221301975 1103 84-85 0.04921702178191012 1103 86-87 -0.4454248505821461 1103 88-89 -0.40565673854015216 1103 90-91 -1.9441983252876973 1103 92-93 -9.251214476216212 1103 94-95 -5.496338623505002 1103 96-97 -2.6576576282326023 1103 98-99 -1.7877439028635713 1103 100-101 -1.5052771480216762 1104 1 0.763560139425639 1104 2 0.7259473419881068 1104 3 0.6611523266031796 1104 4 0.7420671341240421 1104 5 0.8379684794646991 1104 6 0.7963658346265348 1104 7 1.0266756225351443 1104 8 0.8030916434787514 1104 9 0.9015782826157945 1104 10-11 0.7257777196234443 1104 12-13 0.774046534745068 1104 14-15 0.9116684975938512 1104 16-17 0.9725582410322531 1104 18-19 0.7880116909286343 1104 20-21 1.045870545736939 1104 22-23 0.8414272684743693 1104 24-25 0.6892882598166352 1104 26-27 0.8232413880958518 1104 28-29 0.8566606232662011 1104 30-31 0.8287253456765384 1104 32-33 0.9257029133634447 1104 34-35 0.6912743109207611 1104 36-37 0.5115952704319753 1104 38-39 0.5317404630123974 1104 40-41 0.32064610144604444 1104 42-43 0.4832952719951962 1104 44-45 0.2305515166475658 1104 46-47 0.23544015186210032 1104 48-49 0.2479188136485888 1104 50-51 0.23841981591832706 1104 52-53 0.36334475798744137 1104 54-55 0.298902135926177 1104 56-57 0.12947099929086647 1104 58-59 -0.049255013564117434 1104 60-61 -0.08916869394112581 1104 62-63 -0.007711736983406325 1104 64-65 -0.030268230696282927 1104 66-67 0.07862858400214279 1104 68-69 -0.04682861397163052 1104 70-71 0.1967085633311818 1104 72-73 0.0817173100808759 1104 74-75 -0.25064139768039695 1104 76-77 -0.14171681322475393 1104 78-79 -0.40612272636146685 1104 80-81 -0.5324081845072293 1104 82-83 -0.2489388935372041 1104 84-85 -0.1959398590342083 1104 86-87 -0.3417607964332987 1104 88-89 -0.5784650403365994 1104 90-91 -1.1328663095138154 1104 92-93 -4.805881055826855 1104 94-95 -2.32314694036884 1104 96-97 -1.6278526588403288 1104 98-99 -1.6235620997491402 1104 100-101 -1.1407819432181938 1105 1 0.6861826876865926 1105 2 0.7945341291652923 1105 3 0.6508888130199502 1105 4 0.7911173915501948 1105 5 0.5338496610455792 1105 6 0.4632620697045837 1105 7 0.6963112020277222 1105 8 0.5523590880985978 1105 9 0.7899280726509801 1105 10-11 0.6407896881338857 1105 12-13 0.5206055879946234 1105 14-15 0.6715592849361158 1105 16-17 0.8681549125482277 1105 18-19 0.7048305433961062 1105 20-21 0.6686677207557636 1105 22-23 0.6942004032955111 1105 24-25 0.9105109566522032 1105 26-27 0.8158049192191399 1105 28-29 0.731710921963078 1105 30-31 0.7283768659868883 1105 32-33 0.7915124386469259 1105 34-35 0.8904803653362094 1105 36-37 1.0402913218552996 1105 38-39 0.9792466794154464 1105 40-41 1.040444958246951 1105 42-43 1.0523889152716706 1105 44-45 0.763828618617211 1105 46-47 1.0949907136598611 1105 48-49 0.8522718434357373 1105 50-51 0.7859250005532346 1105 52-53 0.8582813558351745 1105 54-55 0.928894604018744 1105 56-57 0.7982889025316453 1105 58-59 0.7661229746943654 1105 60-61 0.7718057078912892 1105 62-63 0.7105355984753707 1105 64-65 1.1643038950556175 1105 66-67 1.024512345003373 1105 68-69 0.8902335294235009 1105 70-71 0.7276667664037895 1105 72-73 0.7451558321761915 1105 74-75 0.7873405670124995 1105 76-77 0.4275186981711272 1105 78-79 0.40975437973525075 1105 80-81 0.5062560368689439 1105 82-83 0.37045344661611423 1105 84-85 0.3764876850480867 1105 86-87 0.018914392638212263 1105 88-89 -0.12767909095514796 1105 90-91 -0.9729112946047671 1105 92-93 -7.07728250649286 1105 94-95 -2.729593943598278 1105 96-97 -1.006057588628508 1105 98-99 -0.18661731998004782 1105 100-101 -0.03417192062246954 1106 1 0.9175464752301536 1106 2 0.8581539287401476 1106 3 0.7532668732631791 1106 4 0.9502138877253401 1106 5 0.6803870491559962 1106 6 0.833596690039208 1106 7 0.7843081074403528 1106 8 0.8583653351160763 1106 9 0.8526332336707441 1106 10-11 0.8453234755328438 1106 12-13 0.8962762024292843 1106 14-15 0.6782780823852548 1106 16-17 0.9353663856585825 1106 18-19 0.9540171296613451 1106 20-21 0.8490196216132802 1106 22-23 0.9081155624353912 1106 24-25 0.8156253406991709 1106 26-27 0.7349009815736309 1106 28-29 0.8405890617400971 1106 30-31 0.8182626988502548 1106 32-33 0.861572667415011 1106 34-35 0.8774693607521726 1106 36-37 0.9556709872349671 1106 38-39 0.8957615088516278 1106 40-41 0.8495114484932174 1106 42-43 1.154074670047315 1106 44-45 1.211918361650774 1106 46-47 1.2090566254785742 1106 48-49 1.2040136333797804 1106 50-51 1.2484392804832467 1106 52-53 1.2727210764546726 1106 54-55 1.0932620211951516 1106 56-57 1.1873754208316498 1106 58-59 1.2796039516584834 1106 60-61 1.369950401541601 1106 62-63 1.486803439349952 1106 64-65 1.3291756287475742 1106 66-67 1.0611991120272215 1106 68-69 0.5137278102211518 1106 70-71 0.671168855973292 1106 72-73 0.7542570280751413 1106 74-75 0.7301883104220366 1106 76-77 0.4497755055402948 1106 78-79 0.7982534325601485 1106 80-81 0.9709954552600486 1106 82-83 1.0156586437876065 1106 84-85 0.7885558527342305 1106 86-87 0.4318032044034297 1106 88-89 0.6888997270281649 1106 90-91 0.24707916134074637 1106 92-93 -3.9562031606944146 1106 94-95 -1.2521562330144995 1106 96-97 -8.951227918849725E-4 1106 98-99 0.40306606324265815 1106 100-101 0.2185466888377121 1107 1 0.48721779490147554 1107 2 0.6380024585886765 1107 3 0.5082168782131831 1107 4 0.589086276597726 1107 5 0.42088303965199003 1107 6 0.3964122528547662 1107 7 0.4123152354474868 1107 8 0.27441325116399895 1107 9 0.4204483014858127 1107 10-11 0.22659760680696905 1107 12-13 0.3194645006175776 1107 14-15 0.46863299774016554 1107 16-17 0.7341363094285001 1107 18-19 0.5050888057330241 1107 20-21 0.5077167803104459 1107 22-23 0.4722800015998345 1107 24-25 0.6624350625088979 1107 26-27 0.6391678858405356 1107 28-29 0.9305677767188101 1107 30-31 0.7105505911381442 1107 32-33 0.4787269095692608 1107 34-35 0.6627876460704556 1107 36-37 0.5554012119651901 1107 38-39 0.26049024858036063 1107 40-41 0.3236229226046845 1107 42-43 0.5852324762051211 1107 44-45 0.5798398545722634 1107 46-47 0.8183156097375601 1107 48-49 0.5200343244004699 1107 50-51 0.6850792171231816 1107 52-53 0.7069355106691049 1107 54-55 0.3156038685370035 1107 56-57 0.43993704839328274 1107 58-59 0.4953127923673257 1107 60-61 0.27248480407600795 1107 62-63 0.26418934173585384 1107 64-65 0.3046607292326762 1107 66-67 0.06571599154409924 1107 68-69 0.08304025453359998 1107 70-71 -0.07782876802433236 1107 72-73 0.04351056732867775 1107 74-75 0.23803406826779394 1107 76-77 -0.10102392025913076 1107 78-79 -0.27704851774180383 1107 80-81 -0.2634120291474389 1107 82-83 -0.09882259569363683 1107 84-85 -0.16602785184947422 1107 86-87 -0.3957509981507705 1107 88-89 -0.8301577920293539 1107 90-91 -1.1798092655476786 1107 92-93 -4.20784903734029 1107 94-95 -1.9303440362023032 1107 96-97 -1.4140349109316723 1107 98-99 -1.3489881888115924 1107 100-101 -0.9513993561083343 1108 1 0.39021208743065117 1108 2 0.496061066647286 1108 3 0.12383575545996806 1108 4 -0.38542177349171425 1108 5 -1.0595454465904481 1108 6 -0.23515772755241926 1108 7 -0.02119698876241216 1108 8 0.16081754454503283 1108 9 0.2616187589353416 1108 10-11 0.1856656705261983 1108 12-13 0.22180924889256204 1108 14-15 0.24304947273804345 1108 16-17 0.4490502714354889 1108 18-19 0.36371177191413295 1108 20-21 0.14015991856753374 1108 22-23 0.003426181001820794 1108 24-25 -0.01714795137644387 1108 26-27 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2311 62-63 0.3090478473463385 2311 64-65 0.3654035264530471 2311 66-67 0.40526990728534784 2311 68-69 0.2799689854333067 2311 70-71 0.25236709152509107 2311 72-73 0.11905397730480516 2311 74-75 0.3954779875850676 2311 76-77 0.47304679794087434 2311 78-79 0.4946552622073561 2311 80-81 0.5374254359380473 2311 82-83 0.699022369367686 2311 84-85 0.46291905875970585 2311 86-87 0.4138972471834883 2311 88-89 0.7789881192273747 2311 90-91 1.8194755305708874 2311 92-93 2.857105247600799 2311 94-95 2.162387221106794 2311 96-97 1.7660116256452092 2311 98-99 1.713162061993014 2311 100-101 1.391665340848185 2312 1 0.09759705370455052 2312 2 -0.06328151721368869 2312 3 0.04226966139031063 2312 4 -0.009223032307026813 2312 5 0.2126804133232696 2312 6 0.1070157182743472 2312 7 0.21181229413893732 2312 8 -0.49778127934927596 2312 9 -0.1900384097014225 2312 10-11 -0.10362922998729118 2312 12-13 0.1552812670385535 2312 14-15 -0.1390094328006839 2312 16-17 0.0735775891062147 2312 18-19 0.05335176577356293 2312 20-21 0.14140839517543924 2312 22-23 0.09689509753716408 2312 24-25 0.10401223898099232 2312 26-27 0.19585142413528445 2312 28-29 0.20807638894721237 2312 30-31 0.3427410801587527 2312 32-33 0.1944760611765517 2312 34-35 0.19939041866272333 2312 36-37 0.2145470136241272 2312 38-39 0.2803168172247936 2312 40-41 0.4507647533367134 2312 42-43 0.49080187761515504 2312 44-45 0.2747464511863953 2312 46-47 0.45639753656730164 2312 48-49 0.44507506008043407 2312 50-51 0.36783006230835724 2312 52-53 0.44526489873580033 2312 54-55 0.48341161537276633 2312 56-57 0.440922563170389 2312 58-59 0.4289721715170707 2312 60-61 0.4934518113827693 2312 62-63 0.029371401357490612 2312 64-65 0.2714972348169944 2312 66-67 0.2319183676851786 2312 68-69 0.28104372508522957 2312 70-71 0.4687101425688702 2312 72-73 0.481088750966407 2312 74-75 0.4793881607602444 2312 76-77 0.4763923149942286 2312 78-79 0.9785237704486995 2312 80-81 0.9626068439817672 2312 82-83 1.2937145956614202 2312 84-85 1.0373313983574128 2312 86-87 0.6354029763044267 2312 88-89 1.0689460230114136 2312 90-91 1.5497459136572367 2312 92-93 2.334428664525852 2312 94-95 1.5876414080090626 2312 96-97 1.1476628046627155 2312 98-99 1.379864540478966 2312 100-101 1.2587237811163803 2313 1 -0.37804320224229215 2313 2 -0.835131719252427 2313 3 -0.3738399341278189 2313 4 -0.24166245956841692 2313 5 -0.8064720504917346 2313 6 -0.3148610800881926 2313 7 -1.0655733649988406 2313 8 -0.8629005895246209 2313 9 -0.9493914502367105 2313 10-11 -1.721786897971846 2313 12-13 -0.4817061315921265 2313 14-15 -0.19487571083958954 2313 16-17 -1.4697744186829382 2313 18-19 -1.2520168032819967 2313 20-21 -1.2565464875052186 2313 22-23 -0.8686160029284977 2313 24-25 -0.2048729551099555 2313 26-27 -0.7973346288147312 2313 28-29 -2.2482403157216098 2313 30-31 -0.47625771600764466 2313 32-33 -0.2384825387680749 2313 34-35 -0.07736843822416972 2313 36-37 0.13132151723717556 2313 38-39 -0.1956407281989243 2313 40-41 -0.30819478603362427 2313 42-43 0.04936122785607466 2313 44-45 -0.5583336208569776 2313 46-47 -0.5130796823593258 2313 48-49 -0.29617381618600547 2313 50-51 -0.16009218932708436 2313 52-53 -0.0394258840048991 2313 54-55 0.02797756647908045 2313 56-57 -0.30419677811177337 2313 58-59 -0.016034874175723246 2313 60-61 -0.30283123571605586 2313 62-63 -0.2855710665947164 2313 64-65 -0.18643469103681198 2313 66-67 -0.15767111572400339 2313 68-69 -0.22127451003871457 2313 70-71 -0.3325709914610506 2313 72-73 -0.09078275923818424 2313 74-75 0.2993714875093012 2313 76-77 0.09959628546764421 2313 78-79 0.2721230773889367 2313 80-81 0.5866032604770872 2313 82-83 0.176993284695957 2313 84-85 0.38489635715644255 2313 86-87 0.460512705581845 2313 88-89 0.7951601996296667 2313 90-91 1.667849144721746 2313 92-93 2.761432643087037 2313 94-95 1.8994133142033611 2313 96-97 1.3877164016988601 2313 98-99 1.2113651641705339 2313 100-101 1.1149356558305854 2314 1 -0.4150743073455416 2314 2 -0.44980145676257877 2314 3 -1.5135125945983425 2314 4 -0.11669509261916744 2314 5 -0.08092121260311558 2314 6 -2.6084992768538733 2314 7 -3.3934117997655733 2314 8 -1.1591374181846916 2314 9 -1.7172611486672764 2314 10-11 -1.132681451976147 2314 12-13 -1.9777032775015044 2314 14-15 -2.9040499625216896 2314 16-17 -2.012303492128517 2314 18-19 -2.4392308136316743 2314 20-21 -1.9330228227375414 2314 22-23 -2.987751062917255 2314 24-25 -1.5508601709621672 2314 26-27 -1.710923111229718 2314 28-29 -1.0526774496508509 2314 30-31 -1.2235549786752742 2314 32-33 -2.1793334325532996 2314 34-35 -0.6861373994280768 2314 36-37 -0.4639918161068337 2314 38-39 -0.26841123016331636 2314 40-41 -0.6420789439087216 2314 42-43 -0.6036273189441204 2314 44-45 -1.2240750838106536 2314 46-47 -1.4901384474766388 2314 48-49 -1.4711966793418085 2314 50-51 -0.8860041930044105 2314 52-53 -0.47854822711400047 2314 54-55 -0.4106662920208066 2314 56-57 -0.588419438822541 2314 58-59 -0.6928195216667028 2314 60-61 -0.505606206206167 2314 62-63 -0.5061128312940006 2314 64-65 -0.7386095337174758 2314 66-67 -0.5582848573214925 2314 68-69 -0.7682484852808393 2314 70-71 -0.6199116100395443 2314 72-73 -0.1684886597742441 2314 74-75 -0.014190488059554696 2314 76-77 -0.3936757003996405 2314 78-79 -0.4860804311244813 2314 80-81 -0.31227712557791065 2314 82-83 0.08966644006772029 2314 84-85 -0.31758751851283407 2314 86-87 -0.04333759400157078 2314 88-89 -0.3028322471204028 2314 90-91 0.45317692469730275 2314 92-93 1.6325076678721402 2314 94-95 0.8655562237421428 2314 96-97 0.7285584064813015 2314 98-99 0.6651948028285659 2314 100-101 0.9208699060391972 2315 1 -7.287215429531749 2315 2 -2.99950372891751 2315 3 -1.304648184651878 2315 4 -0.6555776180661681 2315 5 -1.1099604411914896 2315 6 -1.421873065430546 2315 7 -2.6815863584541084 2315 8 -2.6178251410743982 2315 9 -3.2936988126612974 2315 10-11 -1.965422985213614 2315 12-13 -2.6676877615346797 2315 14-15 -4.421721917614747 2315 16-17 -4.100862700570506 2315 18-19 -3.098621222977009 2315 20-21 -4.227235204641545 2315 22-23 -5.516015711695882 2315 24-25 -3.5996899746161404 2315 26-27 -4.602486405813757 2315 28-29 -2.9744688533178234 2315 30-31 -2.8346330540455043 2315 32-33 -1.6893282334858881 2315 34-35 -2.2787725954897837 2315 36-37 -2.9538528222888445 2315 38-39 -2.0808150677249486 2315 40-41 -3.2831783841966242 2315 42-43 -7.825526359553713 2315 44-45 -4.10281780308539 2315 46-47 -2.430325613903662 2315 48-49 -5.5538370744709304 2315 50-51 -6.626206794162826 2315 52-53 -6.3421437884101906 2315 54-55 -4.004550076616944 2315 56-57 -3.2262024427462137 2315 58-59 -3.946843924789391 2315 60-61 -2.9076557760645727 2315 62-63 -2.9636470111005018 2315 64-65 -1.4958565462845996 2315 66-67 -2.226059575231467 2315 68-69 -1.720506424013081 2315 70-71 -1.6497449149404773 2315 72-73 -2.0904019165838044 2315 74-75 -2.770519540285811 2315 76-77 -2.6191434133786267 2315 78-79 -1.5415792572725415 2315 80-81 -1.447086912822325 2315 82-83 -1.7030028748739134 2315 84-85 -1.4268881627097834 2315 86-87 -1.8050050324048073 2315 88-89 -1.0378698997414624 2315 90-91 -0.21830050403891832 2315 92-93 0.3917376372463828 2315 94-95 0.6134164825582182 2315 96-97 0.4121679402711784 2315 98-99 0.27584598602258126 2315 100-101 -0.031195910904887825 2316 1 -4.06786672302708 2316 2 -2.6046446496547624 2316 3 -4.235485443929505 2316 4 -4.195564393832758 2316 5 -3.9933640282648035 2316 6 -4.486432119897859 2316 7 -3.1185480908286856 2316 8 -1.38966578906183 2316 9 -1.9832516682692045 2316 10-11 -2.9386273822712283 2316 12-13 -3.754975967171511 2316 14-15 -2.74698433599643 2316 16-17 -2.642382660897802 2316 18-19 -3.167172342904273 2316 20-21 -5.55693187378775 2316 22-23 -6.463346069576698 2316 24-25 -4.177630300262891 2316 26-27 -3.7810424848285464 2316 28-29 -3.7879245108101784 2316 30-31 -5.972970933209069 2316 32-33 -4.434112926435716 2316 34-35 -6.0790949361790965 2316 36-37 -9.295029779062133 2316 38-39 -5.92795532725053 2316 40-41 -5.147320048796999 2316 42-43 -4.100553221277821 2316 44-45 -3.043631093439963 2316 46-47 -5.7850491447739785 2316 48-49 -3.0648793093663755 2316 50-51 -4.2751116623337495 2316 52-53 -5.290722183318863 2316 54-55 -7.095979120339564 2316 56-57 -5.189480399602154 2316 58-59 -5.723668455742363 2316 60-61 -6.2223718683036005 2316 62-63 -6.23052030641416 2316 64-65 -4.7382980703775 2316 66-67 -6.112992379595465 2316 68-69 -4.796578826690986 2316 70-71 -5.9390084904425855 2316 72-73 -8.087157846825974 2316 74-75 -8.00732836746161 2316 76-77 -7.102286648356724 2316 78-79 -5.926933418773494 2316 80-81 -6.053893260454355 2316 82-83 -5.073273145144185 2316 84-85 -6.499100742261813 2316 86-87 -4.974469450951798 2316 88-89 -5.139580784938584 2316 90-91 -6.150530440397294 2316 92-93 -4.33598779460749 2316 94-95 -4.393042728946867 2316 96-97 -5.537794866572362 2316 98-99 -6.259239558237276 2316 100-101 -5.279526351437164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3938.0 3 1414.0 4 1504.0 5 1618.0 6 1974.0 7 2477.0 8 3121.0 9 3834.0 10 4917.0 11 6195.0 12 7868.0 13 9624.0 14 10393.0 15 12510.0 16 14439.0 17 16442.0 18 14293.0 19 11862.0 20 12542.0 21 13414.0 22 15039.0 23 17067.0 24 19520.0 25 22618.0 26 25643.0 27 29157.0 28 33587.0 29 38749.0 30 44665.0 31 51733.0 32 60170.0 33 70285.0 34 83923.0 35 101375.0 36 120428.0 37 114866.0 38 41051.0 39 795.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.17990710147009 19.611550064645883 13.848680745103673 21.35986208878035 2 14.97197489809054 21.771270726698745 41.04432295338217 22.212431421828548 3 19.016322472755974 28.058619916826455 30.73915650303329 22.185901107384286 4 11.85694240245964 17.15101215932111 39.40376704292398 31.588278395295266 5 12.233320658831461 39.74778060533038 34.08955732785744 13.929341407980722 6 28.565108306360486 37.85867504261391 18.974584873498937 14.60163177752667 7 26.538751070273126 35.11989932249556 21.916224670202638 16.425124937028674 8 25.800411997536827 34.06009111447153 20.86314537422152 19.276351513770123 9 27.765179518806093 16.70666401081748 21.07090734278458 34.45724912759185 10-11 24.04682392759013 27.18918525484908 28.31553764867114 20.448453168889653 12-13 26.584295101089765 25.16110866898238 28.20021816350004 20.054378066427816 14-15 23.09210330986543 25.760472205807567 28.123895602138067 23.02352888218894 16-17 20.854586828228346 29.25205575486859 28.34987961407548 21.543477802827585 18-19 20.6993697512191 27.991646486031424 31.257006555334364 20.05197720741511 20-21 22.322023800401528 26.627513371442586 30.630565048811516 20.419897779344378 22-23 21.582745528540563 26.987963233043704 30.380970307775502 21.048320930640234 24-25 21.387987712920786 27.091074237950057 30.85413596653438 20.666802082594774 26-27 21.569278457694637 26.770534647014156 31.13931388683277 20.520873008458437 28-29 20.71669246575572 27.39599757196317 31.26729820537033 20.620011756910777 30-31 21.00961600652342 27.312026499952808 31.04454715273549 20.63381034078828 32-33 20.905371481133702 27.383918134258284 30.663003276929267 21.047707107678747 34-35 21.457437900130493 27.089062029927508 30.559047332527246 20.894452737414753 36-37 21.76841677621619 26.723919867237473 30.845345454684825 20.662317901861513 38-39 21.450788718940704 26.952779378576945 30.906841152977798 20.68959074950455 40-41 21.136736943303983 27.08201304172672 31.064611298965755 20.716638716003537 42-43 20.876104087837465 27.51962436942627 30.292160838634917 21.312110704101343 44-45 20.993296215685973 27.553434851248408 29.99933900954892 21.4539299235167 46-47 21.38177703448167 27.442046297808897 29.40285973117741 21.77331693653202 48-49 21.472355599339345 27.601756185724835 29.195461669564732 21.73042654537109 50-51 21.431063301232903 27.86464722750414 29.340698414402254 21.363591056860702 52-53 21.36618767923689 28.242606238594014 29.01092865796617 21.380277424202923 54-55 20.7445618080805 27.72743457660194 29.35643609432605 22.17156752099151 56-57 21.39094307949095 28.324195671795383 28.540069332525924 21.744791916187744 58-59 21.42600857764648 27.654790207897513 28.8391626615135 22.08003855294251 60-61 21.361056608979567 27.5997037723991 29.358914419731136 21.6803251988902 62-63 21.709688050261445 27.39001477263365 29.1957824226736 21.70451475443131 64-65 21.215977455042516 27.729071605815747 29.315628925862473 21.739322013279264 66-67 21.075876181598534 27.76626024243667 28.960576498415914 22.197287077548886 68-69 20.84258799331509 28.001707152461076 29.071407734971764 22.08429711925207 70-71 21.009147679260217 27.953451074095437 28.760914844571843 22.276486402072504 72-73 21.13842252695071 27.572667969360186 28.689940094625765 22.598969409063336 74-75 20.874065113978695 28.103706572070212 28.56588987538687 22.45633843856422 76-77 21.070046733607892 27.63489410771208 28.749340404800943 22.54571875387909 78-79 20.913524440495397 27.499379366599285 28.88331996215814 22.703776230747184 80-81 20.779575574314755 28.105201052700696 28.603081895838994 22.512141477145555 82-83 20.719661550008556 27.934825275239845 28.532490307713616 22.813022867037983 84-85 20.745418696080286 27.474694719491715 28.420543216028634 23.359343368399365 86-87 20.72851264796206 27.434704545465436 28.70479559277544 23.13198721379706 88-89 21.040299142677483 26.891784120362562 28.80762771651611 23.260289020443846 90-91 20.392163823088794 27.11044933919014 29.27514068383063 23.22224615389043 92-93 20.141142201345044 27.35250630339609 29.3841190011919 23.122232494066967 94-95 20.437653603867844 27.16531131022901 28.67997484826526 23.717060237637885 96-97 20.327784616210536 26.865645886778562 28.50083019851906 24.305739298491837 98-99 20.473596830478648 26.76052691758391 28.372278769451743 24.3935974824857 100-101 20.519097584304813 26.78576137428772 28.69094108311938 24.004199958288087 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1211.0 1 757.5 2 339.5 3 379.5 4 350.5 5 367.5 6 519.5 7 909.0 8 1421.5 9 1627.5 10 1540.5 11 1451.5 12 1491.5 13 1611.5 14 1716.0 15 1811.5 16 1927.5 17 2059.0 18 2273.0 19 2495.0 20 2755.0 21 3152.5 22 3627.0 23 4157.5 24 4906.5 25 6033.5 26 7467.5 27 9131.0 28 10789.0 29 12336.0 30 14400.0 31 17024.0 32 19426.0 33 22095.5 34 25265.0 35 27096.0 36 29040.0 37 31879.0 38 32518.5 39 31664.5 40 31613.0 41 32516.5 42 33897.5 43 35331.5 44 37009.5 45 38313.0 46 39036.0 47 41433.0 48 49594.0 49 49655.0 50 39727.5 51 33750.5 52 30411.0 53 28400.0 54 26319.5 55 24040.5 56 21767.5 57 19271.0 58 17001.0 59 15108.0 60 13313.0 61 11318.0 62 9258.5 63 7118.5 64 5207.5 65 3901.0 66 2813.5 67 2050.0 68 1509.0 69 1018.5 70 700.5 71 528.5 72 421.0 73 352.5 74 277.0 75 200.5 76 147.0 77 87.0 78 53.0 79 37.0 80 27.5 81 23.0 82 15.5 83 15.0 84 11.0 85 5.5 86 4.5 87 5.0 88 5.5 89 2.0 90 2.0 91 2.5 92 2.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08612028132625234 2 0.09434955265298312 3 0.09262714702645806 4 0.09521075546624563 5 0.09176594421319555 6 0.07559446916415483 7 0.08841682216161906 8 0.08315391608057031 9 0.07789100999952155 10-11 0.0844935649012009 12-13 0.08411080809530645 14-15 0.08889526816898713 16-17 0.08688579493804124 18-19 0.08980431558298646 20-21 0.09894263432371657 22-23 0.107506817855605 24-25 0.12937180039232574 26-27 0.13563944308884743 28-29 0.13477824027558488 30-31 0.13640495670063632 32-33 0.13300799004832303 34-35 0.127075259556959 36-37 0.13224247643653414 38-39 0.12444380651643462 40-41 0.11927658963685947 42-43 0.12042486005454285 44-45 0.11119085211233912 46-47 0.1292282665901153 48-49 0.1472178364671547 50-51 0.15865269604325152 52-53 0.16630783216114062 54-55 0.16377206832208985 56-57 0.13243385483948136 58-59 0.12348691450169848 60-61 0.12037701545380604 62-63 0.11750633940959762 64-65 0.10444476340844934 66-67 0.11779340701401847 68-69 0.11544902157791494 70-71 0.12324769149801446 72-73 0.15032773551504713 74-75 0.16659489976556147 76-77 0.171905650447347 78-79 0.16850868379503373 80-81 0.17496770489450264 82-83 0.1744892588871346 84-85 0.14760059327304914 86-87 0.15133247213052006 88-89 0.1724319410554519 90-91 0.2612793646237022 92-93 0.5292569733505573 94-95 0.17051815702597964 96-97 0.18429740203817999 98-99 0.20252619491890342 100-101 0.20884168221616192 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1045050.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.504697740340724 #Duplication Level Percentage of deduplicated Percentage of total 1 79.79764876091808 45.88739672386469 2 11.37300352239901 13.08002259910771 3 3.752631355698462 6.473817955210953 4 1.6440587833060614 3.781644136054696 5 0.8666642539469268 2.4918632982787967 6 0.5443315432797751 1.8780972520101997 7 0.36239569797332916 1.4587618552049273 8 0.2651373125813279 1.21973128157404 9 0.2008482020964558 1.0394743637922803 >10 1.0650769866006802 11.174660203388243 >50 0.0833906907050168 3.348025426162015 >100 0.04007794441942507 4.203834566834929 >500 0.002705683471749035 1.0808608788371 >1k 0.0018601573868274616 2.270245441055415 >5k 1.691052169843147E-4 0.6115640186240685 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6289 0.6017893880675567 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4245 0.4062006602554902 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3519 0.33673029998564663 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3212 0.3073537151332472 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2555 0.24448590976508303 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2524 0.241519544519401 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1411 0.13501746327926895 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1346 0.12879766518348404 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1194 0.11425290655949477 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1156 0.11061671690349743 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1105 0.10573656762834313 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1079 0.10324864839002917 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.8706760442084113E-4 0.0 0.0 0.0 6 0.0 2.8706760442084113E-4 0.0 0.0 0.0 7 0.0 2.8706760442084113E-4 0.0 0.0 0.0 8 0.0 2.8706760442084113E-4 0.0 0.0 0.0 9 0.0 2.8706760442084113E-4 0.0 0.0 0.0 10-11 0.0 3.827568058944548E-4 0.0 0.0 0.0 12-13 0.0 3.827568058944548E-4 0.0 0.0 0.0 14-15 0.0 3.827568058944548E-4 0.0 0.0 0.0 16-17 0.0 3.827568058944548E-4 0.0 0.0 0.0 18-19 4.784460073680685E-5 3.827568058944548E-4 0.0 4.784460073680685E-5 0.0 20-21 9.56892014736137E-5 4.784460073680685E-4 0.0 1.913784029472274E-4 0.0 22-23 9.56892014736137E-5 4.784460073680685E-4 0.0 6.21979809578489E-4 0.0 24-25 9.56892014736137E-5 4.784460073680685E-4 0.0 0.001913784029472274 0.0 26-27 9.56892014736137E-5 4.784460073680685E-4 0.0 0.0031098990478924454 0.0 28-29 9.56892014736137E-5 5.741352088416823E-4 0.0 0.009568920147361371 0.0 30-31 9.56892014736137E-5 5.741352088416823E-4 0.0 0.02339600976029855 0.0 32-33 9.56892014736137E-5 5.741352088416823E-4 0.0 0.05454284483995981 0.0 34-35 9.56892014736137E-5 5.741352088416823E-4 0.0 0.09884694512224296 0.0 36-37 9.56892014736137E-5 5.741352088416823E-4 0.0 0.17123582603703172 0.0 38-39 9.56892014736137E-5 5.741352088416823E-4 0.0 0.28319219176115973 0.0 40-41 9.56892014736137E-5 5.741352088416823E-4 0.0 0.4201712836706378 0.0 42-43 9.56892014736137E-5 6.21979809578489E-4 0.0 0.553609875125592 0.0 44-45 9.56892014736137E-5 6.698244103152959E-4 0.0 0.7049901918568489 0.0 46-47 1.4353380221042054E-4 6.698244103152959E-4 0.0 0.8885220802832401 0.0 48-49 1.913784029472274E-4 6.698244103152959E-4 0.0 1.0937275728434046 0.0 50-51 1.913784029472274E-4 6.698244103152959E-4 0.0 1.3158222094636622 0.0 52-53 1.913784029472274E-4 6.698244103152959E-4 0.0 1.5372948662743409 0.0 54-55 1.913784029472274E-4 6.698244103152959E-4 0.0 1.7705851394670111 0.0 56-57 1.913784029472274E-4 6.698244103152959E-4 0.0 2.0286589158413473 0.0 58-59 1.913784029472274E-4 6.698244103152959E-4 0.0 2.3222333859623943 0.0 60-61 1.913784029472274E-4 6.698244103152959E-4 0.0 2.683890722931917 0.0 62-63 1.913784029472274E-4 6.698244103152959E-4 0.0 3.0421989378498635 0.0 64-65 1.913784029472274E-4 7.176690110521028E-4 0.0 3.374814602172145 0.0 66-67 1.913784029472274E-4 7.655136117889096E-4 0.0 3.747093440505239 0.0 68-69 1.913784029472274E-4 7.655136117889096E-4 0.0 4.176546576718817 0.0 70-71 1.913784029472274E-4 7.655136117889096E-4 0.0 4.670781302330032 0.0 72-73 1.913784029472274E-4 7.655136117889096E-4 0.0 5.191761159753122 0.0 74-75 1.913784029472274E-4 7.655136117889096E-4 0.0 5.698435481555906 0.0 76-77 1.913784029472274E-4 7.655136117889096E-4 0.0 6.213339074685422 0.0 78-79 1.913784029472274E-4 7.655136117889096E-4 0.0 6.808286684847616 0.0 80-81 1.913784029472274E-4 7.655136117889096E-4 0.0 7.4806947036026985 0.0 82-83 1.913784029472274E-4 7.655136117889096E-4 0.0 8.14472991722884 0.0 84-85 1.913784029472274E-4 7.655136117889096E-4 0.0 8.803980670781302 0.0 86-87 1.913784029472274E-4 7.655136117889096E-4 0.0 9.501602794124683 0.0 88-89 1.913784029472274E-4 7.655136117889096E-4 0.0 10.210564087842688 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7100 0.0 30.472212 1 TACATGG 7175 0.0 29.889181 2 ATGGGCG 115 3.892832E-6 28.885567 5 ACATGGG 7205 0.0 27.790524 3 CATGGGA 5365 0.0 25.915392 4 GAGTACT 5045 0.0 24.455248 12-13 CATGGGG 1905 0.0 23.414925 4 TGCACCG 125 2.270278E-4 22.778334 5 ATGGGAT 1950 0.0 22.145601 5 ATGGGAG 2290 0.0 21.966005 5 GTACTTT 5700 0.0 21.853167 14-15 GTATCAA 14160 0.0 21.645458 1 AGTACTT 5175 0.0 21.548515 12-13 GTATAGG 305 2.1100277E-10 20.222786 1 CGGTATA 170 3.2160642E-7 19.536493 2 ACTTTTT 6680 0.0 19.395834 16-17 TATCAAC 15910 0.0 19.354055 2 CAACGCA 16150 0.0 19.305166 5 TCAACGC 16170 0.0 19.104286 4 AACGCAG 16420 0.0 19.015713 6 >>END_MODULE