FastQCFastQC Report
Fri 27 May 2016
SRR939627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045050
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63600.6085833213721832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47460.45414095019377065No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36510.34936127458016364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34570.3307975694942826No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30120.28821587483852446No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28660.27424525142337686No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18540.1774077795320798No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15290.14630878905315536No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA14850.14209846418831634No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14190.13578297689105784No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11490.10994689249318214No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11470.10975551409023492No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC11140.10659777044160566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG652.4669862E-729.9349664-65
GTACATG73300.027.8119011
TACATGG73500.027.4786822
ACATGGG73600.026.0968133
CATGGGA55450.023.7717764
CATGGGG17450.023.7406374
ATAGGCG1000.001741985823.5383035
ATGGGAT20250.022.3177975
ATGGGGA9050.021.3275225
GAGTACT53000.021.0613312-13
AGTACTT53650.020.89376412-13
GGTATCA95000.020.814811
GTATCAA120250.020.4768581
ATGGGAG21350.019.8449385
GTATAGG3353.8198777E-1119.6756921
GCGATTA756.580716E-419.45772664-65
GTACTTT59000.018.9991614-15
CGCTTAA800.001025716518.21455870-71
GTCGGGA2603.600744E-718.1072542
TCAACGC139300.017.607264
Produced by FastQC (version 0.11.5)