##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939627_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1045050 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.581544423711783 31.0 31.0 34.0 30.0 34.0 2 31.77410841586527 31.0 31.0 34.0 30.0 34.0 3 31.812194631835798 31.0 31.0 34.0 30.0 34.0 4 35.598981866896324 37.0 35.0 37.0 33.0 37.0 5 35.337352279795226 37.0 35.0 37.0 33.0 37.0 6 35.38324290703794 37.0 35.0 37.0 33.0 37.0 7 35.309104827520216 37.0 35.0 37.0 32.0 37.0 8 35.27966126022678 37.0 35.0 37.0 32.0 37.0 9 36.86832017606813 39.0 37.0 39.0 33.0 39.0 10-11 36.76283048657959 39.0 36.0 39.0 32.0 39.0 12-13 36.70626812114253 39.0 35.0 39.0 32.0 39.0 14-15 37.700373187885745 39.5 36.5 41.0 32.0 41.0 16-17 37.692907516386775 39.0 36.5 41.0 32.0 41.0 18-19 37.74111525764317 39.5 37.0 41.0 32.0 41.0 20-21 37.70602411367877 39.5 37.0 41.0 32.0 41.0 22-23 37.5747662791254 39.0 36.0 41.0 32.0 41.0 24-25 37.44857566623607 39.0 36.0 41.0 32.0 41.0 26-27 37.17614611741065 39.0 36.0 41.0 31.0 41.0 28-29 36.98102435290177 39.0 36.0 40.0 31.0 41.0 30-31 36.7265389215827 38.5 35.5 40.0 30.0 41.0 32-33 36.518101047796755 38.0 35.0 40.0 30.0 41.0 34-35 36.47650734414621 38.0 35.0 40.0 30.0 41.0 36-37 36.313764891631976 38.0 35.0 40.0 30.0 41.0 38-39 36.31007511602316 38.5 35.0 40.0 30.0 41.0 40-41 36.20148605329888 38.5 35.0 40.0 30.0 41.0 42-43 35.99240323429501 38.0 35.0 40.0 29.5 41.0 44-45 35.7219802880245 38.0 35.0 40.0 28.5 41.0 46-47 35.32169991866418 38.0 34.5 40.0 27.0 41.0 48-49 34.95627386249462 38.0 34.0 40.0 25.5 41.0 50-51 34.70413473039568 38.0 34.0 40.0 24.5 41.0 52-53 34.466903975886325 38.0 34.0 40.0 24.0 41.0 54-55 34.193825175828906 38.0 33.0 40.0 23.5 41.0 56-57 32.27487105880101 37.0 30.5 39.5 15.0 41.0 58-59 27.379684704081143 32.5 17.5 38.5 4.5 40.0 60-61 28.10920386584374 33.0 19.0 38.0 7.0 40.0 62-63 31.175081096598248 35.0 29.5 38.5 6.5 40.0 64-65 31.436082962537675 35.5 31.0 39.0 2.0 40.0 66-67 24.849689966987228 27.5 18.0 35.5 2.0 39.0 68-69 17.38493899813406 13.0 7.0 28.0 2.0 35.5 70-71 16.299771781254485 12.5 7.5 27.0 2.0 34.0 72-73 17.46085641835319 13.5 7.0 28.5 2.0 34.0 74-75 18.47521888904837 17.5 8.5 30.0 2.0 34.5 76-77 19.563173532366875 21.5 8.0 30.0 2.0 34.5 78-79 22.646378163724222 27.5 10.5 33.5 2.0 36.0 80-81 26.281289411989857 31.5 22.5 35.0 2.0 36.0 82-83 27.54895028945983 33.5 26.5 35.0 2.0 36.0 84-85 27.591910913353427 34.0 27.0 35.0 2.0 36.0 86-87 27.346565714559112 34.0 26.5 35.0 2.0 36.0 88-89 26.93752212812784 33.5 26.0 35.0 2.0 35.5 90-91 25.64796182000861 33.0 18.0 35.0 2.0 35.0 92-93 23.347375723649584 31.5 2.0 34.5 2.0 35.0 94-95 16.06394670111478 7.0 2.0 32.0 2.0 35.0 96-97 13.498449834936128 2.0 2.0 30.0 2.0 34.0 98-99 13.216347543179753 2.0 2.0 30.5 2.0 34.0 100-101 12.939279938758911 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4430999104347286 1101 2 0.5617407871093008 1101 3 0.5692780087393281 1101 4 0.3041266155500679 1101 5 0.6310446458192942 1101 6 0.6456787269816928 1101 7 0.5408783583245835 1101 8 0.6929548521851956 1101 9 0.7930551189735624 1101 10-11 0.8193999850248019 1101 12-13 0.6736142348178547 1101 14-15 1.0271604690508056 1101 16-17 0.8312346817587937 1101 18-19 0.8707185404961422 1101 20-21 0.6816941136055377 1101 22-23 0.6878283703850627 1101 24-25 0.6796117968695015 1101 26-27 0.7074979823195306 1101 28-29 0.795319485792561 1101 30-31 0.8695440675477286 1101 32-33 0.6444287933817776 1101 34-35 0.5454738384287126 1101 36-37 0.5075605111266981 1101 38-39 0.44534788022694727 1101 40-41 0.9635978956470623 1101 42-43 0.9788638723614227 1101 44-45 0.986048078439218 1101 46-47 0.9649748693575901 1101 48-49 1.160287245685609 1101 50-51 1.139595756344498 1101 52-53 0.8166547702368021 1101 54-55 0.5008865252028158 1101 56-57 -1.4539039051547213 1101 58-59 -9.750967015906618 1101 60-61 -9.818371531788369 1101 62-63 -3.262679598752417 1101 64-65 -4.468201760784794 1101 66-67 -9.411211995641663 1101 68-69 -4.242499507411166 1101 70-71 -3.8161003691640474 1101 72-73 -4.051197098783367 1101 74-75 -5.881171317582684 1101 76-77 -7.318363812910292 1101 78-79 -10.483451486389232 1101 80-81 -7.488257451548897 1101 82-83 -3.3220339274242257 1101 84-85 -2.3984067799768347 1101 86-87 -2.153241043577591 1101 88-89 -2.0356674724239774 1101 90-91 -8.521066496345941 1101 92-93 -16.808514786048327 1101 94-95 -11.462474415488574 1101 96-97 -9.729533674233409 1101 98-99 -9.83752125157645 1101 100-101 -9.725857374163194 1102 1 0.40749619526444647 1102 2 0.39503561328800885 1102 3 0.47144673168805085 1102 4 0.3466731707000079 1102 5 0.3134953951685233 1102 6 0.41792431021105614 1102 7 0.5175661269119871 1102 8 0.4419613675957592 1102 9 0.7226370853029067 1102 10-11 0.6657140801836832 1102 12-13 0.48374315677155266 1102 14-15 0.7444775215591548 1102 16-17 0.6031356259542306 1102 18-19 0.7493536158931491 1102 20-21 0.6044526121345655 1102 22-23 0.8098941252023053 1102 24-25 0.5985446095861064 1102 26-27 0.6594583815617128 1102 28-29 0.605209663387555 1102 30-31 0.6361849344207897 1102 32-33 0.7366322497839803 1102 34-35 0.6800216874190497 1102 36-37 0.6676828868641067 1102 38-39 0.6586563379383961 1102 40-41 0.37239755062295643 1102 42-43 0.5378086993102684 1102 44-45 0.9293790243289664 1102 46-47 0.676015255661369 1102 48-49 0.45223308922333416 1102 50-51 0.3802866517128578 1102 52-53 0.38326444631461953 1102 54-55 0.21596053595401798 1102 56-57 -2.7848363173969126 1102 58-59 -10.555462341138043 1102 60-61 -9.99197526236151 1102 62-63 -4.257592803625883 1102 64-65 -5.364783095596799 1102 66-67 -6.906926149331419 1102 68-69 -4.367580372435887 1102 70-71 -4.22656545857766 1102 72-73 -4.507268136782228 1102 74-75 -5.0085914922511385 1102 76-77 -7.326602418643237 1102 78-79 -9.893841486851318 1102 80-81 -5.453928323196848 1102 82-83 -3.018863248312048 1102 84-85 -2.419017734492183 1102 86-87 -2.2960332743740786 1102 88-89 -2.4130876470924285 1102 90-91 -8.285776229414346 1102 92-93 -13.525209948009422 1102 94-95 -10.514975070110202 1102 96-97 -9.020765210052764 1102 98-99 -9.342779403541083 1102 100-101 -9.229328794153028 1103 1 0.22882709772570564 1103 2 0.36841166829102434 1103 3 0.299324208479117 1103 4 0.30385286021959956 1103 5 0.40923932819255526 1103 6 0.3462680064971764 1103 7 0.2697124146165777 1103 8 0.24107087928979354 1103 9 0.48295241839134206 1103 10-11 0.45965231970621545 1103 12-13 0.43556563054670505 1103 14-15 0.6440617562652946 1103 16-17 0.6320889677230568 1103 18-19 0.551707502794109 1103 20-21 0.5257043751971011 1103 22-23 0.5963044845269252 1103 24-25 0.5828964375021357 1103 26-27 0.5640812335732974 1103 28-29 0.5288673548754304 1103 30-31 0.5264538496441205 1103 32-33 0.6542042575663984 1103 34-35 0.6998654948177929 1103 36-37 0.6087744890174278 1103 38-39 0.7463961938094457 1103 40-41 0.3939885473258613 1103 42-43 0.438720616479209 1103 44-45 0.3988428945856981 1103 46-47 0.5471240462571529 1103 48-49 0.38815398610628904 1103 50-51 0.22349823735801522 1103 52-53 0.27633442005575404 1103 54-55 0.12061003231904266 1103 56-57 -2.339555315841338 1103 58-59 -9.963599413297757 1103 60-61 -10.259305997020032 1103 62-63 -3.669892063933279 1103 64-65 -4.771762555634805 1103 66-67 -5.890946340082312 1103 68-69 -3.9390558346870748 1103 70-71 -3.7309243614198753 1103 72-73 -4.321301887808319 1103 74-75 -5.399613819085516 1103 76-77 -6.816839814163357 1103 78-79 -8.877368473613043 1103 80-81 -4.242914114386757 1103 82-83 -2.101536708721941 1103 84-85 -1.8533189948267932 1103 86-87 -1.4468902021327317 1103 88-89 -1.4934901015475894 1103 90-91 -4.450046038073875 1103 92-93 -9.456181797966565 1103 94-95 -8.249081429833916 1103 96-97 -7.726090565953298 1103 98-99 -8.01680841527367 1103 100-101 -8.17213924055366 1104 1 0.2725099693691284 1104 2 0.3530211676100592 1104 3 0.2395552250339925 1104 4 0.1094810322188664 1104 5 0.18618086588655558 1104 6 0.24334267294285183 1104 7 0.2721951854759155 1104 8 0.2789712219054721 1104 9 0.2588643511773938 1104 10-11 0.34379749232407875 1104 12-13 0.390723407035388 1104 14-15 0.4639637680128388 1104 16-17 0.29017391933580683 1104 18-19 0.38955310056346093 1104 20-21 0.2515511415261784 1104 22-23 0.3874796822654787 1104 24-25 0.2901948295606118 1104 26-27 0.5209206980782994 1104 28-29 0.44412459287237027 1104 30-31 0.41820972780922006 1104 32-33 0.1622263865382223 1104 34-35 0.3535411424853194 1104 36-37 0.18072545638565174 1104 38-39 0.2651366772884671 1104 40-41 0.24587109200694357 1104 42-43 0.08328551632710912 1104 44-45 0.09845273292098966 1104 46-47 0.021205626123105503 1104 48-49 0.13619962020479193 1104 50-51 0.007500102365931127 1104 52-53 0.022249274241097794 1104 54-55 0.1005938432467488 1104 56-57 -3.0400290178067095 1104 58-59 -10.094379326459372 1104 60-61 -10.348894180169165 1104 62-63 -4.270294446924542 1104 64-65 -4.831728670751332 1104 66-67 -6.324336343539443 1104 68-69 -4.145411259323762 1104 70-71 -4.284702802162764 1104 72-73 -4.351017708482953 1104 74-75 -5.063088291998955 1104 76-77 -6.483820803303956 1104 78-79 -9.596221460262498 1104 80-81 -4.886709321785176 1104 82-83 -2.6599998517960053 1104 84-85 -2.1978833924283983 1104 86-87 -2.0528620780142752 1104 88-89 -2.127134492753928 1104 90-91 -4.1309785922557865 1104 92-93 -6.1238763953868585 1104 94-95 -7.2530717766600965 1104 96-97 -7.94001023835478 1104 98-99 -8.192156827714982 1104 100-101 -8.357518767153042 1105 1 0.18745874208092417 1105 2 0.18254469708251975 1105 3 0.22938457058345918 1105 4 0.154571879403683 1105 5 0.22204254704956838 1105 6 0.056417238826412586 1105 7 0.06938568082779994 1105 8 0.07580059840798725 1105 9 0.24864468774016046 1105 10-11 0.33572777575079016 1105 12-13 0.2377470138904485 1105 14-15 0.4862112685545199 1105 16-17 0.475368562395623 1105 18-19 0.38621146149659324 1105 20-21 0.27529083674186694 1105 22-23 0.29364810809683206 1105 24-25 0.37493652640649344 1105 26-27 0.38406568213748216 1105 28-29 0.4556275179350493 1105 30-31 0.3782261329226628 1105 32-33 0.3971638613888757 1105 34-35 0.4881649598210842 1105 36-37 0.23392471144904903 1105 38-39 0.38448645700597694 1105 40-41 0.3153722836991477 1105 42-43 0.34971126513283934 1105 44-45 0.21080196580138022 1105 46-47 0.22040007314697618 1105 48-49 0.45042331785545286 1105 50-51 0.368987808848523 1105 52-53 0.4271112236638217 1105 54-55 0.3982163915077095 1105 56-57 1.3612101357307083 1105 58-59 -8.279161262262758 1105 60-61 -10.275725052735481 1105 62-63 -3.963760001345154 1105 64-65 -4.829614835772325 1105 66-67 -5.712679685023744 1105 68-69 -3.9472382627595497 1105 70-71 -3.8815419804626163 1105 72-73 -2.151469880870774 1105 74-75 -4.217812242432814 1105 76-77 -5.8576200831297935 1105 78-79 -8.649207913146807 1105 80-81 -4.046834578437501 1105 82-83 -1.8585971630082305 1105 84-85 -1.5052019817949969 1105 86-87 -1.3297551146110926 1105 88-89 -1.5216529665624954 1105 90-91 -4.007868017664109 1105 92-93 -5.685636746600736 1105 94-95 -7.843067752764347 1105 96-97 -7.787689017979423 1105 98-99 -8.447131239865083 1105 100-101 -8.26998226835505 1106 1 -0.040380252612003176 1106 2 0.18126854131197945 1106 3 0.07340796797764426 1106 4 0.20238976149122578 1106 5 0.1036709651948371 1106 6 0.2401836697838533 1106 7 0.10259465223901998 1106 8 -0.0794710456276988 1106 9 0.06046051640992545 1106 10-11 0.24454771125611785 1106 12-13 0.27877379508577604 1106 14-15 0.35246797469886104 1106 16-17 0.4579325870944757 1106 18-19 0.3362045926694961 1106 20-21 0.1819272082658756 1106 22-23 0.33902213380793 1106 24-25 0.30479857143708244 1106 26-27 0.1868960004172351 1106 28-29 0.24521907578503033 1106 30-31 0.2090613822972358 1106 32-33 0.09879105037561686 1106 34-35 0.44518237049018694 1106 36-37 0.4384838050530888 1106 38-39 0.3124649973440654 1106 40-41 0.23311807925393424 1106 42-43 0.2685108833706593 1106 44-45 0.33771619945717646 1106 46-47 0.16423295096861068 1106 48-49 0.23997872398389575 1106 50-51 0.44759249700377524 1106 52-53 0.45722380012471575 1106 54-55 0.45084787531896353 1106 56-57 1.7786757554435226 1106 58-59 -8.933706256695391 1106 60-61 -9.854494231223761 1106 62-63 -3.6705713472014416 1106 64-65 -5.151587490610147 1106 66-67 -6.384470858219412 1106 68-69 -3.7606031926975128 1106 70-71 -4.0495715670315295 1106 72-73 -4.367532452270424 1106 74-75 -5.213575260667742 1106 76-77 -6.68645614230293 1106 78-79 -9.681404636354767 1106 80-81 -4.702012227997173 1106 82-83 -2.348839813363238 1106 84-85 -1.8259382477559818 1106 86-87 -1.7237691125576724 1106 88-89 -1.6383416857793023 1106 90-91 -3.966033245492259 1106 92-93 -6.201068108942209 1106 94-95 -7.835078222302906 1106 96-97 -7.597185713712073 1106 98-99 -7.129648628843146 1106 100-101 -7.391743074104621 1107 1 0.05767934544759612 1107 2 0.02479888484232262 1107 3 0.03427817884785611 1107 4 0.1395494486509179 1107 5 0.11510547662934556 1107 6 0.0346347142348975 1107 7 -0.014225464581095082 1107 8 0.15652044036378499 1107 9 -0.020843314893909337 1107 10-11 0.07333941504781905 1107 12-13 -0.11321709690511739 1107 14-15 0.00971725694814296 1107 16-17 0.03928591844780982 1107 18-19 0.08559821706312931 1107 20-21 0.1586869350255995 1107 22-23 0.05508984987564247 1107 24-25 0.25840465004316115 1107 26-27 0.2611955747168153 1107 28-29 0.15765348821943803 1107 30-31 0.16228383551969472 1107 32-33 0.20253077911534945 1107 34-35 0.12829542860324494 1107 36-37 -0.10404911247982795 1107 38-39 0.16741747729654577 1107 40-41 -0.1275887814529284 1107 42-43 -0.13898589912612636 1107 44-45 -0.020070158329176024 1107 46-47 -0.0021119653763008728 1107 48-49 0.07784131184648402 1107 50-51 0.16722421663549625 1107 52-53 0.05209064499155858 1107 54-55 0.060515234986318944 1107 56-57 1.2880683898361482 1107 58-59 -8.74772219571133 1107 60-61 -10.373180499910031 1107 62-63 -3.840071891701985 1107 64-65 -4.843536307558971 1107 66-67 -6.444266918015472 1107 68-69 -3.9473668794611996 1107 70-71 -3.962760855220818 1107 72-73 -2.425732135470108 1107 74-75 -4.800039472131953 1107 76-77 -6.704226660073449 1107 78-79 -7.665218120168248 1107 80-81 -2.2752475512324963 1107 82-83 -1.0237358632592866 1107 84-85 -0.8281533749711087 1107 86-87 -0.9127231765117365 1107 88-89 -0.7576491800867977 1107 90-91 -0.11547389493290794 1107 92-93 -2.732921390795493 1107 94-95 -5.671096933321618 1107 96-97 -6.530496772023131 1107 98-99 -6.516095515290032 1107 100-101 -7.269539826901374 1108 1 -0.13007088881426654 1108 2 -0.011064476602431483 1108 3 -0.0766980542213993 1108 4 -0.0416201569372916 1108 5 -0.022768277523475433 1108 6 -0.22307463068374744 1108 7 -0.25385405304689357 1108 8 0.053040586883930985 1108 9 -0.182334924059937 1108 10-11 -0.20163076980607997 1108 12-13 -0.0027732771589725758 1108 14-15 -0.2079439578060871 1108 16-17 -0.0655569131392113 1108 18-19 -0.0027078567080636162 1108 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82-83 1.9581347890242071 2316 84-85 1.4381243075909786 2316 86-87 1.2667194756373341 2316 88-89 1.4598618257269607 2316 90-91 2.553709826544395 2316 92-93 4.701635184128058 2316 94-95 11.40442563279728 2316 96-97 13.403472546120499 2316 98-99 13.14354927417127 2316 100-101 12.245561661823967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 13.0 5 58.0 6 114.0 7 279.0 8 550.0 9 963.0 10 1752.0 11 2919.0 12 4429.0 13 6004.0 14 7266.0 15 10086.0 16 13435.0 17 18238.0 18 18528.0 19 16643.0 20 16800.0 21 16887.0 22 17026.0 23 14821.0 24 14794.0 25 17839.0 26 24878.0 27 36959.0 28 51710.0 29 69507.0 30 88212.0 31 98507.0 32 100356.0 33 93179.0 34 83745.0 35 76660.0 36 62352.0 37 41489.0 38 16679.0 39 1365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54242836086598 20.26358607224211 14.547577573170498 21.646407993721407 2 15.0364837772791 22.361564998540647 40.204596238223516 22.397354985956735 3 18.817257804919592 27.98393563536916 31.228290429320815 21.970516130390433 4 12.100952107554662 17.65207406344194 38.97172384096454 31.27524998803885 5 12.05125256358653 40.129568934123014 34.16939796446437 13.649780537826079 6 28.639969379455525 38.33740012439596 18.460934883498396 14.561695612650114 7 26.385723171140135 35.09573704607435 21.845366250418643 16.67317353236687 8 25.714909074206627 34.63878479811067 20.6197345908435 19.026571536839203 9 27.11640591359265 16.748193866322186 21.203483086933637 34.93191713315152 10-11 24.027080044017033 27.43514664370126 28.180852590785133 20.35692072149658 12-13 26.33424238074733 25.231998468972776 28.278312042486004 20.155447107793886 14-15 22.803459385804 25.999314859416998 28.08330008104945 23.113925673729547 16-17 20.709439739725372 29.452131476962823 28.379120616238456 21.459308167073345 18-19 20.61708140818345 28.143813421099594 31.15534829244767 20.083756878269284 20-21 22.112626190134442 26.86010238744558 30.449882780728192 20.577388641691783 22-23 21.43855730890572 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21.72973578843148 48-49 21.408001655435633 27.67940113083115 29.214798521604006 21.697798692129208 50-51 21.377315751734074 27.933580908870137 29.27777908170328 21.411324257692506 52-53 21.336083358913296 28.313882185954277 28.918654760530842 21.43137969460158 54-55 20.676885596537574 27.79973157473125 29.280667203900258 22.242715624830918 56-57 20.391796861468112 28.32261367266737 29.448466722863092 21.837122743001427 58-59 19.879961821946935 27.655604010154235 30.421227619866787 22.043206548032046 60-61 21.083391331157074 27.731553204966954 29.49226612848534 21.692789335390632 62-63 21.69704387418367 27.572501230861835 29.001784211380006 21.72867068357449 64-65 20.764729184759258 27.83141354284573 29.507372196656416 21.896485075738596 66-67 20.49189243767325 28.01109982181557 29.125081763386877 22.3719259771243 68-69 20.573444881666703 28.054527368870687 29.022388659000963 22.34963909046165 70-71 20.513461633115128 28.220898754737412 28.719446768715855 22.54619284343161 72-73 19.74059456253099 27.981130397133487 29.3793465424485 22.898928497887024 74-75 20.21359604448876 28.19092783223257 28.918377052878498 22.67709907040017 76-77 18.66343041599993 28.25278864536119 30.084803551139604 22.998977387499277 78-79 19.469551008816183 27.8711280976547 29.560079540300727 23.09924135322839 80-81 20.3170080551698 28.244028513587327 28.66313478084165 22.775828650401227 82-83 20.695691864281574 28.117326892787492 28.263205638794847 22.923775604136086 84-85 20.57734217822635 27.739791731954767 28.048246610799985 23.634619479018895 86-87 20.431359208761723 27.64458580990014 28.418523404004898 23.505531577333237 88-89 20.739027547990574 27.12891931383122 28.485171508704376 23.646881629473835 90-91 19.825462903672193 27.388097537016225 29.003241992508737 23.78319756680284 92-93 18.90730488873945 27.803777935225106 29.537737321821 23.751179854214453 94-95 18.150580918981895 28.153159091819084 28.859519854372294 24.83674013482672 96-97 18.108112614936722 28.227154395305813 28.099049117486075 25.56568387227139 98-99 18.300291948483107 27.68928721372425 28.503151247758208 25.507269590034436 100-101 18.793641561814205 27.49352577204051 28.567950530666902 25.14488213547838 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 327.0 1 248.5 2 176.0 3 206.5 4 253.5 5 317.5 6 402.5 7 538.5 8 739.0 9 929.5 10 1068.0 11 1191.0 12 1333.0 13 1506.0 14 1717.5 15 1874.5 16 2042.0 17 2247.0 18 2418.5 19 2602.5 20 2943.5 21 3343.5 22 3794.0 23 4386.5 24 5209.0 25 6315.5 26 7680.0 27 9378.5 28 11040.5 29 12836.5 30 14997.0 31 17595.0 32 20401.0 33 23164.0 34 25998.5 35 28564.5 36 30762.0 37 32401.5 38 33355.5 39 33995.5 40 34695.0 41 35587.0 42 36731.5 43 38116.0 44 39622.5 45 41053.5 46 42555.5 47 43971.0 48 45180.5 49 43362.5 50 38862.0 51 34938.5 52 31558.5 53 28766.5 54 26060.0 55 23177.0 56 20500.0 57 17910.0 58 15330.5 59 13073.5 60 10906.5 61 8723.5 62 6769.5 63 5069.5 64 3667.0 65 2684.0 66 1874.0 67 1282.0 68 885.5 69 568.0 70 413.5 71 305.0 72 220.0 73 173.0 74 122.5 75 73.0 76 45.5 77 26.0 78 13.0 79 13.5 80 12.0 81 5.5 82 2.0 83 1.5 84 1.0 85 1.0 86 1.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022008516338931153 2 0.00621979809578489 3 3.827568058944548E-4 4 0.0 5 0.1521458303430458 6 0.0 7 0.0 8 0.0021051624324195015 9 0.0 10-11 0.0 12-13 0.0 14-15 8.612028132625233E-4 16-17 0.0 18-19 1.4353380221042054E-4 20-21 0.0 22-23 2.870676044208411E-4 24-25 4.784460073680685E-5 26-27 1.913784029472274E-4 28-29 0.0037797234582077413 30-31 0.01430553562030525 32-33 0.002870676044208411 34-35 0.005310750681785561 36-37 0.11219558872781207 38-39 0.11123869671307593 40-41 0.12468302952011866 42-43 0.12712310415769582 44-45 0.12382182670685614 46-47 0.11564039998086216 48-49 0.11635806899191427 50-51 0.11420506195875796 52-53 0.08980431558298646 54-55 0.07765178699583752 56-57 0.5586335582029568 58-59 1.5990144012248217 60-61 0.5262427635041386 62-63 0.004832304674417492 64-65 0.11764987321180806 66-67 1.9126836036553276 68-69 2.888235012678819 70-71 2.79412468302952 72-73 2.645471508540261 74-75 2.015310272235778 76-77 1.4205540404765322 78-79 0.9426821683173054 80-81 0.05411224343332855 82-83 3.827568058944548E-4 84-85 2.3922300368403425E-4 86-87 4.3060140663126167E-4 88-89 9.56892014736137E-5 90-91 0.2521888904837089 92-93 0.39591407109707666 94-95 2.016075785847567 96-97 2.447347016889144 98-99 1.4260083249605282 100-101 0.9554088321132961 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1045050.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.691263716748956 #Duplication Level Percentage of deduplicated Percentage of total 1 76.54541992242925 40.33274907442009 2 12.879567019340266 13.572813247472007 3 4.375728749168533 6.916880324261977 4 1.9824262737706184 4.178261823610385 5 1.071088147625924 2.821849402522085 6 0.676392565973476 2.1383987441854164 7 0.4421280020762077 1.6307398207769757 8 0.3240658254201111 1.3660350295037604 9 0.2402270393005121 1.1392079651709388 >10 1.3031790692029113 12.649348250268424 >50 0.09921184243149081 3.6251434257336035 >100 0.05476285710174174 5.107281951164567 >500 0.0032640109644635437 1.1959636244160603 >1k 0.0023573412521125593 2.7176474689834627 >5k 1.8133394247019688E-4 0.6076798475102775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6360 0.6085833213721832 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4746 0.45414095019377065 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3651 0.34936127458016364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3457 0.3307975694942826 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3012 0.28821587483852446 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2866 0.27424525142337686 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1854 0.1774077795320798 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1529 0.14630878905315536 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1485 0.14209846418831634 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1419 0.13578297689105784 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1149 0.10994689249318214 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1147 0.10975551409023492 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1114 0.10659777044160566 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.56892014736137E-5 0.0 0.0 0.0 0.0 8 9.56892014736137E-5 0.0 0.0 0.0 0.0 9 9.56892014736137E-5 0.0 0.0 0.0 0.0 10-11 9.56892014736137E-5 9.56892014736137E-5 0.0 0.0 0.0 12-13 2.8706760442084113E-4 9.56892014736137E-5 0.0 0.0 0.0 14-15 2.8706760442084113E-4 9.56892014736137E-5 0.0 0.0 0.0 16-17 3.3491220515764796E-4 9.56892014736137E-5 0.0 0.0 0.0 18-19 3.827568058944548E-4 9.56892014736137E-5 0.0 4.784460073680685E-5 0.0 20-21 3.827568058944548E-4 9.56892014736137E-5 0.0 1.913784029472274E-4 0.0 22-23 3.827568058944548E-4 9.56892014736137E-5 0.0 6.21979809578489E-4 0.0 24-25 3.827568058944548E-4 9.56892014736137E-5 0.0 0.001913784029472274 0.0 26-27 3.827568058944548E-4 9.56892014736137E-5 0.0 0.0030620544471556387 0.0 28-29 4.306014066312616E-4 9.56892014736137E-5 0.0 0.00971245394957179 0.0 30-31 4.784460073680685E-4 9.56892014736137E-5 0.0 0.02416152337208746 0.0 32-33 4.784460073680685E-4 9.56892014736137E-5 0.0 0.056121716664274436 0.0 34-35 4.784460073680685E-4 9.56892014736137E-5 0.0 0.1018611549686618 0.0 36-37 4.784460073680685E-4 9.56892014736137E-5 0.0 0.17625950911439645 0.0 38-39 4.784460073680685E-4 9.56892014736137E-5 0.0 0.2908473278790489 0.0 40-41 4.784460073680685E-4 9.56892014736137E-5 0.0 0.43136692024305057 0.0 42-43 6.21979809578489E-4 9.56892014736137E-5 0.0 0.5698770393761063 0.0 44-45 6.698244103152959E-4 9.56892014736137E-5 0.0 0.7261375053825176 0.0 46-47 6.698244103152959E-4 9.56892014736137E-5 0.0 0.9052676905411224 0.0 48-49 7.655136117889096E-4 9.56892014736137E-5 0.0 1.0791349696186785 0.0 50-51 7.655136117889096E-4 9.56892014736137E-5 0.0 1.2493660590402373 0.0 52-53 7.655136117889096E-4 9.56892014736137E-5 0.0 1.415147600593273 0.0 54-55 7.655136117889096E-4 9.56892014736137E-5 0.0 1.5886321228649347 0.0 56-57 7.655136117889096E-4 9.56892014736137E-5 0.0 1.7290560260274628 0.0 58-59 7.655136117889096E-4 9.56892014736137E-5 0.0 1.826324099325391 0.0 60-61 7.655136117889096E-4 9.56892014736137E-5 0.0 1.9157935027032198 0.0 62-63 7.655136117889096E-4 9.56892014736137E-5 0.0 1.995263384527056 0.0 64-65 8.133582125257165E-4 9.56892014736137E-5 0.0 2.0640160757858474 0.0 66-67 8.612028132625233E-4 9.56892014736137E-5 0.0 2.1210946844648584 0.0 68-69 8.612028132625233E-4 9.56892014736137E-5 0.0 2.2041529113439546 0.0 70-71 8.612028132625233E-4 9.56892014736137E-5 0.0 2.338069948806277 0.0 72-73 8.612028132625233E-4 9.56892014736137E-5 0.0 2.5298311085593994 0.0 74-75 8.612028132625233E-4 9.56892014736137E-5 0.0 2.7666140376058563 0.0 76-77 8.612028132625233E-4 9.56892014736137E-5 0.0 3.1154968661786517 0.0 78-79 8.612028132625233E-4 9.56892014736137E-5 0.0 3.6642744366298263 0.0 80-81 9.56892014736137E-4 9.56892014736137E-5 0.0 4.353475910243529 0.0 82-83 9.56892014736137E-4 9.56892014736137E-5 0.0 5.03205588249366 0.0 84-85 9.56892014736137E-4 9.56892014736137E-5 0.0 5.556432706569064 0.0 86-87 9.56892014736137E-4 9.56892014736137E-5 0.0 5.9477058513946695 0.0 88-89 9.56892014736137E-4 9.56892014736137E-5 9.56892014736137E-5 6.299650734414621 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCGG 65 2.4669862E-7 29.93496 64-65 GTACATG 7330 0.0 27.811901 1 TACATGG 7350 0.0 27.478682 2 ACATGGG 7360 0.0 26.096813 3 CATGGGA 5545 0.0 23.771776 4 CATGGGG 1745 0.0 23.740637 4 ATAGGCG 100 0.0017419858 23.538303 5 ATGGGAT 2025 0.0 22.317797 5 ATGGGGA 905 0.0 21.327522 5 GAGTACT 5300 0.0 21.06133 12-13 AGTACTT 5365 0.0 20.893764 12-13 GGTATCA 9500 0.0 20.81481 1 GTATCAA 12025 0.0 20.476858 1 ATGGGAG 2135 0.0 19.844938 5 GTATAGG 335 3.8198777E-11 19.675692 1 GCGATTA 75 6.580716E-4 19.457726 64-65 GTACTTT 5900 0.0 18.99916 14-15 CGCTTAA 80 0.0010257165 18.214558 70-71 GTCGGGA 260 3.600744E-7 18.107254 2 TCAACGC 13930 0.0 17.60726 4 >>END_MODULE