FastQCFastQC Report
Fri 27 May 2016
SRR939626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053598
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27400.2600612377775964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18840.17881582918722322No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14950.14189472645164475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.12784762309723444No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11680.11085822106723818No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11350.10772609667064668No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG58500.034.5138471
TATCACG600.004059980631.4907972
TACATGG70600.027.6994132
ACATGGG69750.026.1407493
GGGCGTA1551.4916004E-624.35919
GAGTACT47000.023.44781112-13
ATGGGAG20400.023.1351765
CATGGGA53100.023.0376834
ATACCGT1050.00227964622.4741696
AGTACTT49950.022.06300512-13
CATGGGG18500.021.701054
GTACTAT2803.4389322E-421.41861
CATATAG3101.2732926E-1121.3324763
ATGGGAT18500.020.91925
GTACTTT53400.020.64053214-15
GTATCAA119950.020.4156741
GTTATAG5951.7735147E-920.1586821
ACCGTGG1908.717574E-619.8728438
CCTCTAG5151.7314414E-719.408441
ATGGGTA2502.3234315E-718.8783045
Produced by FastQC (version 0.11.5)