FastQCFastQC Report
Fri 27 May 2016
SRR939626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053598
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63660.6042152699606491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47620.45197504171420216No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35880.3405473434839474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35280.33485257185378103No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29290.27799976841262036No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28420.2697423495488792No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18760.178056526303201No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14790.1403761206836004No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA13900.13192887609885365No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13510.1282272745392455No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12250.1162682541158962No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11890.11285139113779638No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC11480.10895996385718272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG68650.028.7257231
TACATGG68950.028.2532582
ACATGGG66650.027.7381043
ATGGGAT18050.026.1284525
CATGGGA50750.025.7415454
GTCGGGA2103.7107384E-822.4612262
CATGGGG16050.022.3320944
GAGTACT47900.021.30726612-13
ATGGGAG18450.020.9608255
AGTACTT48900.020.82333412-13
GGGATAG3252.5465852E-1120.30727
TGCACCG1405.090774E-420.2122235
TACACTG9450.019.962695
GTACTTT51800.019.70305314-15
GTACACG1951.0950353E-519.3548851
AGGACGT2201.4397665E-619.2934865
GTATCAA125700.019.0286481
TGGGATA6500.018.1314286
TTAGGGC4700.018.0619873
GGTATCA100250.017.8827571
Produced by FastQC (version 0.11.5)