##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939626_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1053598 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.505691924244353 31.0 31.0 34.0 30.0 34.0 2 31.694027513340004 31.0 31.0 34.0 30.0 34.0 3 31.739527789536428 31.0 31.0 34.0 30.0 34.0 4 35.54157752767185 37.0 35.0 37.0 33.0 37.0 5 35.31271319801291 37.0 35.0 37.0 33.0 37.0 6 35.33128100091306 37.0 35.0 37.0 32.0 37.0 7 35.23505454642093 37.0 35.0 37.0 32.0 37.0 8 35.20736087198343 37.0 35.0 37.0 32.0 37.0 9 36.78104267472034 39.0 35.0 39.0 32.0 39.0 10-11 36.668266739306645 39.0 35.0 39.0 32.0 39.0 12-13 36.6045645492873 39.0 35.0 39.0 32.0 39.0 14-15 37.60616762750119 39.0 36.0 41.0 32.0 41.0 16-17 37.59251061600345 39.0 36.0 41.0 32.0 41.0 18-19 37.626442438197486 39.0 36.5 41.0 32.0 41.0 20-21 37.597677672129215 39.0 36.0 41.0 32.0 41.0 22-23 37.46732387495041 39.0 36.0 41.0 32.0 41.0 24-25 37.32942640361884 39.0 36.0 41.0 31.5 41.0 26-27 37.033477664156536 39.0 36.0 40.5 31.0 41.0 28-29 36.84734784993897 39.0 36.0 40.0 30.5 41.0 30-31 36.586553410313996 38.0 35.0 40.0 30.0 41.0 32-33 36.38805170472989 38.0 35.0 40.0 30.0 41.0 34-35 36.318830331872306 38.0 35.0 40.0 30.0 41.0 36-37 36.18919739786902 38.0 35.0 40.0 30.0 41.0 38-39 36.18586643102967 38.0 35.0 40.0 30.0 41.0 40-41 36.060789788894816 38.0 35.0 40.0 30.0 41.0 42-43 35.85840140167312 38.0 35.0 40.0 29.0 41.0 44-45 35.59520946319184 38.0 35.0 40.0 27.5 41.0 46-47 35.20124278899542 38.0 34.0 40.0 26.5 41.0 48-49 34.84123925823701 38.0 34.0 40.0 25.0 41.0 50-51 34.58332067828527 38.0 34.0 40.0 24.0 41.0 52-53 34.358575566772146 38.0 33.0 40.0 24.0 41.0 54-55 34.1001278476231 38.0 33.0 40.0 23.0 41.0 56-57 33.33300129650968 37.5 32.0 40.0 18.5 41.0 58-59 30.31806818160247 35.5 26.0 39.0 7.5 40.5 60-61 30.08282855510356 34.5 25.5 39.0 9.5 40.0 62-63 31.892954428539156 35.5 30.5 39.0 13.0 40.0 64-65 31.96101216972697 36.0 31.0 39.0 8.0 40.0 66-67 27.141094136473303 32.0 18.5 37.0 2.0 39.5 68-69 19.739733275879416 20.0 8.0 32.5 2.0 37.0 70-71 17.807385739152885 15.5 7.5 29.0 2.0 35.5 72-73 19.56594782829884 20.0 7.5 31.0 2.0 36.0 74-75 20.99309651309133 23.5 9.0 32.5 2.0 36.0 76-77 22.717767118008958 27.5 11.5 33.0 2.0 35.5 78-79 24.875712083735923 30.5 13.0 34.5 2.0 37.0 80-81 26.469625986381903 32.0 22.5 35.0 2.0 37.0 82-83 27.608776782036415 33.0 26.5 35.0 2.0 36.5 84-85 27.66330706778107 34.0 27.0 35.0 2.0 36.0 86-87 27.389082458394945 34.0 26.5 35.0 2.0 36.0 88-89 26.95103445526662 33.0 26.0 35.0 2.0 35.5 90-91 25.154803350044325 32.5 16.0 35.0 2.0 35.0 92-93 23.589803226657605 31.5 2.0 34.0 2.0 35.0 94-95 19.896844432126862 28.0 2.0 34.0 2.0 35.0 96-97 17.22959515868481 18.5 2.0 33.0 2.0 35.0 98-99 17.54695291752642 21.5 2.0 33.0 2.0 35.0 100-101 16.806270513041976 17.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.13895808765926532 1101 2 0.20117840548860855 1101 3 0.14786259669401147 1101 4 0.22138404238722842 1101 5 0.2830063679792332 1101 6 0.28300366212245365 1101 7 0.2144292138703605 1101 8 0.3263447568049074 1101 9 0.3658771728794932 1101 10-11 0.3542195551830716 1101 12-13 0.3113718887995347 1101 14-15 0.5378845831058712 1101 16-17 0.45363717520044844 1101 18-19 0.37813478468049766 1101 20-21 0.3774154412119728 1101 22-23 0.5632824721538441 1101 24-25 0.4142256251169414 1101 26-27 0.3343642457823961 1101 28-29 0.22292883178666045 1101 30-31 0.3203224683734547 1101 32-33 0.1624641746626665 1101 34-35 0.16838475086127147 1101 36-37 0.02479689793813833 1101 38-39 -0.05346964095447504 1101 40-41 -0.18911406402304465 1101 42-43 -0.13106600417567194 1101 44-45 5.12812853813216E-4 1101 46-47 -0.19910114021939762 1101 48-49 -0.15878929263162433 1101 50-51 0.06370789666589616 1101 52-53 -0.1808882140549528 1101 54-55 -0.2232019105587142 1101 56-57 -3.5255004258885343 1101 58-59 -10.095414022435339 1101 60-61 -11.620412853263257 1101 62-63 -4.120716856041337 1101 64-65 -1.3848614314168373 1101 66-67 -2.295785080093683 1101 68-69 -4.398376104765749 1101 70-71 -4.454133382961894 1101 72-73 -3.551273513088409 1101 74-75 -6.249065438531243 1101 76-77 -4.88743149024679 1101 78-79 1.16463820598684 1101 80-81 1.5814449167769133 1101 82-83 0.2312070888226465 1101 84-85 -0.15158695462553595 1101 86-87 -0.24103646928109512 1101 88-89 -0.518771606320449 1101 90-91 1.1045415829635097 1101 92-93 0.6618826160519653 1101 94-95 -8.08082282548025 1101 96-97 -9.060546849963481 1101 98-99 -8.00425492448002 1101 100-101 -9.306724006090269 1102 1 0.2672503777175237 1102 2 0.3532388442612522 1102 3 0.41414779317655714 1102 4 0.2956969904979445 1102 5 0.32916704590812174 1102 6 0.4192596338595749 1102 7 0.3343924105184044 1102 8 0.4634716538120571 1102 9 0.38164313240071834 1102 10-11 0.3323915297418907 1102 12-13 0.354722747148422 1102 14-15 0.4701572392476905 1102 16-17 0.3354068166954889 1102 18-19 0.30408852158896593 1102 20-21 0.32004375111716854 1102 22-23 0.421342955169159 1102 24-25 0.12238946393923555 1102 26-27 0.12158442109809187 1102 28-29 0.22849357725608854 1102 30-31 0.12402713704087631 1102 32-33 0.23825556552950644 1102 34-35 0.45739306766505194 1102 36-37 0.12699502155708586 1102 38-39 -0.104626464035384 1102 40-41 -0.10752975018188948 1102 42-43 -0.11821596472594109 1102 44-45 -0.2645815182665672 1102 46-47 -0.2001646030608768 1102 48-49 -0.2583504708304787 1102 50-51 -0.0033013230161955676 1102 52-53 -0.1943662537111308 1102 54-55 -0.3596078158089284 1102 56-57 -3.722810880842527 1102 58-59 -10.093785236511724 1102 60-61 -8.105859061133373 1102 62-63 -2.4437109217875914 1102 64-65 -0.9290508523375607 1102 66-67 -1.8847902835239658 1102 68-69 -4.762236065225904 1102 70-71 -4.597477195254967 1102 72-73 -3.4814188338928744 1102 74-75 -4.261050287872642 1102 76-77 -4.201773245255428 1102 78-79 1.6694176439573774 1102 80-81 2.0286265354197965 1102 82-83 0.528691974623289 1102 84-85 0.3270667735163322 1102 86-87 0.1337473356054275 1102 88-89 0.3003229591222514 1102 90-91 1.7902522637190081 1102 92-93 1.6426657278032302 1102 94-95 -8.546546221908553 1102 96-97 -9.690492095555395 1102 98-99 -8.701722686726434 1102 100-101 -9.647983332896988 1103 1 0.13564293680973094 1103 2 0.08750900128019623 1103 3 0.13363307436047123 1103 4 0.1777924527012118 1103 5 0.2751809849064344 1103 6 0.16176107036599063 1103 7 0.1725053687834901 1103 8 0.26739771379743615 1103 9 0.36627661804933354 1103 10-11 0.16649008776307284 1103 12-13 0.2680105292216055 1103 14-15 0.27326517462639544 1103 16-17 0.37652920704216086 1103 18-19 0.16465045944589463 1103 20-21 0.2348000922669229 1103 22-23 0.17649385674795326 1103 24-25 0.12193955403848378 1103 26-27 0.013103756279022605 1103 28-29 0.14295519028666348 1103 30-31 0.094497646639077 1103 32-33 -0.05808691323561277 1103 34-35 0.012338363155393495 1103 36-37 0.12041601761304577 1103 38-39 0.24148441471565008 1103 40-41 0.2809133113944071 1103 42-43 0.3128880148854378 1103 44-45 0.05362478359913325 1103 46-47 0.06220596477935203 1103 48-49 0.20759670482200931 1103 50-51 0.17942659289638385 1103 52-53 0.17862586211623466 1103 54-55 0.024696934629844236 1103 56-57 0.5417165964958528 1103 58-59 -8.601071744296217 1103 60-61 -7.816149528594057 1103 62-63 -2.1657858233820164 1103 64-65 -0.06951655403674195 1103 66-67 -1.9050374017161964 1103 68-69 -3.577423944629725 1103 70-71 -3.9864225454693667 1103 72-73 -3.0641737209463322 1103 74-75 -3.90654161215566 1103 76-77 -0.5983983346706232 1103 78-79 3.334804288942898 1103 80-81 2.71545836449312 1103 82-83 1.0451799411947071 1103 84-85 0.5694587619148272 1103 86-87 0.562604501915736 1103 88-89 0.4572039225783122 1103 90-91 1.9163435199878442 1103 92-93 1.335727499533565 1103 94-95 -7.093382976146737 1103 96-97 -8.783520641967256 1103 98-99 -7.713273761858652 1103 100-101 -9.74743609235741 1104 1 0.18971235367579453 1104 2 0.16273454656400688 1104 3 0.1731185737862866 1104 4 0.18231065571309557 1104 5 0.21828173806829199 1104 6 0.29488982225274185 1104 7 0.25082267998413244 1104 8 0.30927827561546906 1104 9 0.23552065247739762 1104 10-11 0.27158478029559063 1104 12-13 0.2441770467409654 1104 14-15 0.34554413256686445 1104 16-17 0.2895783795627054 1104 18-19 0.2697332457991166 1104 20-21 0.45354080256653617 1104 22-23 0.401718441856012 1104 24-25 0.25793731106792706 1104 26-27 0.31289751052779735 1104 28-29 0.1838421622380082 1104 30-31 0.2735128135752376 1104 32-33 0.5343568876777098 1104 34-35 0.3766670658811364 1104 36-37 0.3101528910503575 1104 38-39 0.07025550497601074 1104 40-41 0.07835271470278116 1104 42-43 0.06662060209819032 1104 44-45 0.07928106372465038 1104 46-47 0.17862919488739237 1104 48-49 0.055184935371727306 1104 50-51 0.08322043932204082 1104 52-53 0.10106977825063979 1104 54-55 0.19942856550756005 1104 56-57 0.5928793693297933 1104 58-59 -7.777242486588886 1104 60-61 -7.198011808665804 1104 62-63 -1.8849816569873994 1104 64-65 -0.23425698012433216 1104 66-67 -1.398261249409277 1104 68-69 -3.3619270920858355 1104 70-71 -4.061629271308465 1104 72-73 -3.2334388792409072 1104 74-75 -3.8025338385727814 1104 76-77 -0.6341391486692203 1104 78-79 3.1868846057240816 1104 80-81 2.7889886234717274 1104 82-83 1.3186088056140512 1104 84-85 0.9822867099424393 1104 86-87 0.9542636472056891 1104 88-89 0.6873323172796795 1104 90-91 2.0886689504346343 1104 92-93 2.0884484455755263 1104 94-95 -6.185080912537014 1104 96-97 -7.237108050606533 1104 98-99 -5.2741081929929265 1104 100-101 -4.8667690390599745 1105 1 0.3050903682156054 1105 2 0.29793985601366657 1105 3 0.1545078879162638 1105 4 0.10615779469866027 1105 5 0.23244932195901669 1105 6 0.32606476147949337 1105 7 0.26922741949631757 1105 8 0.2849171097760177 1105 9 0.4718601161473188 1105 10-11 0.36642803604816976 1105 12-13 0.3658017194197427 1105 14-15 0.43827186156586606 1105 16-17 0.33264900924750407 1105 18-19 0.4156157297562686 1105 20-21 0.31041723704012725 1105 22-23 0.4613189364323773 1105 24-25 0.4080226262535689 1105 26-27 0.3470865128397378 1105 28-29 0.36395102395722745 1105 30-31 0.37553024884133634 1105 32-33 0.37762789554104614 1105 34-35 0.2633649143519108 1105 36-37 0.36852228406542764 1105 38-39 0.5726891044378064 1105 40-41 0.5602443091166052 1105 42-43 0.6132853771094275 1105 44-45 0.4771755291832491 1105 46-47 0.5418136900251511 1105 48-49 0.38924365754322565 1105 50-51 0.4208389824269929 1105 52-53 0.6124509841962364 1105 54-55 0.4526159667772518 1105 56-57 1.0300127180709637 1105 58-59 -7.4367776885421755 1105 60-61 -7.142918869651098 1105 62-63 -1.4980504026444095 1105 64-65 0.3239175266150731 1105 66-67 -1.3904429154542903 1105 68-69 -3.393886534383098 1105 70-71 -3.631953087631775 1105 72-73 -2.6457701843839985 1105 74-75 -3.3872666536840583 1105 76-77 0.18113230769952082 1105 78-79 4.109861812038851 1105 80-81 3.5632794243055805 1105 82-83 2.022504471663641 1105 84-85 1.423943250913176 1105 86-87 1.2562370709723574 1105 88-89 1.6801302968696312 1105 90-91 3.1198473221006857 1105 92-93 2.854170733776858 1105 94-95 -5.633439863308968 1105 96-97 -7.8280028570207705 1105 98-99 -5.166920359998642 1105 100-101 -4.252758279506924 1106 1 0.16213370696716467 1106 2 0.11785311752621652 1106 3 0.17409793245828453 1106 4 0.20617210256165208 1106 5 0.09080616944423525 1106 6 0.1201183197981095 1106 7 0.17777202836391126 1106 8 0.038577928983272614 1106 9 -0.027256783784430638 1106 10-11 0.11103150887144153 1106 12-13 0.16474809777124477 1106 14-15 0.09479753714327899 1106 16-17 0.3307831231803178 1106 18-19 0.21987243627386022 1106 20-21 0.2031997411037949 1106 22-23 0.11473323819694059 1106 24-25 0.18674214288235902 1106 26-27 0.08684407665631966 1106 28-29 0.2750405559962914 1106 30-31 0.17453292042427648 1106 32-33 0.08575218161386289 1106 34-35 0.0875423881462396 1106 36-37 0.2529710443242763 1106 38-39 0.20115025858978441 1106 40-41 0.05522013821035188 1106 42-43 0.16480189330898298 1106 44-45 0.0976489083486598 1106 46-47 -0.04180052307756199 1106 48-49 -0.1925328161080202 1106 50-51 -0.17970542003208578 1106 52-53 -0.17099364554675844 1106 54-55 -0.3812216600877534 1106 56-57 0.5182626787829818 1106 58-59 -7.963516973477404 1106 60-61 -7.2616412947635105 1106 62-63 -2.4333847258003978 1106 64-65 -0.9277742022673436 1106 66-67 0.16255598917324576 1106 68-69 -2.9023270007725195 1106 70-71 -2.458555861480754 1106 72-73 -1.5033788010012898 1106 74-75 -1.2403776756642166 1106 76-77 1.9096382618815184 1106 78-79 3.931514299847848 1106 80-81 2.9101688714356797 1106 82-83 1.31938559208659 1106 84-85 0.7375985815303601 1106 86-87 0.7671227746025657 1106 88-89 0.6300281481100747 1106 90-91 1.9675412933301537 1106 92-93 1.87177644191053 1106 94-95 -6.365885170916297 1106 96-97 -7.740458672697967 1106 98-99 -5.402249522941744 1106 100-101 -4.724945904847321 1107 1 0.07591011310018558 1107 2 0.04553726884773468 1107 3 0.04043888437262311 1107 4 0.08432288529205323 1107 5 -0.07903789499966507 1107 6 0.0474109723662437 1107 7 0.07642498265318665 1107 8 0.12402539374266297 1107 9 0.07769311887508934 1107 10-11 0.026977565990577546 1107 12-13 0.1668055649040454 1107 14-15 0.2687492858758702 1107 16-17 0.21410581049492095 1107 18-19 0.18436853616785243 1107 20-21 0.2827819745393114 1107 22-23 0.15044586132253102 1107 24-25 0.24061443186796083 1107 26-27 0.11394945087342023 1107 28-29 0.19649239821542608 1107 30-31 0.24393504153857748 1107 32-33 0.27547339329105824 1107 34-35 0.011189828637284904 1107 36-37 0.16173504536522643 1107 38-39 0.15805258003239686 1107 40-41 0.007206212149434066 1107 42-43 0.13790409737827503 1107 44-45 -0.022136736544425162 1107 46-47 0.04821137451612856 1107 48-49 -0.06990021975391869 1107 50-51 0.035493736833764444 1107 52-53 0.04295508109019153 1107 54-55 -7.0738488320643E-4 1107 56-57 0.5844587837901187 1107 58-59 -8.37793214073437 1107 60-61 -7.602219644500867 1107 62-63 -2.104088866453001 1107 64-65 -0.3755556711737178 1107 66-67 0.49019465642906823 1107 68-69 -2.848111673405537 1107 70-71 -3.019117971269825 1107 72-73 -1.325438801810236 1107 74-75 -0.9450636028485029 1107 76-77 2.0057996662096826 1107 78-79 4.00176276037249 1107 80-81 2.861075461969957 1107 82-83 1.3580481926609416 1107 84-85 0.9320044230275535 1107 86-87 0.8936882926439438 1107 88-89 0.7269492737033367 1107 90-91 2.218198179396385 1107 92-93 2.009606897766183 1107 94-95 -5.954640533037379 1107 96-97 -7.584369722349624 1107 98-99 -5.289560079137079 1107 100-101 -4.209540848637104 1108 1 0.08628796979309428 1108 2 -0.04330698983418202 1108 3 -0.1200363715640762 1108 4 -0.09704338096357645 1108 5 -0.12056532463434877 1108 6 -0.06096405327470933 1108 7 -0.12289722335603415 1108 8 0.035387717474762326 1108 9 -0.10682862101437252 1108 10-11 -0.14950928183819912 1108 12-13 -0.01326737905538522 1108 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-0.04970303017750766 2312 90-91 1.5453621800807191 2312 92-93 3.01830036550189 2312 94-95 5.4854757456127 2312 96-97 0.7455926033100013 2312 98-99 -0.4196206511604714 2312 100-101 0.10083139752309123 2313 1 -0.27934253511828544 2313 2 -0.3221826286757512 2313 3 -0.31972900268517535 2313 4 -0.3212646285622611 2313 5 -0.4458500395569658 2313 6 -0.33015757243089183 2313 7 -0.5732252526430699 2313 8 -0.46441220278331485 2313 9 -0.48699172949963554 2313 10-11 -0.5184925722800457 2313 12-13 -0.4218816818572222 2313 14-15 -0.6322554927276158 2313 16-17 -0.6037051166995155 2313 18-19 -0.6083142045338548 2313 20-21 -0.5114535397510735 2313 22-23 -0.5130590535529151 2313 24-25 -0.680218098974926 2313 26-27 -0.8016099001086445 2313 28-29 -0.6179033719028553 2313 30-31 -0.6615863349899413 2313 32-33 -0.6724612688453178 2313 34-35 -0.5828814325142986 2313 36-37 -0.8851777587483767 2313 38-39 -0.8365285199548893 2313 40-41 -0.8004682613865484 2313 42-43 -0.639647367999558 2313 44-45 -0.8237432921342958 2313 46-47 -0.9446381630676797 2313 48-49 -0.9442225847083989 2313 50-51 -0.8689378273866737 2313 52-53 -0.7240885441074028 2313 54-55 -0.6590841132659335 2313 56-57 0.1886207112207572 2313 58-59 3.134622322626644 2313 60-61 3.020882995541289 2313 62-63 0.5772847549289786 2313 64-65 0.06322878292079892 2313 66-67 -2.1302225174721237 2313 68-69 -4.849410072664217 2313 70-71 -3.809151948412474 2313 72-73 -2.893810987794211 2313 74-75 -3.42002553754698 2313 76-77 0.2403400663041637 2313 78-79 2.630084071490465 2313 80-81 1.9723071788149902 2313 82-83 0.6059541468372238 2313 84-85 0.32595140414933965 2313 86-87 0.17291099736985416 2313 88-89 0.27977773455694077 2313 90-91 1.967853502179242 2313 92-93 3.5454322516190437 2313 94-95 5.808808419485469 2313 96-97 0.7175722439639465 2313 98-99 2.179840869315779 2313 100-101 3.4356138654643154 2314 1 -0.40737159223690966 2314 2 -0.43968696143138075 2314 3 -0.4313216130321642 2314 4 -0.3767666750978975 2314 5 -0.4531556186712393 2314 6 -0.44133866317908144 2314 7 -0.4663039059857468 2314 8 -0.4522222252767705 2314 9 -0.4293303113057476 2314 10-11 -0.6117213049029218 2314 12-13 -0.5245879025880811 2314 14-15 -0.7389260926759889 2314 16-17 -0.7342751967172134 2314 18-19 -0.5175730267618093 2314 20-21 -0.5973405562339806 2314 22-23 -0.7172944604632008 2314 24-25 -0.6506052813931547 2314 26-27 -0.9880223784448674 2314 28-29 -0.9843881242904828 2314 30-31 -0.6487665407497403 2314 32-33 -0.7808535399467615 2314 34-35 -0.6803020601185636 2314 36-37 -0.6884418909066383 2314 38-39 -0.894859579359732 2314 40-41 -0.7452206490114648 2314 42-43 -0.7927522022486144 2314 44-45 -0.629461054588667 2314 46-47 -0.7428047621310583 2314 48-49 -0.828004803816782 2314 50-51 -0.8492505232825422 2314 52-53 -0.9466037548452633 2314 54-55 -0.6315403247781575 2314 56-57 0.029511968273006062 2314 58-59 2.996249963763848 2314 60-61 2.905368042925037 2314 62-63 0.16769664327294365 2314 64-65 -0.5278995772694728 2314 66-67 -2.0920555496635806 2314 68-69 -5.065954930512959 2314 70-71 -3.6672053427116733 2314 72-73 -3.1164408782768973 2314 74-75 -4.031856357267468 2314 76-77 0.48566437767257753 2314 78-79 2.9662952149692074 2314 80-81 2.0897683683868777 2314 82-83 0.41915706729889024 2314 84-85 0.2724309941047238 2314 86-87 0.4281394349966128 2314 88-89 0.42208607925991615 2314 90-91 1.8031494201703175 2314 92-93 3.486077555649427 2314 94-95 6.287042130168015 2314 96-97 4.0363428475997765 2314 98-99 6.260530054020233 2314 100-101 6.567871323335183 2315 1 -0.5739602744327463 2315 2 -0.4850600709492845 2315 3 -0.4364978322834503 2315 4 -0.4264586877436969 2315 5 -0.508613351601106 2315 6 -0.5507380493592038 2315 7 -0.5727188469894671 2315 8 -0.7161970937549285 2315 9 -0.8171311800117138 2315 10-11 -0.6569181797778683 2315 12-13 -0.7958191747713315 2315 14-15 -0.8223635763223385 2315 16-17 -0.9710068059780781 2315 18-19 -0.8953162074815921 2315 20-21 -0.834069087160465 2315 22-23 -0.809185050302851 2315 24-25 -0.779474362910527 2315 26-27 -0.6642031505526944 2315 28-29 -0.9241389639061879 2315 30-31 -0.8375607879584592 2315 32-33 -1.0240281030541567 2315 34-35 -0.8367191559563452 2315 36-37 -0.9623736319202081 2315 38-39 -1.4486357721373437 2315 40-41 -1.1497014725274752 2315 42-43 -1.18191523391544 2315 44-45 -1.378387485475443 2315 46-47 -0.8968553884181887 2315 48-49 -1.067281426022035 2315 50-51 -1.193676121479868 2315 52-53 -1.2265982754100762 2315 54-55 -0.9454965693333506 2315 56-57 -0.33840132120432287 2315 58-59 2.75976385166938 2315 60-61 2.7447936565140374 2315 62-63 0.25678364008070886 2315 64-65 -0.5916364905004592 2315 66-67 -2.5355473351282996 2315 68-69 -5.515262459195338 2315 70-71 -3.7911991121629 2315 72-73 -2.9852568893096496 2315 74-75 -0.696925881449328 2315 76-77 1.366518215278127 2315 78-79 3.2234569598922604 2315 80-81 2.067585890881805 2315 82-83 0.12180766711234625 2315 84-85 -0.14336116907162477 2315 86-87 -0.1248286950076043 2315 88-89 -0.14101361875548335 2315 90-91 1.3426375239471255 2315 92-93 2.8006225316538114 2315 94-95 5.7603098744569365 2315 96-97 4.2116601469154595 2315 98-99 5.9923948166344765 2315 100-101 6.160562280112291 2316 1 -0.4568602656147398 2316 2 -0.4056932669736213 2316 3 -0.4760765204156705 2316 4 -0.3734986711154491 2316 5 -0.3336514579966803 2316 6 -0.4785972887429537 2316 7 -0.45425362143739534 2316 8 -0.48040511058473356 2316 9 -0.5546867649606284 2316 10-11 -0.6503542744077038 2316 12-13 -0.499434873346587 2316 14-15 -0.9209079749971139 2316 16-17 -0.8210455422314311 2316 18-19 -0.48666526836364454 2316 20-21 -0.555815241878733 2316 22-23 -0.7208782024890894 2316 24-25 -0.7694556876274063 2316 26-27 -0.5629928788320484 2316 28-29 -0.6067331591369012 2316 30-31 -0.5654693137133435 2316 32-33 -0.541592474519085 2316 34-35 -0.401498453790893 2316 36-37 -0.43007137576964993 2316 38-39 -0.8587448320116238 2316 40-41 -0.6509824457837183 2316 42-43 -0.6822892119711881 2316 44-45 -0.6314737885881954 2316 46-47 -0.5552409999471095 2316 48-49 -0.6170771946374813 2316 50-51 -0.8145938557554686 2316 52-53 -0.6954360618379027 2316 54-55 -0.5460344678758631 2316 56-57 0.2928405693767857 2316 58-59 3.111803173690472 2316 60-61 3.199251789867528 2316 62-63 0.8455117052835952 2316 64-65 0.08076424391370551 2316 66-67 -2.025523935911842 2316 68-69 -3.2414845407493225 2316 70-71 -3.1239569129514813 2316 72-73 -0.5728890019786057 2316 74-75 5.665187228410467 2316 76-77 5.6981414847438785 2316 78-79 4.883204638866353 2316 80-81 3.1954100156944243 2316 82-83 1.5208477638585016 2316 84-85 0.9123521133691987 2316 86-87 0.7003755363769493 2316 88-89 0.6198663870392096 2316 90-91 2.241505984448743 2316 92-93 3.963712393463016 2316 94-95 6.513276504591854 2316 96-97 3.704167988644798 2316 98-99 5.872234486337057 2316 100-101 6.05022379454233 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 19.0 5 47.0 6 115.0 7 258.0 8 598.0 9 1066.0 10 1945.0 11 2957.0 12 4339.0 13 5723.0 14 7208.0 15 9448.0 16 12662.0 17 16669.0 18 16543.0 19 14724.0 20 14795.0 21 15374.0 22 15392.0 23 15680.0 24 16801.0 25 18148.0 26 22442.0 27 31558.0 28 44513.0 29 58758.0 30 71475.0 31 79611.0 32 84130.0 33 85424.0 34 87155.0 35 93115.0 36 94638.0 37 75460.0 38 32418.0 39 2383.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40643118014463 20.323315330231445 14.613461788582383 21.65679170104155 2 15.08178265397076 22.389110960707683 40.11702356893408 22.41208281638748 3 18.721294764506734 28.002547463593764 31.335605549370392 21.940552222529107 4 12.130717787998838 17.718237885797052 38.92290987644244 31.228134449761676 5 12.04217868101874 40.041065616663296 34.28456648897525 13.632189213342716 6 28.664348261860784 38.34270756018899 18.50639427941207 14.48654989853815 7 26.246158401971147 35.11007044432507 21.88054647028563 16.763224683418155 8 25.68553251385492 34.69628330376795 20.618130081995297 19.000054100381835 9 27.037636745703768 16.8023287819453 21.09419342102016 35.06584105133077 10-11 23.96108382893665 27.472290190376214 28.23975558040163 20.3268704002855 12-13 26.29038779496544 25.246583611586203 28.36741337777786 20.09561521567049 14-15 22.781308549593106 26.045896237070448 28.05335831449306 23.119436898843386 16-17 20.629737338149845 29.550407271084417 28.356403485959543 21.463451904806195 18-19 20.60137102342699 28.106976843513976 31.221748446387004 20.06990368667203 20-21 22.11094743915611 26.941490018014463 30.427639384281292 20.51992315854814 22-23 21.414476868786767 27.216310205600237 30.244599932801698 21.1246129928113 24-25 21.33862441646304 27.208708457175018 30.74763441222517 20.705032714136767 26-27 21.435161105395238 27.015193679550798 31.04145232138315 20.508192893670817 28-29 20.637414767118713 27.592484320187094 31.08821887955778 20.681882033136418 30-31 20.913667231268132 27.501137824612226 30.943324232318396 20.641870711801246 32-33 20.82540767317805 27.615230556030145 30.544307762401047 21.01505400839076 34-35 21.377795305410295 27.258062908697962 30.412752017061916 20.951389768829827 36-37 21.68130243798638 26.899642053745136 30.77734564992704 20.64170985834144 38-39 21.43839269642349 27.10885689737633 30.805575331906272 20.647175074293905 40-41 21.03495511036931 27.216006945994703 30.98225218558497 20.766785758051018 42-43 20.85537671743651 27.60324093557363 30.27613880658409 21.265243540405773 44-45 21.05893663632339 27.70267959438431 29.776453465726537 21.46193030356576 46-47 21.28134051379776 27.783039525169784 29.222948100593634 21.712671860438824 48-49 21.41035742663462 27.73572171854689 29.17607644276505 21.677844412053442 50-51 21.38452295863243 28.0181038024159 29.18796467573915 21.409408563212523 52-53 21.348204695229835 28.289144406001853 28.977881951600327 21.384768947167977 54-55 20.71624598788293 27.79238599889845 29.296051507036637 22.19531650618198 56-57 21.214261155002724 28.365354477292975 28.649441608744297 21.77094275896 58-59 20.276351657196994 27.594680412233156 30.165855924883743 21.963112005686106 60-61 20.561260323937727 27.674509962644546 30.149493237210695 21.614736476207028 62-63 21.659559826778192 27.59447113958593 29.028605327163792 21.71736370647209 64-65 21.110048055795218 27.908713921373774 29.180915879104035 21.800322143726977 66-67 20.828435807954957 27.890467504579284 29.040446443221168 22.240650244244588 68-69 20.075391681065398 28.07577262382097 29.70872456089545 22.14011113421818 70-71 20.93703847648659 28.259424795958026 28.3793237465993 22.42421298095608 72-73 20.33680233457019 27.947298237476808 29.0080975572047 22.7078018707483 74-75 20.379010469963564 28.0892352547893 29.00193845489812 22.529815820349018 76-77 19.2914872724526 28.055629655763898 29.825668503395704 22.8272145683878 78-79 20.550128684374666 27.7938682957302 28.814323694771065 22.84167932512407 80-81 20.41642197274652 28.349632282601416 28.58548794539239 22.648457799259674 82-83 20.60342835231514 28.24658206438613 28.237517873303492 22.91247170999524 84-85 20.5650693463998 27.711604399254924 28.079822511182034 23.64350374316324 86-87 20.4847831379448 27.692890318716724 28.391125723660515 23.431200819677965 88-89 20.733154738882735 27.213048626254334 28.462529571302138 23.5912670635608 90-91 20.242413796551244 27.455883536249466 28.57801268680132 23.72368998039797 92-93 20.064718311531067 27.798532798366608 28.51864865506517 23.618100235037154 94-95 19.064227409354277 27.793869789805214 28.669641479766707 24.47226132107381 96-97 18.73163572220523 27.901109600422263 28.07267839342905 25.29457628394346 98-99 19.388989022262084 27.274468282236658 28.258147338378524 25.07839535712273 100-101 18.732705059882612 27.383492119854953 28.966298312481563 24.917504507780876 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 382.0 1 286.0 2 214.0 3 263.5 4 286.5 5 334.5 6 444.5 7 653.5 8 908.5 9 1113.5 10 1230.0 11 1292.5 12 1376.5 13 1507.0 14 1677.0 15 1861.0 16 2037.0 17 2219.5 18 2395.0 19 2599.0 20 2916.5 21 3248.5 22 3726.0 23 4320.0 24 5020.5 25 6107.0 26 7569.0 27 9236.5 28 10951.0 29 12839.0 30 14988.0 31 17566.5 32 20366.0 33 23094.5 34 25708.0 35 28209.0 36 30803.0 37 32715.5 38 33340.0 39 33573.5 40 34052.5 41 34975.0 42 36460.0 43 37751.5 44 39016.0 45 40358.5 46 41879.0 47 44256.5 48 46941.5 49 45095.5 50 39560.0 51 35247.0 52 31821.5 53 29121.0 54 26574.5 55 23798.0 56 21103.0 57 18670.0 58 16193.5 59 13768.0 60 11626.5 61 9393.5 62 7390.5 63 5604.5 64 4002.0 65 2899.5 66 2092.5 67 1474.0 68 976.0 69 662.0 70 450.5 71 316.5 72 262.0 73 196.5 74 143.5 75 106.0 76 69.0 77 43.0 78 22.5 79 14.0 80 10.0 81 4.5 82 2.5 83 2.5 84 0.5 85 0.5 86 0.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024772256591223597 2 0.012813236167874274 3 9.49128605027724E-5 4 0.0 5 0.06159844646629929 6 0.0 7 0.0 8 9.49128605027724E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 3.796514420110896E-4 16-17 0.0 18-19 5.220207327652482E-4 20-21 0.0 22-23 0.0 24-25 1.4236929075415862E-4 26-27 1.898257210055448E-4 28-29 0.0013287800470388137 30-31 0.004982925176395552 32-33 0.005504945909160799 34-35 0.0022304522218151517 36-37 0.08788930882556725 38-39 0.08100812643911624 40-41 0.08589613875500902 42-43 0.08912317601210329 44-45 0.09263495185070587 46-47 0.08798422168607002 48-49 0.07939460781056912 50-51 0.07407948762241386 52-53 0.06235774935032147 54-55 0.05106311895049156 56-57 0.07137447109808485 58-59 0.7155955117606525 60-61 0.3369881112150934 62-63 0.003369406547848421 64-65 0.0029422986755859447 66-67 1.0824811740341194 68-69 2.3321988082741236 70-71 2.315683970546641 72-73 1.7765789228908937 74-75 1.2658053640952243 76-77 0.7324425444998947 78-79 0.5580401633260503 80-81 0.039056642096890845 82-83 1.4236929075415862E-4 84-85 9.965850352791103E-4 86-87 0.0012813236167874273 88-89 4.74564302513862E-5 90-91 0.32759173802531893 92-93 0.054385069068088585 94-95 0.9440982234210771 96-97 1.6399993166274045 98-99 0.632214563808967 100-101 0.5661552128990375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1053598.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.63628138629313 #Duplication Level Percentage of deduplicated Percentage of total 1 76.36464365554852 40.19550871417456 2 13.06405914639781 13.75286986553943 3 4.398175876383773 6.94510869047228 4 1.979371421506721 4.167470044416592 5 1.0382335730243306 2.732437724720259 6 0.6802586725726563 2.1483772143000346 7 0.45079106373033623 1.6609575693855465 8 0.3138379172587619 1.3215408738016299 9 0.24207107901803343 1.1467549287609125 >10 1.3061333341041366 12.689384223325364 >50 0.10371451525676634 3.7953678013517878 >100 0.052748448675947676 4.916750346445354 >500 0.003612906983155803 1.3256749904461966 >1k 0.002167744189893482 2.5964859391847868 >5k 1.8064534915779014E-4 0.6053110736752609 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6366 0.6042152699606491 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4762 0.45197504171420216 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3588 0.3405473434839474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3528 0.33485257185378103 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2929 0.27799976841262036 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2842 0.2697423495488792 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1876 0.178056526303201 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1479 0.1403761206836004 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1390 0.13192887609885365 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1351 0.1282272745392455 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1225 0.1162682541158962 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1189 0.11285139113779638 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1148 0.10895996385718272 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 9.49128605027724E-5 0.0 0.0 0.0 3 0.0 9.49128605027724E-5 0.0 0.0 0.0 4 0.0 9.49128605027724E-5 0.0 9.49128605027724E-5 0.0 5 0.0 9.49128605027724E-5 0.0 9.49128605027724E-5 0.0 6 0.0 9.49128605027724E-5 0.0 9.49128605027724E-5 0.0 7 0.0 9.49128605027724E-5 0.0 9.49128605027724E-5 0.0 8 0.0 9.49128605027724E-5 0.0 9.49128605027724E-5 0.0 9 0.0 9.49128605027724E-5 0.0 9.49128605027724E-5 0.0 10-11 0.0 2.8473858150831723E-4 0.0 9.49128605027724E-5 0.0 12-13 0.0 2.8473858150831723E-4 0.0 9.49128605027724E-5 0.0 14-15 0.0 2.8473858150831723E-4 0.0 9.49128605027724E-5 0.0 16-17 0.0 2.8473858150831723E-4 0.0 9.49128605027724E-5 0.0 18-19 0.0 2.8473858150831723E-4 0.0 9.49128605027724E-5 0.0 20-21 0.0 5.694771630166345E-4 0.0 2.37282151256931E-4 0.0 22-23 0.0 5.694771630166345E-4 0.0 8.542157445249516E-4 0.0 24-25 0.0 5.694771630166345E-4 0.0 0.001613518628547131 0.0 26-27 0.0 5.694771630166345E-4 0.0 0.002989755105837331 0.0 28-29 0.0 5.694771630166345E-4 0.0 0.007165920967959316 0.0 30-31 0.0 5.694771630166345E-4 0.0 0.020691003589604386 0.0 32-33 0.0 5.694771630166345E-4 0.0 0.05248681185803314 0.0 34-35 0.0 5.694771630166345E-4 0.0 0.0997534163884138 0.0 36-37 0.0 5.694771630166345E-4 0.0 0.1713177132075042 0.0 38-39 9.49128605027724E-5 6.643900235194069E-4 0.0 0.28117934923946325 0.0 40-41 9.49128605027724E-5 6.643900235194069E-4 0.0 0.4141522668038474 0.0 42-43 9.49128605027724E-5 6.643900235194069E-4 0.0 0.5450371014371705 0.0 44-45 9.49128605027724E-5 6.643900235194069E-4 0.0 0.6886877158081166 0.0 46-47 9.49128605027724E-5 7.11846453770793E-4 0.0 0.8610020140508998 0.0 48-49 9.49128605027724E-5 7.593028840221792E-4 0.0 1.05134975578921 0.0 50-51 9.49128605027724E-5 7.593028840221792E-4 0.0 1.2530395843576012 0.0 52-53 9.49128605027724E-5 7.593028840221792E-4 0.0 1.441156873874096 0.0 54-55 9.49128605027724E-5 9.49128605027724E-4 0.0 1.6467381297231012 0.0 56-57 9.49128605027724E-5 9.49128605027724E-4 0.0 1.8382248257874445 0.0 58-59 9.49128605027724E-5 9.49128605027724E-4 0.0 1.990654879754897 0.0 60-61 9.49128605027724E-5 9.49128605027724E-4 0.0 2.1278988760419058 0.0 62-63 1.4236929075415862E-4 9.49128605027724E-4 0.0 2.244024760867048 0.0 64-65 1.898257210055448E-4 0.0010440414655304964 0.0 2.3594387992384194 0.0 66-67 1.898257210055448E-4 0.0010440414655304964 0.0 2.458717651324319 0.0 68-69 1.898257210055448E-4 0.0010440414655304964 0.0 2.580158656337616 0.0 70-71 2.8473858150831723E-4 0.0010440414655304964 0.0 2.782228136348019 0.0 72-73 2.8473858150831723E-4 0.0010440414655304964 0.0 3.054675502421227 0.0 74-75 2.8473858150831723E-4 0.0010440414655304964 0.0 3.3924703729505943 0.0 76-77 2.8473858150831723E-4 0.0010440414655304964 0.0 3.809375112709022 0.0 78-79 2.8473858150831723E-4 0.0010440414655304964 0.0 4.345158210247172 0.0 80-81 3.796514420110896E-4 0.0010440414655304964 0.0 5.0016230099145975 0.0 82-83 3.796514420110896E-4 0.0010440414655304964 0.0 5.6443254448091205 0.0 84-85 3.796514420110896E-4 0.0012338671865360412 0.0 6.2250497817953345 0.0 86-87 3.796514420110896E-4 0.001423692907541586 0.0 6.74222995867494 0.0 88-89 3.796514420110896E-4 0.001423692907541586 0.0 7.229417671635671 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6865 0.0 28.725723 1 TACATGG 6895 0.0 28.253258 2 ACATGGG 6665 0.0 27.738104 3 ATGGGAT 1805 0.0 26.128452 5 CATGGGA 5075 0.0 25.741545 4 GTCGGGA 210 3.7107384E-8 22.461226 2 CATGGGG 1605 0.0 22.332094 4 GAGTACT 4790 0.0 21.307266 12-13 ATGGGAG 1845 0.0 20.960825 5 AGTACTT 4890 0.0 20.823334 12-13 GGGATAG 325 2.5465852E-11 20.3072 7 TGCACCG 140 5.090774E-4 20.212223 5 TACACTG 945 0.0 19.96269 5 GTACTTT 5180 0.0 19.703053 14-15 GTACACG 195 1.0950353E-5 19.354885 1 AGGACGT 220 1.4397665E-6 19.293486 5 GTATCAA 12570 0.0 19.028648 1 TGGGATA 650 0.0 18.131428 6 TTAGGGC 470 0.0 18.061987 3 GGTATCA 10025 0.0 17.882757 1 >>END_MODULE