FastQCFastQC Report
Fri 27 May 2016
SRR939625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811070
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA98260.5425521929025383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92080.5084287189341108No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81350.44918197529637177No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA76380.42173963458066227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64940.3585725565549648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46040.2542143594670554No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43970.242784652166951No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT37380.20639732312942075No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA33360.1842005002567543No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33270.1837035564610976No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG32050.1769672072310844No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT29550.16316321290728686No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28620.15802812701883417No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG25350.139972502443307No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24830.1371012716239571No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC24710.13643867989641484No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19560.10800245158939191No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19290.10651162020242178No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG18400.10159739822314985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97750.027.0445371
TACATGG96100.026.5705172
ACATGGG96250.025.1484133
CATGGGG25150.022.6443774
GAGTACT71900.022.34327912-13
CATGGGA74800.021.6990954
AGTACTT77300.020.75173212-13
TAGCCCT12850.020.6824514
TCGCGCG600.00477601619.78408454-55
ATGGGAT30850.019.6922765
GTACTTT81500.019.56612214-15
GTATAGG6000.018.9845851
ATGGGAG27200.018.496015
CTTTGCG5150.018.4316372
ATAGCCC7400.017.317023
ACTTTTT96900.016.99717916-17
TTTGCGT5050.016.9169583
ATGGGGG14950.016.8258025
TACTTTT95500.016.67294314-15
AGTACAT70150.015.8317632
Produced by FastQC (version 0.11.5)