FastQCFastQC Report
Fri 27 May 2016
SRR939625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811070
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA98070.5415030893339297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97620.5390183703556461No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT88640.48943442274456533No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA85220.4705505585096103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75230.41538979719171537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55110.30429525087379283No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT53900.29761411762107487No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47940.2647053951531415No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG38570.21296802442754836No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA37670.20799858647098124No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT37100.2048512757651554No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT34290.18933558614520699No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33100.18276488484707934No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC31440.1735990326160778No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG26200.14466586051339816No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24620.1359417361007581No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23740.13108273009878138No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG22240.12280033350450287No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21340.11783089554793574No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC20350.11236451379571191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG99150.025.9705122
GTACATG101400.025.6777631
ACATGGG98150.024.602373
GTCGGGA3650.021.9251212
CATGGGA75900.021.4577524
CGGGAGT4200.021.2938524
GAGTACT72750.020.67030312-13
AGTACTT77400.020.03562412-13
TTCGCGA1001.0482054E-519.45560364-65
TAGCCCT11200.019.3325734
CATGGGG25600.019.3063094
TAGCGCG650.00660643218.70383868-69
ATGGGAG29200.018.2156835
GTACTTT81700.018.20436514-15
CCCGGAC1902.00059E-417.3418063
GCTTATA17800.016.9289471
CTTTGCG5900.016.7553392
ACTTTTT93500.016.40927716-17
TACTTTT94900.016.2664614-15
TTGCGTT6500.015.9315934
Produced by FastQC (version 0.11.5)