FastQCFastQC Report
Fri 27 May 2016
SRR939624_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939624_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803881
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41610.23066931798716214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38090.21115583566765211No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33590.18620962247509676No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32780.18171930410043677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26440.14657286151359208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19370.10737958878662174No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18970.10516214761395015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG80350.032.1001431
TACATGG94350.026.5860232
ACATGGG94450.025.1074453
GAGTACT67150.023.86308112-13
CATGGGA74750.021.8627784
AGTACTT75600.021.72651912-13
CATGGGG23750.021.6772064
GTACTTT78050.020.38158614-15
ACGCGGA1951.0915821E-519.363427
ATGGGAT28150.019.280135
GTATAGG4902.0660082E-618.5780051
AACGCGG1801.2955494E-418.3549086
GGCGCGA800.001228379417.73212494-95
GTATAGC8602.5465852E-1117.6419031
ACTTTTT94600.017.56778116-17
CAACGCG1901.960824E-417.3874135
GTGTAGC11650.017.3642791
GTTATAG11150.017.2357981
TACTTTT94900.017.18552614-15
ATGGGAG30100.017.0903325
Produced by FastQC (version 0.11.5)