FastQCFastQC Report
Fri 27 May 2016
SRR939624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803881
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA97770.5419980586302533No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97040.5379512284901277No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT89670.4970948748836536No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA83320.46189299626749214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75530.4187083294297129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54710.3032905163921567No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT52910.2933120311151345No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46790.2593851811732592No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG38240.21198737610740398No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA37370.20716444155684327No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35930.19918165333522556No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT33150.183770437185158No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32680.18116494380726889No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC30540.16930163353347588No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25050.13886725343855832No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG24940.13825745711607362No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23890.1324366740378107No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22040.1221810086142046No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG21480.11907659097246438No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19320.10710240864003778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG96950.025.799631
TACATGG97200.025.194992
ACATGGG95500.024.3547883
CATGGGA71800.022.99774
GCTTACG2450.022.43135360-61
ATGGGAT27100.021.9352235
GAGTACT74850.021.16407412-13
AGTACTT78950.020.57258812-13
CATTCCG3303.092282E-1120.0016549
ATGGGAG27200.018.9059475
GTACTTT83850.018.80809814-15
CATGGGG25250.018.4975684
CGAATCG800.001062190618.12052772-73
TAGGACA8900.016.9629274
TACTTTT95600.016.86630814-15
ACTTTTT96000.016.62414216-17
GTGTAGC10300.016.4949381
TAGCCCT12300.016.4931724
GGTATCG2351.0913936E-1116.48590562-63
GTATAGC9000.016.255661
Produced by FastQC (version 0.11.5)