##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939624_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1803881 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.470282130584003 31.0 31.0 34.0 30.0 34.0 2 31.654756605341483 31.0 31.0 34.0 30.0 34.0 3 31.707426376795365 31.0 31.0 34.0 30.0 34.0 4 35.49687812000902 37.0 35.0 37.0 33.0 37.0 5 35.26395920795219 37.0 35.0 37.0 33.0 37.0 6 35.28613084787744 37.0 35.0 37.0 32.0 37.0 7 35.19693205926555 37.0 35.0 37.0 32.0 37.0 8 35.16960708605501 37.0 35.0 37.0 32.0 37.0 9 36.740528338621004 39.0 35.0 39.0 32.0 39.0 10-11 36.62341944950914 39.0 35.0 39.0 32.0 39.0 12-13 36.56615209096387 39.0 35.0 39.0 32.0 39.0 14-15 37.55606467388924 39.0 36.0 41.0 32.0 41.0 16-17 37.53300439441404 39.0 36.0 41.0 32.0 41.0 18-19 37.57589109259425 39.0 36.0 41.0 32.0 41.0 20-21 37.528059223418836 39.0 36.0 41.0 32.0 41.0 22-23 37.39620407332856 39.0 36.0 41.0 32.0 41.0 24-25 37.259028727504756 39.0 36.0 41.0 31.0 41.0 26-27 36.925911687079136 39.0 36.0 40.0 30.5 41.0 28-29 36.73572258923953 39.0 35.5 40.0 30.0 41.0 30-31 36.50696442836306 38.0 35.0 40.0 30.0 41.0 32-33 36.31599867175274 38.0 35.0 40.0 30.0 41.0 34-35 36.2705508290181 38.0 35.0 40.0 30.0 41.0 36-37 36.15232379519492 38.0 35.0 40.0 30.0 41.0 38-39 36.14902036220793 38.0 35.0 40.0 30.0 41.0 40-41 36.03724386475605 38.0 35.0 40.0 30.0 41.0 42-43 35.862113964280354 38.0 35.0 40.0 29.0 41.0 44-45 35.62281990885208 38.0 35.0 40.0 28.0 41.0 46-47 35.273929932185105 38.0 34.0 40.0 27.0 41.0 48-49 34.93908938560803 38.0 34.0 40.0 26.0 41.0 50-51 34.69622802169323 38.0 34.0 40.0 24.5 41.0 52-53 34.44517792470789 38.0 33.0 40.0 24.0 41.0 54-55 34.20816838804777 38.0 33.0 40.0 23.5 41.0 56-57 33.44030315746993 37.5 32.0 40.0 19.0 41.0 58-59 30.360586701672673 35.5 26.0 39.0 7.5 40.5 60-61 30.109939624620473 34.5 25.5 39.0 10.0 40.0 62-63 31.94986254636531 35.5 30.5 39.0 13.5 40.0 64-65 32.006092419621915 36.0 31.0 39.0 10.5 40.0 66-67 27.08660770860162 31.5 18.5 37.0 2.0 39.5 68-69 19.619624021761965 19.5 8.0 32.0 2.0 37.0 70-71 17.701742243529367 15.5 7.5 29.0 2.0 35.5 72-73 19.39253254510691 19.5 7.5 31.0 2.0 35.5 74-75 20.84410002655386 23.5 9.0 32.5 2.0 36.0 76-77 22.650583935414808 27.5 11.5 33.0 2.0 36.0 78-79 24.81848553202789 30.5 13.0 34.5 2.0 37.0 80-81 26.41579544326926 32.0 22.5 35.0 2.0 37.0 82-83 27.53168363101557 33.0 26.0 35.0 2.0 36.5 84-85 27.56437924674632 34.0 26.0 35.0 2.0 36.0 86-87 27.276061724692482 33.5 26.0 35.0 2.0 36.0 88-89 26.80203793930974 33.0 25.5 35.0 2.0 35.5 90-91 24.960496562689002 32.0 15.0 34.5 2.0 35.0 92-93 23.372343574770177 31.0 2.0 34.0 2.0 35.0 94-95 19.62295406404303 27.0 2.0 34.0 2.0 35.0 96-97 16.95711718234185 16.5 2.0 33.0 2.0 35.0 98-99 17.231961531830535 20.0 2.0 33.0 2.0 35.0 100-101 16.452374907213944 10.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.14533591654333122 1101 2 0.1543044356686636 1101 3 0.11529410305526255 1101 4 0.1927829182684846 1101 5 0.28986028209109804 1101 6 0.256389858579567 1101 7 0.2355466736943015 1101 8 0.3035449225178226 1101 9 0.2765184930136968 1101 10-11 0.27616624077485596 1101 12-13 0.2372131711326162 1101 14-15 0.4161317243235203 1101 16-17 0.3510209464293723 1101 18-19 0.3659521052469117 1101 20-21 0.4456990528538256 1101 22-23 0.3354341850656084 1101 24-25 0.405655516455802 1101 26-27 0.4992575883457633 1101 28-29 0.39186829886324404 1101 30-31 0.3093347600087526 1101 32-33 0.36837836892527065 1101 34-35 0.2570844568228665 1101 36-37 0.2587195904245121 1101 38-39 0.22865799882363547 1101 40-41 0.31990488674427553 1101 42-43 0.28245142464908923 1101 44-45 0.36979252681977925 1101 46-47 0.3269328247482193 1101 48-49 0.18581212027952887 1101 50-51 0.27183343488589173 1101 52-53 0.16349204838362397 1101 54-55 0.1306567348177552 1101 56-57 -3.1326677926371644 1101 58-59 -10.195778524086432 1101 60-61 -11.704265950912923 1101 62-63 -3.820172992891333 1101 64-65 -0.9150276700742808 1101 66-67 -1.760961236694687 1101 68-69 -4.375099978397788 1101 70-71 -4.48851585726341 1101 72-73 -3.3823375439604355 1101 74-75 -5.837021354268639 1101 76-77 -4.4076359426903835 1101 78-79 1.6133542611676326 1101 80-81 2.184495591961987 1101 82-83 0.7496521363880113 1101 84-85 0.40069760627294215 1101 86-87 0.5016314810950568 1101 88-89 0.3051109280778235 1101 90-91 1.799973189422687 1101 92-93 1.5081033282663014 1101 94-95 -8.40012420493608 1101 96-97 -9.502890939144919 1101 98-99 -8.480803411095142 1101 100-101 -9.328970148437417 1102 1 0.3686164240007521 1102 2 0.3711489202294622 1102 3 0.3250001678034913 1102 4 0.20033314397707613 1102 5 0.3817003022647185 1102 6 0.2756051221299245 1102 7 0.30749879598279506 1102 8 0.2551092978986489 1102 9 0.3114562459279995 1102 10-11 0.3068108766377904 1102 12-13 0.4709618548148171 1102 14-15 0.4679188861529582 1102 16-17 0.5715167585636323 1102 18-19 0.5270903724586162 1102 20-21 0.45149545082391285 1102 22-23 0.39530364638453364 1102 24-25 0.3914555184038946 1102 26-27 0.12940083294331828 1102 28-29 0.2744444991881423 1102 30-31 0.3607074102529708 1102 32-33 0.2790255535474344 1102 34-35 0.26795645716053684 1102 36-37 0.19965358351310414 1102 38-39 0.2805215963729282 1102 40-41 0.26467297671167955 1102 42-43 0.25504157550749795 1102 44-45 0.1930774716074879 1102 46-47 0.1957430195686669 1102 48-49 0.28484602689429295 1102 50-51 0.16401970210625194 1102 52-53 -0.029378406464374507 1102 54-55 0.16925907318259448 1102 56-57 -3.130190463606965 1102 58-59 -9.511801940203032 1102 60-61 -7.861676184262382 1102 62-63 -2.253995893628254 1102 64-65 -0.46335709639241074 1102 66-67 -1.6961053254672684 1102 68-69 -4.810269094795455 1102 70-71 -4.811110039598724 1102 72-73 -3.5273250112080667 1102 74-75 -4.056217082742986 1102 76-77 -4.172498699311475 1102 78-79 1.8200072262241989 1102 80-81 2.2303947682419647 1102 82-83 0.9033666688611497 1102 84-85 0.5858625288523527 1102 86-87 0.5542026163697749 1102 88-89 0.40675474249352206 1102 90-91 1.5898718234825502 1102 92-93 1.0279613282857838 1102 94-95 -8.966109200266061 1102 96-97 -9.59082380883135 1102 98-99 -8.71976636371636 1102 100-101 -9.708609728990654 1103 1 0.20249322319675045 1103 2 0.21094361662399663 1103 3 0.22360847799791372 1103 4 0.18415619131466343 1103 5 0.18571279794073092 1103 6 0.36155856452569424 1103 7 0.33177487681856377 1103 8 0.37575925200252414 1103 9 0.4449525879522582 1103 10-11 0.2938668912137672 1103 12-13 0.35613699373481467 1103 14-15 0.46039442486321036 1103 16-17 0.4023504596074332 1103 18-19 0.40095538539127773 1103 20-21 0.3508725577249976 1103 22-23 0.39595008005457544 1103 24-25 0.41036373653977165 1103 26-27 0.4823826430587914 1103 28-29 0.42269071332371766 1103 30-31 0.40533303661457865 1103 32-33 0.3755341726186501 1103 34-35 0.42717207148290015 1103 36-37 0.4694279961372345 1103 38-39 0.40258172122849345 1103 40-41 0.38257528813479524 1103 42-43 0.2201404152822235 1103 44-45 0.23375565841895707 1103 46-47 0.24061084219098916 1103 48-49 0.10347284999036077 1103 50-51 0.025410875297474433 1103 52-53 0.2473137118914437 1103 54-55 0.19994556576798317 1103 56-57 0.5331387590029593 1103 58-59 -8.366780571276212 1103 60-61 -7.1735557311010645 1103 62-63 -2.0918704623285116 1103 64-65 -0.30850221309532344 1103 66-67 -1.7037622690436578 1103 68-69 -3.438474134235232 1103 70-71 -3.832047433662824 1103 72-73 -2.9798871653727126 1103 74-75 -3.7283138366207247 1103 76-77 -0.398006319506198 1103 78-79 3.513985926268056 1103 80-81 2.9506441065628444 1103 82-83 1.5000547714724775 1103 84-85 1.104109800183263 1103 86-87 0.9562625228625805 1103 88-89 0.893676144414421 1103 90-91 2.298140369205182 1103 92-93 1.6718259071558847 1103 94-95 -6.996147794553105 1103 96-97 -8.785267994376856 1103 98-99 -7.863938248232284 1103 100-101 -9.190989451717877 1104 1 0.22788100499314723 1104 2 0.11396072509407063 1104 3 0.21146313814242745 1104 4 0.1703734900803866 1104 5 0.2953895803688553 1104 6 0.1794556836092127 1104 7 0.15697650884212777 1104 8 0.22332191819379688 1104 9 0.3454422675746116 1104 10-11 0.22477446463850015 1104 12-13 0.2130395382135717 1104 14-15 0.35910060102453656 1104 16-17 0.1859786855513903 1104 18-19 0.39816404570888864 1104 20-21 0.27954524558364824 1104 22-23 0.2560844633997732 1104 24-25 0.4113201380034681 1104 26-27 0.3189214284937023 1104 28-29 0.26316665455387067 1104 30-31 0.27900655105698746 1104 32-33 0.40491222922953085 1104 34-35 0.27472281828448786 1104 36-37 0.260386646737949 1104 38-39 0.038522749270676115 1104 40-41 0.15172728574677308 1104 42-43 0.0963479660601152 1104 44-45 0.22600339101418854 1104 46-47 0.07544295240492715 1104 48-49 0.005661042642941538 1104 50-51 0.03844265498155863 1104 52-53 -0.018317660771387523 1104 54-55 -0.2084307335102764 1104 56-57 0.13298968889673546 1104 58-59 -8.308690259615265 1104 60-61 -6.962557528855971 1104 62-63 -2.151698154594744 1104 64-65 -0.5643037169077161 1104 66-67 -1.7941533494015935 1104 68-69 -3.6844839889793306 1104 70-71 -4.073000228215559 1104 72-73 -2.980927391239966 1104 74-75 -4.001677654225041 1104 76-77 -0.4127437379425878 1104 78-79 3.3548378465076496 1104 80-81 2.8595404539954323 1104 82-83 1.3413049547978737 1104 84-85 0.7247325986590347 1104 86-87 0.7349443316455115 1104 88-89 0.6465036309416234 1104 90-91 2.1456098827748917 1104 92-93 1.857661500422779 1104 94-95 -5.950887280744492 1104 96-97 -7.4309487184953245 1104 98-99 -5.510381813290644 1104 100-101 -4.855606717523896 1105 1 0.23653143187164716 1105 2 0.17426215842914416 1105 3 0.19800680136063065 1105 4 0.20943541739106308 1105 5 0.3265051178602789 1105 6 0.2738594064095139 1105 7 0.18856114068875485 1105 8 0.1850768848192743 1105 9 0.29213901534529185 1105 10-11 0.2358772872733752 1105 12-13 0.2281270260247581 1105 14-15 0.3464121129006159 1105 16-17 0.4182749221964315 1105 18-19 0.43276535769644653 1105 20-21 0.365353783364597 1105 22-23 0.4631139438309688 1105 24-25 0.4396656720089922 1105 26-27 0.5323915295955359 1105 28-29 0.6119470092303487 1105 30-31 0.49262655667873645 1105 32-33 0.19949359613725903 1105 34-35 0.38031150137657477 1105 36-37 0.3796854627427422 1105 38-39 0.3993257892341333 1105 40-41 0.4729163753345702 1105 42-43 0.4434482562710542 1105 44-45 0.40512122994211097 1105 46-47 0.3787622893760414 1105 48-49 0.2807032716822775 1105 50-51 0.2615269490486085 1105 52-53 0.1935489761516891 1105 54-55 0.07708490375927823 1105 56-57 0.6331153344617988 1105 58-59 -8.331700010133009 1105 60-61 -7.2122569287632565 1105 62-63 -1.7481479356576735 1105 64-65 -0.04587983175171573 1105 66-67 -1.5582462200639178 1105 68-69 -2.8637752003291475 1105 70-71 -3.4837238464703173 1105 72-73 -2.628922441358437 1105 74-75 -3.3237465264525987 1105 76-77 0.20482900068773802 1105 78-79 4.080789839090734 1105 80-81 3.445932261429963 1105 82-83 1.8830637132034695 1105 84-85 1.5654178455756416 1105 86-87 1.4081880756038423 1105 88-89 1.2982034453606204 1105 90-91 2.4930164422908057 1105 92-93 2.2007710607186723 1105 94-95 -5.98420616417863 1105 96-97 -7.552784441072028 1105 98-99 -5.27833713146002 1105 100-101 -4.838185491887664 1106 1 0.07649533683318666 1106 2 0.07511188908481259 1106 3 0.08527339912151533 1106 4 0.06390093069084202 1106 5 0.1200880253623211 1106 6 0.15390538652987829 1106 7 0.21432514160320437 1106 8 0.18190931175177383 1106 9 0.18542118452509015 1106 10-11 0.1387438805264054 1106 12-13 0.15465623531481043 1106 14-15 0.23833669326761964 1106 16-17 0.22809133980412355 1106 18-19 0.10896452760716357 1106 20-21 0.07639263463435952 1106 22-23 0.32275302564531927 1106 24-25 0.18800541293612838 1106 26-27 0.034454275716363725 1106 28-29 0.20139210838705424 1106 30-31 0.40612565027790737 1106 32-33 0.18954814118199437 1106 34-35 0.21748818353094634 1106 36-37 0.2553552011905609 1106 38-39 0.46960002780914323 1106 40-41 0.23372476252066576 1106 42-43 0.23974956129506353 1106 44-45 0.26989604648851184 1106 46-47 0.42346491584552837 1106 48-49 0.16019100226711203 1106 50-51 -0.020092030952618245 1106 52-53 0.16815043347679648 1106 54-55 0.027913463596227928 1106 56-57 0.9291852094332214 1106 58-59 -8.00426713692287 1106 60-61 -7.527247317372552 1106 62-63 -2.0142723717733197 1106 64-65 -0.10886824978442533 1106 66-67 0.8384176452928891 1106 68-69 -2.070493353138989 1106 70-71 -2.3492819028120646 1106 72-73 -0.9537661153890085 1106 74-75 -0.9967004432955555 1106 76-77 2.348575237433842 1106 78-79 4.486542666964738 1106 80-81 3.3503858611131214 1106 82-83 1.790283606367307 1106 84-85 1.491277316417868 1106 86-87 1.3008033237451606 1106 88-89 1.2005560626533942 1106 90-91 2.704993893356434 1106 92-93 2.1864159576658864 1106 94-95 -5.653747393341877 1106 96-97 -7.353046218648025 1106 98-99 -5.1539400570578735 1106 100-101 -4.590357311998494 1107 1 0.12034701534549086 1107 2 0.10355282893552697 1107 3 0.0975287587019622 1107 4 0.03181916882342506 1107 5 0.14707056186389167 1107 6 0.1295160281028771 1107 7 0.11825308029632708 1107 8 0.12695675653782956 1107 9 0.05366491836959142 1107 10-11 0.13795866620542085 1107 12-13 0.13162518356327269 1107 14-15 0.14472296477543978 1107 16-17 0.23814405392802485 1107 18-19 0.19911895654785638 1107 20-21 0.13016892427368987 1107 22-23 0.07919966633870956 1107 24-25 0.061257092278246716 1107 26-27 0.27095367202451115 1107 28-29 0.08257683809491567 1107 30-31 0.0055652126324332585 1107 32-33 0.22815433908505156 1107 34-35 0.16346087425400668 1107 36-37 0.16996495900847464 1107 38-39 0.11418526453518041 1107 40-41 0.013678043824477015 1107 42-43 0.005894169351748246 1107 44-45 0.021255390435207744 1107 46-47 0.035991210671198814 1107 48-49 0.012902636716127347 1107 50-51 0.13504277281641208 1107 52-53 -0.04955205223998149 1107 54-55 -0.10168061068069534 1107 56-57 0.4013132128520738 1107 58-59 -8.423513497138124 1107 60-61 -7.475135329421494 1107 62-63 -2.1554143866519375 1107 64-65 -0.3858886466858884 1107 66-67 0.9326117270381609 1107 68-69 -2.4774065584315643 1107 70-71 -2.5083225513970184 1107 72-73 -0.8388530594634922 1107 74-75 -0.7101604896823233 1107 76-77 2.4182705286373682 1107 78-79 4.400140048062333 1107 80-81 3.106852728595502 1107 82-83 1.5270469882034412 1107 84-85 1.1715389784583046 1107 86-87 0.9857078118103182 1107 88-89 0.8955034107657696 1107 90-91 2.189360660016046 1107 92-93 2.048269063463408 1107 94-95 -6.430865293738943 1107 96-97 -7.820083294870862 1107 98-99 -5.616688016745366 1107 100-101 -4.75161720237967 1108 1 -0.058995902433100156 1108 2 -0.07525595852749589 1108 3 -0.11152770621158936 1108 4 -0.14753088428381034 1108 5 -0.08100919887834124 1108 6 -0.0896185788526438 1108 7 -0.09938157864515773 1108 8 -0.06457228323041875 1108 9 -0.18761978735926732 1108 10-11 -0.18443204604308505 1108 12-13 -0.19769291346496942 1108 14-15 -0.22330021281065626 1108 16-17 -0.17592365422481038 1108 18-19 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98-99 -0.5460698380320501 2312 100-101 0.14185909859320311 2313 1 -0.24669338553782794 2313 2 -0.23253780288877124 2313 3 -0.18721759435073082 2313 4 -0.16462111235054522 2313 5 -0.1820453662206134 2313 6 -0.32107577732244863 2313 7 -0.22146100638876476 2313 8 -0.24525811152653887 2313 9 -0.15126941512921377 2313 10-11 -0.2066707046667986 2313 12-13 -0.23053823603842005 2313 14-15 -0.314300842267798 2313 16-17 -0.3861498958471685 2313 18-19 -0.22035148280178163 2313 20-21 -0.3917829815767533 2313 22-23 -0.2414988152319708 2313 24-25 -0.3515085511390623 2313 26-27 -0.3211571551343937 2313 28-29 -0.13607172304367054 2313 30-31 -0.3276166965478069 2313 32-33 -0.4214854093210363 2313 34-35 -0.371636078060412 2313 36-37 -0.5162201022455335 2313 38-39 -0.45288210159360887 2313 40-41 -0.3442600545945851 2313 42-43 -0.21226258248015029 2313 44-45 -0.38963187849613234 2313 46-47 -0.4971193529637148 2313 48-49 -0.6711961929513564 2313 50-51 -0.7139911768362097 2313 52-53 -0.5400238214665052 2313 54-55 -0.5411574236338126 2313 56-57 0.18259391901465705 2313 58-59 3.145926606310365 2313 60-61 3.0575311758622625 2313 62-63 0.3124144923088359 2313 64-65 -0.3856504026136456 2313 66-67 -2.2828127508676985 2313 68-69 -4.973955721998248 2313 70-71 -3.7685555556317887 2313 72-73 -2.6363524925804924 2313 74-75 -3.25245021694521 2313 76-77 0.5110955773075894 2313 78-79 2.7979453426474272 2313 80-81 2.148759937508739 2313 82-83 0.6731947883909761 2313 84-85 0.2549517264823855 2313 86-87 0.4004710794260795 2313 88-89 0.5241452510212028 2313 90-91 2.255475560561276 2313 92-93 3.6487452451846494 2313 94-95 6.0162575452111255 2313 96-97 1.7465716766419916 2313 98-99 3.087711097487176 2313 100-101 4.257046148787497 2314 1 -0.35589331289079595 2314 2 -0.3767605269213945 2314 3 -0.33656445346903396 2314 4 -0.25370295678722243 2314 5 -0.342800013478346 2314 6 -0.4813354379600412 2314 7 -0.42436562776861564 2314 8 -0.5130962640244121 2314 9 -0.559959343542495 2314 10-11 -0.44230716927604163 2314 12-13 -0.510337165439573 2314 14-15 -0.7695203003335678 2314 16-17 -0.6724650117832169 2314 18-19 -0.5712822991045385 2314 20-21 -0.6946441932074094 2314 22-23 -0.6584625788519816 2314 24-25 -0.7269493411859997 2314 26-27 -0.7923067313775576 2314 28-29 -0.6716089278757522 2314 30-31 -0.7030577490478862 2314 32-33 -0.579049151314905 2314 34-35 -0.6145340961192858 2314 36-37 -0.5965724928261906 2314 38-39 -0.7664813209529981 2314 40-41 -0.9063380955603648 2314 42-43 -0.8078556268420769 2314 44-45 -0.8243356371400239 2314 46-47 -0.7591108368842612 2314 48-49 -0.6699122721954112 2314 50-51 -0.8151366798066491 2314 52-53 -0.8399061899696108 2314 54-55 -0.6563496810562484 2314 56-57 0.18162048032213107 2314 58-59 3.068626808189318 2314 60-61 3.113129511279471 2314 62-63 0.39054390480223233 2314 64-65 -0.3119315151210067 2314 66-67 -2.125769453448452 2314 68-69 -4.845226131666601 2314 70-71 -3.6059957556840683 2314 72-73 -2.892186038500867 2314 74-75 -4.286741819179628 2314 76-77 0.47239345007240985 2314 78-79 3.006620258741158 2314 80-81 2.3676206599267537 2314 82-83 0.7972818451469905 2314 84-85 0.40302280089204956 2314 86-87 0.39899215598785887 2314 88-89 0.6468111240295755 2314 90-91 2.1604599131051287 2314 92-93 3.6128818111008023 2314 94-95 6.171784650846416 2314 96-97 3.4391697632536236 2314 98-99 5.838356469755958 2314 100-101 6.248229298036199 2315 1 -0.3416417672777108 2315 2 -0.34763669447377765 2315 3 -0.4369020628990903 2315 4 -0.32246632524162777 2315 5 -0.41028228925457455 2315 6 -0.37150656502858936 2315 7 -0.41489118505207045 2315 8 -0.47953282958911814 2315 9 -0.49297789306994133 2315 10-11 -0.4985775780428199 2315 12-13 -0.5725732554644125 2315 14-15 -0.7655736558630934 2315 16-17 -0.617117575759174 2315 18-19 -0.5617763852421476 2315 20-21 -0.6340383627978241 2315 22-23 -0.6239959767915906 2315 24-25 -0.5211814940619774 2315 26-27 -0.6000359362753329 2315 28-29 -0.6305092392230449 2315 30-31 -0.4213684756081193 2315 32-33 -0.7279470100832555 2315 34-35 -0.6897948996753129 2315 36-37 -0.3620798999401913 2315 38-39 -0.6378826225561767 2315 40-41 -0.610212519177189 2315 42-43 -0.5569166472234386 2315 44-45 -0.630897967914791 2315 46-47 -0.5118821968392879 2315 48-49 -0.3925983019367223 2315 50-51 -0.4416300670677842 2315 52-53 -0.27278935930933557 2315 54-55 -0.39297552928502455 2315 56-57 0.2396806748572189 2315 58-59 3.496520987004029 2315 60-61 3.5037431335106852 2315 62-63 0.8145891082197814 2315 64-65 0.01146163871103667 2315 66-67 -1.5956526520889014 2315 68-69 -4.518804422653156 2315 70-71 -3.299845534138054 2315 72-73 -2.817359862321048 2315 74-75 -0.23680307939283196 2315 76-77 2.228845577576127 2315 78-79 3.691722686468758 2315 80-81 2.784528153206576 2315 82-83 0.9729453708486062 2315 84-85 0.8134727406085283 2315 86-87 0.8160713840549114 2315 88-89 0.8268822379670624 2315 90-91 2.4764633163297276 2315 92-93 4.005347076180094 2315 94-95 6.537072953672634 2315 96-97 4.43180958467115 2315 98-99 6.363849454403464 2315 100-101 6.362477425231718 2316 1 -0.5103116564955457 2316 2 -0.39491341572309935 2316 3 -0.36473810306389964 2316 4 -0.3518110545863564 2316 5 -0.5108643555900656 2316 6 -0.5500743696237933 2316 7 -0.3834434344262405 2316 8 -0.40775022355993684 2316 9 -0.7685355672577501 2316 10-11 -0.6230631128023276 2316 12-13 -0.6611362652603034 2316 14-15 -0.5917242683150761 2316 16-17 -0.8260897045943167 2316 18-19 -0.7936464684820947 2316 20-21 -0.589382612662618 2316 22-23 -0.8764073148193745 2316 24-25 -0.7186946100682405 2316 26-27 -0.7987237781138603 2316 28-29 -0.8208588692987036 2316 30-31 -0.7472904987904201 2316 32-33 -0.606437596108087 2316 34-35 -0.760709424014486 2316 36-37 -0.3855638234241141 2316 38-39 -0.7099375843371618 2316 40-41 -0.6593394196739482 2316 42-43 -0.5504160701749257 2316 44-45 -0.5000027342798319 2316 46-47 -0.6425346240670535 2316 48-49 -0.5122670147561266 2316 50-51 -0.41088453152772786 2316 52-53 -0.5848056281749194 2316 54-55 -0.4133966353225631 2316 56-57 0.3986756626193326 2316 58-59 3.526075628202502 2316 60-61 3.5135624364806795 2316 62-63 0.9653342534854765 2316 64-65 0.242224457830023 2316 66-67 -1.7236481472076832 2316 68-69 -2.7569322765344353 2316 70-71 -3.0192496966929028 2316 72-73 -0.40205804085484687 2316 74-75 5.745518328271043 2316 76-77 5.704918692690338 2316 78-79 4.764446968782082 2316 80-81 3.1296589229948815 2316 82-83 1.2906114186516398 2316 84-85 1.0411392901928842 2316 86-87 1.0164290426317493 2316 88-89 1.0598498284688986 2316 90-91 2.6183996904578812 2316 92-93 4.069312207953445 2316 94-95 6.4661628896118835 2316 96-97 4.090800112154827 2316 98-99 6.130468730611781 2316 100-101 6.192527436103644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 14.0 4 30.0 5 89.0 6 189.0 7 471.0 8 1048.0 9 1832.0 10 2993.0 11 4620.0 12 6929.0 13 9629.0 14 11890.0 15 15521.0 16 20808.0 17 27281.0 18 27891.0 19 26195.0 20 27060.0 21 27525.0 22 27929.0 23 28365.0 24 29753.0 25 31822.0 26 39102.0 27 54931.0 28 78079.0 29 103157.0 30 125255.0 31 139537.0 32 145399.0 33 146138.0 34 147728.0 35 157798.0 36 158498.0 37 123261.0 38 51364.0 39 3746.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.981082044716175 20.45974895462452 14.742109293514153 21.81705970714515 2 14.658297762162622 22.599091514425382 40.27109307125896 22.471517652153036 3 19.392597840542518 27.518808079699358 31.300381012251933 21.78821306750619 4 11.880883495086428 17.95001998468857 40.00463445205089 30.16446206817412 5 11.925763249846481 39.94782326030845 34.955031815829315 13.17138167401575 6 28.000682971881186 39.02596679049228 18.661874037145466 14.311476200481074 7 25.554789922395106 35.06506249580765 22.285838145642643 17.094309436154603 8 24.48585769104119 35.08164077615005 20.95701594010238 19.47548559270638 9 25.976325489319972 17.44266944438131 21.74494880759873 34.83605625869999 10-11 23.478904650583935 27.258616283446635 28.79971018043873 20.462768885530696 12-13 26.26819618367287 25.175607481868262 28.388873767172 20.167322567286867 14-15 22.760489080180676 26.20850878885245 28.435712876051344 22.595289254915528 16-17 20.19942002826129 29.521653590231285 29.026221796227137 21.252704585280295 18-19 19.993059389891545 29.078634008102554 31.076748069157745 19.851558532848156 20-21 21.67360263786802 27.392133960056125 30.278410826434783 20.655852575641077 22-23 20.774319370291057 27.576042987314576 30.058634688208368 21.591002954186003 24-25 21.16957401244762 27.62045015214435 30.549055673911607 20.660920161496417 26-27 21.454737263419553 26.99391145414964 31.216284462538784 20.335066819892024 28-29 20.371929781768227 27.84908158162875 31.290669685375043 20.488318951227978 30-31 20.718425007747562 27.934774112107096 30.782523271794492 20.56427760835085 32-33 20.6504263809565 27.885929927386066 30.49635599788445 20.967287693772988 34-35 21.31647382901784 27.340675411301056 30.52206698010754 20.820783779573567 36-37 21.495157277511083 27.0207324895189 31.001913119516704 20.482197113453314 38-39 21.40148651756366 27.215066795642812 31.07783203566577 20.305614651127758 40-41 20.995669434418705 27.531882783435655 31.118845034552976 20.353602747592667 42-43 20.42288239065546 28.211089412931223 30.32430841328661 21.04171978312671 44-45 20.82703308276601 27.997222248425096 29.80586812505098 21.36987654375791 46-47 21.127887069805688 28.11297791160815 29.152937540556234 21.60619747802993 48-49 21.328631949437654 27.85619890282549 29.406396190070396 21.408772957666464 50-51 21.271285265553345 28.273356789149524 29.687141131005284 20.768216814291847 52-53 21.1696179893951 28.660225399958172 29.247383582092034 20.92277302855469 54-55 20.43669529474048 27.95270862954918 29.798516376350875 21.812079699359465 56-57 21.209118928166472 28.57802989790688 28.82838104409686 21.384470129829786 58-59 20.683208483288336 27.75264334953638 30.223920393787186 21.340227773388094 60-61 20.68640389608403 27.80034516278416 30.38033267462219 21.132918266509616 62-63 21.835705138510836 27.74005865297728 29.268196005166786 21.156040203345107 64-65 21.325772789526333 28.204077027960096 29.360167221178614 21.109982961334953 66-67 21.113248549521963 27.804112737918473 29.451023980601626 21.63161473195794 68-69 20.846429607773324 27.76719665053376 30.28406635559288 21.102307386100033 70-71 21.76540896765856 27.7440183110061 29.026600517097457 21.463972204237884 72-73 20.989521836587393 27.40716758202287 29.61480520089051 21.98850538049923 74-75 20.830286754655226 28.25293173529752 29.395805886046166 21.52097562400109 76-77 19.866526262056773 27.946409448102095 30.03039028181171 22.156674008029423 78-79 20.964049937394286 27.75247719044736 29.389452135897326 21.894020736261027 80-81 20.772968828637225 28.65255916292212 28.833295668341023 21.74117634009963 82-83 20.930810769458482 28.393561874161183 28.605216868758937 22.0704104876214 84-85 20.903628133312896 28.073746238278268 28.47823959877186 22.544386029636975 86-87 21.01096067124999 28.272616726866325 28.614689012469658 22.101733589414025 88-89 21.376394216910217 27.697380091802117 28.52711932057565 22.399106370712023 90-91 20.932525860623358 27.719540333450965 28.94507122117316 22.402862584752516 92-93 20.64719325973007 28.495782894132937 28.820056191101884 22.036967655035113 94-95 19.78078134217835 28.446036030085892 29.04761958068261 22.725563047053146 96-97 19.408395806155916 28.349629447559472 28.80747847323149 23.43449627305312 98-99 20.21850682816403 28.00075876238045 28.485780876677403 23.294953532778116 100-101 19.77749664630849 28.07979276806951 29.098919124460704 23.043791461161298 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1079.0 1 781.5 2 477.0 3 520.0 4 524.5 5 534.0 6 718.0 7 1115.5 8 1538.0 9 1880.0 10 1998.5 11 1966.0 12 2089.5 13 2278.0 14 2432.0 15 2589.5 16 2851.0 17 3141.5 18 3449.0 19 3868.0 20 4377.5 21 4932.5 22 5657.0 23 6673.5 24 8236.0 25 10339.0 26 13003.0 27 16089.5 28 19186.5 29 22746.0 30 26791.5 31 31120.5 32 35902.5 33 41000.5 34 46056.5 35 50966.0 36 55581.5 37 59117.0 38 61136.0 39 62267.5 40 62844.0 41 63369.5 42 64148.0 43 65549.0 44 67988.5 45 70874.0 46 75076.5 47 82288.0 48 91577.0 49 86286.0 50 69990.0 51 59104.0 52 50867.5 53 44653.0 54 39533.0 55 34603.5 56 30490.0 57 26712.0 58 22937.0 59 19260.5 60 15740.0 61 12447.5 62 9339.5 63 6772.0 64 4697.5 65 3255.5 66 2169.0 67 1423.0 68 965.5 69 665.5 70 518.5 71 394.5 72 274.5 73 204.0 74 154.5 75 108.0 76 60.0 77 30.5 78 17.0 79 9.0 80 4.5 81 3.0 82 1.5 83 0.5 84 0.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025611445544356858 2 0.012195926449693745 3 1.1087205863357948E-4 4 0.0 5 0.06441666606610968 6 0.0 7 0.0 8 1.6630808795036923E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.7718014658394873E-4 16-17 0.0 18-19 2.7718014658394873E-4 20-21 0.0 22-23 0.0 24-25 1.1087205863357948E-4 26-27 1.1087205863357948E-4 28-29 0.001053284557019005 30-31 0.0048783705798774975 32-33 0.005266422785095025 34-35 0.0017739529381372717 36-37 0.0880878505843789 38-39 0.08207304140350721 40-41 0.08736718220326065 42-43 0.09038844580102567 44-45 0.09446299395580972 46-47 0.0886699288922052 48-49 0.0794398300109597 50-51 0.07445058737244863 52-53 0.06264271312797241 54-55 0.05158322527927285 56-57 0.06724390356126596 58-59 0.7243271590531748 60-61 0.3425115071337854 62-63 0.002827237495156277 64-65 0.002356031245963564 66-67 1.098409484882872 68-69 2.3773186812212117 70-71 2.34591417061325 72-73 1.8033617516898286 74-75 1.285838700002938 76-77 0.7395997851299504 78-79 0.5607354365393282 80-81 0.038999246624361585 82-83 1.1087205863357948E-4 84-85 0.0010810025716774 86-87 0.0012473106596277692 88-89 5.543602931678974E-5 90-91 0.3284861917166376 92-93 0.0513614811620057 94-95 0.9569921740957413 96-97 1.6654646287643144 98-99 0.6368768228059389 100-101 0.5729313629890219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1803881.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.79183168364423 #Duplication Level Percentage of deduplicated Percentage of total 1 74.71579849872188 39.44383858453604 2 14.139183492222651 14.928667901311584 3 4.943446148583783 7.829207310395833 4 2.2534618058627354 4.75857505442506 5 1.1739265931407092 3.0986867557019115 6 0.702058834070464 2.223778308015807 7 0.4270621089996565 1.5781773683740397 8 0.3090495145794361 1.3052231964471634 9 0.20964506395714866 0.9960792236759358 >10 1.000019347368009 9.394470000950498 >50 0.07586716069092768 2.728722904488322 >100 0.044382225395149286 4.231621324698037 >500 0.0019980158920819145 0.7678865279615326 >1k 0.0033650793971905925 3.656454473514921 >5k 7.361111181354421E-4 3.0586110655032273 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9777 0.5419980586302533 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9704 0.5379512284901277 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8967 0.4970948748836536 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8332 0.46189299626749214 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7553 0.4187083294297129 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5471 0.3032905163921567 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5291 0.2933120311151345 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4679 0.2593851811732592 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3824 0.21198737610740398 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3737 0.20716444155684327 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3593 0.19918165333522556 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3315 0.183770437185158 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3268 0.18116494380726889 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3054 0.16930163353347588 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2505 0.13886725343855832 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2494 0.13825745711607362 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2389 0.1324366740378107 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2204 0.1221810086142046 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2148 0.11907659097246438 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1932 0.10710240864003778 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.543602931678974E-5 0.0 16-17 0.0 0.0 0.0 5.543602931678974E-5 0.0 18-19 2.771801465839487E-5 0.0 0.0 1.3859007329197436E-4 0.0 20-21 5.543602931678974E-5 0.0 0.0 2.7718014658394873E-4 0.0 22-23 5.543602931678974E-5 0.0 0.0 7.483863957766615E-4 0.0 24-25 1.1087205863357948E-4 0.0 0.0 0.0011364386009941898 0.0 26-27 1.6630808795036923E-4 0.0 0.0 0.0020234150700628255 0.0 28-29 2.7718014658394873E-4 0.0 0.0 0.004462600360001574 0.0 30-31 3.880522052175282E-4 0.0 0.0 0.01302746688944559 0.0 32-33 3.880522052175282E-4 0.0 0.0 0.031792562813178916 0.0 34-35 4.989242638511077E-4 0.0 0.0 0.06225466092275488 0.0 36-37 7.761044104350564E-4 0.0 0.0 0.10735187077196334 0.0 38-39 8.59258454410241E-4 0.0 0.0 0.16672385817024515 0.0 40-41 8.869764690686359E-4 0.0 0.0 0.23385688967287754 0.0 42-43 9.146944837270307E-4 0.0 0.0 0.3062286259459466 0.0 44-45 9.424124983854256E-4 0.0 0.0 0.3896044140383983 0.0 46-47 9.701305130438206E-4 0.0 0.0 0.48997134511644613 0.0 48-49 0.0010532845570190052 0.0 0.0 0.5897561978866677 0.0 50-51 0.0010532845570190052 0.0 0.0 0.6910378234484426 0.0 52-53 0.0010810025716774 0.0 0.0 0.7818420394693442 0.0 54-55 0.0011641566156525846 0.0 0.0 0.8884455238455309 0.0 56-57 0.0012195926449693744 0.0 0.0 0.9939680056500401 0.0 58-59 0.0012195926449693744 0.0 0.0 1.074128504042118 0.0 60-61 0.0012750286742861641 0.0 0.0 1.1436730028200308 0.0 62-63 0.0013859007329197436 0.0 0.0 1.2010215751482498 0.0 64-65 0.001496772791553323 0.0 0.0 1.2660757555515025 0.0 66-67 0.0015522088208701128 0.0 5.543602931678974E-5 1.3210128606044411 0.0 68-69 0.0015522088208701128 0.0 5.543602931678974E-5 1.3882013281363905 0.0 70-71 0.0015522088208701128 0.0 5.543602931678974E-5 1.4940564261168006 0.0 72-73 0.0016076448501869025 0.0 5.543602931678974E-5 1.6437059872574742 0.0 74-75 0.0016630808795036923 0.0 5.543602931678974E-5 1.8357641108254923 0.0 76-77 0.0016630808795036923 0.0 5.543602931678974E-5 2.0723928019642095 0.0 78-79 0.0016630808795036923 0.0 5.543602931678974E-5 2.3787322999687897 0.0 80-81 0.0017739529381372717 0.0 5.543602931678974E-5 2.7492390019075534 0.0 82-83 0.0017739529381372717 0.0 5.543602931678974E-5 3.1051937461506607 0.0 84-85 0.0018016709527956666 2.771801465839487E-5 5.543602931678974E-5 3.4466242507127687 0.0 86-87 0.0018293889674540615 5.543602931678974E-5 5.543602931678974E-5 3.7578421192972264 0.0 88-89 0.0018293889674540615 5.543602931678974E-5 5.543602931678974E-5 4.050017711811367 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9695 0.0 25.79963 1 TACATGG 9720 0.0 25.19499 2 ACATGGG 9550 0.0 24.354788 3 CATGGGA 7180 0.0 22.9977 4 GCTTACG 245 0.0 22.431353 60-61 ATGGGAT 2710 0.0 21.935223 5 GAGTACT 7485 0.0 21.164074 12-13 AGTACTT 7895 0.0 20.572588 12-13 CATTCCG 330 3.092282E-11 20.001654 9 ATGGGAG 2720 0.0 18.905947 5 GTACTTT 8385 0.0 18.808098 14-15 CATGGGG 2525 0.0 18.497568 4 CGAATCG 80 0.0010621906 18.120527 72-73 TAGGACA 890 0.0 16.962927 4 TACTTTT 9560 0.0 16.866308 14-15 ACTTTTT 9600 0.0 16.624142 16-17 GTGTAGC 1030 0.0 16.494938 1 TAGCCCT 1230 0.0 16.493172 4 GGTATCG 235 1.0913936E-11 16.485905 62-63 GTATAGC 900 0.0 16.25566 1 >>END_MODULE