FastQCFastQC Report
Fri 27 May 2016
SRR939622_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939622_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311263
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28070.9018097236099375No Hit
NTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27520.8841397789008009No Hit
NTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24890.7996453160189293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24570.7893646209154316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22680.7286442654603984No Hit
NGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19850.6377243681388408No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18470.593388870505007No Hit
NTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18070.580538001625635No Hit
NATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.5718636651320588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16230.5214240047805232No Hit
NAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15780.5069667772912295No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15520.49861371251963776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.4693779858190662No Hit
NAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13800.44335497633833765No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13360.42921902057102834No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11530.37042629544790096No Hit
NCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9260.29749761455746426No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8320.2672980726909398No Hit
NTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.24416650870807002No Hit
NCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT7340.23581344393647816No Hit
NCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6430.20657771723590662No Hit
NCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6390.2052926303479694No Hit
NTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA6220.19983101107423623No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6120.19661829385439322No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6050.1943693918005031No Hit
NTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG5670.1821610663650996No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG5670.1821610663650996No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.1686676540417589No Hit
NGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC5240.16834638231977458No Hit
NTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5170.16609748026588447No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA4950.15902950238222982No Hit
NCGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4940.1587082306602455No Hit
NCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT4930.1583869589382612No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.1564593286063554No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC4650.14939135072270074No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4630.14874880727873213No Hit
NTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4630.14874880727873213No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT4560.14649990522484202No Hit
NTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG4260.13686175356531294No Hit
NATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4250.13654048184332865No Hit
NAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.13589793839936004No Hit
NATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG4000.12850868879372107No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3980.12786614534975246No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG3930.12625978673983096No Hit
NGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3890.12497469985189374No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.12465342812990944No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3840.12336834124197223No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3720.1195130805781606No Hit
NGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3640.11694290680228617No Hit
NCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3600.11565781991434895No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3590.11533654819236465No Hit
NTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3530.11340891786045884No Hit
NGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT3340.10730475514275709No Hit
NCGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA3280.10537712481085126No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3280.10537712481085126No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG3220.10344949447894546No Hit
NTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.10248567931299254No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG3130.10055804898108672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG255.4457654E-575.496469
CGAATGC250.005138489456.658833
CAGGCCT807.4578566E-1153.1090934
TCAGGCC654.618778E-850.8476643
CCCTATA502.9241162E-547.215692
GTCAGGC804.436515E-947.215692
GTGTTAT1103.8223352E-545.102941
TGTCAGG907.7465293E-444.1006551
GCTTTGC1000.001300953439.6905861
TTCGCGA250.001653082137.8212948-49
ACTGGGC500.001667575737.748238
AGGAGCC757.2362964E-637.7421537
TGGGAGT1003.2052412E-837.7421536
TACCTGG801.120229E-535.4117662
GGGAACA550.002664220534.3110477
GTATATG1250.003888063131.7524721
CGTACAA300.004040157431.52028774-75
GTTATGG600.00406424231.4771273
ACCTGGG902.5081601E-531.4771253
CGCAGAA1051.9967101E-631.4568588
Produced by FastQC (version 0.11.5)