FastQCFastQC Report
Fri 27 May 2016
SRR939621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044702
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57890.19013355001573226No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41710.13699206030672295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.1280913534395156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG91250.027.354841
TACATGG91000.026.805092
GTATCAA121950.025.760691
ACATGGG92550.025.0742533
ATCAACG140400.022.2410033
CAACGCA140650.022.2011055
TCAACGC140400.022.1730374
AACGCAG143950.021.8204336
TATCAAC144050.021.7762852
CATGGGA58700.020.9387194
AGTACAT63100.019.930222
GAGTACA71200.019.3952031
ACGCAGA158300.019.3931147
GGTATCA90500.019.1917151
CGCAGAG159150.018.9913988
AGAGTAC102850.018.8461510-11
GAGTACT62400.018.40217812-13
CATGGGG46000.017.5379774
GCAGAGT163800.017.2359189
GTACTTT67900.016.70302614-15
Produced by FastQC (version 0.11.5)