##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939621_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3044702 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.05498764739538 31.0 30.0 33.0 26.0 34.0 2 30.212743316094645 31.0 30.0 34.0 26.0 34.0 3 30.257502376258827 31.0 30.0 34.0 26.0 34.0 4 33.826074932784884 35.0 35.0 37.0 28.0 37.0 5 33.81125936134308 35.0 35.0 37.0 28.0 37.0 6 33.89854770680349 35.0 35.0 37.0 28.0 37.0 7 33.70571668426007 35.0 33.0 37.0 28.0 37.0 8 33.690073774050795 35.0 33.0 37.0 28.0 37.0 9 34.95349495615663 37.0 34.0 39.0 28.0 39.0 10-11 34.84317217251475 37.0 34.0 39.0 27.0 39.0 12-13 34.67143336195135 37.0 34.0 39.0 27.0 39.0 14-15 35.3649953263078 38.0 34.0 40.0 27.0 41.0 16-17 35.08789907846482 38.0 33.0 40.0 26.5 41.0 18-19 35.021144269619825 38.0 33.0 40.0 26.0 41.0 20-21 35.137983126099044 38.0 34.0 40.0 26.0 41.0 22-23 34.99190068519021 38.0 34.0 40.0 25.0 41.0 24-25 34.861630957643804 38.0 33.0 40.0 25.0 41.0 26-27 35.01513777046161 38.0 34.0 40.0 25.0 41.0 28-29 34.90169432016664 38.0 34.0 40.0 25.0 41.0 30-31 34.704308007811605 38.0 33.0 40.0 24.0 41.0 32-33 34.499243439916285 38.0 33.0 40.0 24.0 41.0 34-35 34.27829357355827 38.0 33.0 40.0 24.0 41.0 36-37 34.00310851439649 38.0 33.0 40.0 23.0 41.0 38-39 33.85526957974869 38.0 32.5 40.0 22.0 41.0 40-41 33.60001668471989 38.0 32.0 40.0 20.0 41.0 42-43 33.3013467327837 37.5 31.0 40.0 18.5 41.0 44-45 33.073890810989056 37.0 31.0 40.0 17.5 41.0 46-47 32.67178659849141 37.0 31.0 40.0 15.0 41.0 48-49 32.35223824860364 37.0 30.0 40.0 14.0 41.0 50-51 31.753890364311516 36.0 30.0 38.5 12.0 40.5 52-53 31.82374646188691 36.5 30.0 39.0 9.0 40.0 54-55 31.856270662941725 36.0 30.0 39.0 9.0 40.0 56-57 31.705976972459048 36.0 30.0 39.0 8.0 40.0 58-59 31.294796173812742 36.0 29.0 39.0 7.5 40.0 60-61 30.85661601693696 35.0 28.5 39.0 4.0 40.0 62-63 30.43609489532966 35.0 28.0 38.5 2.0 40.0 64-65 29.80629795625319 34.5 27.5 38.0 2.0 40.0 66-67 29.25277202826418 34.0 26.5 38.0 2.0 40.0 68-69 28.718786107803 34.0 26.0 37.0 2.0 39.0 70-71 28.144425135858945 33.5 26.0 36.5 2.0 39.0 72-73 27.383256226717755 32.5 24.5 36.0 2.0 38.5 74-75 26.904953095573887 32.0 24.0 35.0 2.0 38.0 76-77 26.50782326152116 32.0 22.5 35.0 2.0 37.5 78-79 25.90997312709093 31.5 21.5 35.0 2.0 37.0 80-81 25.21648325517571 31.0 19.0 34.5 2.0 36.0 82-83 24.53386521899352 31.0 17.5 34.0 2.0 36.0 84-85 23.851880085473063 30.0 10.0 34.0 2.0 35.0 86-87 23.166025115101576 30.0 4.5 34.0 2.0 35.0 88-89 22.351521265463745 29.0 2.0 34.0 2.0 35.0 90-91 21.08101991590638 28.0 2.0 33.5 2.0 35.0 92-93 19.409478004743978 25.0 2.0 32.0 2.0 34.0 94-95 19.12437456933388 25.0 2.0 32.0 2.0 34.0 96-97 18.479840391604824 25.0 2.0 32.0 2.0 34.0 98-99 17.477320276335746 22.5 2.0 32.0 2.0 34.0 100-101 15.877470438814703 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.816864646915846 1101 2 0.837820309692642 1101 3 0.8600581586705083 1101 4 1.0209313399616704 1101 5 0.7550310589652156 1101 6 0.7136243408720802 1101 7 0.6479828517939481 1101 8 0.7188672266430274 1101 9 0.9676704619303891 1101 10-11 0.8437931698630123 1101 12-13 0.6936972328903082 1101 14-15 0.8903422365185918 1101 16-17 0.7057539702483098 1101 18-19 0.7548555218292563 1101 20-21 0.9850954452069374 1101 22-23 1.0315167441012463 1101 24-25 0.7132155749853695 1101 26-27 0.8156224602872015 1101 28-29 0.8130893203742957 1101 30-31 0.8241077602916249 1101 32-33 0.6364794088247194 1101 34-35 0.8417556001167341 1101 36-37 0.6711384443169592 1101 38-39 0.5886219773919308 1101 40-41 0.7455814548889776 1101 42-43 0.720872400306682 1101 44-45 0.7018014643333714 1101 46-47 0.6579093227848603 1101 48-49 0.37692742568960824 1101 50-51 0.6065130461004102 1101 52-53 0.5782604683349994 1101 54-55 0.6386356311131571 1101 56-57 0.6398833590309394 1101 58-59 0.6019628813638498 1101 60-61 0.47805594151114406 1101 62-63 0.5500262405379424 1101 64-65 0.4538751208689611 1101 66-67 0.3348957009055553 1101 68-69 0.11079356274257535 1101 70-71 0.07089582206697287 1101 72-73 0.3275204034091068 1101 74-75 0.03815361726068289 1101 76-77 -0.3873546973609372 1101 78-79 -0.2342457578810091 1101 80-81 -0.5692389781176495 1101 82-83 -0.5463747528760443 1101 84-85 -0.7264566891574304 1101 86-87 -1.3090541977796484 1101 88-89 -1.6837629944146997 1101 90-91 -4.939116666019029 1101 92-93 -9.736672654206938 1101 94-95 -8.065932367818135 1101 96-97 -5.0329345202283875 1101 98-99 -3.9276892623695794 1101 100-101 -3.4228886809087733 1102 1 1.072114978845736 1102 2 1.0096061727199839 1102 3 0.9763672908013845 1102 4 1.1792495447359386 1102 5 1.0146971974959698 1102 6 0.9748376673518422 1102 7 1.073869340434534 1102 8 1.010485395288434 1102 9 1.186824404885293 1102 10-11 0.8663878894023114 1102 12-13 1.0019528378369458 1102 14-15 1.243612090867309 1102 16-17 1.2655249017247598 1102 18-19 1.1512604756542615 1102 20-21 1.2022008882154296 1102 22-23 1.2900513370034403 1102 24-25 1.2487901169092908 1102 26-27 1.048424513279194 1102 28-29 1.131397668322812 1102 30-31 1.2810713574169768 1102 32-33 1.2572215456639029 1102 34-35 1.137176668183379 1102 36-37 1.2380580130038297 1102 38-39 1.142638665973351 1102 40-41 1.1720201439568214 1102 42-43 0.9302544812905111 1102 44-45 0.893872174223759 1102 46-47 1.0273501468565627 1102 48-49 1.0869170234014902 1102 50-51 0.9892087981037037 1102 52-53 1.152158489975097 1102 54-55 1.0696830921142073 1102 56-57 1.0648360802784964 1102 58-59 0.9793083534807785 1102 60-61 0.9327708219169146 1102 62-63 0.9016380818199465 1102 64-65 0.6695292489602096 1102 66-67 0.6699244596064808 1102 68-69 0.5291957302174879 1102 70-71 0.48754898051865325 1102 72-73 0.5567287578390001 1102 74-75 0.3747151252010106 1102 76-77 0.23590818148673875 1102 78-79 0.16382202191390505 1102 80-81 -0.18716916021865515 1102 82-83 -0.3368742744734661 1102 84-85 -0.5664781611229088 1102 86-87 -0.9277260161779033 1102 88-89 -1.555645885570577 1102 90-91 -5.498931491172671 1102 92-93 -10.567804693960236 1102 94-95 -9.390426708164831 1102 96-97 -6.018632946280697 1102 98-99 -4.5622878116963825 1102 100-101 -3.789942304992797 1103 1 0.8044185811795685 1103 2 0.8259195213010493 1103 3 0.8378464870393643 1103 4 1.0059997352286913 1103 5 0.8945848405369645 1103 6 0.8241314571529799 1103 7 0.9059373281141632 1103 8 0.843910260727931 1103 9 0.9169832884138529 1103 10-11 0.9352975913692063 1103 12-13 1.0036111170042616 1103 14-15 1.0466277776625148 1103 16-17 1.091730107356156 1103 18-19 1.0401393559246586 1103 20-21 1.1325087477791982 1103 22-23 1.0679659280938836 1103 24-25 0.8927652887475688 1103 26-27 0.9471290170951789 1103 28-29 1.0737337401388416 1103 30-31 1.1264147212561681 1103 32-33 1.1858412041005764 1103 34-35 1.0594631568185378 1103 36-37 1.1387537297376227 1103 38-39 0.8896422102710417 1103 40-41 0.8173414922985103 1103 42-43 0.8978645831934884 1103 44-45 0.8624287658964391 1103 46-47 0.9464739466794825 1103 48-49 0.8724455584576702 1103 50-51 0.7597445092354391 1103 52-53 0.93021713244487 1103 54-55 0.8998510353472575 1103 56-57 0.7243202298475637 1103 58-59 0.7795145968223629 1103 60-61 0.6116921934481212 1103 62-63 0.829704132052683 1103 64-65 0.6337692862462951 1103 66-67 0.5383461219417356 1103 68-69 0.5768521654440271 1103 70-71 0.6046184404002659 1103 72-73 0.4605650336022755 1103 74-75 0.4859765041171258 1103 76-77 0.1320841238570445 1103 78-79 0.047869181000447014 1103 80-81 0.01807148402606984 1103 82-83 0.02832573824336393 1103 84-85 -0.21276636279026917 1103 86-87 -0.4743064556525276 1103 88-89 -1.1427267885238948 1103 90-91 -2.7714747787939764 1103 92-93 -9.078744183238097 1103 94-95 -5.826906387517784 1103 96-97 -3.3696027814127767 1103 98-99 -2.4415339507765594 1103 100-101 -1.903180143638215 1104 1 0.9537421712614318 1104 2 0.853106510133042 1104 3 0.8449756721827484 1104 4 0.9425014681954806 1104 5 0.9299112445450959 1104 6 0.83525275720217 1104 7 0.9316380453222024 1104 8 0.949776991959844 1104 9 1.0601253916902849 1104 10-11 0.8862098296607215 1104 12-13 0.8651875829721476 1104 14-15 0.9269106573233259 1104 16-17 1.0687645798593621 1104 18-19 0.9893694344324402 1104 20-21 0.9902627732570579 1104 22-23 0.9287050794167726 1104 24-25 0.708476281743259 1104 26-27 0.8202210378221437 1104 28-29 0.8161341374438749 1104 30-31 0.8348492555284892 1104 32-33 0.958894714454523 1104 34-35 0.7784997683632611 1104 36-37 0.765918127088419 1104 38-39 0.6266352364619152 1104 40-41 0.5196665084080436 1104 42-43 0.6615177496231155 1104 44-45 0.6955251905324005 1104 46-47 0.4180891327303442 1104 48-49 0.5389845244732143 1104 50-51 0.6430702803008046 1104 52-53 0.6810303304453846 1104 54-55 0.7296439563018602 1104 56-57 0.8246265970229985 1104 58-59 0.7864461779972736 1104 60-61 0.6822409090699892 1104 62-63 0.49648950726191643 1104 64-65 0.6238408545335723 1104 66-67 0.5815123418626555 1104 68-69 0.49308104669863084 1104 70-71 0.4009144860331517 1104 72-73 0.4738082170756073 1104 74-75 0.3758056667643537 1104 76-77 0.2527375038095343 1104 78-79 0.04161296119391622 1104 80-81 -0.01999878405950639 1104 82-83 -0.039743157195250234 1104 84-85 -0.16199005467678163 1104 86-87 -0.4918648026805563 1104 88-89 -0.5633718754010069 1104 90-91 -1.1246414943929643 1104 92-93 -4.347138605715953 1104 94-95 -1.87461111209236 1104 96-97 -1.3738554132726755 1104 98-99 -1.0420537454895822 1104 100-101 -0.7925874079909523 1105 1 1.0059043086450146 1105 2 1.0280352665602415 1105 3 0.905261486880697 1105 4 0.8923635650867396 1105 5 0.7978420867090676 1105 6 0.8036611032052718 1105 7 0.9637213372718705 1105 8 0.9672368243368936 1105 9 1.1847451076328 1105 10-11 1.0041032355237007 1105 12-13 0.8631895159076848 1105 14-15 1.040057675025345 1105 16-17 1.1698909159151114 1105 18-19 0.9486969944260224 1105 20-21 0.8213417791322684 1105 22-23 0.8899722600049031 1105 24-25 1.089339837886243 1105 26-27 1.0656230578543884 1105 28-29 0.9396061251586545 1105 30-31 1.1507877606740777 1105 32-33 1.1350871370416016 1105 34-35 1.178033724616263 1105 36-37 1.2077558674638524 1105 38-39 1.2948816131627794 1105 40-41 0.9533013058318218 1105 42-43 1.1405033786675745 1105 44-45 0.9803617696184972 1105 46-47 1.1095914744091644 1105 48-49 0.9914005028976831 1105 50-51 0.9695318240516144 1105 52-53 0.9290540375559893 1105 54-55 1.135457470042514 1105 56-57 0.9775225623065609 1105 58-59 0.707192191795226 1105 60-61 0.6853450660274092 1105 62-63 0.7197494832721247 1105 64-65 0.7565395534311143 1105 66-67 0.5775641491191905 1105 68-69 0.5874638825366283 1105 70-71 0.6135614617148377 1105 72-73 0.8174333498216733 1105 74-75 0.5878114502908538 1105 76-77 0.31299810630477864 1105 78-79 0.09391949573845082 1105 80-81 0.19509110126316997 1105 82-83 0.10430886398299677 1105 84-85 -0.12343754071456914 1105 86-87 -0.15916056473848528 1105 88-89 -0.2514795467866833 1105 90-91 -1.378603248245465 1105 92-93 -7.600183170194251 1105 94-95 -3.3525905450470006 1105 96-97 -1.6531882831089462 1105 98-99 -1.3326478748663817 1105 100-101 -1.2004818834625368 1106 1 0.8955486241892991 1106 2 0.9776759412725973 1106 3 0.8606422641155689 1106 4 0.9340246119086686 1106 5 0.7602854572919711 1106 6 0.7325388995089952 1106 7 0.8344680801753483 1106 8 0.9503324003123694 1106 9 0.9877806512545177 1106 10-11 0.9014534959673028 1106 12-13 0.9389870856523714 1106 14-15 0.7729035644019788 1106 16-17 0.9398986319964138 1106 18-19 1.02633670100807 1106 20-21 0.9337043687567217 1106 22-23 0.9634333879180872 1106 24-25 0.9749729289111855 1106 26-27 0.8530201940090834 1106 28-29 0.957115630626113 1106 30-31 0.7091129572996806 1106 32-33 0.8680344939217477 1106 34-35 0.8104887417240363 1106 36-37 0.9887566204540903 1106 38-39 0.9383589386407394 1106 40-41 0.890719542262481 1106 42-43 0.811707811380451 1106 44-45 0.6445753912946799 1106 46-47 0.7782108130189442 1106 48-49 0.8404036182913686 1106 50-51 0.7188289986832928 1106 52-53 0.721354505485639 1106 54-55 0.8039881668067217 1106 56-57 0.8371920740627274 1106 58-59 0.6550788543521655 1106 60-61 0.7054457681287509 1106 62-63 0.6850052930883059 1106 64-65 0.6950829310474447 1106 66-67 0.4871505009642192 1106 68-69 0.43846045684476564 1106 70-71 0.4800224696460589 1106 72-73 0.602774036532221 1106 74-75 0.5982976668458804 1106 76-77 0.4733304149645825 1106 78-79 0.4050691297934712 1106 80-81 0.4576671760326221 1106 82-83 0.6331442528693607 1106 84-85 0.6069030011901653 1106 86-87 0.26537843560491226 1106 88-89 0.1630161359313611 1106 90-91 -0.2612367902370849 1106 92-93 -3.9949292014805824 1106 94-95 -1.8335776449175007 1106 96-97 -0.793874062764143 1106 98-99 -0.27351890253256883 1106 100-101 -0.11841287728553418 1107 1 0.597747397063447 1107 2 0.7515720037956086 1107 3 0.572758373555665 1107 4 0.6043809997069118 1107 5 0.6017026097476688 1107 6 0.6694767943634261 1107 7 0.5512057706467104 1107 8 0.6288183934527112 1107 9 0.6206106119914594 1107 10-11 0.5313448295461214 1107 12-13 0.665804708933841 1107 14-15 0.6960352590851144 1107 16-17 0.7455447141716434 1107 18-19 0.6863993605530396 1107 20-21 0.6918274578371211 1107 22-23 0.7922138540273167 1107 24-25 0.6195390906882423 1107 26-27 0.4796952869951099 1107 28-29 0.5814180803267774 1107 30-31 0.7080729412469253 1107 32-33 0.5203611096443481 1107 34-35 0.5270374606063086 1107 36-37 0.6886089185223838 1107 38-39 0.559669907060659 1107 40-41 0.6808853656076579 1107 42-43 0.4887287833998286 1107 44-45 0.513985679954736 1107 46-47 0.31460392770929246 1107 48-49 0.31938929492756785 1107 50-51 0.5289963321462565 1107 52-53 0.7841287573567364 1107 54-55 0.6712607079005402 1107 56-57 0.37026851498402635 1107 58-59 0.4657538356308386 1107 60-61 0.40748080699876965 1107 62-63 0.3153285617279131 1107 64-65 0.3332830154485471 1107 66-67 0.3007895538514411 1107 68-69 0.35536550146564494 1107 70-71 0.22065984278582107 1107 72-73 0.37814318792877444 1107 74-75 0.39525229711525967 1107 76-77 0.16962948862109428 1107 78-79 0.2130041781793004 1107 80-81 0.1350039281623019 1107 82-83 0.1327957880178694 1107 84-85 0.021487739484683743 1107 86-87 -0.22967737669365462 1107 88-89 -0.2889951134118398 1107 90-91 -0.5621337588307718 1107 92-93 -3.7757747019501657 1107 94-95 -1.6449317654704139 1107 96-97 -0.726818420095249 1107 98-99 -0.41203766316028023 1107 100-101 -0.2982402894401215 1108 1 0.4503096521877268 1108 2 0.4668593777268022 1108 3 0.19597475305823053 1108 4 -0.10392926493709354 1108 5 -0.7317504279626235 1108 6 -0.04332939982614903 1108 7 0.13230011387543783 1108 8 0.2073773297194208 1108 9 0.5035969922372772 1108 10-11 0.4701511177800981 1108 12-13 0.2905709251480033 1108 14-15 0.32331318155308963 1108 16-17 0.40824116460190396 1108 18-19 0.4208767239118245 1108 20-21 0.44269490375779696 1108 22-23 0.34594165309992064 1108 24-25 0.25775555361730085 1108 26-27 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76-77 0.9529065734309761 2311 78-79 0.9259717265634642 2311 80-81 0.9043776225593625 2311 82-83 1.0517564591396997 2311 84-85 0.9553167268324003 2311 86-87 1.0249541367884767 2311 88-89 1.1881588379956938 2311 90-91 1.8411316365708252 2311 92-93 2.713939401281806 2311 94-95 2.0818653925538406 2311 96-97 1.7769512929720719 2311 98-99 1.5407113413582323 2311 100-101 1.7828718886353645 2312 1 0.15045217950243384 2312 2 -0.08372436616033951 2312 3 -0.21762024394215018 2312 4 -0.19967598808141673 2312 5 6.236335204619081E-4 2312 6 -0.03243060512728846 2312 7 0.09243009733209107 2312 8 -0.678104154455319 2312 9 -0.10275438122427971 2312 10-11 -0.08204033058101601 2312 12-13 -0.04735842929779466 2312 14-15 -0.44657528718725104 2312 16-17 0.02437029362184262 2312 18-19 0.08273130605591206 2312 20-21 0.18916626194857145 2312 22-23 -0.0880804713569674 2312 24-25 0.17806560506529934 2312 26-27 0.1742147197194086 2312 28-29 0.09000054161931814 2312 30-31 0.2055799578451314 2312 32-33 0.10464281444536994 2312 34-35 0.10538237984572874 2312 36-37 0.18189789084631514 2312 38-39 0.3041684293929663 2312 40-41 0.5525760405021813 2312 42-43 0.4049308986021529 2312 44-45 0.12239360917538278 2312 46-47 0.38243305727937127 2312 48-49 0.3375702766937039 2312 50-51 0.4351442661995293 2312 52-53 0.1310198002741565 2312 54-55 -0.08772899489308728 2312 56-57 0.0061740743602456405 2312 58-59 0.10998935169931556 2312 60-61 0.20360067465922072 2312 62-63 -0.015867419759420187 2312 64-65 0.15587729808270012 2312 66-67 0.271240846578344 2312 68-69 0.06390697838986625 2312 70-71 0.23418570661734606 2312 72-73 0.20273139260898176 2312 74-75 0.5207333290663385 2312 76-77 0.5141421370848782 2312 78-79 0.6612723330463091 2312 80-81 0.6143657429984586 2312 82-83 0.7625730226417602 2312 84-85 0.7804181497001039 2312 86-87 0.7900310859743271 2312 88-89 1.0305284084557833 2312 90-91 1.440801688465747 2312 92-93 2.7316930031110793 2312 94-95 2.0142713162566928 2312 96-97 1.5918258430225372 2312 98-99 1.7444882378452782 2312 100-101 1.744590048285941 2313 1 -0.353782058750415 2313 2 -0.9846532542958712 2313 3 -0.33353891716301476 2313 4 -0.25451428910469076 2313 5 -1.0196197401669878 2313 6 -0.6237615772838438 2313 7 -1.3770416711417397 2313 8 -1.1751169387109712 2313 9 -1.3994404666370244 2313 10-11 -2.242550451821792 2313 12-13 -0.8038019439421475 2313 14-15 -0.36360305926529435 2313 16-17 -1.7964808005784079 2313 18-19 -1.3273518406730815 2313 20-21 -1.50830900368382 2313 22-23 -1.1216831691649958 2313 24-25 -0.43398674525721503 2313 26-27 -1.0044632294468911 2313 28-29 -2.364765407475616 2313 30-31 -0.693401673151115 2313 32-33 -0.44606634150827773 2313 34-35 -0.1530108899083089 2313 36-37 -0.15997424875454413 2313 38-39 -0.5730250116504791 2313 40-41 -0.39619576098054665 2313 42-43 -0.05170424605710622 2313 44-45 -0.6981163336389358 2313 46-47 -0.7910478644717038 2313 48-49 -0.29928486778711516 2313 50-51 -0.418683338157642 2313 52-53 -0.34741834373383895 2313 54-55 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76-77 -0.3225806039796879 2314 78-79 -0.16009879061001797 2314 80-81 -0.03584915326087312 2314 82-83 -0.003709281169872014 2314 84-85 -0.19187451680582868 2314 86-87 -0.07438579639226006 2314 88-89 0.26836602621884253 2314 90-91 0.8978378578170982 2314 92-93 1.7547063727153578 2314 94-95 1.0364657236770718 2314 96-97 0.6735104859462915 2314 98-99 0.7951189610958345 2314 100-101 0.9748443846504511 2315 1 -7.067517710903079 2315 2 -2.8787441264483995 2315 3 -1.271780165917903 2315 4 -0.7685954354404743 2315 5 -1.373861790089272 2315 6 -1.5458871995167556 2315 7 -2.8557858375490923 2315 8 -2.6846524596928383 2315 9 -3.473310951115611 2315 10-11 -2.1155716673933966 2315 12-13 -2.4795944156712864 2315 14-15 -4.3796324233090616 2315 16-17 -4.194404799632849 2315 18-19 -3.117967306794977 2315 20-21 -4.249438638602868 2315 22-23 -5.509618861783281 2315 24-25 -3.3432402841938895 2315 26-27 -4.067046240884817 2315 28-29 -2.492669847531239 2315 30-31 -2.4523324775005975 2315 32-33 -1.5768695062675704 2315 34-35 -2.1961544390021217 2315 36-37 -2.8057070697948916 2315 38-39 -1.9578553938876624 2315 40-41 -3.352847014553916 2315 42-43 -7.680818906106115 2315 44-45 -4.156375243988343 2315 46-47 -2.6545773844728444 2315 48-49 -5.52782883490223 2315 50-51 -6.696650938637234 2315 52-53 -6.868735357045473 2315 54-55 -4.372624785373944 2315 56-57 -3.6890247072042186 2315 58-59 -3.972534788208147 2315 60-61 -2.9981613883610763 2315 62-63 -3.0795341434343264 2315 64-65 -1.8878431365974144 2315 66-67 -2.532633094339552 2315 68-69 -1.9991483088160997 2315 70-71 -1.551698005820775 2315 72-73 -1.8332499425855922 2315 74-75 -2.4374792777663323 2315 76-77 -2.4182695887393955 2315 78-79 -1.713643656840123 2315 80-81 -1.3617652619335239 2315 82-83 -1.7005705040727612 2315 84-85 -1.631093292777809 2315 86-87 -1.9759702637342365 2315 88-89 -1.4034136650108877 2315 90-91 -0.44231905036102503 2315 92-93 0.313559021424588 2315 94-95 0.2180235522892886 2315 96-97 0.0019334499245395875 2315 98-99 -0.22338916054524205 2315 100-101 -0.1076673392884171 2316 1 -4.392971420675579 2316 2 -3.1188607713418683 2316 3 -4.862102162658921 2316 4 -4.426347089132751 2316 5 -4.153402484686197 2316 6 -4.399301636313137 2316 7 -3.141053079920475 2316 8 -1.7282086177113385 2316 9 -2.0649529427609394 2316 10-11 -3.3964396548956444 2316 12-13 -4.115518095857588 2316 14-15 -3.1939789230636606 2316 16-17 -3.1615989942765914 2316 18-19 -3.3190341851797953 2316 20-21 -5.899144668171591 2316 22-23 -6.882597849200039 2316 24-25 -4.610582593505811 2316 26-27 -4.234035724117835 2316 28-29 -4.79505283848242 2316 30-31 -6.344097512450077 2316 32-33 -4.564035053030331 2316 34-35 -6.236303770996727 2316 36-37 -9.271481828064744 2316 38-39 -6.125706339117805 2316 40-41 -5.688157642796625 2316 42-43 -4.831978032216664 2316 44-45 -3.6946525642537225 2316 46-47 -6.09259218521607 2316 48-49 -3.3223687584926545 2316 50-51 -4.558527978540219 2316 52-53 -5.61150832536887 2316 54-55 -7.026272778061248 2316 56-57 -5.096866940507706 2316 58-59 -5.854083614664763 2316 60-61 -6.418299810092396 2316 62-63 -6.77646017576139 2316 64-65 -5.436740934894768 2316 66-67 -6.76712774649349 2316 68-69 -5.501650629530776 2316 70-71 -6.410703058916958 2316 72-73 -8.274908828855036 2316 74-75 -7.966556847176523 2316 76-77 -7.240205314499779 2316 78-79 -6.11121238986048 2316 80-81 -6.212151127602052 2316 82-83 -5.193234474020489 2316 84-85 -6.536748757029471 2316 86-87 -5.225075315331107 2316 88-89 -5.2803281615317275 2316 90-91 -5.793306976289987 2316 92-93 -4.526234863168298 2316 94-95 -4.331622315935025 2316 96-97 -5.118950142391846 2316 98-99 -6.316976640392582 2316 100-101 -5.2930158625255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17807.0 3 7233.0 4 7719.0 5 8545.0 6 9349.0 7 10854.0 8 12998.0 9 16007.0 10 18824.0 11 22055.0 12 25197.0 13 27700.0 14 30236.0 15 33079.0 16 36608.0 17 40391.0 18 39732.0 19 40159.0 20 42692.0 21 47359.0 22 52057.0 23 57985.0 24 64924.0 25 72976.0 26 82238.0 27 92593.0 28 104958.0 29 119309.0 30 135301.0 31 154869.0 32 178043.0 33 205510.0 34 239369.0 35 286731.0 36 327669.0 37 285473.0 38 88620.0 39 1533.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.02219486579371 18.867345244884778 13.116435935828175 23.99402395349334 2 15.225859603869974 20.328630054977694 40.33567198668937 24.109838354462966 3 19.33474715151579 24.545273138561306 29.46417036037899 26.65580934954392 4 12.498960323990989 16.341537103036906 37.8848403895966 33.27466218337551 5 13.732609667578371 37.51002656217126 34.75111114033242 14.006252629917945 6 32.07078641935704 36.477994036381226 17.75760834696271 13.693611197299017 7 28.539579663923114 32.189923742012766 21.494640770903228 17.775855823160892 8 25.932469817259978 35.360746528097366 20.164943316143656 18.541840338499004 9 26.559682980133996 16.360404991826996 20.276675827399565 36.80323620063945 10-11 24.835169242132707 26.317614715057893 28.17463258950826 20.672583453301144 12-13 25.958598359712703 23.68928225103956 28.07844780829348 22.27367158095426 14-15 22.846304544744033 25.036435894768104 26.14870250081649 25.968557059671372 16-17 22.7805348523852 28.19588813286203 26.601366896849616 22.422210117903155 18-19 22.53011151108758 27.397246764406592 28.22989400504835 21.842747719457478 20-21 23.294355518112887 26.632206628721722 28.07860768076931 21.994830172396078 22-23 22.930348239894904 26.601885597319807 27.91278491798331 22.55498124480198 24-25 22.923164882478382 26.63229672660112 27.98560283957335 22.458935551347146 26-27 22.741470059699058 26.81001412886472 28.02557008785154 22.422945723584682 28-29 22.532175114184348 27.087890087030882 28.056107577894075 22.3238272208907 30-31 22.760065310992708 26.73572152481994 27.95634773482711 22.547865429360247 32-33 22.533331579328173 26.915735399551416 28.004281965099683 22.546651056020732 34-35 23.074890473406136 26.843420873489798 27.774372256902264 22.307316396201806 36-37 22.886020207567846 26.7127471784799 27.82921982410257 22.57201278984968 38-39 22.72251040312222 27.004512459083024 28.019815752763805 22.253161385030946 40-41 22.917036949104 26.71701028054762 27.99248084821136 22.37347192213702 42-43 22.636980175098913 26.864881697743918 27.722865718915735 22.775272408241435 44-45 22.88967444270689 26.867091396201896 27.60965018998405 22.633583971107168 46-47 22.886869203091223 27.0145033917764 27.380746838677595 22.717880566454777 48-49 22.929310621291922 27.049465707859994 27.376068481491988 22.645155189356096 50-51 22.576728488062265 27.172756699145378 27.565774324493248 22.68474048829911 52-53 22.93449406993304 27.20788938568842 27.1822781287103 22.675338415668232 54-55 22.67362945583526 26.932213470852144 27.410768154779724 22.983388918532867 56-57 22.795519599245626 27.262946220161343 27.265495004300867 22.676039176292164 58-59 23.009576800613626 27.004390908410798 27.273748188267287 22.71228410270829 60-61 22.86255875235137 27.042597314709056 27.55074442252401 22.54409951041557 62-63 22.758469997104697 27.054519017017185 27.51781697042696 22.669194015451158 64-65 22.561722281174305 27.045278760236357 27.468615893718574 22.92438306487076 66-67 22.59061593731704 27.250258167522727 27.15923983249075 22.99988606266948 68-69 22.549746064121294 27.08177522807044 27.397980514730847 22.970498193077418 70-71 22.581162291699208 26.992790141319805 27.325167092790963 23.10088047419002 72-73 22.62990479012992 26.79688051211467 27.470262025733028 23.102952672022383 74-75 22.464393640826298 27.20178132132539 27.18028295599069 23.153542081857616 76-77 22.614022941199792 26.874905108021817 27.30461052877799 23.206461422000398 78-79 22.420008201376866 27.036679366793177 27.319533110386157 23.223779321443804 80-81 22.5277824522582 27.00781545710579 27.22253449396433 23.241867596671682 82-83 22.611677924617595 26.97198652895135 27.21460424065295 23.201731305778097 84-85 22.474181501462702 26.9118582543215 27.076103967355447 23.53785627686035 86-87 22.39813683678014 26.840385158110998 27.42371734020655 23.337760664902312 88-89 22.476016641249025 26.73236267605576 27.190883667036182 23.600737015659032 90-91 22.189421841562908 26.69874514257902 27.601232184928502 23.510600830929558 92-93 21.949336419404005 26.734408097402095 28.00089028889974 23.31536519429416 94-95 22.37927054284644 26.613127017832678 27.270759321118305 23.736843118202575 96-97 22.354489542264847 26.50570081672909 27.3251114787082 23.814698162297866 98-99 22.240415201410595 26.504005965673684 27.06453840073347 24.191040432182255 100-101 22.225641307494044 26.426876160420626 27.314567601541484 24.032914930543843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2281.0 1 1316.0 2 365.5 3 381.0 4 344.5 5 328.5 6 431.0 7 731.5 8 1149.0 9 1369.0 10 1275.5 11 1177.0 12 1283.5 13 1477.0 14 1621.5 15 1800.5 16 1997.5 17 2167.0 18 2417.5 19 2742.0 20 3189.0 21 3744.5 22 4549.5 23 5809.0 24 7315.0 25 9367.0 26 12552.5 27 16544.0 28 20814.5 29 25717.0 30 31405.5 31 37115.0 32 43013.5 33 49351.5 34 56577.5 35 63992.5 36 72053.5 37 80066.5 38 84843.0 39 86876.5 40 88872.0 41 93592.0 42 98212.0 43 101917.5 44 107345.0 45 111815.5 46 114382.0 47 119398.5 48 129216.0 49 131738.0 50 125772.5 51 120909.5 52 115813.5 53 111677.0 54 107152.0 55 99239.5 56 90773.5 57 82313.0 58 72964.0 59 63560.0 60 53391.0 61 42484.5 62 32839.0 63 25302.0 64 18991.5 65 13696.5 66 9768.5 67 7025.5 68 4784.0 69 3103.0 70 2050.0 71 1384.0 72 916.0 73 599.5 74 412.0 75 278.5 76 177.0 77 109.0 78 74.0 79 47.5 80 33.5 81 24.0 82 16.0 83 17.5 84 19.5 85 12.0 86 10.0 87 12.0 88 7.5 89 6.0 90 6.0 91 6.0 92 5.0 93 1.5 94 2.0 95 3.5 96 2.5 97 3.5 98 3.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08398194634483112 2 0.09600282720607796 3 0.09265274565458295 4 0.09488613335557962 5 0.09137183212018779 6 0.07403023350068413 7 0.08736487183310551 8 0.08427754177584539 9 0.07780728623031088 10-11 0.08429396374423506 12-13 0.0838177266609343 14-15 0.08918771032436015 16-17 0.08634670979294526 18-19 0.08925339819791887 20-21 0.09900804742138969 22-23 0.1076788467311415 24-25 0.13017694342500513 26-27 0.1352513316574167 28-29 0.13513637787868896 30-31 0.13704132621189202 32-33 0.13275519246218512 34-35 0.12769722619816323 36-37 0.13168776451685582 38-39 0.12441283252022693 40-41 0.1204551381383137 42-43 0.12079999947449702 44-45 0.11147232142915793 46-47 0.13022620933017418 48-49 0.14663175575146598 50-51 0.1564028269433265 52-53 0.16497509444274022 54-55 0.1617235447015833 56-57 0.13257455080989863 58-59 0.12347678032201509 60-61 0.11999532302340261 62-63 0.1172692762707155 64-65 0.1036061985705005 66-67 0.11680946115580441 68-69 0.11434616589735219 70-71 0.12240935237668579 72-73 0.1485695480214484 74-75 0.16211767194293564 76-77 0.1662395860087457 78-79 0.16318509988826493 80-81 0.16909700850855025 82-83 0.16903132063499154 84-85 0.1458270793003716 86-87 0.14766633976001592 88-89 0.16903132063499154 90-91 0.2621767253412649 92-93 0.5409889046612772 94-95 0.1670278404914504 96-97 0.1788188137952417 98-99 0.196538117687708 100-101 0.20371451787399883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3044702.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.540920496105244 #Duplication Level Percentage of deduplicated Percentage of total 1 77.4170993469184 45.32068257907031 2 12.49913344338238 14.63421554358516 3 4.346942011222255 7.634219600404258 4 1.974083163117995 4.62258582019162 5 1.0865009646138026 3.180238329419914 6 0.6571748232152184 2.308297144673047 7 0.432819236522136 1.7736345560099134 8 0.29874282834194643 1.3990944130198002 9 0.19874262085581598 1.0471118370037118 >10 0.993394055521022 10.054187782724261 >50 0.05820845227257611 2.3439313114974194 >100 0.033977955219758924 3.6745010190974425 >500 0.002272213426648774 0.9089083420498362 >1k 8.520800345236353E-4 0.9058821943281515 >5k 5.6805335634909015E-5 0.19250952692506404 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5789 0.19013355001573226 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4171 0.13699206030672295 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3900 0.1280913534395156 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.6421968389681487E-5 14-15 0.0 0.0 0.0 3.2843936779362974E-5 3.2843936779362974E-5 16-17 0.0 1.6421968389681487E-5 0.0 6.568787355872595E-5 3.2843936779362974E-5 18-19 0.0 3.2843936779362974E-5 0.0 6.568787355872595E-5 3.2843936779362974E-5 20-21 0.0 3.2843936779362974E-5 0.0 1.1495377872777041E-4 3.2843936779362974E-5 22-23 0.0 3.2843936779362974E-5 0.0 2.1348558906585932E-4 4.9265905169044465E-5 24-25 0.0 3.2843936779362974E-5 0.0 2.9559543101426676E-4 6.568787355872595E-5 26-27 0.0 3.2843936779362974E-5 0.0 6.07612830418215E-4 6.568787355872595E-5 28-29 0.0 3.2843936779362974E-5 0.0 0.0023483414797244523 6.568787355872595E-5 30-31 0.0 3.2843936779362974E-5 0.0 0.007012180502393994 6.568787355872595E-5 32-33 0.0 3.2843936779362974E-5 0.0 0.01801489932348059 8.210984194840743E-5 34-35 0.0 3.2843936779362974E-5 0.0 0.03404274047180972 1.6421968389681487E-4 36-37 0.0 3.2843936779362974E-5 0.0 0.05852789534082481 1.6421968389681487E-4 38-39 0.0 3.2843936779362974E-5 0.0 0.0979241975076707 1.6421968389681487E-4 40-41 0.0 3.2843936779362974E-5 0.0 0.14242773184370755 1.8064165228649635E-4 42-43 0.0 3.2843936779362974E-5 0.0 0.18817933577736015 1.9706362067617783E-4 44-45 0.0 3.2843936779362974E-5 0.0 0.24291375642016855 1.9706362067617783E-4 46-47 0.0 3.2843936779362974E-5 0.0 0.3080268610852556 1.9706362067617783E-4 48-49 0.0 3.2843936779362974E-5 0.0 0.3794295796435907 1.9706362067617783E-4 50-51 0.0 3.2843936779362974E-5 0.0 0.458764108934142 1.9706362067617783E-4 52-53 0.0 3.2843936779362974E-5 0.0 0.5357667187133585 2.463295258452223E-4 54-55 0.0 3.2843936779362974E-5 0.0 0.6222940701585902 2.9559543101426676E-4 56-57 0.0 3.2843936779362974E-5 0.0 0.7235847711861456 2.9559543101426676E-4 58-59 0.0 3.2843936779362974E-5 0.0 0.8406241398994057 2.9559543101426676E-4 60-61 0.0 3.2843936779362974E-5 0.0 0.9672703601206292 2.9559543101426676E-4 62-63 0.0 3.2843936779362974E-5 0.0 1.0964784074106433 2.9559543101426676E-4 64-65 0.0 3.2843936779362974E-5 0.0 1.231105704269252 2.9559543101426676E-4 66-67 0.0 3.2843936779362974E-5 0.0 1.3855871609109855 2.9559543101426676E-4 68-69 0.0 3.2843936779362974E-5 0.0 1.5615813961432021 2.9559543101426676E-4 70-71 0.0 3.2843936779362974E-5 0.0 1.7572327275378674 2.9559543101426676E-4 72-73 0.0 3.2843936779362974E-5 0.0 1.9652498011299628 3.2843936779362973E-4 74-75 0.0 3.2843936779362974E-5 0.0 2.176534846431605 3.2843936779362973E-4 76-77 0.0 3.2843936779362974E-5 0.0 2.4104822081110076 3.2843936779362973E-4 78-79 0.0 6.568787355872595E-5 0.0 2.6783080905783225 3.4486133618331124E-4 80-81 0.0 6.568787355872595E-5 0.0 2.971538758144475 3.612833045729927E-4 82-83 0.0 6.568787355872595E-5 0.0 3.2674462065581458 3.612833045729927E-4 84-85 0.0 6.568787355872595E-5 0.0 3.555389000302821 3.612833045729927E-4 86-87 0.0 6.568787355872595E-5 0.0 3.8680961223791357 3.612833045729927E-4 88-89 0.0 6.568787355872595E-5 0.0 4.21387708879227 3.612833045729927E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9125 0.0 27.35484 1 TACATGG 9100 0.0 26.80509 2 GTATCAA 12195 0.0 25.76069 1 ACATGGG 9255 0.0 25.074253 3 ATCAACG 14040 0.0 22.241003 3 CAACGCA 14065 0.0 22.201105 5 TCAACGC 14040 0.0 22.173037 4 AACGCAG 14395 0.0 21.820433 6 TATCAAC 14405 0.0 21.776285 2 CATGGGA 5870 0.0 20.938719 4 AGTACAT 6310 0.0 19.93022 2 GAGTACA 7120 0.0 19.395203 1 ACGCAGA 15830 0.0 19.393114 7 GGTATCA 9050 0.0 19.191715 1 CGCAGAG 15915 0.0 18.991398 8 AGAGTAC 10285 0.0 18.84615 10-11 GAGTACT 6240 0.0 18.402178 12-13 CATGGGG 4600 0.0 17.537977 4 GCAGAGT 16380 0.0 17.235918 9 GTACTTT 6790 0.0 16.703026 14-15 >>END_MODULE