FastQCFastQC Report
Fri 27 May 2016
SRR939621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044702
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61070.20057792191156965No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47540.15614007544909156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46280.15200173941489184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33200.10904187010748506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95750.024.2776171
GTATCAA118950.024.1711
TACATGG92300.024.10812
GGTATCA85900.022.624281
ACATGGG93400.022.4623833
TCAACGC137650.020.6067244
ATCAACG138350.020.434463
CAACGCA139400.020.3142855
AACGCAG140500.020.1612036
TATCAAC144400.019.6124972
CATGGGG44800.018.4800244
AGAGTAC113950.018.44790610-11
CATGGGA59000.018.417384
ACGCAGA157450.017.9311167
GAGTACT63350.017.90766112-13
CGCAGAG156550.017.6741168
AGTACTT69000.017.5986712-13
GTACTTT70300.016.27091614-15
AGTACAT70700.016.102732
GAGTACA78850.016.0534531
Produced by FastQC (version 0.11.5)