FastQCFastQC Report
Fri 27 May 2016
SRR939620_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939620_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3111226
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113750.029.5208241
GTACATG78500.029.1661321
TACATGG94050.026.5708272
ACATGGG91450.025.62163
TATCAAC142250.022.548922
GGTATCA86950.022.2356591
ATCAACG148750.021.7223763
TCAACGC148900.021.6983974
CAACGCA147750.021.6927625
AACGCAG152000.021.272556
CATGGGG44000.020.5043964
CATGGGA57150.020.2495844
ACGCAGA158050.019.89087
GAGTACT60750.018.91956912-13
AGAGTAC107450.018.77968410-11
GTCTTAG12000.018.6555211
CGCAGAG166050.018.6476258
GAGTACA69100.018.1131111
AGTACAT66800.017.3259812
GCAGAGT168000.017.0822839
Produced by FastQC (version 0.11.5)