##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939620_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3111226 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.019427068300406 2.0 2.0 31.0 2.0 33.0 2 22.37840452606143 16.0 16.0 31.0 16.0 34.0 3 26.85303478435832 28.0 25.0 31.0 16.0 34.0 4 32.07752731559842 35.0 32.0 35.0 25.0 37.0 5 32.69116483341294 35.0 33.0 35.0 26.0 37.0 6 33.07172606554458 35.0 33.0 37.0 26.0 37.0 7 33.22537385583689 35.0 33.0 37.0 26.0 37.0 8 33.15303806280868 35.0 33.0 37.0 26.0 37.0 9 34.50165401034833 37.0 33.0 39.0 27.0 39.0 10-11 34.33378497736905 37.0 33.0 39.0 26.5 39.0 12-13 34.318007435011154 37.0 33.0 39.0 27.0 39.0 14-15 35.01339664813807 38.0 33.0 40.0 25.5 41.0 16-17 34.61078703379311 38.0 33.0 40.0 25.0 41.0 18-19 34.82213651467299 38.0 33.0 40.0 25.0 41.0 20-21 34.80706785685129 38.0 33.0 40.0 25.0 41.0 22-23 34.61726840158832 38.0 33.0 40.0 24.5 41.0 24-25 34.429782664454464 38.0 33.0 40.0 24.0 41.0 26-27 34.53695376035042 38.0 33.0 40.0 24.0 41.0 28-29 34.28799048992262 38.0 33.0 40.0 23.5 41.0 30-31 34.169214322585375 38.0 33.0 40.0 23.5 41.0 32-33 33.89471481660284 38.0 32.0 40.0 21.0 41.0 34-35 33.629477575720955 38.0 32.0 40.0 19.0 41.0 36-37 33.36968722297898 37.5 31.0 40.0 18.0 41.0 38-39 33.18481556788224 37.0 31.0 40.0 17.5 41.0 40-41 32.82082738444588 37.0 30.5 40.0 15.5 41.0 42-43 32.58171408955826 37.0 30.0 40.0 15.0 41.0 44-45 32.26910356238987 37.0 30.0 39.5 14.0 41.0 46-47 31.970868075800343 36.5 30.0 39.0 11.0 40.5 48-49 31.634074798809216 36.0 30.0 39.0 9.0 40.0 50-51 30.98997999502447 35.5 28.5 38.5 8.5 40.0 52-53 31.048251396716278 35.5 29.0 38.5 8.0 40.0 54-55 31.158839634279218 36.0 29.0 39.0 7.5 40.0 56-57 30.859695663381572 36.0 28.5 39.0 4.5 40.0 58-59 30.42182727966403 35.0 28.0 39.0 2.0 40.0 60-61 29.82918020098829 34.5 27.0 38.0 2.0 40.0 62-63 29.422547895909844 34.0 26.5 38.0 2.0 40.0 64-65 28.83277315759125 34.0 26.0 38.0 2.0 39.5 66-67 28.219866059231954 33.5 25.5 37.0 2.0 39.0 68-69 27.846146824435127 33.0 25.0 36.5 2.0 39.0 70-71 27.298093098990556 32.5 23.5 36.0 2.0 39.0 72-73 26.69099528610265 32.0 22.0 35.5 2.0 38.0 74-75 26.109802855851683 31.5 21.5 35.0 2.0 38.0 76-77 25.453277261118288 31.0 19.5 35.0 2.0 37.0 78-79 24.77356755825517 31.0 17.5 34.0 2.0 36.0 80-81 24.013767884428837 30.0 10.5 34.0 2.0 36.0 82-83 23.307488269897462 29.5 7.0 34.0 2.0 35.0 84-85 22.59210034886569 29.0 2.0 34.0 2.0 35.0 86-87 21.897358147559835 29.0 2.0 34.0 2.0 35.0 88-89 21.073775900561387 28.5 2.0 33.0 2.0 34.5 90-91 20.089693259184642 26.5 2.0 32.5 2.0 34.0 92-93 18.993062863321406 25.0 2.0 32.0 2.0 34.0 94-95 17.96423998128069 23.5 2.0 32.0 2.0 34.0 96-97 17.02084435524774 20.0 2.0 31.0 2.0 34.0 98-99 15.943168866549712 10.0 2.0 31.0 2.0 34.0 100-101 14.427878109786946 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.823052082433215 1101 2 8.0680404215666 1101 3 3.87875122740585 1101 4 2.454207332355516 1101 5 1.6606117128324058 1101 6 1.528748020652266 1101 7 1.1571294276689486 1101 8 1.2155125865516894 1101 9 1.2257583756226325 1101 10-11 1.1902028882386517 1101 12-13 0.9725812057515526 1101 14-15 1.0832941277108787 1101 16-17 1.160074302312971 1101 18-19 1.2224603969580983 1101 20-21 1.1323036628215633 1101 22-23 1.1010186273688944 1101 24-25 1.2353584564397977 1101 26-27 1.31693390487996 1101 28-29 1.4021533036879106 1101 30-31 1.4399566169529194 1101 32-33 1.1602824766952509 1101 34-35 1.3123675925545157 1101 36-37 1.3410806107978743 1101 38-39 1.1239616902041973 1101 40-41 1.2614267590845074 1101 42-43 1.2346123559175126 1101 44-45 0.9002390400542666 1101 46-47 0.7866056903618386 1101 48-49 0.9079635938551895 1101 50-51 0.9425740684620472 1101 52-53 1.1392581876769334 1101 54-55 1.2700900824112544 1101 56-57 0.9155602763992725 1101 58-59 1.031220312319853 1101 60-61 1.0727606061468293 1101 62-63 0.6451210326799739 1101 64-65 0.7070971419291006 1101 66-67 0.846838674917251 1101 68-69 0.5735029177846052 1101 70-71 0.3594680914326993 1101 72-73 0.5454192420757522 1101 74-75 0.216604534498277 1101 76-77 -0.010167847366542304 1101 78-79 0.07539781435569282 1101 80-81 -0.18550844283794632 1101 82-83 -0.07417652850635292 1101 84-85 -0.12296869381605902 1101 86-87 -0.14006313574004992 1101 88-89 -0.3052204875307396 1101 90-91 -0.3931672717428434 1101 92-93 -0.47372841145081424 1101 94-95 -0.4838815530630036 1101 96-97 -1.181189638843307 1101 98-99 -1.2081131715173203 1101 100-101 -1.2164288922064053 1102 1 14.818592168316567 1102 2 7.983478414145292 1102 3 3.9425890270037414 1102 4 2.292155349103986 1102 5 1.6788707705105494 1102 6 1.4206625313898655 1102 7 1.1608016150454006 1102 8 0.9658248921261574 1102 9 1.2265952729136984 1102 10-11 1.2420037460561062 1102 12-13 0.9935815670008736 1102 14-15 1.012484769557119 1102 16-17 1.225071193318918 1102 18-19 1.1295054210344233 1102 20-21 1.1834535551634602 1102 22-23 1.068580896609781 1102 24-25 0.8711093660496232 1102 26-27 1.0565760648352125 1102 28-29 1.3034912325626138 1102 30-31 1.3678568947211787 1102 32-33 1.2287937466399441 1102 34-35 1.182822514499442 1102 36-37 1.3413729578461613 1102 38-39 1.1715139674811041 1102 40-41 1.2266931174100861 1102 42-43 1.0353497871717323 1102 44-45 1.0807342437482603 1102 46-47 0.9552894836212644 1102 48-49 0.9248380287794156 1102 50-51 0.7597662612672131 1102 52-53 1.1026009069608058 1102 54-55 1.1441058322285862 1102 56-57 0.9303094004467773 1102 58-59 0.8879781513485341 1102 60-61 0.9697522937835537 1102 62-63 0.6390603086826196 1102 64-65 0.7883836830421238 1102 66-67 0.7863911485134842 1102 68-69 0.40063305045981323 1102 70-71 0.6960881857857224 1102 72-73 0.7931748604462321 1102 74-75 0.42814817408793715 1102 76-77 0.24161989225597225 1102 78-79 0.29423325612587803 1102 80-81 0.24125374533502608 1102 82-83 0.06915417574476024 1102 84-85 -0.17679860798169855 1102 86-87 -0.3197163617908636 1102 88-89 -0.19257420287389593 1102 90-91 0.05744149959395273 1102 92-93 0.12301088247821923 1102 94-95 -0.12261131806586789 1102 96-97 -0.7560033577535936 1102 98-99 -1.1196205138055007 1102 100-101 -1.0943513420828346 1103 1 14.858884292346662 1103 2 7.939716564035777 1103 3 3.869826551895102 1103 4 2.3693679651176964 1103 5 1.8598917472188248 1103 6 1.4025366809496305 1103 7 1.1200158678281227 1103 8 1.102801823212296 1103 9 1.1490239130080937 1103 10-11 1.091588195209063 1103 12-13 0.78574833806762 1103 14-15 1.0242641112340536 1103 16-17 1.1454633312223095 1103 18-19 1.0564034705046481 1103 20-21 0.9658657504939043 1103 22-23 1.1871287615931507 1103 24-25 1.3541438278847409 1103 26-27 1.32851164227975 1103 28-29 1.2897189179218032 1103 30-31 1.1602568142840752 1103 32-33 1.033016099187087 1103 34-35 1.0476475357648312 1103 36-37 1.1655788165823537 1103 38-39 1.118931536963217 1103 40-41 0.9897431271494312 1103 42-43 1.1894451780133934 1103 44-45 1.0787064999530571 1103 46-47 0.9080009778596434 1103 48-49 0.8871974214932727 1103 50-51 0.9702887556303139 1103 52-53 1.0936884509804976 1103 54-55 1.0784204036767697 1103 56-57 1.060742758864155 1103 58-59 0.7658677212902774 1103 60-61 0.9781210285527067 1103 62-63 0.738403551615292 1103 64-65 0.8464818008979016 1103 66-67 0.6648825078305229 1103 68-69 0.5497165368899068 1103 70-71 0.4763843526913156 1103 72-73 0.4482495275220906 1103 74-75 0.47700268101827703 1103 76-77 0.3418797888928893 1103 78-79 0.42318907317209664 1103 80-81 0.2987848446468284 1103 82-83 0.2482242197889768 1103 84-85 -0.0022474582495632944 1103 86-87 -0.20672385967833762 1103 88-89 -0.3470072298735758 1103 90-91 -0.16765049859550984 1103 92-93 0.03527522908087377 1103 94-95 -0.16799338381409612 1103 96-97 -0.5744623075028237 1103 98-99 -0.8617672982130333 1103 100-101 -0.7224851836833928 1104 1 14.905097883497028 1104 2 8.079774940859007 1104 3 4.040050469108177 1104 4 2.5078149855021934 1104 5 1.8970414584013042 1104 6 1.3921813461889343 1104 7 1.1521830586038035 1104 8 1.1902806358265963 1104 9 1.3346428355737743 1104 10-11 1.13592732998314 1104 12-13 0.9623451978740363 1104 14-15 1.1092861628420607 1104 16-17 1.3092376409595872 1104 18-19 1.1460801259085542 1104 20-21 1.1337569644195895 1104 22-23 1.1495946805681356 1104 24-25 1.2717165340714587 1104 26-27 1.22276533055755 1104 28-29 1.2482170807524398 1104 30-31 1.127605240841902 1104 32-33 1.259831244822486 1104 34-35 1.1591851824038386 1104 36-37 1.2727416862464764 1104 38-39 1.269659574565246 1104 40-41 1.3449095735787964 1104 42-43 1.2868904253482398 1104 44-45 1.0256188566594275 1104 46-47 1.1103178603031054 1104 48-49 0.9522329382424566 1104 50-51 0.9509026461918886 1104 52-53 0.9130408975659883 1104 54-55 0.8481438385980447 1104 56-57 0.8125015209632664 1104 58-59 0.8628310252958116 1104 60-61 0.747398413248721 1104 62-63 0.6433408397414375 1104 64-65 0.8684211993991688 1104 66-67 0.7316053489474328 1104 68-69 0.8452077357613987 1104 70-71 0.7134938496581782 1104 72-73 0.6273407838225502 1104 74-75 0.4980722815803027 1104 76-77 0.4009368387166994 1104 78-79 0.5002756124914249 1104 80-81 0.346010063769004 1104 82-83 0.04237099540341305 1104 84-85 -0.02611288768441611 1104 86-87 0.16414564554045086 1104 88-89 0.15890502647149063 1104 90-91 0.1410852556754172 1104 92-93 0.29429171621232086 1104 94-95 0.22425447235700702 1104 96-97 -0.19273600949667014 1104 98-99 -0.6662491969373292 1104 100-101 -0.622030839711762 1105 1 14.66400447272239 1105 2 7.897125513835128 1105 3 3.806481566491737 1105 4 2.4107622593892515 1105 5 1.7399280074511907 1105 6 1.3038379689871178 1105 7 1.0741479358391928 1105 8 1.122199154593801 1105 9 1.1294719918888205 1105 10-11 0.9339376857990729 1105 12-13 0.8199936456906798 1105 14-15 1.0632343963522075 1105 16-17 1.2384990109544347 1105 18-19 1.0930212573685196 1105 20-21 1.154854333989185 1105 22-23 0.9870170783948495 1105 24-25 1.1361396970786828 1105 26-27 1.1181875717111183 1105 28-29 1.255069258229554 1105 30-31 1.137460611933463 1105 32-33 0.828230968406281 1105 34-35 1.1819881211446344 1105 36-37 1.1125703109918845 1105 38-39 1.1042802156941889 1105 40-41 1.0808353930353647 1105 42-43 1.1683000361273557 1105 44-45 0.9854710103557878 1105 46-47 1.0163526095700988 1105 48-49 1.0971912799689996 1105 50-51 1.0112545103226864 1105 52-53 1.04457360868307 1105 54-55 1.1402967865713656 1105 56-57 1.1904288260170937 1105 58-59 0.9252392246176413 1105 60-61 0.8398993994502923 1105 62-63 0.7910960421295847 1105 64-65 0.7651865281889805 1105 66-67 0.6873511271653889 1105 68-69 0.6801408489129912 1105 70-71 0.5494379942786942 1105 72-73 0.6809107635669491 1105 74-75 0.7638644462437938 1105 76-77 0.6729200411160363 1105 78-79 0.6863218394660677 1105 80-81 0.5161537904079161 1105 82-83 0.635199666311447 1105 84-85 0.450355538477762 1105 86-87 0.5821302202287804 1105 88-89 0.5357532801253306 1105 90-91 0.4398976871309124 1105 92-93 0.6214626885930841 1105 94-95 0.5721438960795631 1105 96-97 0.26102897237401024 1105 98-99 -0.035342653874593566 1105 100-101 0.20719699243533185 1106 1 14.597779879888286 1106 2 7.8203361056804646 1106 3 3.543734392389016 1106 4 2.266890440493171 1106 5 1.6003008116124136 1106 6 1.058067186335066 1106 7 0.7287984993380263 1106 8 0.8613213232695145 1106 9 0.9974033972676537 1106 10-11 0.8736717762529906 1106 12-13 0.4320022560816952 1106 14-15 0.7691570863011066 1106 16-17 1.0249678262974058 1106 18-19 0.9000698245675238 1106 20-21 0.6971950004401748 1106 22-23 0.6797769902181727 1106 24-25 0.689062160143493 1106 26-27 0.6423853894223583 1106 28-29 0.7488726617709034 1106 30-31 0.6338763335999076 1106 32-33 0.6307665380884018 1106 34-35 0.7922366292417351 1106 36-37 0.7508055659773731 1106 38-39 0.9157540729056706 1106 40-41 0.8869132254800647 1106 42-43 0.6347719606225795 1106 44-45 0.5671844683633296 1106 46-47 0.6153148599801028 1106 48-49 0.6046709269261576 1106 50-51 0.6370895697957266 1106 52-53 0.48326098622518643 1106 54-55 0.5618769991981658 1106 56-57 0.5512188097850803 1106 58-59 0.55385466449966 1106 60-61 0.5205337925563782 1106 62-63 0.5376283570054774 1106 64-65 0.5664372967238016 1106 66-67 0.4927785935844433 1106 68-69 0.4575981996071583 1106 70-71 0.5796569934397802 1106 72-73 0.5233642768389686 1106 74-75 0.3737659757506293 1106 76-77 0.2528628929369212 1106 78-79 0.20794077644411146 1106 80-81 0.38684386815072713 1106 82-83 0.3269942703917188 1106 84-85 0.28369534921161943 1106 86-87 0.27659806501205964 1106 88-89 0.3906778901858168 1106 90-91 0.6794911304610132 1106 92-93 1.0701902120132658 1106 94-95 0.7534211274214968 1106 96-97 0.164589581935914 1106 98-99 -0.16663781186014326 1106 100-101 -0.10741122637055867 1107 1 14.667520574305076 1107 2 7.879990902406089 1107 3 3.7039123433518917 1107 4 2.08634403807244 1107 5 1.3824500486849942 1107 6 1.1710196355269886 1107 7 0.9067669894961057 1107 8 0.8600891555236316 1107 9 0.7700931616168916 1107 10-11 0.7334240947615243 1107 12-13 0.6071458746095644 1107 14-15 0.6154786011067515 1107 16-17 0.8488347928441371 1107 18-19 0.8135690197148477 1107 20-21 0.824840217455943 1107 22-23 0.6813779319238407 1107 24-25 0.8622159066345318 1107 26-27 0.6360778473639002 1107 28-29 0.8378330697498768 1107 30-31 0.9034302923813655 1107 32-33 0.8283801288811148 1107 34-35 0.7852747938411184 1107 36-37 0.6983897109405959 1107 38-39 0.6744425206056732 1107 40-41 0.9100373158142645 1107 42-43 0.6331599767308447 1107 44-45 0.5593022755183839 1107 46-47 0.5004838369758957 1107 48-49 0.5118408461884947 1107 50-51 0.5667897551642369 1107 52-53 0.5708300787608103 1107 54-55 0.5505689243104115 1107 56-57 0.4439281976763958 1107 58-59 0.7611767906568119 1107 60-61 0.6411917596163121 1107 62-63 0.33234815890515534 1107 64-65 0.31921431888108387 1107 66-67 0.29919136942876534 1107 68-69 0.4059777325121736 1107 70-71 0.28515785998847676 1107 72-73 0.28555502552021395 1107 74-75 0.26190880625201984 1107 76-77 0.050121170720462516 1107 78-79 0.21729385858114725 1107 80-81 0.4238216954411236 1107 82-83 0.2906851088126352 1107 84-85 0.2254553544838238 1107 86-87 0.1961921963180231 1107 88-89 0.4417075126902823 1107 90-91 0.8924850842506906 1107 92-93 1.0386398820797567 1107 94-95 0.8057270864160735 1107 96-97 0.3505284271546145 1107 98-99 0.27261666881357804 1107 100-101 0.2614868265590218 1108 1 14.232298726861199 1108 2 7.521451348216061 1108 3 3.371729957563044 1108 4 -0.14248144926695971 1108 5 0.3280506008336843 1108 6 0.5649649591698918 1108 7 0.6894847215676236 1108 8 0.4954440307266239 1108 9 0.6832426286013771 1108 10-11 0.6787275608582277 1108 12-13 0.34163939159356715 1108 14-15 0.48918224141972644 1108 16-17 0.6405451179411656 1108 18-19 0.5419225263738952 1108 20-21 0.4327890783013828 1108 22-23 -0.20128907983575317 1108 24-25 0.331963049529449 1108 26-27 0.35502948495509656 1108 28-29 0.3207958456328939 1108 30-31 0.4741619067901084 1108 32-33 0.6816214455497942 1108 34-35 0.6484849303893796 1108 36-37 0.5449319806502047 1108 38-39 0.46288602972767023 1108 40-41 0.8160735895341844 1108 42-43 0.7460513719499176 1108 44-45 0.535055409532724 1108 46-47 0.39872908116393546 1108 48-49 0.4110702314082211 1108 50-51 0.4800745458890283 1108 52-53 0.6626070568877616 1108 54-55 0.465541696060658 1108 56-57 0.708295908805777 1108 58-59 0.4934594690788998 1108 60-61 0.4701269844207019 1108 62-63 0.4910550762094772 1108 64-65 0.21659578711236094 1108 66-67 0.3974804981665194 1108 68-69 0.3953589064404035 1108 70-71 0.31503631561411893 1108 72-73 0.03514635826076784 1108 74-75 0.04349515880062782 1108 76-77 0.004103184838662344 1108 78-79 0.226877057982243 1108 80-81 0.22038115022212068 1108 82-83 0.35485778329676876 1108 84-85 0.07487812393809179 1108 86-87 0.14234178366758599 1108 88-89 0.2211910421560752 1108 90-91 0.5024194470185002 1108 92-93 0.8970033240756194 1108 94-95 0.673290368528157 1108 96-97 0.1780445790344629 1108 98-99 -0.028583277056926093 1108 100-101 -0.10780174627297967 1109 1 14.152559366136302 1109 2 6.400503840044003 1109 3 2.9082289887017936 1109 4 -3.935900125584112 1109 5 -0.7559384037177068 1109 6 -0.0015867960461903863 1109 7 0.06393434251582164 1109 8 0.22207280954547315 1109 9 0.3888731163597541 1109 10-11 0.400552937050243 1109 12-13 0.2897399729103185 1109 14-15 0.3364838155159475 1109 16-17 0.5408002456263219 1109 18-19 0.4401781428226954 1109 20-21 0.4174209283584105 1109 22-23 0.5061843241429855 1109 24-25 0.6376635587448902 1109 26-27 0.559900915853973 1109 28-29 0.5997446918387368 1109 30-31 0.47604884640180245 1109 32-33 0.4603301562883857 1109 34-35 0.4507043852619361 1109 36-37 0.5152095186680441 1109 38-39 0.6539508411723745 1109 40-41 0.5655974782901936 1109 42-43 0.459780343847747 1109 44-45 0.4223563943394524 1109 46-47 0.03762244749747623 1109 48-49 0.20338078350314603 1109 50-51 -1.182557740935497 1109 52-53 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0.24812804727099547 2312 24-25 0.20176981220366486 2312 26-27 0.42597834976482574 2312 28-29 -0.3461967325365549 2312 30-31 0.37080981868906093 2312 32-33 0.38598317233708457 2312 34-35 -0.44211690799931347 2312 36-37 0.1220600146026527 2312 38-39 0.4397777536069185 2312 40-41 0.6290034635611548 2312 42-43 0.5077048681336365 2312 44-45 0.5659126499266947 2312 46-47 0.513218846118221 2312 48-49 0.5702061634358877 2312 50-51 0.5670531738829432 2312 52-53 0.4807984650968713 2312 54-55 0.364502134037167 2312 56-57 0.39449745723311835 2312 58-59 0.43631221675773446 2312 60-61 0.5561925713184941 2312 62-63 0.417677026452445 2312 64-65 0.5543982762282731 2312 66-67 0.33520527089666885 2312 68-69 0.696671908762621 2312 70-71 0.5840664623227632 2312 72-73 0.32475874567947827 2312 74-75 0.8136787869420488 2312 76-77 0.8028063242603416 2312 78-79 0.778205485574091 2312 80-81 0.8866122848223483 2312 82-83 0.9276801018918057 2312 84-85 1.1364183750412522 2312 86-87 1.1050758494905004 2312 88-89 1.5408865827359115 2312 90-91 1.9004819741496952 2312 92-93 2.332042751233363 2312 94-95 2.432903388130047 2312 96-97 2.260509629840918 2312 98-99 2.271504764987469 2312 100-101 2.1265955092091033 2313 1 -14.0465530598992 2313 2 -7.269779706959781 2313 3 -3.6168885914867808 2313 4 -1.1028855975937688 2313 5 -0.4335814503262725 2313 6 -0.8717459472398232 2313 7 -0.49319608361912515 2313 8 -0.21653370018757556 2313 9 -2.6070069904268927 2313 10-11 -0.6220205730720281 2313 12-13 -0.7957458394517616 2313 14-15 -0.9832898029704182 2313 16-17 -0.9690685172226168 2313 18-19 -0.225479483214869 2313 20-21 -1.1820758301491168 2313 22-23 -2.097739587800895 2313 24-25 -1.317464274881928 2313 26-27 -0.45306724019032885 2313 28-29 0.050952128496319915 2313 30-31 -1.366566106128893 2313 32-33 -0.4207684104592673 2313 34-35 -1.3162871307894974 2313 36-37 -1.4063473493352276 2313 38-39 -0.7387525858421782 2313 40-41 -0.431799640116445 2313 42-43 -0.1493408010395143 2313 44-45 -0.4483113994052701 2313 46-47 -0.9777262036952976 2313 48-49 -0.3251601676661373 2313 50-51 -1.1934233848874243 2313 52-53 -0.3379322958753441 2313 54-55 -0.9472451021929729 2313 56-57 -0.5260725370756951 2313 58-59 -0.2852490448096532 2313 60-61 -1.6660018504839655 2313 62-63 -0.5072256917670686 2313 64-65 -0.3070231447340568 2313 66-67 -1.5446509692922916 2313 68-69 -0.39877622534093504 2313 70-71 -0.06682947233489855 2313 72-73 -0.980919725163087 2313 74-75 -0.5119729165575073 2313 76-77 0.05899226973751226 2313 78-79 0.022073283251337728 2313 80-81 -0.09903128840947417 2313 82-83 0.06939825677966027 2313 84-85 0.29671469202996903 2313 86-87 0.18694055766600215 2313 88-89 0.40806894464122934 2313 90-91 0.7712267275211531 2313 92-93 1.4044480388259473 2313 94-95 1.5873245234906683 2313 96-97 1.156653506394271 2313 98-99 0.8344159011611154 2313 100-101 0.9743655852375284 2314 1 -14.0465530598992 2314 2 -7.176345995162134 2314 3 -3.6114732959842044 2314 4 -1.426948515930924 2314 5 -1.4435241079157919 2314 6 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-2.4054819760655555 2314 70-71 -3.1533324038966697 2314 72-73 -3.1077485028894465 2314 74-75 -2.0980741843853608 2314 76-77 -0.8991903395871397 2314 78-79 -0.4724780422261148 2314 80-81 -0.3209892004685422 2314 82-83 -0.4147244417519289 2314 84-85 -0.2479107408783321 2314 86-87 -0.1881025068472333 2314 88-89 0.12453257562475173 2314 90-91 0.6328150866351301 2314 92-93 1.04278605709208 2314 94-95 0.9592448047805604 2314 96-97 0.6495638540363302 2314 98-99 0.782429407565381 2314 100-101 0.8365099324232688 2315 1 -14.0465530598992 2315 2 -7.3694092139349845 2315 3 -4.787315382892999 2315 4 -3.005626004101199 2315 5 -3.739921227616499 2315 6 -2.744277718569041 2315 7 -3.2666054473646255 2315 8 -3.5632344494527963 2315 9 -2.441320025985945 2315 10-11 -4.850815539334398 2315 12-13 -4.584932203652613 2315 14-15 -3.639771361110917 2315 16-17 -4.633617095274531 2315 18-19 -1.673770868734593 2315 20-21 -3.381872990392523 2315 22-23 -4.39366312963142 2315 24-25 -4.879992734541652 2315 26-27 -4.6592600621467 2315 28-29 -2.5950252279635038 2315 30-31 -3.5837702291574196 2315 32-33 -4.595681479135017 2315 34-35 -3.872275043195838 2315 36-37 -5.1151015406669345 2315 38-39 -3.6005317802785797 2315 40-41 -4.805712998563266 2315 42-43 -3.5599464225984825 2315 44-45 -1.9220508952035864 2315 46-47 -1.3376270605897815 2315 48-49 -0.9682656857354353 2315 50-51 -0.7725308899584178 2315 52-53 -0.6272997670017268 2315 54-55 -2.379521461012363 2315 56-57 -4.426810418435135 2315 58-59 -3.855559927783535 2315 60-61 -4.790344691068473 2315 62-63 -2.6058513489264854 2315 64-65 -3.146381301789983 2315 66-67 -1.8611844792479957 2315 68-69 -1.324905297659516 2315 70-71 -1.8759686452454467 2315 72-73 -1.956868725452857 2315 74-75 -2.8054230552336676 2315 76-77 -2.717045731669945 2315 78-79 -1.8654075711593947 2315 80-81 -1.3494659450307438 2315 82-83 -1.0411959809154148 2315 84-85 -0.7837795746422209 2315 86-87 -1.5038219542353097 2315 88-89 -2.150009459134072 2315 90-91 -1.5641108557880337 2315 92-93 -0.6046694012535312 2315 94-95 -0.3283417430159332 2315 96-97 -1.3382082314042982 2315 98-99 -0.7215461388272928 2315 100-101 -0.21867887392544816 2316 1 -14.0465530598992 2316 2 -7.310407162453464 2316 3 -5.276051793534723 2316 4 -3.080109840057961 2316 5 -2.336332832528985 2316 6 -1.5250527091678023 2316 7 -4.8666716343066625 2316 8 -3.310600626172487 2316 9 -1.5601345361121588 2316 10-11 -4.745799724216468 2316 12-13 -3.8522461227646794 2316 14-15 -2.192486529563787 2316 16-17 -3.4412044400802664 2316 18-19 -2.676823596127427 2316 20-21 -3.597875284331927 2316 22-23 -3.2261495979353114 2316 24-25 -3.6575479619815354 2316 26-27 -4.188469409580726 2316 28-29 -3.8433342503120684 2316 30-31 -2.963444693471274 2316 32-33 -3.1644463813455133 2316 34-35 -4.501460071034209 2316 36-37 -2.6886340431238054 2316 38-39 -3.5184149495315253 2316 40-41 -3.328748296297487 2316 42-43 -6.900356180107387 2316 44-45 -3.841574760698137 2316 46-47 -5.146387715174395 2316 48-49 -4.648910092283792 2316 50-51 -3.5303162051296795 2316 52-53 -5.025870354166809 2316 54-55 -5.078863171259329 2316 56-57 -5.156633872563173 2316 58-59 -3.8613355549726194 2316 60-61 -3.508581417053062 2316 62-63 -2.752592984406377 2316 64-65 -2.861972533671608 2316 66-67 -3.229519885959096 2316 68-69 -3.217944283927249 2316 70-71 -3.2083904396625655 2316 72-73 -4.160652175506719 2316 74-75 -4.821733837154799 2316 76-77 -4.344971510035396 2316 78-79 -4.642617794320287 2316 80-81 -4.50451674493878 2316 82-83 -4.185172184984502 2316 84-85 -3.834896117170697 2316 86-87 -3.906370585123259 2316 88-89 -3.7731378374451268 2316 90-91 -2.851302123318714 2316 92-93 -2.838744679074466 2316 94-95 -2.162349339291456 2316 96-97 -2.2698528689703608 2316 98-99 -2.310463092021113 2316 100-101 -2.023518408878685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24874.0 3 7779.0 4 8644.0 5 10070.0 6 11337.0 7 13956.0 8 16750.0 9 20043.0 10 23868.0 11 27442.0 12 31031.0 13 33357.0 14 36308.0 15 39366.0 16 42741.0 17 45372.0 18 45026.0 19 47023.0 20 51439.0 21 55983.0 22 61549.0 23 68121.0 24 75484.0 25 84193.0 26 94446.0 27 105043.0 28 118849.0 29 132503.0 30 150012.0 31 169858.0 32 193791.0 33 222377.0 34 255307.0 35 294039.0 36 301647.0 37 171378.0 38 20130.0 39 90.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.00139635299748 18.67940746425482 13.138462667990062 24.180733514757634 2 15.310063741803074 20.31669848663481 40.37785072869603 23.99538704286608 3 19.29179921471916 24.57767503583061 29.540705311168015 26.589820438282214 4 12.508312286275622 16.31301276541754 38.02018005740428 33.15849489090255 5 13.69070565936948 37.47328315511377 34.84697144732735 13.989039738189401 6 32.021001430394136 36.38949722482938 17.919870891157437 13.669630453619053 7 28.468472354423223 32.15775749044531 21.63139595782 17.74237419731146 8 25.883888901943124 35.280170547691 20.263257086223728 18.57268346414214 9 26.63115614117526 16.338403506852135 20.26938649398467 36.76105385798793 10-11 24.845068790824566 26.269929065118973 28.24355515937218 20.64144698468428 12-13 25.96752715390687 23.68502996806464 28.124020878274703 22.223421999753786 14-15 22.893408106202106 25.008634199929126 26.19923645669816 25.89872123717061 16-17 22.752931085396753 28.2109496386785 26.644485552370096 22.391633723554655 18-19 22.559219129084305 27.345819435671093 28.28027343357085 21.814688001673755 20-21 23.303334378660388 26.604566297668363 28.130362801467925 21.961736522203328 22-23 22.96187951484424 26.597987577415733 27.930430178996506 22.509702728743523 24-25 22.949682765214046 26.614479770061557 28.04460517258429 22.39123229214011 26-27 22.769481900389454 26.763415011620207 28.11015416358601 22.35694892440433 28-29 22.5854182831409 27.083121963725915 28.053248070457183 22.278211682676 30-31 22.779511841717643 26.735314445234927 28.0055684042186 22.47960530882883 32-33 22.483074907184974 26.877156906513704 28.100533988369374 22.539234197931947 34-35 23.059086100201444 26.834844583572863 27.855763765098413 22.250305551127276 36-37 22.82261723551307 26.6746837884652 27.955437511693198 22.547261464328532 38-39 22.63210084772328 26.98390158147332 28.158941715220813 22.22505585558259 40-41 22.91667874183799 26.71349901112397 28.079490691813934 22.290331555224103 42-43 22.64316947993123 26.864217422389913 27.778075614021613 22.714537483657242 44-45 22.90371748205537 26.841278648687204 27.649909974955296 22.60509389430213 46-47 22.88954797914927 27.048629426008976 27.377576847410047 22.68424574743171 48-49 22.984148346696994 27.06843064943959 27.33542468189667 22.611996321966746 50-51 22.60670359457563 27.19028847969083 27.556211755806736 22.6467961699268 52-53 22.926944334074822 27.205644221836174 27.23305133492459 22.634360109164415 54-55 22.645734564325164 26.9314529926647 27.457483546643246 22.96532889636689 56-57 22.811930285600713 27.25065122296879 27.331875916757493 22.605542574673006 58-59 22.972869196131665 27.014935393303197 27.274250875775373 22.737944534789765 60-61 22.894283087042506 27.012676574975604 27.558359727098853 22.53468061088304 62-63 22.78702240221974 27.021719500146208 27.513728460518845 22.677529637115214 64-65 22.59059537831713 27.072853682772724 27.462936571160302 22.87361436774984 66-67 22.59521074959219 27.259964670176895 27.16868549453299 22.976139085697927 68-69 22.598957649101386 27.05018430793722 27.406290812423208 22.94456723053819 70-71 22.575351323917463 26.989016035403225 27.39386214461039 23.04177049606892 72-73 22.67519454339575 26.80150026983792 27.478309492416436 23.044995694349897 74-75 22.459201869531363 27.21770435634897 27.18588713893298 23.137206635186686 76-77 22.62133158615699 26.87748022545534 27.300285516409662 23.200902671978003 78-79 22.45973195662386 27.047150838996775 27.281812833580233 23.211304370799134 80-81 22.536345540554734 27.016185936574686 27.219009550591842 23.22845897227873 82-83 22.608405561826178 26.961155200556192 27.248946956979918 23.181492280637716 84-85 22.503113222754408 26.90600651904738 27.114370616910072 23.47650964128814 86-87 22.422366980683407 26.894870880996447 27.360927487757113 23.321834650563034 88-89 22.469567911941862 26.759380633465447 27.17487500783139 23.596176446761298 90-91 22.31523726888756 26.740473204387683 27.42454283168979 23.519746695034968 92-93 22.408892405863362 26.76238827688058 27.474350160864546 23.354369156391513 94-95 22.395672655048184 26.584699026598695 27.311830104960055 23.707798213393062 96-97 22.353426691027202 26.510885374752686 27.307592770864563 23.828095163355552 98-99 22.193440495481223 26.574335560622085 27.042620629943748 24.189603313952944 100-101 22.250879155237378 26.44042857004716 27.286077597738533 24.022614676976932 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3019.0 1 1905.5 2 758.5 3 604.5 4 473.5 5 454.5 6 548.5 7 1027.5 8 1366.0 9 1277.5 10 1202.5 11 1223.0 12 1338.0 13 1471.5 14 1659.5 15 1860.5 16 2009.0 17 2207.5 18 2490.5 19 2851.0 20 3413.5 21 4121.5 22 5053.0 23 6372.5 24 7881.5 25 10270.0 26 13918.0 27 18196.0 28 22731.5 29 28129.5 30 34033.0 31 40113.5 32 46263.0 33 53095.0 34 60923.0 35 68519.5 36 76514.5 37 83307.5 38 87486.0 39 90095.5 40 93626.0 41 98060.0 42 102070.0 43 107149.5 44 112150.0 45 115574.0 46 119310.0 47 127656.5 48 133978.0 49 131721.0 50 127002.5 51 122273.5 52 117035.0 53 112762.0 54 106491.5 55 97494.5 56 88734.0 57 79853.5 58 70433.5 59 60462.0 60 49742.5 61 39319.0 62 30508.5 63 22954.0 64 16930.0 65 12182.0 66 8674.5 67 6186.5 68 4101.0 69 2731.5 70 1822.0 71 1183.5 72 804.0 73 512.0 74 329.5 75 233.0 76 142.0 77 80.0 78 58.0 79 35.0 80 25.5 81 26.0 82 19.0 83 13.0 84 10.5 85 9.5 86 9.0 87 11.5 88 10.0 89 6.0 90 8.5 91 6.5 92 6.0 93 6.5 94 3.5 95 4.5 96 4.5 97 2.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.59511009486293 2 0.0832790674801509 3 0.11403864585857794 4 0.18908944576832412 5 0.11686711283590456 6 0.14110193216436223 7 0.1489766413626011 8 0.1455696243217304 9 0.13390219804025807 10-11 0.12209013424289974 12-13 0.1339825522157503 14-15 0.1412626405153467 16-17 0.13591105242756393 18-19 0.14266080316891155 20-21 0.17163651885141099 22-23 0.19008583754442782 24-25 0.21673128213765247 26-27 0.2090654937956934 28-29 0.20811731452488502 30-31 0.22259713694858552 32-33 0.21419209019209792 34-35 0.19866766348699835 36-37 0.18606812876981613 38-39 0.18232362419187806 40-41 0.18256468671835477 42-43 0.17653812355643725 44-45 0.1921750461072259 46-47 0.21787231142964222 48-49 0.22015437001362165 50-51 0.23013435860975706 52-53 0.25767976996849473 54-55 0.24440526017717773 56-57 0.22095791176854396 58-59 0.17837019875766016 60-61 0.18810912482731887 62-63 0.19278573784096686 64-65 0.18012191978339084 66-67 0.19019833339011694 68-69 0.17851483627354617 70-71 0.19195005441584764 72-73 0.21163682741144485 74-75 0.24300709752361288 76-77 0.24271782249184085 78-79 0.23784835945701147 80-81 0.24247675996536414 82-83 0.23288247141159146 84-85 0.20327999316025255 86-87 0.21430458603778701 88-89 0.21636165293038823 90-91 0.20416388909066716 92-93 0.20199432635237685 94-95 0.24175357238593403 96-97 0.26976503796252665 98-99 0.2876358065919994 100-101 0.2833609644558126 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3111226.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.83683002965441 #Duplication Level Percentage of deduplicated Percentage of total 1 84.5237935208227 62.409689756585905 2 9.814268602908145 14.493089653966052 3 2.7847189542055224 6.168444603060904 4 1.1046072122533872 3.26242779922735 5 0.5587539953420018 2.062831189122885 6 0.32737990010808604 1.4503616423643593 7 0.21261069598946586 1.09889498755825 8 0.13309090002792867 0.7861608131084719 9 0.08956159110662087 0.5951649581752476 >10 0.424253322436623 5.200501240639575 >50 0.0183507539715763 0.9354601253910837 >100 0.00809050692143967 1.1098085565607463 >500 3.309370315207154E-4 0.1745730600889932 >1k 1.8910687506788716E-4 0.25259161415014453 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.821250529534016E-5 0.0 22-23 0.0 0.0 0.0 2.249916913782541E-4 3.214167019689344E-5 24-25 0.0 0.0 0.0 3.3748753706738116E-4 6.428334039378688E-5 26-27 0.0 0.0 0.0 4.8212505295340167E-4 6.428334039378688E-5 28-29 0.0 3.214167019689344E-5 0.0 0.00200885438730584 6.428334039378688E-5 30-31 0.0 3.214167019689344E-5 0.0 0.0068301049168398575 6.428334039378688E-5 32-33 0.0 3.214167019689344E-5 0.0 0.017613635267897606 1.2856668078757376E-4 34-35 0.0 3.214167019689344E-5 0.0 0.03217381186709034 1.607083509844672E-4 36-37 0.0 3.214167019689344E-5 0.0 0.057115747939879655 1.607083509844672E-4 38-39 0.0 3.214167019689344E-5 0.0 0.09428758952258692 1.607083509844672E-4 40-41 3.214167019689344E-5 3.214167019689344E-5 0.0 0.13674673585268315 1.607083509844672E-4 42-43 3.214167019689344E-5 3.214167019689344E-5 0.0 0.18142365742636504 1.607083509844672E-4 44-45 3.214167019689344E-5 3.214167019689344E-5 0.0 0.2335895881559231 1.607083509844672E-4 46-47 3.214167019689344E-5 3.214167019689344E-5 0.0 0.29575157831671506 1.607083509844672E-4 48-49 3.214167019689344E-5 3.214167019689344E-5 0.0 0.3636187149374555 1.607083509844672E-4 50-51 3.214167019689344E-5 3.214167019689344E-5 0.0 0.439328419086238 1.607083509844672E-4 52-53 3.214167019689344E-5 3.214167019689344E-5 0.0 0.5170630484574248 1.607083509844672E-4 54-55 3.214167019689344E-5 3.214167019689344E-5 0.0 0.602222403644094 1.607083509844672E-4 56-57 3.214167019689344E-5 3.214167019689344E-5 0.0 0.7042561356841321 1.607083509844672E-4 58-59 3.214167019689344E-5 3.214167019689344E-5 0.0 0.8152734645442021 1.607083509844672E-4 60-61 3.214167019689344E-5 3.214167019689344E-5 0.0 0.9382474947175166 1.607083509844672E-4 62-63 3.214167019689344E-5 3.214167019689344E-5 0.0 1.064323196064831 1.607083509844672E-4 64-65 6.428334039378688E-5 3.214167019689344E-5 0.0 1.2002342484923951 1.607083509844672E-4 66-67 6.428334039378688E-5 3.214167019689344E-5 0.0 1.3559927822665405 1.607083509844672E-4 68-69 6.428334039378688E-5 3.214167019689344E-5 0.0 1.5304416972601798 1.607083509844672E-4 70-71 6.428334039378688E-5 3.214167019689344E-5 0.0 1.7234845684627218 1.607083509844672E-4 72-73 6.428334039378688E-5 3.214167019689344E-5 0.0 1.9220558069391296 1.9285002118136067E-4 74-75 6.428334039378688E-5 3.214167019689344E-5 0.0 2.1284856837786776 1.9285002118136067E-4 76-77 6.428334039378688E-5 3.214167019689344E-5 0.0 2.3504560581584233 2.089208562798074E-4 78-79 6.428334039378688E-5 3.214167019689344E-5 1.607083509844672E-5 2.610128611679126 2.2499169137825411E-4 80-81 6.428334039378688E-5 3.214167019689344E-5 3.214167019689344E-5 2.8975233557446485 2.2499169137825411E-4 82-83 6.428334039378688E-5 3.214167019689344E-5 3.214167019689344E-5 3.196874801123416 2.5713336157514753E-4 84-85 6.428334039378688E-5 3.214167019689344E-5 3.214167019689344E-5 3.4989743593040172 2.5713336157514753E-4 86-87 6.428334039378688E-5 3.214167019689344E-5 3.214167019689344E-5 3.817353673439345 2.5713336157514753E-4 88-89 6.428334039378688E-5 3.214167019689344E-5 3.214167019689344E-5 4.161896307114945 2.5713336157514753E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11375 0.0 29.520824 1 GTACATG 7850 0.0 29.166132 1 TACATGG 9405 0.0 26.570827 2 ACATGGG 9145 0.0 25.6216 3 TATCAAC 14225 0.0 22.54892 2 GGTATCA 8695 0.0 22.235659 1 ATCAACG 14875 0.0 21.722376 3 TCAACGC 14890 0.0 21.698397 4 CAACGCA 14775 0.0 21.692762 5 AACGCAG 15200 0.0 21.27255 6 CATGGGG 4400 0.0 20.504396 4 CATGGGA 5715 0.0 20.249584 4 ACGCAGA 15805 0.0 19.8908 7 GAGTACT 6075 0.0 18.919569 12-13 AGAGTAC 10745 0.0 18.779684 10-11 GTCTTAG 1200 0.0 18.655521 1 CGCAGAG 16605 0.0 18.647625 8 GAGTACA 6910 0.0 18.113111 1 AGTACAT 6680 0.0 17.325981 2 GCAGAGT 16800 0.0 17.082283 9 >>END_MODULE