FastQCFastQC Report
Fri 27 May 2016
SRR939620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3111226
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62170.19982476361408655No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47820.15370146688154443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47300.152030100031306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34110.10963523704160354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA130850.024.418141
GTACATG96300.024.1611881
TACATGG96700.022.8843862
ACATGGG95650.022.4925043
GGTATCA94650.022.0393771
TCAACGC149500.020.8601884
CAACGCA150150.020.7698825
AACGCAG151150.020.6802676
ATCAACG150900.020.6666553
TATCAAC157600.019.9692842
TAGGACG4100.019.5624394
AGAGTAC113950.019.2806810-11
CATGGGG46700.018.286044
ACGCAGA168650.018.2827767
CGCAGAG167850.018.1452068
GAGTACT64750.018.05812-13
AGTACTT72600.017.63156912-13
CATGGGA60700.017.1775634
GCAGAGT175150.016.7967479
GAGTACA79400.016.5836961
Produced by FastQC (version 0.11.5)