##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939620_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3111226 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46928734846006 31.0 31.0 34.0 30.0 34.0 2 31.679990460352286 31.0 31.0 34.0 30.0 34.0 3 31.740436728157967 31.0 31.0 34.0 30.0 34.0 4 35.54050846836585 37.0 35.0 37.0 33.0 37.0 5 35.29114728406101 37.0 35.0 37.0 33.0 37.0 6 35.28948330979492 37.0 35.0 37.0 32.0 37.0 7 35.19246399972229 37.0 35.0 37.0 32.0 37.0 8 35.147963857334695 37.0 35.0 37.0 32.0 37.0 9 36.75539128305048 39.0 35.0 39.0 32.0 39.0 10-11 36.629392561003286 39.0 35.0 39.0 32.0 39.0 12-13 36.56860382370165 38.5 35.0 39.0 32.0 39.0 14-15 37.66343782161759 39.0 36.0 41.0 32.0 41.0 16-17 37.60916805786529 39.0 36.0 41.0 32.0 41.0 18-19 37.55238867250402 39.0 36.0 41.0 32.0 41.0 20-21 37.48415126384261 39.0 36.0 41.0 32.0 41.0 22-23 37.35151673327492 39.0 36.0 41.0 31.5 41.0 24-25 37.22656052630057 39.0 36.0 40.5 31.0 41.0 26-27 36.960588205421274 39.0 36.0 40.0 31.0 41.0 28-29 36.76408592625543 39.0 36.0 40.0 30.0 41.0 30-31 36.53862769853428 38.0 35.0 40.0 30.0 41.0 32-33 36.4060039354261 38.0 35.0 40.0 30.0 41.0 34-35 36.3991553490489 38.0 35.0 40.0 30.0 41.0 36-37 36.35669170288497 38.0 35.0 40.0 30.0 41.0 38-39 36.432729734194815 38.0 35.0 40.0 30.0 41.0 40-41 36.33904174752975 38.0 35.0 40.0 30.0 41.0 42-43 36.18712237555228 38.0 35.0 40.0 30.0 41.0 44-45 36.016241668075544 38.0 35.0 40.0 29.0 41.0 46-47 35.78157324475946 38.0 35.0 40.0 28.5 41.0 48-49 35.54093209557904 38.0 34.0 40.0 27.5 41.0 50-51 35.32673245209445 38.0 34.0 40.0 27.0 41.0 52-53 35.111450759282675 38.0 34.0 40.0 27.0 41.0 54-55 34.83151770395336 38.0 33.5 40.0 26.0 41.0 56-57 34.02569774744747 37.5 32.5 40.0 24.0 41.0 58-59 30.82946851176996 35.5 27.5 39.0 8.5 40.5 60-61 30.565510509361907 34.5 26.5 39.0 11.0 40.0 62-63 32.42921102485001 35.5 30.5 39.0 21.0 40.0 64-65 32.4940340238864 36.0 31.0 39.0 20.0 40.0 66-67 27.498922450506647 32.0 19.0 37.0 5.0 39.5 68-69 19.832026024467524 20.0 8.0 32.0 2.0 37.0 70-71 17.87730544164905 15.5 7.5 29.0 2.0 35.0 72-73 19.593904621522192 20.0 7.5 31.0 2.0 35.5 74-75 21.083606430391107 23.5 9.5 32.5 2.0 36.0 76-77 23.00125818567986 27.5 12.5 33.0 2.0 35.5 78-79 25.256900013049517 30.5 16.5 34.5 2.0 36.5 80-81 26.878770458976625 32.0 23.5 35.0 2.0 37.0 82-83 28.02018175471663 33.0 27.0 35.0 2.0 36.0 84-85 28.07469772366263 34.0 28.0 35.0 2.0 36.0 86-87 27.803035041491682 34.0 27.0 35.0 2.0 36.0 88-89 27.368650011281726 33.0 26.5 35.0 2.0 35.0 90-91 25.52554105680526 32.5 16.5 34.5 2.0 35.0 92-93 23.872915853750257 31.5 4.5 34.0 2.0 35.0 94-95 19.996790172105786 28.0 2.0 34.0 2.0 35.0 96-97 17.26375486705241 18.5 2.0 33.0 2.0 35.0 98-99 17.5415869821093 21.5 2.0 33.0 2.0 35.0 100-101 16.768485478071987 18.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24034232672974198 1101 2 0.19803818651172378 1101 3 0.16336285086175906 1101 4 0.20626034650903335 1101 5 0.3555293098372445 1101 6 0.25012444958864677 1101 7 0.22678872561856167 1101 8 0.2868903095286157 1101 9 0.26033031963530107 1101 10-11 0.2786399072152932 1101 12-13 0.28544141657215505 1101 14-15 0.39454567176940003 1101 16-17 0.3915931638793282 1101 18-19 0.28615279199841126 1101 20-21 0.350620587530301 1101 22-23 0.38711643485171976 1101 24-25 0.26591224419224346 1101 26-27 0.29910302352244855 1101 28-29 0.389397008513491 1101 30-31 0.21497916181721877 1101 32-33 0.2768424803200631 1101 34-35 0.23598484025006172 1101 36-37 0.09574956053808847 1101 38-39 0.05961501298087768 1101 40-41 0.11205574199154 1101 42-43 0.2369463910287024 1101 44-45 0.22238763151305818 1101 46-47 0.22435880930221686 1101 48-49 0.14101775559226581 1101 50-51 0.11302431799013135 1101 52-53 0.1439629269372915 1101 54-55 0.15174596209004676 1101 56-57 -3.2610143552199418 1101 58-59 -10.510284084668964 1101 60-61 -11.783618137871851 1101 62-63 -4.106391610402838 1101 64-65 -1.226807192117377 1101 66-67 -2.3511023081057125 1101 68-69 -4.832957746770841 1101 70-71 -4.775091363948398 1101 72-73 -3.524344029180206 1101 74-75 -6.5314476996211095 1101 76-77 -5.1488945194953 1101 78-79 1.025458091239468 1101 80-81 1.8097502054578136 1101 82-83 0.436134865548226 1101 84-85 0.07887235271959625 1101 86-87 -0.027706666737959296 1101 88-89 -0.16845984157382432 1101 90-91 1.380923226488754 1101 92-93 0.9763528765922374 1101 94-95 -8.957130377437437 1101 96-97 -9.93487256943102 1101 98-99 -9.204614671607672 1101 100-101 -10.162706546849032 1102 1 0.2314561558292354 1102 2 0.31101043018935925 1102 3 0.29570293906756007 1102 4 0.23052193093658957 1102 5 0.2787395153937311 1102 6 0.3026908529660659 1102 7 0.36145425428635747 1102 8 0.399250007893599 1102 9 0.22709256949826795 1102 10-11 0.2547250568198649 1102 12-13 0.3288912187922364 1102 14-15 0.34288515867923053 1102 16-17 0.387452177939025 1102 18-19 0.40854269305673796 1102 20-21 0.3715862935205294 1102 22-23 0.34377785605482103 1102 24-25 0.22778352113331124 1102 26-27 0.24182563684107095 1102 28-29 0.19796575719917797 1102 30-31 0.24572268586515378 1102 32-33 0.08297315895168822 1102 34-35 0.15127096658861916 1102 36-37 5.513823523628503E-4 1102 38-39 0.10229981396443577 1102 40-41 0.042465668346387986 1102 42-43 0.1417204069871829 1102 44-45 0.0750547891594664 1102 46-47 0.005961230380300719 1102 48-49 -0.005379304045071365 1102 50-51 0.18059182180811462 1102 52-53 -0.08086338398860704 1102 54-55 -0.10360546310294438 1102 56-57 -3.693332634199855 1102 58-59 -10.251615280262705 1102 60-61 -8.015308730275382 1102 62-63 -2.553172608579537 1102 64-65 -0.7254198477462523 1102 66-67 -1.9582591362168955 1102 68-69 -5.1574796629562805 1102 70-71 -4.8059206642986325 1102 72-73 -3.4395271423074902 1102 74-75 -4.049641347765721 1102 76-77 -4.187544931212479 1102 78-79 1.8236926779501026 1102 80-81 2.187447804392683 1102 82-83 0.9021250833302226 1102 84-85 0.5264538692373613 1102 86-87 0.40570914974085426 1102 88-89 0.394344832132397 1102 90-91 1.8579752306060193 1102 92-93 1.277567860945659 1102 94-95 -8.630315544115625 1102 96-97 -9.701145889834859 1102 98-99 -8.698499651347046 1102 100-101 -9.62618031200141 1103 1 0.16343039633618517 1103 2 0.13574862422215972 1103 3 0.18709550297932154 1103 4 0.16819350791028143 1103 5 0.21024588239713893 1103 6 0.2840444034795837 1103 7 0.1756666082488607 1103 8 0.33787484866596174 1103 9 0.318120503876159 1103 10-11 0.3302183121544289 1103 12-13 0.2566049376631412 1103 14-15 0.3176903194063101 1103 16-17 0.31659430358066487 1103 18-19 0.40887542451311987 1103 20-21 0.30293019228187745 1103 22-23 0.2943813795924868 1103 24-25 0.4336860492968029 1103 26-27 0.4151999209297941 1103 28-29 0.3589002289838348 1103 30-31 0.4132493394152448 1103 32-33 0.35730091704058964 1103 34-35 0.2542953632752827 1103 36-37 0.33702311165206567 1103 38-39 0.29774543063257397 1103 40-41 0.37797248518101156 1103 42-43 0.29477565074384415 1103 44-45 0.29826609701918727 1103 46-47 0.21809737158525877 1103 48-49 0.17677588491905993 1103 50-51 0.13761174610754523 1103 52-53 0.07420506462623422 1103 54-55 0.10736029367149769 1103 56-57 0.5621471437152508 1103 58-59 -8.41510396301399 1103 60-61 -7.862788712085948 1103 62-63 -2.4335078729639754 1103 64-65 -0.5945074050787085 1103 66-67 -1.9536257696717243 1103 68-69 -3.165152192843429 1103 70-71 -3.7982089843822653 1103 72-73 -2.9772873892802387 1103 74-75 -3.6728113685476416 1103 76-77 -0.4593634089051939 1103 78-79 3.281797679904976 1103 80-81 2.676367295116492 1103 82-83 1.0517693698432744 1103 84-85 0.64728496042137 1103 86-87 0.6108228091948043 1103 88-89 0.45964340234180057 1103 90-91 1.8838868569910723 1103 92-93 1.3968184120239222 1103 94-95 -7.519586824565064 1103 96-97 -9.03319541751209 1103 98-99 -8.24242467037028 1103 100-101 -9.525672114843614 1104 1 0.3151948037584624 1104 2 0.2906228380586384 1104 3 0.3125220385072254 1104 4 0.24459772977566274 1104 5 0.33374882632863034 1104 6 0.28176536560992105 1104 7 0.2800068163312943 1104 8 0.2847899931862301 1104 9 0.355061129015553 1104 10-11 0.20630068090414966 1104 12-13 0.362820996028816 1104 14-15 0.47600225626789694 1104 16-17 0.44125244790021156 1104 18-19 0.5389161405352425 1104 20-21 0.4661033796452756 1104 22-23 0.433729560822691 1104 24-25 0.5398393818495535 1104 26-27 0.47247346680605773 1104 28-29 0.52467096195587 1104 30-31 0.3995960208015674 1104 32-33 0.5060820151047309 1104 34-35 0.3473034562990591 1104 36-37 0.39973080378820214 1104 38-39 0.3391507397453779 1104 40-41 0.2557731927281779 1104 42-43 0.30492503575209895 1104 44-45 0.30896537832075666 1104 46-47 0.0946592443271328 1104 48-49 0.029097018778735162 1104 50-51 0.07835118586846335 1104 52-53 0.16416991029286265 1104 54-55 0.16639295012361544 1104 56-57 0.6037423726505295 1104 58-59 -8.284086466693903 1104 60-61 -7.984049905158788 1104 62-63 -2.4093925857076286 1104 64-65 -0.5425442962201537 1104 66-67 -1.7827512463879032 1104 68-69 -3.179394437145138 1104 70-71 -3.8935759164488157 1104 72-73 -2.9974885858099682 1104 74-75 -3.5580886445189144 1104 76-77 -0.07651919752199277 1104 78-79 3.69442062249831 1104 80-81 3.0809048804235957 1104 82-83 1.5388624884034208 1104 84-85 1.049863757981477 1104 86-87 0.975015848581041 1104 88-89 0.7999894815603206 1104 90-91 2.285166533270118 1104 92-93 2.124259770358371 1104 94-95 -6.046332414243462 1104 96-97 -7.737932470904301 1104 98-99 -5.697543305791129 1104 100-101 -5.0025318655015365 1105 1 0.19481316473341792 1105 2 0.21641291840958843 1105 3 0.21714585506533624 1105 4 0.15833446851198119 1105 5 0.13406469060490878 1105 6 0.21052594104804712 1105 7 0.23308565370455625 1105 8 0.1694572166021544 1105 9 0.18942717626369898 1105 10-11 0.15532000485947606 1105 12-13 0.17210692314222342 1105 14-15 0.26801916437909057 1105 16-17 0.33675722223491533 1105 18-19 0.24379619464342994 1105 20-21 0.18394289177625467 1105 22-23 0.35932694717869396 1105 24-25 0.44114211215909194 1105 26-27 0.3046845587060574 1105 28-29 0.41504154293350837 1105 30-31 0.30299373001619045 1105 32-33 0.2320048829509247 1105 34-35 0.22724163335091419 1105 36-37 0.2706510067918444 1105 38-39 0.3364701599427846 1105 40-41 0.2689867200564535 1105 42-43 0.22452355379189015 1105 44-45 0.30629161799711113 1105 46-47 0.1848242967007252 1105 48-49 0.09711694984255104 1105 50-51 0.23118165432120463 1105 52-53 0.2806875276298584 1105 54-55 0.112443877292975 1105 56-57 0.7232128243728155 1105 58-59 -8.70598979376031 1105 60-61 -7.8111941241990515 1105 62-63 -2.3116806432962065 1105 64-65 -0.4972767698095346 1105 66-67 -1.5896458095495696 1105 68-69 -2.950601617811671 1105 70-71 -3.642818429710079 1105 72-73 -2.5885615031760665 1105 74-75 -3.432363331732148 1105 76-77 -0.024357877326824706 1105 78-79 3.826526169847181 1105 80-81 3.2700691830042246 1105 82-83 1.7276057318735063 1105 84-85 1.3495919230590303 1105 86-87 1.2821560878433331 1105 88-89 1.2336111214806387 1105 90-91 2.4570745815142594 1105 92-93 2.1381524478466787 1105 94-95 -6.370096444884254 1105 96-97 -7.631889922951125 1105 98-99 -5.514475792250092 1105 100-101 -5.191519561063211 1106 1 0.060830760854539534 1106 2 0.08901505476131888 1106 3 0.1396407816851415 1106 4 0.09767448792317168 1106 5 0.13614936318456472 1106 6 0.14019397602181272 1106 7 0.1896751657777287 1106 8 0.1967792421448209 1106 9 0.05591604703921149 1106 10-11 0.162297915873296 1106 12-13 0.2089787378367447 1106 14-15 0.1712903916959405 1106 16-17 0.26469649152811314 1106 18-19 0.18250265425736956 1106 20-21 0.09993188229613992 1106 22-23 0.1637245069143347 1106 24-25 0.050063278343280615 1106 26-27 0.09152398639795933 1106 28-29 0.24449561361210215 1106 30-31 0.2862109603217391 1106 32-33 0.22870963673268108 1106 34-35 0.19208808044420778 1106 36-37 0.1372303861098274 1106 38-39 0.2145026337775917 1106 40-41 0.08871628683390753 1106 42-43 0.20990651171609898 1106 44-45 0.14925369474276096 1106 46-47 0.08613129607469716 1106 48-49 0.08484455169179483 1106 50-51 0.01290516105279238 1106 52-53 0.07836102042629278 1106 54-55 0.05411926219061769 1106 56-57 0.7701741532412925 1106 58-59 -8.7017168101926 1106 60-61 -7.888219344745789 1106 62-63 -2.206855119957254 1106 64-65 -0.3612194969842264 1106 66-67 0.4838161404491004 1106 68-69 -2.393798841702843 1106 70-71 -2.3122267874474574 1106 72-73 -1.0828839740837886 1106 74-75 -1.0317045976052803 1106 76-77 2.1547315682216954 1106 78-79 4.472783072655361 1106 80-81 3.5520596636763635 1106 82-83 1.9003112615428037 1106 84-85 1.3741957162247793 1106 86-87 1.2015611193480105 1106 88-89 1.0667056663189562 1106 90-91 2.378447167194512 1106 92-93 1.9082530471287704 1106 94-95 -6.584775880082939 1106 96-97 -7.855726126648214 1106 98-99 -5.846285711005983 1106 100-101 -5.336402379635773 1107 1 -0.0027126380915802883 1107 2 0.008769210128733107 1107 3 0.0015570181155233342 1107 4 0.013999255791887322 1107 5 0.025019049965074203 1107 6 -0.0020420000513539094 1107 7 0.049525286505627264 1107 8 -0.003536178776300858 1107 9 -0.003911919207460812 1107 10-11 0.06601320835451929 1107 12-13 -0.028154486313312077 1107 14-15 -0.04691424700373403 1107 16-17 0.05822706751658302 1107 18-19 0.0737518444970604 1107 20-21 -0.028116987965532303 1107 22-23 0.13008860599814653 1107 24-25 0.04171924168462482 1107 26-27 0.0882866978821113 1107 28-29 0.04399861007579631 1107 30-31 -0.022432337106373268 1107 32-33 0.03386535609428876 1107 34-35 0.10659141343167278 1107 36-37 0.13514989803818622 1107 38-39 0.1750038578827855 1107 40-41 -0.029836303417560828 1107 42-43 -0.14223546205187887 1107 44-45 -0.3383383935835198 1107 46-47 -0.1940450704221135 1107 48-49 -0.3161417433778482 1107 50-51 -0.31353919193819735 1107 52-53 -0.1301659309207892 1107 54-55 -0.23645726611803752 1107 56-57 0.370881694458447 1107 58-59 -8.793168896219292 1107 60-61 -8.148229349316903 1107 62-63 -2.6119811831620297 1107 64-65 -0.8247680138959481 1107 66-67 0.3689060694145425 1107 68-69 -2.749142874154952 1107 70-71 -2.7250292732363377 1107 72-73 -1.2629035064840117 1107 74-75 -1.0824844563774754 1107 76-77 2.11212853586213 1107 78-79 4.172612987935501 1107 80-81 3.063723892882699 1107 82-83 1.4676126844774622 1107 84-85 1.051057358685103 1107 86-87 0.9439031004073293 1107 88-89 0.7340175705667278 1107 90-91 2.115988601683153 1107 92-93 1.685131305450021 1107 94-95 -6.647573142742543 1107 96-97 -8.314538597377657 1107 98-99 -6.204264093560592 1107 100-101 -5.424717082639528 1108 1 -0.02448897612680412 1108 2 -0.08159173898814842 1108 3 -0.008371535124105378 1108 4 -0.024802478578394016 1108 5 0.019474777185806147 1108 6 0.06004341879823727 1108 7 -0.002582528596917655 1108 8 0.09615271577136753 1108 9 -0.055229012300451075 1108 10-11 -0.08669945864332362 1108 12-13 -0.027133122556143974 1108 14-15 -0.13149602011348094 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0.9364695265016785 2315 84-85 0.7914754380308544 2315 86-87 0.7428821932321092 2315 88-89 0.7954926356521455 2315 90-91 2.422327942645193 2315 92-93 4.128322893249035 2315 94-95 6.879778950113604 2315 96-97 4.492777737707446 2315 98-99 6.775119049245223 2315 100-101 7.00443106117857 2316 1 -0.395008658647793 2316 2 -0.3741909187638015 2316 3 -0.5372563228312224 2316 4 -0.3901898610693664 2316 5 -0.5463549530564364 2316 6 -0.624205864140265 2316 7 -0.44117861699875505 2316 8 -0.43939376099713456 2316 9 -0.6574651348395832 2316 10-11 -0.6452832525041998 2316 12-13 -0.5194601548246851 2316 14-15 -0.6135741198928528 2316 16-17 -0.7782641249730133 2316 18-19 -0.7279541268956962 2316 20-21 -0.6104702131631043 2316 22-23 -0.8566193515020686 2316 24-25 -0.6332116461565249 2316 26-27 -0.7637546847115573 2316 28-29 -0.752306992718772 2316 30-31 -0.7058953181076575 2316 32-33 -0.64693280901988 2316 34-35 -0.7177816483793507 2316 36-37 -0.48640097442523 2316 38-39 -0.6072281289621699 2316 40-41 -0.7150954521076471 2316 42-43 -0.7477389329937338 2316 44-45 -0.6565927657581483 2316 46-47 -0.6938885366761838 2316 48-49 -0.6796489718597982 2316 50-51 -0.5085015161366542 2316 52-53 -0.5441110705434085 2316 54-55 -0.40275648160558575 2316 56-57 0.30867253689659435 2316 58-59 3.494426219708778 2316 60-61 3.521381809905389 2316 62-63 0.8394833611686963 2316 64-65 0.35590716041784276 2316 66-67 -1.775095803879104 2316 68-69 -3.325981821649428 2316 70-71 -3.076567922376581 2316 72-73 -0.3953844504400017 2316 74-75 5.999903361408851 2316 76-77 6.179389626118304 2316 78-79 5.111134838403515 2316 80-81 3.3902627336969147 2316 82-83 1.6078598471549945 2316 84-85 1.3014621102281687 2316 86-87 1.0211976386848072 2316 88-89 1.1386911662171535 2316 90-91 2.5708901341987236 2316 92-93 4.266553177677594 2316 94-95 6.987116843770913 2316 96-97 3.818261666292713 2316 98-99 6.355211199846625 2316 100-101 6.37778561357438 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 24.0 4 41.0 5 102.0 6 221.0 7 583.0 8 1300.0 9 2219.0 10 3665.0 11 5852.0 12 8570.0 13 11607.0 14 14772.0 15 19964.0 16 26724.0 17 35378.0 18 40072.0 19 42974.0 20 46028.0 21 47783.0 22 47861.0 23 49107.0 24 52112.0 25 55300.0 26 69010.0 27 97843.0 28 138912.0 29 184023.0 30 223228.0 31 249247.0 32 258742.0 33 258393.0 34 263443.0 35 279243.0 36 279115.0 37 211344.0 38 81084.0 39 5333.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.176953418742 19.70758637717866 13.936762347140629 24.178697856938708 2 15.66874509177398 21.028760958825067 39.22990154777683 24.072592401624124 3 19.150673193158816 24.84693288831991 30.02789583625957 25.9744980822617 4 12.903112792191887 17.121160597140804 37.38291593089026 32.59281067977704 5 13.461016456373628 37.88529423872548 34.895436675380274 13.758252629520614 6 32.069608572312006 37.07811647241313 17.313753484960593 13.538521470314274 7 28.293830149272342 32.39716433328855 21.416926960625812 17.892078556813296 8 25.762321042985683 36.03995471875681 19.848792532323312 18.348931705934195 9 26.081390422939382 16.385952033057066 20.15726276393936 37.37539478006419 10-11 24.726602953305225 26.55909599624071 28.16675805614893 20.54754299430514 12-13 25.712162986552567 23.785655558291168 28.21484199476348 22.287339460392783 14-15 22.4906941320882 25.254410745147627 26.167062386358488 26.087832736405687 16-17 22.446392515362113 28.462911405343107 26.700792549303713 22.38990352999107 18-19 22.349414464806095 27.5492986900449 28.272183827950094 21.829103017198918 20-21 23.020121328376657 26.802922706354344 28.13016476462976 22.046791200639234 22-23 22.692581638235215 26.722375680840926 28.027102499143425 22.55794018178043 24-25 22.725415082146995 26.825299966588684 28.012807831264986 22.43647711999933 26-27 22.658749520284953 26.949394803713783 28.04684140186718 22.345014274134087 28-29 22.45167886698034 27.199347002377383 28.040857671685487 22.30811645895679 30-31 22.67458894604166 26.848009077299277 27.98529117510686 22.492110801552208 32-33 22.462248635627915 26.95821165000266 28.026405262666437 22.553134451702988 34-35 22.94354602178446 26.8926647193774 27.859521963253286 22.30426729558485 36-37 22.745206845525317 26.733320465804546 27.93450427845332 22.58696841021682 38-39 22.605916733412464 27.06260594890006 28.08635780250321 22.245119515184268 40-41 22.775375595338335 26.832964923632797 28.05771607216873 22.333943408860137 42-43 22.54363180840222 26.93156725790346 27.812583641304904 22.712217292389415 44-45 22.82203416003202 26.907362821336058 27.640804290786207 22.629798727845714 46-47 22.77037272228798 27.107971837252094 27.420253238260816 22.701402202199112 48-49 22.81711651233997 27.135229685536373 27.46110256391287 22.586551238210788 50-51 22.486247864106947 27.184625590511608 27.643043521962007 22.68608302341944 52-53 22.89651397414152 27.211594186119058 27.241648351153575 22.65024348858585 54-55 22.626717929885242 26.91696824969081 27.505738491984122 22.950575328439832 56-57 22.618743781232546 27.175074979016813 27.560146045245848 22.646035194504794 58-59 21.809182883711113 26.735803701845644 29.062193383287223 22.39282003115602 60-61 22.08689558152758 26.939110590652977 28.553833392406265 22.420160435413184 62-63 22.7545843659785 27.060684580867267 27.558572094610202 22.626158958544035 64-65 22.5307392000576 27.048969295834947 27.539973656246808 22.880317847860645 66-67 22.30774343657363 26.896848056978122 28.243929067265316 22.551479439182927 68-69 21.574505873997254 26.239015169101123 29.94484082963976 22.241638127261872 70-71 22.398868164053017 26.287291415665504 28.947968229732286 22.365872190549197 72-73 21.81797498086941 26.35859117005561 29.244769266653847 22.578664582421133 74-75 21.941986664656522 26.66956357797744 28.6755796888854 22.71287006848064 76-77 20.942297155336675 26.96353465706917 28.884663610563116 23.209504577031037 78-79 22.136048928817438 26.882937461634963 27.940269010229706 23.04074459931789 80-81 22.208801197956372 27.0978304243491 27.477111464027065 23.21625691366746 82-83 22.57936830628984 26.991314990921577 27.250586023566935 23.178730679221644 84-85 22.45563137710312 26.949393308872082 27.14420688359511 23.45076843042968 86-87 22.34847644770494 26.934850826839824 27.37339783612857 23.343274889326665 88-89 22.462258039476726 26.85694109925494 27.129406081301404 23.55139477996693 90-91 22.213267634826337 26.82509513980363 27.619785209225334 23.341852016144703 92-93 22.105718140809476 26.891999607001736 27.59362371574069 23.408658536448097 94-95 21.0203174263187 26.778809253751202 28.41932662522372 23.78154669470637 96-97 20.68396176693664 26.742454686609584 28.62511617508556 23.948467371368213 98-99 21.397981231881406 26.553617118604972 27.92219451177949 24.12620713773413 100-101 20.624619444454773 26.64402016008912 28.490729840678657 24.24063055477745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 201.0 1 164.0 2 146.0 3 189.5 4 224.0 5 232.0 6 286.0 7 472.5 8 697.0 9 868.5 10 1003.0 11 1131.5 12 1260.0 13 1430.5 14 1604.5 15 1760.5 16 1999.0 17 2273.0 18 2487.0 19 2760.0 20 3261.5 21 3992.5 22 4904.0 23 6124.0 24 7714.0 25 9885.5 26 13143.0 27 17201.5 28 21780.5 29 26916.5 30 32732.0 31 39188.5 32 46298.0 33 53616.0 34 61478.0 35 69646.5 36 77514.0 37 84773.0 38 89845.0 39 93487.0 40 97443.0 41 101780.5 42 106278.0 43 111421.0 44 116210.5 45 120273.0 46 124757.5 47 129970.5 48 134539.5 49 134606.5 50 130172.0 51 124726.0 52 119073.5 53 113325.5 54 106254.5 55 97271.5 56 87688.5 57 77114.0 58 66766.0 59 56320.0 60 45370.0 61 35375.0 62 26726.5 63 19847.5 64 14281.5 65 9974.5 66 6833.5 67 4610.0 68 2990.5 69 1802.0 70 1100.0 71 697.5 72 433.5 73 264.5 74 151.5 75 89.5 76 45.5 77 15.5 78 9.0 79 7.0 80 7.0 81 5.5 82 1.5 83 1.5 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024716944381411057 2 0.012117409664228827 3 2.8927503177204097E-4 4 0.0 5 0.06151915675685405 6 0.0 7 0.0 8 1.2856668078757376E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 5.464083933471886E-4 16-17 0.0 18-19 4.0177087746116804E-4 20-21 0.0 22-23 0.0 24-25 1.4463751588602048E-4 26-27 1.2856668078757376E-4 28-29 0.0011410292919897171 30-31 0.0054158714281765455 32-33 0.005769429800342373 34-35 0.0020409960575027337 36-37 0.08440402593704219 38-39 0.07765427519569455 40-41 0.08339156332584004 42-43 0.0872324929143688 44-45 0.08993239321090786 46-47 0.08522363852706297 48-49 0.07537221661171513 50-51 0.06953850347097897 52-53 0.05914067316228394 54-55 0.048983905380065605 56-57 0.0703741868960982 58-59 0.7256785588703617 60-61 0.33874106220506 62-63 0.0026195461210468153 64-65 0.001671366850238459 66-67 1.1100607927550104 68-69 2.3848155035989027 70-71 2.3455865951235944 72-73 1.8180453621819823 74-75 1.290134500033106 76-77 0.7456224652275342 78-79 0.5653398370931588 80-81 0.04059492945867642 82-83 9.642501059068034E-5 84-85 0.0014945876641555451 86-87 0.0013820918184664181 88-89 1.607083509844672E-5 90-91 0.3340965908616089 92-93 0.05777465217891596 94-95 0.960425247153373 96-97 1.6626082451098054 98-99 0.6471082460740557 100-101 0.5749019839767346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3111226.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.496556736170966 #Duplication Level Percentage of deduplicated Percentage of total 1 73.12761273763545 38.389478710620175 2 14.341951657239136 15.058061577633508 3 5.2406739144690535 8.253520064600876 4 2.4284706345600644 5.099453857972301 5 1.3741120241128437 3.606807491784731 6 0.8215244727461448 2.5876323656202556 7 0.5507645846063434 2.0239270984842377 8 0.3873927068539629 1.6269426571630323 9 0.2841630574728146 1.342582386205088 >10 1.3108224854276544 11.946461534966048 >50 0.08190917833928509 2.9634974701137455 >100 0.046615677145378585 4.6725347870057385 >500 0.0026988038963326206 1.0510569087663684 >1k 0.0012267290433533957 1.1778586395766348 >5k 6.133645216766979E-5 0.20018444948736847 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6217 0.19982476361408655 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4782 0.15370146688154443 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4730 0.152030100031306 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3411 0.10963523704160354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 4.821250529534016E-5 16-17 0.0 0.0 0.0 0.0 6.428334039378688E-5 18-19 0.0 0.0 0.0 0.0 9.642501059068034E-5 20-21 0.0 0.0 0.0 3.214167019689344E-5 9.642501059068034E-5 22-23 0.0 0.0 0.0 1.6070835098446723E-4 9.642501059068034E-5 24-25 0.0 0.0 0.0 2.8927503177204097E-4 9.642501059068034E-5 26-27 0.0 0.0 0.0 4.499833827565082E-4 9.642501059068034E-5 28-29 0.0 0.0 0.0 0.0019445710469120532 9.642501059068034E-5 30-31 0.0 0.0 0.0 0.007103309113513451 9.642501059068034E-5 32-33 0.0 0.0 0.0 0.01877073539498577 1.607083509844672E-4 34-35 0.0 0.0 0.0 0.034295162100085305 1.607083509844672E-4 36-37 0.0 0.0 0.0 0.06050669414565191 1.607083509844672E-4 38-39 0.0 0.0 0.0 0.10015344433351997 1.607083509844672E-4 40-41 0.0 0.0 0.0 0.14476608256680806 1.607083509844672E-4 42-43 0.0 0.0 0.0 0.19164470854897717 1.607083509844672E-4 44-45 0.0 0.0 0.0 0.24575521032544728 1.607083509844672E-4 46-47 0.0 0.0 0.0 0.3103278257510062 1.9285002118136067E-4 48-49 0.0 0.0 0.0 0.3766521622022958 2.2499169137825411E-4 50-51 0.0 0.0 0.0 0.4469942074281971 2.2499169137825411E-4 52-53 0.0 0.0 0.0 0.5159220191654351 2.5713336157514753E-4 54-55 0.0 0.0 0.0 0.5945405444670364 2.8927503177204097E-4 56-57 0.0 1.607083509844672E-5 0.0 0.6716966237746792 2.8927503177204097E-4 58-59 0.0 6.428334039378688E-5 0.0 0.7280731133000302 2.8927503177204097E-4 60-61 0.0 6.428334039378688E-5 0.0 0.7756909976967279 2.8927503177204097E-4 62-63 4.821250529534016E-5 6.428334039378688E-5 0.0 0.8183269232129071 2.8927503177204097E-4 64-65 6.428334039378688E-5 1.2856668078757376E-4 0.0 0.8676483161300401 2.8927503177204097E-4 66-67 6.428334039378688E-5 1.2856668078757376E-4 0.0 0.910525304172696 2.8927503177204097E-4 68-69 6.428334039378688E-5 1.2856668078757376E-4 0.0 0.9615341347751658 2.8927503177204097E-4 70-71 6.428334039378688E-5 1.2856668078757376E-4 0.0 1.0395580391781247 2.8927503177204097E-4 72-73 6.428334039378688E-5 1.2856668078757376E-4 0.0 1.1442756006796033 2.8927503177204097E-4 74-75 6.428334039378688E-5 1.2856668078757376E-4 0.0 1.2889773999060177 2.8927503177204097E-4 76-77 6.428334039378688E-5 1.2856668078757376E-4 0.0 1.474691970303668 2.8927503177204097E-4 78-79 6.428334039378688E-5 1.2856668078757376E-4 0.0 1.7134402965261928 2.8927503177204097E-4 80-81 6.428334039378688E-5 1.2856668078757376E-4 0.0 2.0003689863738603 2.8927503177204097E-4 82-83 6.428334039378688E-5 1.2856668078757376E-4 0.0 2.289724372321394 2.8927503177204097E-4 84-85 6.428334039378688E-5 1.2856668078757376E-4 0.0 2.5483362507255984 2.8927503177204097E-4 86-87 6.428334039378688E-5 1.2856668078757376E-4 0.0 2.7873899228149934 2.8927503177204097E-4 88-89 6.428334039378688E-5 1.2856668078757376E-4 0.0 3.0207384484444395 2.8927503177204097E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13085 0.0 24.41814 1 GTACATG 9630 0.0 24.161188 1 TACATGG 9670 0.0 22.884386 2 ACATGGG 9565 0.0 22.492504 3 GGTATCA 9465 0.0 22.039377 1 TCAACGC 14950 0.0 20.860188 4 CAACGCA 15015 0.0 20.769882 5 AACGCAG 15115 0.0 20.680267 6 ATCAACG 15090 0.0 20.666655 3 TATCAAC 15760 0.0 19.969284 2 TAGGACG 410 0.0 19.562439 4 AGAGTAC 11395 0.0 19.28068 10-11 CATGGGG 4670 0.0 18.28604 4 ACGCAGA 16865 0.0 18.282776 7 CGCAGAG 16785 0.0 18.145206 8 GAGTACT 6475 0.0 18.058 12-13 AGTACTT 7260 0.0 17.631569 12-13 CATGGGA 6070 0.0 17.177563 4 GCAGAGT 17515 0.0 16.796747 9 GAGTACA 7940 0.0 16.583696 1 >>END_MODULE