FastQCFastQC Report
Fri 27 May 2016
SRR939619_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939619_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113378
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55810.2640795920086232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39320.1860528499870823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26530.1255336243681916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49250.036.228781
GTATCAA73500.032.0878871
ATCAACG87750.026.7682443
TATCAAC88950.026.3543952
TCAACGC89650.026.253244
CAACGCA91200.025.859085
AACGCAG93350.025.2096866
GTACATG76350.024.177571
TACATGG73750.024.0004222
ACATGGG75600.022.7221053
ACGCAGA105750.021.9401137
CGCAGAG105700.021.7719678
CATGGGG31650.020.0901224
GTGTAGC16250.019.857641
GAGTACT55500.019.15257312-13
GCAGAGT110400.019.1272569
CATGGGA50600.017.91164
GTACTTT61050.017.6847414-15
AGAGTAC95150.017.3307810-11
AGTACAT54250.017.3195882
Produced by FastQC (version 0.11.5)