##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939619_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2113378 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.004562364139307 31.0 30.0 33.0 26.0 34.0 2 30.14665194773486 31.0 30.0 34.0 25.0 34.0 3 30.190912368729116 31.0 30.0 34.0 26.0 34.0 4 33.76486269848555 35.0 35.0 37.0 28.0 37.0 5 33.749045840356054 35.0 33.0 37.0 28.0 37.0 6 33.8357335980596 35.0 35.0 37.0 28.0 37.0 7 33.63620847761262 35.0 33.0 37.0 28.0 37.0 8 33.6280130672317 35.0 33.0 37.0 28.0 37.0 9 34.883586845325354 37.0 34.0 39.0 28.0 39.0 10-11 34.75720694546834 37.0 34.0 39.0 27.0 39.0 12-13 34.594108105601556 37.0 34.0 39.0 27.0 39.0 14-15 35.27347332091088 38.0 33.5 40.0 27.0 41.0 16-17 34.995735500227596 38.0 33.0 40.0 25.5 41.0 18-19 34.93331907495961 38.0 33.0 40.0 25.5 41.0 20-21 35.05618020060774 38.0 34.0 40.0 25.5 41.0 22-23 34.90635158499805 38.0 33.0 40.0 25.0 41.0 24-25 34.78046710053762 38.0 33.0 40.0 25.0 41.0 26-27 34.912324250560005 38.0 34.0 40.0 25.0 41.0 28-29 34.78694701089914 38.0 33.5 40.0 24.0 41.0 30-31 34.567658033726104 38.0 33.0 40.0 24.0 41.0 32-33 34.340581997162836 38.0 33.0 40.0 24.0 41.0 34-35 34.115298824914426 38.0 33.0 40.0 23.5 41.0 36-37 33.83413118713264 38.0 32.5 40.0 21.5 41.0 38-39 33.65955025556242 38.0 32.0 40.0 20.0 41.0 40-41 33.3756112252517 37.5 31.5 40.0 18.5 41.0 42-43 33.058782432674136 37.0 31.0 40.0 17.0 41.0 44-45 32.80248824393932 37.0 31.0 40.0 15.5 41.0 46-47 32.37299905648682 37.0 30.5 40.0 13.5 41.0 48-49 32.014004120417646 37.0 30.0 39.5 9.0 41.0 50-51 31.420550417388654 35.5 30.0 38.5 9.0 40.0 52-53 31.49402851737834 36.0 30.0 39.0 8.5 40.0 54-55 31.522736112517496 36.0 30.0 39.0 8.0 40.0 56-57 31.372398595991818 36.0 29.5 39.0 7.0 40.0 58-59 30.970593523733093 36.0 28.5 39.0 2.0 40.0 60-61 30.552508827100503 35.0 28.0 39.0 2.0 40.0 62-63 30.12034122622645 35.0 28.0 38.0 2.0 40.0 64-65 29.49267215803325 34.0 27.0 38.0 2.0 40.0 66-67 28.941703992376183 34.0 26.0 38.0 2.0 40.0 68-69 28.407039583075058 34.0 26.0 37.0 2.0 39.0 70-71 27.837081913410664 33.0 25.5 36.5 2.0 39.0 72-73 27.08753734542519 32.5 23.5 36.0 2.0 38.5 74-75 26.60444818674179 32.0 22.0 35.0 2.0 38.0 76-77 26.201182419803747 32.0 21.5 35.0 2.0 37.5 78-79 25.59800471094144 31.0 20.0 35.0 2.0 37.0 80-81 24.91124375289229 31.0 18.0 34.5 2.0 36.0 82-83 24.22417120836878 30.5 13.0 34.0 2.0 36.0 84-85 23.537759927471562 30.0 7.0 34.0 2.0 35.0 86-87 22.847627352986546 29.5 2.0 34.0 2.0 35.0 88-89 22.01821893669755 29.0 2.0 34.0 2.0 35.0 90-91 20.750271366504244 27.5 2.0 33.5 2.0 35.0 92-93 19.09650947440543 25.0 2.0 32.0 2.0 34.0 94-95 18.808071248967295 25.0 2.0 32.0 2.0 34.0 96-97 18.16737777151082 24.5 2.0 32.0 2.0 34.0 98-99 17.164832083990653 21.5 2.0 32.0 2.0 34.0 100-101 15.573436223903155 8.5 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1355736415111046 1101 2 1.0619226873569367 1101 3 1.163422517064749 1101 4 1.2267757443837368 1101 5 0.9332762206564382 1101 6 0.8781765178515428 1101 7 0.9247523195734786 1101 8 0.9515921936285849 1101 9 1.322410457547221 1101 10-11 1.1428465578231268 1101 12-13 1.0205789354715193 1101 14-15 1.3248202426697802 1101 16-17 1.1528298631604983 1101 18-19 1.1706278048580359 1101 20-21 1.4871978658345029 1101 22-23 1.415362446050807 1101 24-25 1.3653624334177792 1101 26-27 1.4377854894735265 1101 28-29 1.4463805078423277 1101 30-31 1.513618902258223 1101 32-33 1.1604206551471066 1101 34-35 1.2121215062281294 1101 36-37 1.2032914174096305 1101 38-39 0.9698675026136314 1101 40-41 1.1497071869902058 1101 42-43 1.3288914702409542 1101 44-45 1.232886882069245 1101 46-47 1.0736465873729983 1101 48-49 0.810133587078429 1101 50-51 1.1971503201407643 1101 52-53 1.3769184137651393 1101 54-55 1.415002619413233 1101 56-57 1.3582034830220842 1101 58-59 1.2230041461028733 1101 60-61 0.8553050169554091 1101 62-63 1.0008514276288771 1101 64-65 0.9669637287109261 1101 66-67 0.9186093044525698 1101 68-69 0.9004228200313484 1101 70-71 0.8133163066353681 1101 72-73 0.9538585426340589 1101 74-75 0.9724221492403977 1101 76-77 0.3831888256353899 1101 78-79 0.6814955011618018 1101 80-81 0.519835517874494 1101 82-83 0.4479065705968601 1101 84-85 -0.12154469083022335 1101 86-87 -0.5933926136389438 1101 88-89 -1.1358078690708417 1101 90-91 -4.3716205305197455 1101 92-93 -9.25762710397706 1101 94-95 -7.549354290726605 1101 96-97 -4.4300089112617105 1101 98-99 -3.228423621695182 1101 100-101 -2.5853246308541475 1102 1 0.9858075993609567 1102 2 1.012623499605759 1102 3 1.028540628043789 1102 4 1.0759168572872682 1102 5 0.8947176911003965 1102 6 0.9882109983330452 1102 7 0.899719478211999 1102 8 0.8635782292694216 1102 9 0.9691438328608939 1102 10-11 1.0580738353744934 1102 12-13 0.9646486817970583 1102 14-15 1.2576540571261035 1102 16-17 1.2851096491551033 1102 18-19 1.1901249310915034 1102 20-21 1.2048730040394418 1102 22-23 1.4776072916669278 1102 24-25 1.3042463990504984 1102 26-27 1.2336075907074644 1102 28-29 1.0485271654525619 1102 30-31 1.1242723274294448 1102 32-33 1.0237482633526156 1102 34-35 0.9985620038130847 1102 36-37 1.0736410039740392 1102 38-39 0.9577865100380194 1102 40-41 1.0128188499776982 1102 42-43 0.9619791714164094 1102 44-45 0.7821626327626277 1102 46-47 0.8424655129593006 1102 48-49 0.8727186385397196 1102 50-51 0.7790071188332348 1102 52-53 0.9338022166650859 1102 54-55 0.929343592932593 1102 56-57 0.8552313987689608 1102 58-59 1.0632860511612705 1102 60-61 0.7935847781863288 1102 62-63 0.7633439690431878 1102 64-65 0.5719701495803413 1102 66-67 0.9045750763248002 1102 68-69 0.7256404456421883 1102 70-71 0.6135999982166602 1102 72-73 0.6353338921557672 1102 74-75 0.4550225659778171 1102 76-77 0.22700781018285454 1102 78-79 0.17396734131576252 1102 80-81 -0.17878529234606688 1102 82-83 -0.3349658928943988 1102 84-85 -0.6337957863284203 1102 86-87 -0.9109587031938595 1102 88-89 -1.2611452742613096 1102 90-91 -5.298857310250316 1102 92-93 -10.51572350192238 1102 94-95 -9.215907162370534 1102 96-97 -6.0605127873678235 1102 98-99 -4.74849974643811 1102 100-101 -4.00186099337561 1103 1 0.8667568381305415 1103 2 0.9445242906380678 1103 3 0.821258708961345 1103 4 0.8561746693153793 1103 5 0.8772087647777411 1103 6 0.5840831795467949 1103 7 0.9796779037146663 1103 8 0.7637791498801363 1103 9 0.8779372721134635 1103 10-11 0.8151654895904628 1103 12-13 0.7488561850365159 1103 14-15 1.017083479060524 1103 16-17 1.0130931150869245 1103 18-19 0.8518688718478415 1103 20-21 0.9036283903187154 1103 22-23 0.949680352463929 1103 24-25 0.6534586147501145 1103 26-27 0.7458473369753875 1103 28-29 0.9353253251428484 1103 30-31 1.0236009585594914 1103 32-33 0.9745080122189478 1103 34-35 0.8188744735410793 1103 36-37 0.8797521587718009 1103 38-39 0.9364856203118705 1103 40-41 1.0269676269603778 1103 42-43 1.0641931729503753 1103 44-45 0.8787819696904293 1103 46-47 0.8177003097195055 1103 48-49 0.7774042029123827 1103 50-51 0.6840525852414316 1103 52-53 0.9392851953957617 1103 54-55 1.034419488043607 1103 56-57 0.9188357071085882 1103 58-59 0.7384699054864186 1103 60-61 0.6814880582181466 1103 62-63 0.58992247224732 1103 64-65 0.4768455799413509 1103 66-67 0.2876940981388749 1103 68-69 0.5020486278861824 1103 70-71 0.6548435406646966 1103 72-73 0.5677101459151892 1103 74-75 0.2573575985319714 1103 76-77 0.03721567127297476 1103 78-79 0.12071996052048917 1103 80-81 -0.06932641041285592 1103 82-83 -0.14328820009665222 1103 84-85 -0.5995908473054925 1103 86-87 -1.1662933562439655 1103 88-89 -1.4062508686979882 1103 90-91 -3.065809801586113 1103 92-93 -9.301855991001666 1103 94-95 -6.048765488837468 1103 96-97 -3.5921923719875366 1103 98-99 -2.54886739810582 1103 100-101 -1.9433798756963068 1104 1 0.9646468272131585 1104 2 0.7851367567468657 1104 3 0.9299240272971794 1104 4 0.9034757149059374 1104 5 1.0519165369976378 1104 6 0.9041179902877872 1104 7 0.9687552532201664 1104 8 0.856945493123014 1104 9 0.9926626643440741 1104 10-11 0.8534115472838053 1104 12-13 0.8932729539011959 1104 14-15 1.1227135895709708 1104 16-17 1.1364077259393284 1104 18-19 1.1777571164908593 1104 20-21 1.2776793634682022 1104 22-23 1.078955522200438 1104 24-25 0.9955486836398677 1104 26-27 0.8757833058954887 1104 28-29 1.0144560517682848 1104 30-31 1.0098797898630139 1104 32-33 0.9638646867004681 1104 34-35 1.1009315268625954 1104 36-37 0.9805095460539732 1104 38-39 0.9230108000486581 1104 40-41 0.7411888098035959 1104 42-43 0.6749482089465175 1104 44-45 0.731321504955325 1104 46-47 0.3251580816954842 1104 48-49 0.27940689372021765 1104 50-51 0.41007302874665896 1104 52-53 0.54736230196756 1104 54-55 0.6608581872848553 1104 56-57 0.5058149484724943 1104 58-59 0.2947719862282163 1104 60-61 0.4375235948333298 1104 62-63 0.4399475949093272 1104 64-65 0.4920207409946862 1104 66-67 0.47248225312082326 1104 68-69 0.40922805052350597 1104 70-71 0.37650239735430446 1104 72-73 0.3919975647787517 1104 74-75 0.3661638964662579 1104 76-77 0.4064843627774195 1104 78-79 0.24519177484499366 1104 80-81 0.1161891797843353 1104 82-83 -0.02118981877659465 1104 84-85 -0.0379881287895536 1104 86-87 0.11954321864449824 1104 88-89 -0.047854875055236334 1104 90-91 -0.7983423317747729 1104 92-93 -4.143340189091173 1104 94-95 -1.9875806358026011 1104 96-97 -1.4445929764924479 1104 98-99 -1.1999907957434104 1104 100-101 -1.2710534391434667 1105 1 1.047996135625631 1105 2 0.9548619840842818 1105 3 0.8731899165143346 1105 4 0.8430278374571145 1105 5 0.8081150954533527 1105 6 0.8094263106165585 1105 7 0.8631252969599998 1105 8 0.868249016633321 1105 9 1.0770229902903452 1105 10-11 0.990441025892359 1105 12-13 0.9186602503360675 1105 14-15 1.161796767090344 1105 16-17 1.032122994978529 1105 18-19 0.9721860565407852 1105 20-21 1.0347624402567277 1105 22-23 0.9811769106384958 1105 24-25 1.061668625184744 1105 26-27 1.0988715769598443 1105 28-29 1.002570202460852 1105 30-31 1.158826644971846 1105 32-33 1.123490085085102 1105 34-35 1.1666559631895694 1105 36-37 1.2598802984235888 1105 38-39 1.262847780059829 1105 40-41 1.2932619716316012 1105 42-43 1.3310373885406293 1105 44-45 1.1295354260362203 1105 46-47 1.1376143291747738 1105 48-49 1.1105618899896648 1105 50-51 0.955806784628976 1105 52-53 0.8293592792613396 1105 54-55 0.955429243721067 1105 56-57 1.0615328685289036 1105 58-59 0.9851968166158258 1105 60-61 0.8655626307688635 1105 62-63 0.7198223919347235 1105 64-65 0.8588779893793834 1105 66-67 0.9932537928308349 1105 68-69 0.8209279967613448 1105 70-71 0.7978392206079796 1105 72-73 0.6550547029842022 1105 74-75 0.4705904509911214 1105 76-77 0.11431386293768853 1105 78-79 0.05434440198742507 1105 80-81 0.033327810805296565 1105 82-83 0.10898488677113605 1105 84-85 0.03779026100836802 1105 86-87 -0.44286100408623597 1105 88-89 -0.5120862499024348 1105 90-91 -1.6859615543434785 1105 92-93 -7.334957098754737 1105 94-95 -3.2772392089931266 1105 96-97 -1.7598516302676472 1105 98-99 -1.0535672873505408 1105 100-101 -0.7052276863268094 1106 1 0.9801289426414783 1106 2 0.9433457873460327 1106 3 0.8692570316578525 1106 4 0.8998967462737539 1106 5 0.7928193230080538 1106 6 0.6591868584178258 1106 7 0.5932357410469677 1106 8 0.581237156918661 1106 9 1.005624415494374 1106 10-11 0.8676252419906021 1106 12-13 0.7549550712982125 1106 14-15 0.8801986092601126 1106 16-17 1.0359155294818763 1106 18-19 0.976428415024138 1106 20-21 0.7487378651075716 1106 22-23 0.8380867604658135 1106 24-25 0.8190175805300512 1106 26-27 0.8801760752875012 1106 28-29 1.0628209178303294 1106 30-31 1.0947757076774849 1106 32-33 1.1517466305950776 1106 34-35 1.0889618294389791 1106 36-37 1.2232919058151097 1106 38-39 1.0656974852581982 1106 40-41 1.0402691486083455 1106 42-43 1.1115953284170885 1106 44-45 0.9423710918064643 1106 46-47 1.3375826130699338 1106 48-49 1.074279456758216 1106 50-51 0.9661819483710516 1106 52-53 1.0211419967077475 1106 54-55 1.1909707413010686 1106 56-57 1.085781412091979 1106 58-59 1.0530038280713967 1106 60-61 1.0028085834512446 1106 62-63 0.9131289759696593 1106 64-65 0.802262326192114 1106 66-67 0.7314676577764558 1106 68-69 0.7513972457573708 1106 70-71 0.8119155483574616 1106 72-73 0.7814809393192625 1106 74-75 0.6863820840893808 1106 76-77 0.6332706051577972 1106 78-79 0.7859552111170949 1106 80-81 0.8423111998248416 1106 82-83 0.8522373074303538 1106 84-85 0.8013664670769458 1106 86-87 0.45202426883479774 1106 88-89 0.05550595303143524 1106 90-91 -0.4878147500705019 1106 92-93 -4.055158895766395 1106 94-95 -1.6345492440214109 1106 96-97 -0.5026458806034775 1106 98-99 -0.4838094279504084 1106 100-101 -0.28572421468724585 1107 1 0.5960233769608401 1107 2 0.7068135603728081 1107 3 0.5864053212975548 1107 4 0.71458989782289 1107 5 0.5440731783283965 1107 6 0.5755640839891143 1107 7 0.5242728222189825 1107 8 0.6981861089725001 1107 9 0.7441035792403383 1107 10-11 0.542920962585022 1107 12-13 0.5690743058418803 1107 14-15 0.7327319490189765 1107 16-17 0.9661896515202884 1107 18-19 0.5603764821067116 1107 20-21 0.541901900226037 1107 22-23 0.7399408190666747 1107 24-25 0.707607803788143 1107 26-27 0.6209452116957479 1107 28-29 0.6995551110169274 1107 30-31 0.775176441940765 1107 32-33 0.6724411577926119 1107 34-35 0.6616337416778677 1107 36-37 0.7661221052403206 1107 38-39 0.7331528862115135 1107 40-41 0.595044488577507 1107 42-43 0.62818875860075 1107 44-45 0.4707837074660759 1107 46-47 0.5177272752036757 1107 48-49 0.5457652008350458 1107 50-51 0.7329390106169456 1107 52-53 0.9338711254053464 1107 54-55 0.6948306617412747 1107 56-57 0.5164128084189166 1107 58-59 0.6030057995420286 1107 60-61 0.5823304799183724 1107 62-63 0.1946179620204127 1107 64-65 0.2374250382347327 1107 66-67 0.17046115630442316 1107 68-69 0.28803856077209034 1107 70-71 0.3319588330903933 1107 72-73 0.44532679660231267 1107 74-75 0.4895848476530986 1107 76-77 0.34829299230205635 1107 78-79 0.3416641519554524 1107 80-81 0.21230410253058096 1107 82-83 0.4009571658349529 1107 84-85 0.3704774652544316 1107 86-87 0.18159085196952063 1107 88-89 -0.02536640081687125 1107 90-91 -0.3704548559165701 1107 92-93 -3.750186627786583 1107 94-95 -1.3083407325255472 1107 96-97 -0.46060580279610264 1107 98-99 -0.36002911111317104 1107 100-101 -0.3751477128647309 1108 1 0.40660988347757865 1108 2 0.550922140745925 1108 3 0.2219678692147511 1108 4 -0.18659365699438268 1108 5 -0.9634194743677398 1108 6 -0.02030705069016392 1108 7 0.16410479257420718 1108 8 0.24750648336554804 1108 9 0.3673122746147328 1108 10-11 0.3424170333747014 1108 12-13 0.207110978471583 1108 14-15 0.2068019560468386 1108 16-17 0.348507805749783 1108 18-19 0.20124785637198528 1108 20-21 0.21536655330551469 1108 22-23 0.15926742269560634 1108 24-25 0.14744736103322253 1108 26-27 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86-87 0.6140594945140698 2311 88-89 0.7157675472919109 2311 90-91 1.4562693156995579 2311 92-93 2.667462930669185 2311 94-95 2.0372364986404428 2311 96-97 1.7272995973289333 2311 98-99 1.4386501636601956 2311 100-101 1.4950885914491163 2312 1 0.08422684016430182 2312 2 0.10501909258569242 2312 3 -0.01614274338286492 2312 4 -0.02825928730570837 2312 5 0.0729294935817748 2312 6 0.08464066916863544 2312 7 0.16390549346930072 2312 8 -0.490441501162465 2312 9 0.03131630523983375 2312 10-11 -0.04628278709666489 2312 12-13 0.10710906520017716 2312 14-15 -0.23675135566653438 2312 16-17 0.1159831484645224 2312 18-19 0.09959734090477212 2312 20-21 0.14066885018783637 2312 22-23 0.05294693060220368 2312 24-25 0.18266072268297506 2312 26-27 0.19620145055493055 2312 28-29 0.26597349655295943 2312 30-31 0.21306278966167014 2312 32-33 0.29004564276841904 2312 34-35 0.25893925479220314 2312 36-37 0.38627783798796855 2312 38-39 0.5533137914827151 2312 40-41 0.6287438774239504 2312 42-43 0.6438857760440797 2312 44-45 0.48923250744061164 2312 46-47 0.8497006327285064 2312 48-49 0.5652970476646821 2312 50-51 0.618593613367139 2312 52-53 0.4763486846108478 2312 54-55 0.6815575684130373 2312 56-57 0.6514252224754422 2312 58-59 0.7796057008661919 2312 60-61 0.8472619638755923 2312 62-63 0.6693485929091274 2312 64-65 0.6541434301369513 2312 66-67 0.8558833933810739 2312 68-69 0.705306831716829 2312 70-71 0.6407744047736124 2312 72-73 0.6126523631925842 2312 74-75 0.7122329409813375 2312 76-77 0.9326540662771023 2312 78-79 0.9661063151843798 2312 80-81 0.8910275237172343 2312 82-83 1.1286803005433832 2312 84-85 1.0462716465633193 2312 86-87 0.9507606221503231 2312 88-89 1.2737518828602319 2312 90-91 1.7798992630037276 2312 92-93 2.7817139165220475 2312 94-95 2.2238248041436677 2312 96-97 1.8282430321924394 2312 98-99 1.7378955132820444 2312 100-101 1.673693243972549 2313 1 -0.3310938456481871 2313 2 -0.9620228770599404 2313 3 -0.3676269750503174 2313 4 -0.14730761151314198 2313 5 -0.9190625422868308 2313 6 -0.5356173990161395 2313 7 -1.090181415390994 2313 8 -1.1686466415180377 2313 9 -1.3026851811242395 2313 10-11 -2.2583577971466013 2313 12-13 -0.5415869701882414 2313 14-15 -0.004568572374779478 2313 16-17 -1.3352810705190805 2313 18-19 -0.8789016169818424 2313 20-21 -1.2547572039894206 2313 22-23 -1.0755712386584975 2313 24-25 -0.23099224605677904 2313 26-27 -0.9138493164389487 2313 28-29 -2.665893651562982 2313 30-31 -0.7349599788770078 2313 32-33 -0.3319478660380071 2313 34-35 0.026333643570396248 2313 36-37 -0.1581467369332401 2313 38-39 -0.44324296953884357 2313 40-41 -0.3263041727301683 2313 42-43 -0.03676363158217555 2313 44-45 -0.4894195688488807 2313 46-47 -0.9878531808378455 2313 48-49 -0.5396245983611117 2313 50-51 -0.4081828562733598 2313 52-53 -0.1931299901715846 2313 54-55 -0.0549856834388045 2313 56-57 -0.2899724849540881 2313 58-59 -0.29880711187641396 2313 60-61 -0.26419075955881155 2313 62-63 -0.3869588980656715 2313 64-65 -0.12245830090065724 2313 66-67 -0.3141436841918086 2313 68-69 -0.24663689337057804 2313 70-71 -0.199587862903023 2313 72-73 0.044311700710164104 2313 74-75 0.049352178655059475 2313 76-77 -0.14033059542501292 2313 78-79 0.10619889307830732 2313 80-81 0.07854702138619984 2313 82-83 -0.05166997828809272 2313 84-85 0.21498305105100002 2313 86-87 0.13643006252082657 2313 88-89 0.16711967425116825 2313 90-91 0.9752705460076001 2313 92-93 2.1637413700297365 2313 94-95 1.3409227691201266 2313 96-97 0.923588410445241 2313 98-99 0.6859532295413011 2313 100-101 0.6227681955788409 2314 1 -0.6806415950777982 2314 2 -0.8589576012947049 2314 3 -2.060941526694311 2314 4 -0.6879488667932776 2314 5 -0.4076585196888871 2314 6 -3.008628213926496 2314 7 -3.9594142647154342 2314 8 -1.5978818244286188 2314 9 -1.8590945015934182 2314 10-11 -1.3593683739681026 2314 12-13 -2.449075160350283 2314 14-15 -3.3607026912962183 2314 16-17 -2.3377368545803705 2314 18-19 -2.795707497303553 2314 20-21 -2.365675470134917 2314 22-23 -3.4721270507858897 2314 24-25 -1.986325692653132 2314 26-27 -2.190776318553368 2314 28-29 -1.4823462616317755 2314 30-31 -1.4562586432142908 2314 32-33 -2.3863626996044403 2314 34-35 -0.9018862125671774 2314 36-37 -0.5820723215517489 2314 38-39 -0.7802447370726782 2314 40-41 -0.9139666959674813 2314 42-43 -0.8571545624465315 2314 44-45 -1.4877135281719518 2314 46-47 -1.6800501142218423 2314 48-49 -2.148336699266636 2314 50-51 -1.318298597415584 2314 52-53 -0.7964939610206798 2314 54-55 -0.46600992671831065 2314 56-57 -0.4453595606710721 2314 58-59 -0.5857929739709355 2314 60-61 -0.368953995170596 2314 62-63 -0.541106650333429 2314 64-65 -0.9010393110281036 2314 66-67 -0.6848880429838324 2314 68-69 -0.5810895531069171 2314 70-71 -0.5730837444388364 2314 72-73 -0.5409022323758279 2314 74-75 -0.39464796246383216 2314 76-77 -0.42100749702178675 2314 78-79 -0.4001078993917808 2314 80-81 -0.37861639823200477 2314 82-83 0.034196235484163395 2314 84-85 0.010808553205627902 2314 86-87 0.1606984714407993 2314 88-89 0.615177574780482 2314 90-91 1.1608126199349798 2314 92-93 1.8920151706374142 2314 94-95 1.154452385166369 2314 96-97 1.0564553944585988 2314 98-99 0.9497954948858123 2314 100-101 0.9888194643086319 2315 1 -7.136119480182018 2315 2 -3.2088397842983944 2315 3 -1.5962335675913337 2315 4 -1.1348006917462712 2315 5 -1.6836337377713733 2315 6 -1.8501587051265354 2315 7 -3.3642475859442804 2315 8 -3.1298396846782985 2315 9 -3.7698065681519495 2315 10-11 -2.4728315884353833 2315 12-13 -3.071883744044733 2315 14-15 -4.847458668894852 2315 16-17 -4.619159696552273 2315 18-19 -3.4603044986189104 2315 20-21 -4.534646427438361 2315 22-23 -5.88596621041178 2315 24-25 -3.9033509547152576 2315 26-27 -4.737062157918764 2315 28-29 -3.200161442271053 2315 30-31 -2.9782922371975573 2315 32-33 -1.9831888025248503 2315 34-35 -2.7707783898414107 2315 36-37 -3.253495950315237 2315 38-39 -2.3361710933803224 2315 40-41 -3.6653013164111563 2315 42-43 -8.053993072465016 2315 44-45 -4.577455379835513 2315 46-47 -3.28427811028385 2315 48-49 -6.053558070140017 2315 50-51 -7.22548644289779 2315 52-53 -7.508667139900773 2315 54-55 -5.263279457306343 2315 56-57 -4.692019731263628 2315 58-59 -4.734483571206269 2315 60-61 -3.6621954554557767 2315 62-63 -3.7362033248276596 2315 64-65 -2.5026047236700286 2315 66-67 -3.0741604423350317 2315 68-69 -2.5087018020397025 2315 70-71 -2.165305906138631 2315 72-73 -2.395858010653921 2315 74-75 -3.0671930718480347 2315 76-77 -2.934384870509735 2315 78-79 -2.1897063128971546 2315 80-81 -2.0120085391927773 2315 82-83 -2.376693060922417 2315 84-85 -2.353884779643529 2315 86-87 -2.692626126488527 2315 88-89 -2.169328985850889 2315 90-91 -1.2638009443519422 2315 92-93 -0.31747333413578716 2315 94-95 -0.5318986973781534 2315 96-97 -0.7924297823879378 2315 98-99 -0.7168885215440106 2315 100-101 -0.4504276164928491 2316 1 -4.615403823477877 2316 2 -3.068788818294589 2316 3 -4.9752086765088634 2316 4 -4.766605262582921 2316 5 -4.141814194855041 2316 6 -4.5997461024655095 2316 7 -3.1322016773697214 2316 8 -1.7749741940162593 2316 9 -1.9966396686346286 2316 10-11 -3.2520620714155193 2316 12-13 -4.431477517574621 2316 14-15 -3.3107896954833507 2316 16-17 -3.1594474303278055 2316 18-19 -3.1125854557874497 2316 20-21 -6.007612683678545 2316 22-23 -6.745684352307052 2316 24-25 -4.655986845923948 2316 26-27 -4.236840296941487 2316 28-29 -4.788291063921378 2316 30-31 -6.2474059031373805 2316 32-33 -4.682776743907418 2316 34-35 -6.413840963559313 2316 36-37 -9.807557888590225 2316 38-39 -6.5702068958011 2316 40-41 -5.995279750523125 2316 42-43 -4.972694244552532 2316 44-45 -3.61010169491945 2316 46-47 -6.09814621670748 2316 48-49 -3.7087209350300547 2316 50-51 -4.625961284145866 2316 52-53 -5.6498457994644085 2316 54-55 -7.358620376783541 2316 56-57 -5.329674729283308 2316 58-59 -5.9883171199808665 2316 60-61 -6.54614540779756 2316 62-63 -6.987736113246932 2316 64-65 -5.693250002894963 2316 66-67 -6.947779006843366 2316 68-69 -5.703636103335395 2316 70-71 -6.748081268362856 2316 72-73 -8.67183456890358 2316 74-75 -8.334201397891185 2316 76-77 -7.895681935720976 2316 78-79 -6.906503005982579 2316 80-81 -6.744106131908687 2316 82-83 -5.566853706114596 2316 84-85 -6.869111580536629 2316 86-87 -5.620070187926284 2316 88-89 -5.5235121370384235 2316 90-91 -5.7768325293467875 2316 92-93 -4.486658813829905 2316 94-95 -4.39543243460945 2316 96-97 -5.0807567830538485 2316 98-99 -6.117453021176235 2316 100-101 -5.048067664194592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13422.0 3 5520.0 4 5622.0 5 6141.0 6 6947.0 7 8099.0 8 9903.0 9 11997.0 10 14139.0 11 16626.0 12 18965.0 13 20862.0 14 22998.0 15 25161.0 16 27711.0 17 30144.0 18 29223.0 19 28621.0 20 30367.0 21 33178.0 22 36737.0 23 40695.0 24 44986.0 25 50574.0 26 57143.0 27 64761.0 28 71877.0 29 81364.0 30 93395.0 31 105220.0 32 121437.0 33 140673.0 34 164331.0 35 195756.0 36 223443.0 37 193303.0 38 60869.0 39 1168.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11353113307132 18.947554860136666 13.28979914254472 23.6491148642473 2 15.327565187655765 20.303301275098683 40.62114289990461 23.747990637340944 3 19.427389303664018 24.855597001494704 29.562215953760727 26.154797741080543 4 12.43382309061441 16.299909159196847 37.91310541985217 33.353162330336566 5 13.317814992327657 37.80831454714229 34.885862049368214 13.98800841116184 6 31.8099618004865 36.446086219053164 17.91086969595233 13.833082284508006 7 28.08008429926238 32.65331928345627 21.504789192645127 17.76180722463622 8 25.892553126499152 35.41040466260121 20.183499707095674 18.51354250380397 9 26.59562226438879 16.318224243523442 20.15360857988068 36.93254491220709 10-11 24.630348941506526 26.591102611970342 28.236122042862572 20.542426403660567 12-13 25.684476825451096 23.96598873505306 28.272994649304117 22.076539790191724 14-15 22.700880372324228 25.398355571152738 26.283274441355005 25.617489615168036 16-17 22.211834797320897 28.531975074916144 26.94921093852673 22.306979189236227 18-19 22.018821788794543 27.385413800403413 28.968940875372784 21.626823535429264 20-21 22.996951861387018 26.665825158428135 28.834382416235393 21.502840563949455 22-23 22.366900567315852 26.730848619534587 28.6601441014949 22.242106711654667 24-25 22.173242209662934 26.817441296041455 29.040659543454588 21.968656950841023 26-27 22.18718823894256 27.004267491372264 28.75696431706279 22.051579952622387 28-29 22.060033513313996 27.171228490628664 28.88522625751331 21.88351173854403 30-31 22.266717303084786 27.034327179650226 28.79362682397124 21.905328693293747 32-33 22.031479464717854 26.975023814876735 28.765694068917092 22.22780265148832 34-35 22.27160665794424 27.098339067344845 28.744418488793627 21.885635785917287 36-37 22.44062562261039 26.79724595660081 28.760412770107784 22.001715650681014 38-39 22.182075054016273 27.13060768988962 28.762667855526097 21.924649400568008 40-41 22.37854470386333 27.041262644746144 28.75811745031226 21.822075201078263 42-43 22.168309167209113 27.082782595842538 28.58263620626434 22.166272030684013 44-45 22.251984244471235 27.248230108408077 28.486668672030618 22.013116975090064 46-47 22.431107202792763 27.13880142026099 28.112364363085725 22.317727013860527 48-49 22.222369650638882 27.2224122995737 28.247668998773605 22.30754905101381 50-51 22.262463167647482 27.482935972427423 28.085073107850818 22.16952775207428 52-53 22.37004874238578 27.478567379570666 27.923618918339915 22.22776495970364 54-55 22.35231864286447 27.395889095289583 27.993361054060635 22.25843120778531 56-57 22.27970313976581 27.69863194784501 27.828905707525813 22.192759204863364 58-59 22.20536639202771 27.48321003756792 28.016845512447112 22.294578057957263 60-61 22.24583879333297 27.52491228469125 28.046537673286885 22.18271124868889 62-63 22.223224965950138 27.397903712915262 28.13589151418251 22.242979806952093 64-65 22.13010293197812 27.40845458035579 28.11288057725843 22.34856191040766 66-67 22.10738051231325 27.653796894323285 27.814062073266722 22.424760520096747 68-69 22.23080712857779 27.44113996608146 27.94056202450093 22.387490880839817 70-71 22.12414997449953 27.266219447110036 27.93858852239647 22.671042055993958 72-73 22.113943348202458 27.284665022896053 27.888686747964282 22.712704880937203 74-75 22.02008702703753 27.612410713397058 27.61570467871042 22.751797580854998 76-77 22.095566444792603 27.412439056123226 27.74229967762813 22.74969482145604 78-79 22.13717302797776 27.401120437558358 27.76353269601733 22.698173838446554 80-81 22.04901387128248 27.460190952608805 27.617623645570493 22.873171530538222 82-83 22.065947556205437 27.472357932630498 27.703282210370027 22.75841230079404 84-85 22.182371162038432 27.33697955922574 27.567737653707425 22.912911625028404 86-87 21.950247473669272 27.221155853322713 27.812301487353597 23.016295185654418 88-89 22.11064660770317 27.17506090682441 27.69838561366588 23.01590687180654 90-91 21.76430234935738 27.25348968832267 27.95621895834292 23.02598900397703 92-93 21.493523252895834 27.173911750678243 28.331793091390463 23.00077190503546 94-95 21.903157156331613 27.191903536337396 27.650286044714928 23.254653262616067 96-97 21.92920791093608 26.973444078601204 27.692969141812657 23.40437886865006 98-99 21.862045668703367 26.947545588697203 27.56189810177969 23.628510640819737 100-101 21.92362237541411 26.966776937640557 27.654504617488374 23.455096069456964 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1805.0 1 1079.0 2 366.0 3 371.5 4 342.5 5 328.5 6 474.0 7 818.5 8 1251.5 9 1457.5 10 1376.5 11 1305.5 12 1319.5 13 1421.5 14 1596.5 15 1735.5 16 1908.0 17 2155.5 18 2362.5 19 2641.5 20 3132.5 21 3775.5 22 4652.0 23 5808.0 24 7202.5 25 9176.5 26 12139.0 27 15800.0 28 19209.0 29 22580.5 30 25797.0 31 29507.0 32 33999.0 33 38381.5 34 42974.5 35 47723.5 36 53060.0 37 57983.5 38 61161.0 39 63266.0 40 65751.0 41 68353.5 42 69987.5 43 71606.5 44 74262.5 45 76515.0 46 77825.0 47 79338.5 48 81682.5 49 82011.0 50 79432.5 51 77115.5 52 75303.0 53 73033.5 54 69870.0 55 64852.0 56 58777.5 57 53145.0 58 47798.5 59 41966.5 60 35234.5 61 28258.0 62 21834.0 63 16330.0 64 11708.5 65 8353.0 66 6025.0 67 4375.0 68 3172.0 69 2239.5 70 1565.5 71 1062.5 72 681.0 73 441.5 74 295.0 75 201.0 76 134.0 77 83.0 78 51.0 79 37.0 80 28.0 81 18.5 82 13.5 83 9.5 84 5.0 85 6.5 86 7.5 87 4.5 88 5.0 89 4.5 90 4.5 91 4.5 92 4.5 93 3.5 94 3.0 95 2.0 96 1.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08427266679221607 2 0.09520303514089766 3 0.09113372051757897 4 0.09425668290291657 5 0.08980882738440545 6 0.07433596829341461 7 0.08767953484894798 8 0.0836102202256293 9 0.07793210679774276 10-11 0.08479316052310566 12-13 0.08439096082196369 14-15 0.08919369842971774 16-17 0.08756124081920036 18-19 0.08938296887731395 20-21 0.09887015006307437 22-23 0.10866489572617866 24-25 0.13090417331873427 26-27 0.13662960435851987 28-29 0.1359671577919331 30-31 0.13757595659650096 32-33 0.1337905476445766 34-35 0.1290114688427721 36-37 0.13289151301849456 38-39 0.1264090001883241 40-41 0.12162992138651958 42-43 0.12170089780436817 44-45 0.11228469303645632 46-47 0.1315429610793715 48-49 0.14791485479644437 50-51 0.15664495419181992 52-53 0.1660848177656813 54-55 0.16109280971033105 56-57 0.13355395958508132 58-59 0.12621972974072787 60-61 0.12170089780436817 62-63 0.1184359825833334 64-65 0.10535266289324484 66-67 0.11836500616548484 68-69 0.11536033780989487 70-71 0.12456361332426097 72-73 0.1508958643460848 74-75 0.16367161955882953 76-77 0.16785922821189583 78-79 0.1650438303039021 80-81 0.16975193268785801 82-83 0.17013047358305045 84-85 0.1494999947950627 86-87 0.14921608912366835 88-89 0.17024876761279809 90-91 0.2614298057422761 92-93 0.5411005508716377 94-95 0.16882923925582646 96-97 0.17942838432121466 98-99 0.19710151236551152 100-101 0.20493257713480503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2113378.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.47476508362497 #Duplication Level Percentage of deduplicated Percentage of total 1 82.39187830670491 56.41764511850236 2 10.75668723731716 14.731232633066387 3 3.2296853646922106 6.634558399239624 4 1.287127244302927 3.5254294274550597 5 0.6898527550446765 2.3618752671987853 6 0.41481672671389924 1.704268674869551 7 0.25984532301614627 1.2454993216126486 8 0.1751853072711057 0.9596618209193297 9 0.13258997807861425 0.8171160841238485 >10 0.6137622627208016 7.396792841962882 >50 0.03264186088316964 1.5062829429874813 >100 0.015299485322601796 1.9056307411335547 >500 4.187652878442562E-4 0.21257625625244622 >1k 1.3958842928141776E-4 0.3147065304456724 >5k 6.979421464070888E-5 0.2667239402304173 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5581 0.2640795920086232 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3932 0.1860528499870823 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2653 0.1255336243681916 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.73176118990545E-5 0.0 2 0.0 0.0 0.0 4.73176118990545E-5 4.73176118990545E-5 3 0.0 0.0 0.0 4.73176118990545E-5 9.4635223798109E-5 4 0.0 0.0 0.0 9.4635223798109E-5 9.4635223798109E-5 5 0.0 4.73176118990545E-5 0.0 9.4635223798109E-5 9.4635223798109E-5 6 0.0 4.73176118990545E-5 0.0 9.4635223798109E-5 9.4635223798109E-5 7 0.0 4.73176118990545E-5 0.0 9.4635223798109E-5 9.4635223798109E-5 8 0.0 4.73176118990545E-5 0.0 9.4635223798109E-5 9.4635223798109E-5 9 0.0 4.73176118990545E-5 0.0 2.365880594952725E-4 9.4635223798109E-5 10-11 4.73176118990545E-5 4.73176118990545E-5 0.0 2.6024686544479976E-4 9.4635223798109E-5 12-13 4.73176118990545E-5 9.4635223798109E-5 0.0 3.312232832933815E-4 1.89270447596218E-4 14-15 4.73176118990545E-5 9.4635223798109E-5 0.0 3.5488208924290874E-4 2.1292925354574527E-4 16-17 4.73176118990545E-5 9.4635223798109E-5 0.0 4.73176118990545E-4 2.365880594952725E-4 18-19 4.73176118990545E-5 9.4635223798109E-5 0.0 5.914701487381812E-4 2.365880594952725E-4 20-21 4.73176118990545E-5 9.4635223798109E-5 0.0 7.57081790384872E-4 3.312232832933815E-4 22-23 4.73176118990545E-5 9.4635223798109E-5 0.0 0.001230257909375417 3.312232832933815E-4 24-25 4.73176118990545E-5 9.4635223798109E-5 0.0 0.0016797752224164348 3.78540895192436E-4 26-27 4.73176118990545E-5 1.419528356971635E-4 0.0 0.0020346573116593433 4.258585070914905E-4 28-29 4.73176118990545E-5 1.419528356971635E-4 0.0 0.00352516208647956 4.258585070914905E-4 30-31 4.73176118990545E-5 1.419528356971635E-4 0.0 0.008990346260820355 4.495173130410178E-4 32-33 4.73176118990545E-5 1.419528356971635E-4 0.0 0.021979030727110813 4.73176118990545E-4 34-35 9.4635223798109E-5 1.419528356971635E-4 0.0 0.03875312414532564 5.67811342788654E-4 36-37 9.4635223798109E-5 1.419528356971635E-4 0.0 0.06508537516714946 5.67811342788654E-4 38-39 9.4635223798109E-5 1.419528356971635E-4 0.0 0.10558925095274012 6.151289546877085E-4 40-41 9.4635223798109E-5 1.419528356971635E-4 0.0 0.1491451127058198 6.62446566586763E-4 42-43 9.4635223798109E-5 1.419528356971635E-4 0.0 0.19348171505523384 6.62446566586763E-4 44-45 9.4635223798109E-5 1.419528356971635E-4 0.0 0.24576767620368906 6.62446566586763E-4 46-47 9.4635223798109E-5 1.419528356971635E-4 0.0 0.312982343906296 7.57081790384872E-4 48-49 1.419528356971635E-4 1.419528356971635E-4 0.0 0.38715270055806394 8.51717014182981E-4 50-51 1.419528356971635E-4 1.419528356971635E-4 0.0 0.4647772428784628 8.51717014182981E-4 52-53 1.419528356971635E-4 1.419528356971635E-4 0.0 0.5420942207215178 8.990346260820354E-4 54-55 1.419528356971635E-4 1.419528356971635E-4 0.0 0.6289930149741314 8.990346260820354E-4 56-57 1.89270447596218E-4 1.419528356971635E-4 0.0 0.7317668680188778 9.226934320315627E-4 58-59 1.89270447596218E-4 1.419528356971635E-4 0.0 0.8475057467239652 9.4635223798109E-4 60-61 1.89270447596218E-4 1.419528356971635E-4 0.0 0.9756181809406552 9.4635223798109E-4 62-63 1.89270447596218E-4 1.419528356971635E-4 0.0 1.1027606041134148 9.4635223798109E-4 64-65 1.89270447596218E-4 1.419528356971635E-4 0.0 1.2343982004165843 9.4635223798109E-4 66-67 1.89270447596218E-4 1.419528356971635E-4 0.0 1.3829281841677163 9.700110439306173E-4 68-69 1.89270447596218E-4 1.419528356971635E-4 0.0 1.5571279723740856 9.936698498801445E-4 70-71 1.89270447596218E-4 1.419528356971635E-4 0.0 1.7547972960823857 9.936698498801445E-4 72-73 1.89270447596218E-4 1.419528356971635E-4 0.0 1.9582157096364208 0.001040987461779199 74-75 1.89270447596218E-4 1.419528356971635E-4 0.0 2.1679746831849296 0.0010646462677287262 76-77 1.89270447596218E-4 1.419528356971635E-4 0.0 2.392851633735186 0.0011119638796277809 78-79 1.89270447596218E-4 1.6561164164669075E-4 0.0 2.65139506515162 0.001135622685577308 80-81 1.89270447596218E-4 1.89270447596218E-4 2.365880594952725E-5 2.9358212302768365 0.0011829402974763625 82-83 1.89270447596218E-4 1.89270447596218E-4 4.73176118990545E-5 3.2303260467365518 0.0012539167153249441 84-85 1.89270447596218E-4 2.365880594952725E-4 4.73176118990545E-5 3.515769540517598 0.0012775755212744715 86-87 1.89270447596218E-4 2.365880594952725E-4 4.73176118990545E-5 3.8230027945781586 0.001324893133173526 88-89 1.89270447596218E-4 2.365880594952725E-4 4.73176118990545E-5 4.150298716083919 0.0013722107450725805 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4925 0.0 36.22878 1 GTATCAA 7350 0.0 32.087887 1 ATCAACG 8775 0.0 26.768244 3 TATCAAC 8895 0.0 26.354395 2 TCAACGC 8965 0.0 26.25324 4 CAACGCA 9120 0.0 25.85908 5 AACGCAG 9335 0.0 25.209686 6 GTACATG 7635 0.0 24.17757 1 TACATGG 7375 0.0 24.000422 2 ACATGGG 7560 0.0 22.722105 3 ACGCAGA 10575 0.0 21.940113 7 CGCAGAG 10570 0.0 21.771967 8 CATGGGG 3165 0.0 20.090122 4 GTGTAGC 1625 0.0 19.85764 1 GAGTACT 5550 0.0 19.152573 12-13 GCAGAGT 11040 0.0 19.127256 9 CATGGGA 5060 0.0 17.9116 4 GTACTTT 6105 0.0 17.68474 14-15 AGAGTAC 9515 0.0 17.33078 10-11 AGTACAT 5425 0.0 17.319588 2 >>END_MODULE