FastQCFastQC Report
Fri 27 May 2016
SRR939619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113378
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58970.2790319573687244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47130.22300790488024386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33120.15671593060966849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45200.035.7199941
GTATCAA70450.034.0757061
ATCAACG83100.028.5398333
TCAACGC84600.028.0338064
CAACGCA85800.027.6417265
TATCAAC86750.027.5050132
AACGCAG89350.026.5571356
TACATGG72350.026.0192532
GTACATG76800.025.129181
ACATGGG73350.024.8274863
ACGCAGA104800.022.4178077
CGCAGAG104750.022.3403788
CATGGGG28600.021.7184984
CATGGGA48100.021.2293424
GCAGAGT113350.019.6918859
GAGTACT57600.019.09011712-13
AGAGTAC97150.018.11441210-11
AGTACTT62200.017.86717812-13
GTACTTT62550.017.24071114-15
GGACCGA3051.0507938E-716.946396
Produced by FastQC (version 0.11.5)