##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939619_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2113378 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.530270022684064 31.0 31.0 34.0 30.0 34.0 2 31.741007997622763 31.0 31.0 34.0 30.0 34.0 3 31.79026941701863 31.0 31.0 34.0 30.0 34.0 4 35.579810615990134 37.0 35.0 37.0 33.0 37.0 5 35.31147906337626 37.0 35.0 37.0 33.0 37.0 6 35.32958751345003 37.0 35.0 37.0 32.0 37.0 7 35.25498041524043 37.0 35.0 37.0 32.0 37.0 8 35.215250655585514 37.0 35.0 37.0 32.0 37.0 9 36.83518849917052 39.0 37.0 39.0 32.0 39.0 10-11 36.70984745748276 39.0 35.0 39.0 32.0 39.0 12-13 36.65306821590838 39.0 35.0 39.0 32.0 39.0 14-15 37.730568549497534 39.5 37.0 41.0 32.0 41.0 16-17 37.69031734975948 39.0 37.0 41.0 32.0 41.0 18-19 37.65891643615103 39.0 36.5 41.0 32.0 41.0 20-21 37.588872411844925 39.0 36.0 41.0 32.0 41.0 22-23 37.456037916548766 39.0 36.0 41.0 32.0 41.0 24-25 37.337557218822184 39.0 36.0 41.0 31.5 41.0 26-27 37.08420145378631 39.0 36.0 40.0 31.0 41.0 28-29 36.88204097894461 39.0 36.0 40.0 30.5 41.0 30-31 36.653217029797794 38.5 35.5 40.0 30.0 41.0 32-33 36.49467132713599 38.0 35.0 40.0 30.0 41.0 34-35 36.49388514501428 38.0 35.0 40.0 30.0 41.0 36-37 36.41181463987985 38.0 35.0 40.0 30.0 41.0 38-39 36.45676826388843 38.5 35.0 40.0 30.0 41.0 40-41 36.36655581727453 39.0 35.0 40.0 30.0 41.0 42-43 36.18721946570845 38.0 35.0 40.0 30.0 41.0 44-45 35.98655541034306 38.0 35.0 40.0 29.0 41.0 46-47 35.722825495486376 38.0 35.0 40.0 28.0 41.0 48-49 35.44615161130663 38.0 34.0 40.0 27.0 41.0 50-51 35.21794799605182 38.0 34.0 40.0 27.0 41.0 52-53 34.97947385654625 38.0 34.0 40.0 26.0 41.0 54-55 34.69147970689579 38.0 33.5 40.0 26.0 41.0 56-57 32.60194721436487 37.0 30.5 39.5 16.5 41.0 58-59 27.49815697901653 32.5 17.5 38.5 7.0 40.0 60-61 28.285988119494004 33.0 20.0 38.0 9.0 40.0 62-63 31.42268609780172 34.5 29.5 38.5 13.5 40.0 64-65 31.659867993326323 35.5 31.0 39.0 10.0 40.0 66-67 24.875429525622014 27.0 18.0 35.5 2.0 39.0 68-69 17.306336585315073 13.0 7.5 28.0 2.0 35.0 70-71 16.1693568306285 12.5 7.5 26.5 2.0 33.5 72-73 17.262605648397965 13.0 7.0 28.0 2.0 34.0 74-75 18.29301549462519 17.0 8.5 29.0 2.0 34.5 76-77 19.536241505305725 21.0 8.0 30.0 2.0 34.5 78-79 22.64987640639772 27.5 10.5 33.5 2.0 35.5 80-81 26.3403842095451 31.5 22.5 34.5 2.0 36.0 82-83 27.680679698567886 33.5 26.5 35.0 2.0 36.0 84-85 27.73991212173118 34.0 27.0 35.0 2.0 36.0 86-87 27.494756498837404 34.0 27.0 35.0 2.0 36.0 88-89 27.065741197268068 33.0 26.0 35.0 2.0 35.5 90-91 25.69033816950872 32.5 20.0 35.0 2.0 35.0 92-93 23.132778660514116 31.5 2.0 34.5 2.0 35.0 94-95 15.684506273842162 4.5 2.0 32.0 2.0 34.5 96-97 13.093380124142486 2.0 2.0 30.0 2.0 34.0 98-99 12.722013998442304 2.0 2.0 29.5 2.0 34.0 100-101 12.446302317900535 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3803562799498472 1101 2 0.4215710047040453 1101 3 0.4503105476751017 1101 4 0.3223707741854227 1101 5 0.32888168036275545 1101 6 0.4016342497010612 1101 7 0.5440780205001516 1101 8 0.4291049249849763 1101 9 0.5504411420602722 1101 10-11 0.5572603207759883 1101 12-13 0.5995091165345414 1101 14-15 0.7052372509615736 1101 16-17 0.6771637377993329 1101 18-19 0.5942473038202181 1101 20-21 0.6748192601412484 1101 22-23 0.744226760210708 1101 24-25 0.6553786455451771 1101 26-27 0.6304470285425694 1101 28-29 0.6934959653633115 1101 30-31 0.5199656872567644 1101 32-33 0.5383840903628752 1101 34-35 0.5969622909524404 1101 36-37 0.4001765343783106 1101 38-39 0.37694477789894876 1101 40-41 0.5560350961498557 1101 42-43 0.6226889108385478 1101 44-45 0.5625120947769418 1101 46-47 0.6805378870793959 1101 48-49 0.7282064325605972 1101 50-51 0.6040527162449436 1101 52-53 0.7420686288198795 1101 54-55 0.6670419638523626 1101 56-57 -1.3739909163147033 1101 58-59 -9.672538294752034 1101 60-61 -9.8638938478088 1101 62-63 -3.4384975622153995 1101 64-65 -4.66318580443011 1101 66-67 -9.539852906808576 1101 68-69 -4.736564304139373 1101 70-71 -4.083307254151748 1101 72-73 -4.440653924440554 1101 74-75 -6.418240103460498 1101 76-77 -7.854175460466607 1101 78-79 -10.958364334056148 1101 80-81 -7.895517655790087 1101 82-83 -3.9971989039919436 1101 84-85 -3.179852255516323 1101 86-87 -2.8747577738324175 1101 88-89 -2.9411830949111035 1101 90-91 -9.496120644667853 1101 92-93 -16.943435592804448 1101 94-95 -10.999923742237936 1101 96-97 -9.341824262872073 1101 98-99 -9.297919547826774 1101 100-101 -9.099791283765738 1102 1 0.3728735201905984 1102 2 0.28026146573308 1102 3 0.40836352073221605 1102 4 0.2622746203392694 1102 5 0.21115837089689649 1102 6 0.34016400621509035 1102 7 0.3893100090475343 1102 8 0.4999623734293408 1102 9 0.5360077469356526 1102 10-11 0.48105568548608346 1102 12-13 0.40345724369252167 1102 14-15 0.5140032412259075 1102 16-17 0.5423614463331319 1102 18-19 0.48962486745109146 1102 20-21 0.616252853375336 1102 22-23 0.6360340787046965 1102 24-25 0.5224433589468163 1102 26-27 0.5131112412461007 1102 28-29 0.400371484221445 1102 30-31 0.36326551886371305 1102 32-33 0.4617017223415161 1102 34-35 0.335628041362817 1102 36-37 0.2700444083335185 1102 38-39 0.2513262754178669 1102 40-41 0.004267204977992378 1102 42-43 0.1440453769901353 1102 44-45 0.2986855573059515 1102 46-47 0.23006997137969876 1102 48-49 0.0376397156590329 1102 50-51 0.08947648773038708 1102 52-53 -0.04900120358893645 1102 54-55 -0.16796822463464167 1102 56-57 -2.8616336907434885 1102 58-59 -10.538612755611265 1102 60-61 -10.788042389817441 1102 62-63 -4.502938268522829 1102 64-65 -5.71774137885323 1102 66-67 -7.149883702218098 1102 68-69 -4.55993276063076 1102 70-71 -4.671066948544308 1102 72-73 -4.9165451517287195 1102 74-75 -5.594149815069713 1102 76-77 -8.011972895892356 1102 78-79 -10.515575371347897 1102 80-81 -6.234027188629089 1102 82-83 -3.677062108223943 1102 84-85 -3.3204066495371904 1102 86-87 -3.0603919481876147 1102 88-89 -3.0767958074070947 1102 90-91 -9.142089293844286 1102 92-93 -13.663605524739694 1102 94-95 -10.410972885178635 1102 96-97 -9.125643200250613 1102 98-99 -9.312989870792379 1102 100-101 -9.187308205616269 1103 1 0.242845404980919 1103 2 0.19510987495013055 1103 3 0.2118900592336459 1103 4 0.12305589722792831 1103 5 0.3194989125278056 1103 6 0.2183343180580124 1103 7 0.18515666206117487 1103 8 0.18627230629245872 1103 9 0.4275438949809782 1103 10-11 0.4105841773506853 1103 12-13 0.29402696701925635 1103 14-15 0.5123448199399405 1103 16-17 0.5165016075482782 1103 18-19 0.4153991183864676 1103 20-21 0.3113537182570809 1103 22-23 0.4183058833774993 1103 24-25 0.48130534825460103 1103 26-27 0.37263297497557346 1103 28-29 0.3826310946200877 1103 30-31 0.26718557912850116 1103 32-33 0.2820435950857174 1103 34-35 0.45512201317466605 1103 36-37 0.3386137236649134 1103 38-39 0.29148771849054356 1103 40-41 0.28331573249089814 1103 42-43 0.3039680402196083 1103 44-45 0.3660344195345857 1103 46-47 0.2831603081581946 1103 48-49 0.18468830243382683 1103 50-51 0.13628107505677178 1103 52-53 0.2196456080642264 1103 54-55 0.1461806663132137 1103 56-57 -2.7481259678931274 1103 58-59 -10.029333596765458 1103 60-61 -10.133071855514494 1103 62-63 -4.286093417298673 1103 64-65 -5.336986450713976 1103 66-67 -6.714413635742208 1103 68-69 -4.294170179729729 1103 70-71 -4.314738633097809 1103 72-73 -4.749550037415947 1103 74-75 -5.2022841936916695 1103 76-77 -6.967741984205112 1103 78-79 -9.407695135075286 1103 80-81 -5.207040462153497 1103 82-83 -2.9780018650906257 1103 84-85 -2.4785290048327546 1103 86-87 -2.3800531069171775 1103 88-89 -2.4145836976950967 1103 90-91 -5.412207054063824 1103 92-93 -10.688892574534501 1103 94-95 -8.998577576441267 1103 96-97 -8.226440842538988 1103 98-99 -8.435542668010717 1103 100-101 -8.493034044143565 1104 1 0.2811104876027706 1104 2 0.3024653025671036 1104 3 0.26004927544561696 1104 4 0.18890751644447334 1104 5 0.3169928904062047 1104 6 0.2068947748904506 1104 7 0.24423404083579214 1104 8 0.2096444797490733 1104 9 0.18009474763924516 1104 10-11 0.26157654689089327 1104 12-13 0.18803450479349948 1104 14-15 0.3661633182194066 1104 16-17 0.3910458629467257 1104 18-19 0.43948633646385105 1104 20-21 0.31747691588901006 1104 22-23 0.39414471198826107 1104 24-25 0.42324224207890637 1104 26-27 0.465107493766844 1104 28-29 0.47589958709450997 1104 30-31 0.5571039280378827 1104 32-33 0.41621665949436704 1104 34-35 0.40428613586691853 1104 36-37 0.5719131048181367 1104 38-39 0.4400176768991315 1104 40-41 0.27437674787572774 1104 42-43 0.38927055121747856 1104 44-45 0.5257467431027649 1104 46-47 0.45076046465042197 1104 48-49 0.409676228921505 1104 50-51 0.3198270485526109 1104 52-53 0.5135348010267577 1104 54-55 0.40303193332466947 1104 56-57 -2.736239875506122 1104 58-59 -9.971155462945088 1104 60-61 -10.194290758293207 1104 62-63 -3.779690211744562 1104 64-65 -5.2048989087249815 1104 66-67 -6.669321140442339 1104 68-69 -4.093397474941007 1104 70-71 -4.13224683576891 1104 72-73 -4.274238248106785 1104 74-75 -4.998397254451437 1104 76-77 -6.579718225484132 1104 78-79 -8.9599231493313 1104 80-81 -4.849163312458533 1104 82-83 -2.490194014917307 1104 84-85 -1.8937839730220993 1104 86-87 -1.8004770018371303 1104 88-89 -1.868278290029906 1104 90-91 -4.584271629549377 1104 92-93 -6.85279779721885 1104 94-95 -7.810587184517983 1104 96-97 -7.999240755902391 1104 98-99 -8.371630323173644 1104 100-101 -8.528681394166151 1105 1 0.2906432175140914 1105 2 0.3275832025554344 1105 3 0.28575301509776807 1105 4 0.2652702817950896 1105 5 0.22578071962134771 1105 6 0.2436502109398333 1105 7 0.28069841159401676 1105 8 0.2907501842773925 1105 9 0.3139730686825075 1105 10-11 0.382670542434532 1105 12-13 0.37391336775308304 1105 14-15 0.5634916277645132 1105 16-17 0.5864331515729972 1105 18-19 0.5054129177331461 1105 20-21 0.4655186575018959 1105 22-23 0.41960170011256537 1105 24-25 0.441838015053456 1105 26-27 0.4968377100140984 1105 28-29 0.6126085229746323 1105 30-31 0.6722631979500875 1105 32-33 0.6666933740758623 1105 34-35 0.6914544512528451 1105 36-37 0.4269163239104614 1105 38-39 0.4870000267467276 1105 40-41 0.5490637111569825 1105 42-43 0.5096637255704692 1105 44-45 0.5007107357298892 1105 46-47 0.5105720394054032 1105 48-49 0.4862318665106571 1105 50-51 0.6248174029885405 1105 52-53 0.6720706079140157 1105 54-55 0.4641547660009806 1105 56-57 1.832124743604389 1105 58-59 -8.363432803853797 1105 60-61 -9.710832676185074 1105 62-63 -3.2397576545593125 1105 64-65 -4.894841363301424 1105 66-67 -6.484772178419043 1105 68-69 -4.125679856296273 1105 70-71 -3.8840163604715876 1105 72-73 -2.1325486014026573 1105 74-75 -4.361921405062009 1105 76-77 -6.3809735348391 1105 78-79 -8.86947114504126 1105 80-81 -4.508557088463586 1105 82-83 -2.0039260107428305 1105 84-85 -1.4742018381450563 1105 86-87 -1.2813471211251013 1105 88-89 -1.3324184019504628 1105 90-91 -4.179652612737083 1105 92-93 -6.6150082887658535 1105 94-95 -7.956358284994658 1105 96-97 -8.306770464669215 1105 98-99 -8.517914252972833 1105 100-101 -8.225289165824162 1106 1 0.20394535602297736 1106 2 0.29287559253524975 1106 3 0.18977973042507656 1106 4 0.028485837973448724 1106 5 0.08731576152438691 1106 6 0.12926357492914065 1106 7 0.17030528124961108 1106 8 0.18259475252104806 1106 9 0.14280713722619254 1106 10-11 0.17764261732671116 1106 12-13 0.26401970016773646 1106 14-15 0.30876671884948337 1106 16-17 0.41578161843757755 1106 18-19 0.3235900236254068 1106 20-21 0.3268990724118126 1106 22-23 0.39317651805245646 1106 24-25 0.45456618870659327 1106 26-27 0.41814596858909425 1106 28-29 0.40231032130413524 1106 30-31 0.3861011277757953 1106 32-33 0.28822535858153486 1106 34-35 0.496551519226351 1106 36-37 0.31864206658126903 1106 38-39 0.5086495629096746 1106 40-41 0.44703207939414114 1106 42-43 0.32175986138919654 1106 44-45 0.2648457075236408 1106 46-47 0.30742335940157517 1106 48-49 0.2900462332911573 1106 50-51 0.318744691402145 1106 52-53 0.407985933821152 1106 54-55 0.23757680192890973 1106 56-57 1.7244832708877098 1106 58-59 -8.369074931978076 1106 60-61 -10.004211038773072 1106 62-63 -3.956958721485563 1106 64-65 -5.706628904055741 1106 66-67 -6.991034791281372 1106 68-69 -4.310483053912208 1106 70-71 -4.321602843963838 1106 72-73 -4.403083900724493 1106 74-75 -5.242528280867543 1106 76-77 -6.92155894736371 1106 78-79 -9.411963257746766 1106 80-81 -4.98641445911942 1106 82-83 -2.590938397594968 1106 84-85 -2.1503261564904115 1106 86-87 -2.125486406660535 1106 88-89 -1.9458485030817876 1106 90-91 -4.3247465442426005 1106 92-93 -6.622736343813063 1106 94-95 -7.890076238330692 1106 96-97 -7.487189635604963 1106 98-99 -6.941465042229421 1106 100-101 -7.335779157679887 1107 1 0.07994292016148918 1107 2 -0.04003613815309848 1107 3 -0.010453341213782608 1107 4 -0.005754225814577296 1107 5 -0.07968313445206121 1107 6 -0.09942395135713866 1107 7 0.08729891997104744 1107 8 0.10813931651937025 1107 9 0.04432341719254396 1107 10-11 0.06964458003524499 1107 12-13 0.01374522764565711 1107 14-15 0.06421542556474691 1107 16-17 0.0954590420321324 1107 18-19 0.11698209218000954 1107 20-21 0.22161158818357762 1107 22-23 0.18282629724774324 1107 24-25 0.14820271961925613 1107 26-27 0.09813882748436242 1107 28-29 0.05033293626278379 1107 30-31 0.0795887031297795 1107 32-33 0.15211590253218787 1107 34-35 0.2005089179365669 1107 36-37 0.22015834654762045 1107 38-39 0.05426785990952965 1107 40-41 -0.004588250410989758 1107 42-43 -0.016175176463036678 1107 44-45 0.040215467792812376 1107 46-47 -0.016700869534354013 1107 48-49 -0.010062416645752137 1107 50-51 -0.046955881638652386 1107 52-53 -0.0815094135081651 1107 54-55 -0.2842973218619207 1107 56-57 1.0213381180768337 1107 58-59 -8.876258532847274 1107 60-61 -10.693746691724144 1107 62-63 -4.330990948458787 1107 64-65 -5.814111730356036 1107 66-67 -7.311331081411751 1107 68-69 -4.079677730065299 1107 70-71 -4.120170680077674 1107 72-73 -2.491695591107222 1107 74-75 -5.015164706635101 1107 76-77 -7.016270367874014 1107 78-79 -7.663258513950327 1107 80-81 -2.7387633568482883 1107 82-83 -1.511897978066017 1107 84-85 -1.2949729565745258 1107 86-87 -1.347261080710723 1107 88-89 -1.041720462635972 1107 90-91 -0.5486504065726159 1107 92-93 -3.577636981693338 1107 94-95 -6.464019311021003 1107 96-97 -6.751091789327099 1107 98-99 -6.557823648355875 1107 100-101 -7.280404709691663 1108 1 -0.07771824135141614 1108 2 -0.09064388796142708 1108 3 -0.07657588146422967 1108 4 -0.02663414531368602 1108 5 -0.12756439563273858 1108 6 -0.2971199327013707 1108 7 -0.17013862794615164 1108 8 -0.07643054675688887 1108 9 -0.09673283759917695 1108 10-11 -0.08523019635115503 1108 12-13 -0.12769316680872578 1108 14-15 -0.17538414637493815 1108 16-17 -0.2702725737358094 1108 18-19 -0.13830876521840452 1108 20-21 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94-95 11.884820917248804 2316 96-97 13.64092348007241 2316 98-99 13.325620363845935 2316 100-101 12.19432237764039 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 16.0 4 30.0 5 48.0 6 145.0 7 385.0 8 920.0 9 1670.0 10 2746.0 11 4295.0 12 6542.0 13 9128.0 14 11650.0 15 16330.0 16 22325.0 17 30135.0 18 34017.0 19 35489.0 20 37127.0 21 37654.0 22 37413.0 23 31787.0 24 32498.0 25 37411.0 26 53573.0 27 80090.0 28 112639.0 29 154057.0 30 189654.0 31 206138.0 32 204903.0 33 186241.0 34 163498.0 35 146365.0 36 119192.0 37 75989.0 38 29095.0 39 2179.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37656424528124 19.755447643929497 14.077544915847865 23.790443194941403 2 15.757736361549945 21.079387763310752 39.52338476317115 23.639491111968145 3 19.226279506741122 25.053551833964 30.16362942083579 25.556539238459088 4 12.92409592604825 17.067604564824652 37.48950731956138 32.51879218956571 5 13.156713917959298 38.056867389500724 35.015060490720394 13.771358201819577 6 31.851992402684232 37.124783167043475 17.43871659494894 13.584507835323354 7 27.885404314798397 32.81750827348444 21.369911109134286 17.92717630258288 8 25.74952303079858 36.13311415462887 19.86813315768753 18.249229656885014 9 26.119416403501884 16.31023886876839 20.111404585455134 37.45894014227459 10-11 24.521831872954106 26.91021199236483 28.155162020234904 20.41279411444616 12-13 25.42590582470339 24.037370503525636 28.415361568067805 22.12136210370317 14-15 22.312008070552984 25.619226616168927 26.318467919835786 25.750297393442295 16-17 21.871950971383257 28.781481590136735 27.002528653179887 22.34403878530012 18-19 21.769667839199307 27.60216601510192 28.996831606710405 21.631334538988366 20-21 22.70343970647939 26.76605888771436 29.00051481561746 21.529986590188788 22-23 22.141469211636476 26.87296104825867 28.738652906031902 22.246916834072948 24-25 22.029140078111915 26.944422625767846 29.03318762663376 21.99324966948648 26-27 22.067438134471228 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28.16483720798038 22.12735116724951 52-53 22.337994343894348 27.452287412382574 27.96388484428423 22.24583339943884 54-55 22.287761703465637 27.442632763026985 27.970052879335956 22.299552654171414 56-57 21.421857964585133 27.541437029373427 28.91412116473399 22.122583841307446 58-59 20.99392912268164 26.966001692352936 30.072904871634382 21.967164313331043 60-61 22.096951741053548 27.341971288005716 28.52970194822555 22.031375022715185 62-63 22.259200118110545 27.36736282958035 28.193781735406354 22.17965531690275 64-65 21.73654220887665 27.416546605461907 28.53000077224504 22.31691041341641 66-67 21.88252350832501 27.01363458727149 29.181852393289816 21.921989511113686 68-69 22.19318190402091 26.37798489378205 29.766759351471162 21.66207385072589 70-71 22.057028598525697 26.372781327243068 29.640474842343206 21.929715231888032 72-73 21.219750174286833 26.62385741246523 30.014161716790944 22.142230696456995 74-75 21.71867166527727 26.85499038369585 29.17381892368876 22.252519027338124 76-77 19.893834253337246 27.47117915901864 29.781167583149642 22.853819004494472 78-79 20.757008390289393 27.437254375496266 29.10038248865099 22.70535474556335 80-81 21.666330131395522 27.47013140972455 28.000334246989112 22.863204211890814 82-83 22.02945765595038 27.486115202123617 27.759564392931203 22.724862748994795 84-85 22.12393023574383 27.37595726644757 27.580558959143303 22.919553538665298 86-87 21.923744648956905 27.279140975114192 27.813239783038775 22.983874592890132 88-89 22.040892903953583 27.23677872444726 27.69057914027522 23.03174923132394 90-91 21.352683406557905 27.426859636064155 28.151799493239476 23.06865746413846 92-93 20.424571678481573 27.47830924859954 28.82091111252062 23.276207960398267 94-95 19.796097237461247 27.323491855941175 29.52225852752673 23.35815237907085 96-97 19.887463043494076 27.23644160083821 29.23877480239335 23.637320553274364 98-99 20.077345224915565 27.250878339135305 28.87192515275646 23.799851283192673 100-101 20.47116068685531 27.2504621447434 28.48823125536621 23.790145913035087 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 229.0 1 190.5 2 157.5 3 177.0 4 198.5 5 237.0 6 307.0 7 448.5 8 633.0 9 773.0 10 876.0 11 1006.0 12 1177.0 13 1351.5 14 1529.0 15 1767.0 16 2043.5 17 2276.0 18 2580.5 19 2959.5 20 3395.0 21 4047.5 22 4967.5 23 6145.0 24 7692.0 25 9760.5 26 12438.0 27 15710.5 28 19091.0 29 22805.5 30 27067.5 31 31339.5 32 36147.5 33 41267.0 34 46182.5 35 50968.0 36 55994.5 37 60673.0 38 64641.5 39 67874.5 40 70580.0 41 73557.5 42 75678.0 43 77393.5 44 79269.0 45 81297.0 46 82614.5 47 83633.0 48 84892.5 49 84278.5 50 82103.0 51 78942.5 52 75325.0 53 71595.0 54 66660.5 55 60566.0 56 54085.5 57 47608.0 58 41046.5 59 34077.0 60 27097.5 61 20850.5 62 15499.5 63 11138.0 64 7880.5 65 5523.5 66 3841.0 67 2637.5 68 1762.5 69 1142.0 70 736.0 71 446.0 72 246.0 73 143.0 74 83.0 75 54.5 76 38.0 77 20.0 78 11.0 79 3.5 80 1.5 81 1.5 82 1.0 83 2.0 84 2.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02545687520169132 2 0.006766418501564794 3 2.365880594952725E-4 4 0.0 5 0.13670058077636843 6 0.0 7 0.0 8 0.0016087988045678527 9 0.0 10-11 0.0 12-13 0.0 14-15 7.57081790384872E-4 16-17 0.0 18-19 1.89270447596218E-4 20-21 0.0 22-23 3.0756447734385425E-4 24-25 0.0 26-27 1.89270447596218E-4 28-29 0.003122962385337597 30-31 0.011853061780713152 32-33 0.0026024686544479977 34-35 0.004211267459015851 36-37 0.10715073214540892 38-39 0.10674853244426695 40-41 0.11855427661308104 42-43 0.12122772168537764 44-45 0.117205724673958 46-47 0.11001344766530172 48-49 0.11060491781403989 50-51 0.11003710647125124 52-53 0.08775051126679656 54-55 0.0751876853075976 56-57 0.5705084466669001 58-59 1.60995808605938 60-61 0.5327963099833537 62-63 0.00489737283155214 64-65 0.12534435392059537 66-67 1.9262053451867105 68-69 2.907265051495757 70-71 2.810240288296746 72-73 2.669848933792251 74-75 2.031628984497804 76-77 1.4477769712753705 78-79 0.9578977352844593 80-81 0.05524331189214613 82-83 2.6024686544479976E-4 84-85 1.6561164164669075E-4 86-87 2.365880594952725E-4 88-89 1.89270447596218E-4 90-91 0.2592531955949196 92-93 0.4079487909876983 94-95 2.040642989564574 96-97 2.452613777563692 98-99 1.4619485960391374 100-101 0.9661783173667938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2113378.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.15801697492762 #Duplication Level Percentage of deduplicated Percentage of total 1 78.88312662289431 49.821018502841234 2 12.519712867427762 15.814404756044445 3 3.9210055309175833 7.4292880164143345 4 1.6979570252867782 4.289583945030398 5 0.8584646960809245 2.7109463923727555 6 0.5131308317686569 1.9444995469922135 7 0.34219114885466106 1.5128480071622867 8 0.23547602573162504 1.1897759064277174 9 0.1542121916260719 0.8765762594814215 >10 0.8064304525995794 8.97377542840212 >50 0.045708734561931426 1.9732961371636029 >100 0.021759313476652803 2.510968906222568 >500 5.247192195535306E-4 0.23927242721205402 >1k 2.2487966552289795E-4 0.4345630410326757 >5k 7.495988850763265E-5 0.2791827272000968 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5897 0.2790319573687244 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4713 0.22300790488024386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3312 0.15671593060966849 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.73176118990545E-5 2 0.0 0.0 0.0 0.0 4.73176118990545E-5 3 0.0 0.0 0.0 0.0 4.73176118990545E-5 4 0.0 0.0 0.0 4.73176118990545E-5 4.73176118990545E-5 5 0.0 0.0 0.0 4.73176118990545E-5 4.73176118990545E-5 6 0.0 0.0 0.0 4.73176118990545E-5 4.73176118990545E-5 7 0.0 0.0 0.0 4.73176118990545E-5 4.73176118990545E-5 8 0.0 0.0 0.0 4.73176118990545E-5 4.73176118990545E-5 9 0.0 0.0 0.0 9.4635223798109E-5 4.73176118990545E-5 10-11 0.0 0.0 0.0 9.4635223798109E-5 4.73176118990545E-5 12-13 0.0 0.0 0.0 9.4635223798109E-5 1.1829402974763626E-4 14-15 0.0 0.0 0.0 1.8927044759621802E-4 1.8927044759621802E-4 16-17 2.365880594952725E-5 0.0 0.0 3.0756447734385425E-4 2.83905671394327E-4 18-19 7.097641784858175E-5 0.0 0.0 4.73176118990545E-4 3.312232832933815E-4 20-21 9.4635223798109E-5 0.0 0.0 6.861053725362902E-4 3.5488208924290874E-4 22-23 9.4635223798109E-5 0.0 0.0 0.001135622685577308 4.0219970114196323E-4 24-25 9.4635223798109E-5 0.0 0.0 0.0015851399986183258 4.495173130410178E-4 26-27 9.4635223798109E-5 0.0 0.0 0.0019400220878612344 5.204937308895995E-4 28-29 9.4635223798109E-5 0.0 0.0 0.0033595504448328695 5.67811342788654E-4 30-31 9.4635223798109E-5 0.0 0.0 0.0089430286489213 5.67811342788654E-4 32-33 9.4635223798109E-5 0.0 0.0 0.02219195998065656 5.67811342788654E-4 34-35 1.1829402974763626E-4 0.0 0.0 0.03974679399520578 5.67811342788654E-4 36-37 1.419528356971635E-4 0.0 0.0 0.06695442083716212 5.67811342788654E-4 38-39 2.365880594952725E-4 0.0 0.0 0.1086175781142796 5.67811342788654E-4 40-41 2.365880594952725E-4 0.0 0.0 0.15352199180648232 5.67811342788654E-4 42-43 2.6024686544479976E-4 0.0 0.0 0.19953836937831282 5.67811342788654E-4 44-45 2.83905671394327E-4 0.0 0.0 0.2538589878384274 6.151289546877085E-4 46-47 3.0756447734385425E-4 0.0 0.0 0.3204348677803971 6.62446566586763E-4 48-49 3.78540895192436E-4 0.0 0.0 0.38407705578462537 6.62446566586763E-4 50-51 3.78540895192436E-4 0.0 0.0 0.44346065871793877 6.62446566586763E-4 52-53 4.0219970114196323E-4 0.0 0.0 0.502654991203656 6.62446566586763E-4 54-55 4.258585070914905E-4 0.0 0.0 0.5680952484600483 6.62446566586763E-4 56-57 4.258585070914905E-4 0.0 0.0 0.6221792788606676 6.62446566586763E-4 58-59 4.258585070914905E-4 0.0 0.0 0.6586611576348387 6.861053725362902E-4 60-61 4.258585070914905E-4 0.0 2.365880594952725E-5 0.6891336996978297 7.097641784858175E-4 62-63 6.151289546877085E-4 0.0 4.73176118990545E-5 0.7184233014633444 7.097641784858175E-4 64-65 6.62446566586763E-4 0.0 9.4635223798109E-5 0.7468848450206258 7.334229844353447E-4 66-67 6.62446566586763E-4 0.0 9.4635223798109E-5 0.7693843694786262 7.57081790384872E-4 68-69 6.62446566586763E-4 0.0 9.4635223798109E-5 0.802672309449611 7.57081790384872E-4 70-71 6.861053725362902E-4 0.0 9.4635223798109E-5 0.8542485064195804 7.57081790384872E-4 72-73 7.097641784858175E-4 0.0 9.4635223798109E-5 0.9305954732187048 7.57081790384872E-4 74-75 7.097641784858175E-4 0.0 9.4635223798109E-5 1.0309324692506499 7.57081790384872E-4 76-77 7.097641784858175E-4 0.0 9.4635223798109E-5 1.185164225235618 7.57081790384872E-4 78-79 7.097641784858175E-4 0.0 9.4635223798109E-5 1.426886245621938 7.807405963343992E-4 80-81 7.097641784858175E-4 0.0 9.4635223798109E-5 1.7227869316326752 8.043994022839265E-4 82-83 7.097641784858175E-4 0.0 9.4635223798109E-5 2.0277489403220814 8.51717014182981E-4 84-85 7.097641784858175E-4 2.365880594952725E-5 9.4635223798109E-5 2.256080076540969 8.990346260820354E-4 86-87 7.097641784858175E-4 4.73176118990545E-5 9.4635223798109E-5 2.4292152184796096 8.990346260820354E-4 88-89 7.097641784858175E-4 4.73176118990545E-5 9.4635223798109E-5 2.593975143112117 9.226934320315627E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4520 0.0 35.719994 1 GTATCAA 7045 0.0 34.075706 1 ATCAACG 8310 0.0 28.539833 3 TCAACGC 8460 0.0 28.033806 4 CAACGCA 8580 0.0 27.641726 5 TATCAAC 8675 0.0 27.505013 2 AACGCAG 8935 0.0 26.557135 6 TACATGG 7235 0.0 26.019253 2 GTACATG 7680 0.0 25.12918 1 ACATGGG 7335 0.0 24.827486 3 ACGCAGA 10480 0.0 22.417807 7 CGCAGAG 10475 0.0 22.340378 8 CATGGGG 2860 0.0 21.718498 4 CATGGGA 4810 0.0 21.229342 4 GCAGAGT 11335 0.0 19.691885 9 GAGTACT 5760 0.0 19.090117 12-13 AGAGTAC 9715 0.0 18.114412 10-11 AGTACTT 6220 0.0 17.867178 12-13 GTACTTT 6255 0.0 17.240711 14-15 GGACCGA 305 1.0507938E-7 16.94639 6 >>END_MODULE