FastQCFastQC Report
Fri 27 May 2016
SRR939618_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939618_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112666
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21790.10313982427889691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57350.042.9302141
GGTATCA36100.042.5184671
TATCAAC77950.031.2027472
GTACATG63950.028.8344061
ATCAACG85250.028.5862373
TCAACGC86950.027.9697024
CAACGCA88050.027.8136925
AACGCAG91000.027.1194556
TACATGG72000.025.4507712
ACGCAGA96300.025.2349017
ACATGGG73700.024.6714653
GTATTAG6051.8189894E-1223.8380761
CGCAGAG103350.023.2401148
CATGGGG31350.021.992044
GTATAGA8300.021.0993631
GAGTACT53050.020.32698412-13
GCAGAGT109200.020.2675749
CATGGGA48200.019.7903984
GTAGCCC13300.018.8202743
GTACTTT56450.018.77006114-15
Produced by FastQC (version 0.11.5)