FastQCFastQC Report
Fri 27 May 2016
SRR939618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112666
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58100.275007975704631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46730.2211897195297316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.15681607977787307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46550.038.0129241
GTATCAA72200.033.854181
ATCAACG85200.027.8473283
TCAACGC86650.027.3268974
TATCAAC88450.026.9904862
CAACGCA88250.026.8314515
AACGCAG91350.025.9037366
TACATGG73450.024.730162
GTACATG76900.024.4830931
ACATGGG73450.022.7335153
ACGCAGA104900.022.5577357
CGCAGAG105050.022.3909078
GCAGAGT113450.019.8189729
GAGTACT59600.018.98157312-13
CATGGGG33300.018.6975564
AGAGTAC99200.018.00921610-11
GTACTTT62650.017.53081314-15
AGTACTT63050.017.45697812-13
CATGGGA46500.017.3459594
TACACTG19650.017.2832375
Produced by FastQC (version 0.11.5)