##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939618_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2112666 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.450822799249856 31.0 31.0 34.0 30.0 34.0 2 31.653644258013333 31.0 31.0 34.0 30.0 34.0 3 31.711985235716387 31.0 31.0 34.0 30.0 34.0 4 35.519273278407475 37.0 35.0 37.0 33.0 37.0 5 35.26986897124297 37.0 35.0 37.0 33.0 37.0 6 35.26871403241213 37.0 35.0 37.0 32.0 37.0 7 35.171462029492595 37.0 35.0 37.0 32.0 37.0 8 35.12790190214639 37.0 35.0 37.0 32.0 37.0 9 36.732039044505854 39.0 35.0 39.0 32.0 39.0 10-11 36.60749782502298 39.0 35.0 39.0 32.0 39.0 12-13 36.54228472460862 38.0 35.0 39.0 32.0 39.0 14-15 37.623305103598966 39.0 36.0 41.0 32.0 41.0 16-17 37.57459153505571 39.0 36.0 41.0 32.0 41.0 18-19 37.52925048256563 39.0 36.0 41.0 32.0 41.0 20-21 37.462320120643774 39.0 36.0 41.0 32.0 41.0 22-23 37.334116230393256 39.0 36.0 40.5 31.5 41.0 24-25 37.21301663395917 39.0 36.0 40.5 31.0 41.0 26-27 36.932148763694784 39.0 36.0 40.0 31.0 41.0 28-29 36.735121642512354 39.0 35.5 40.0 30.0 41.0 30-31 36.49593073396363 38.0 35.0 40.0 30.0 41.0 32-33 36.33813816287099 38.0 35.0 40.0 30.0 41.0 34-35 36.32050120558574 38.0 35.0 40.0 30.0 41.0 36-37 36.26588514227994 38.0 35.0 40.0 30.0 41.0 38-39 36.32262056567389 38.0 35.0 40.0 30.0 41.0 40-41 36.21030276437449 38.0 35.0 40.0 30.0 41.0 42-43 36.04209823038758 38.0 35.0 40.0 29.5 41.0 44-45 35.84176959348993 38.0 35.0 40.0 28.5 41.0 46-47 35.5793568410719 38.0 34.0 40.0 27.5 41.0 48-49 35.309553426807646 38.0 34.0 40.0 27.0 41.0 50-51 35.08605406628402 38.0 34.0 40.0 26.5 41.0 52-53 34.852635248543784 38.0 33.5 40.0 26.0 41.0 54-55 34.57541442897268 38.0 33.0 40.0 25.5 41.0 56-57 33.831942910048255 37.5 32.5 40.0 23.0 41.0 58-59 30.719619665389608 35.5 27.0 39.0 8.5 40.5 60-61 30.46446646086035 34.5 26.0 39.0 10.5 40.0 62-63 32.23923232541254 35.5 30.5 39.0 19.5 40.0 64-65 32.29083821105655 36.0 31.0 39.0 18.5 40.0 66-67 27.39666705480185 32.0 19.0 37.0 4.5 39.5 68-69 19.71264246217812 19.5 8.0 32.0 2.0 37.0 70-71 17.771749296859987 15.5 7.5 29.0 2.0 35.0 72-73 19.492251023114868 20.0 7.5 30.5 2.0 35.5 74-75 20.99034300736605 23.5 9.5 32.5 2.0 36.0 76-77 22.936631725033678 27.5 12.0 33.0 2.0 35.5 78-79 25.2050638861041 30.5 14.5 34.5 2.0 37.0 80-81 26.824066842558167 32.0 23.0 35.0 2.0 37.0 82-83 27.88677907440173 33.0 26.5 35.0 2.0 36.5 84-85 27.910909012593564 34.0 27.0 35.0 2.0 36.0 86-87 27.620041218062866 33.5 27.0 35.0 2.0 36.0 88-89 27.170587305328908 33.0 26.0 35.0 2.0 35.5 90-91 25.378556288594602 32.5 16.0 34.5 2.0 35.0 92-93 23.80186314353523 31.5 3.5 34.0 2.0 35.0 94-95 19.924339436522384 28.0 2.0 34.0 2.0 35.0 96-97 17.18999430103954 18.5 2.0 33.0 2.0 35.0 98-99 17.4524584103687 21.5 2.0 33.0 2.0 35.0 100-101 16.663404437805124 10.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.287436633859528 1101 2 0.23605848215879632 1101 3 0.3466306230814915 1101 4 0.2255114112410581 1101 5 0.35083161422708997 1101 6 0.347617441081006 1101 7 0.27286534505831384 1101 8 0.28596555452065786 1101 9 0.2806754396890625 1101 10-11 0.39888303928366753 1101 12-13 0.3010888663441307 1101 14-15 0.48967986826693277 1101 16-17 0.42980730189733407 1101 18-19 0.3769141312919757 1101 20-21 0.43907218556518757 1101 22-23 0.5318243444760569 1101 24-25 0.43510426146427506 1101 26-27 0.4767763185937355 1101 28-29 0.40379302614989854 1101 30-31 0.28471828392594745 1101 32-33 0.36399284344668814 1101 34-35 0.30192901389668947 1101 36-37 0.21257756902780045 1101 38-39 0.08173924444824365 1101 40-41 0.17690026674113568 1101 42-43 0.2793492892019742 1101 44-45 0.34326527936310924 1101 46-47 6.023317375607462E-4 1101 48-49 -0.025085007414432425 1101 50-51 0.06742586688429242 1101 52-53 0.027868834816025867 1101 54-55 7.5225729425199E-5 1101 56-57 -3.5735778814573855 1101 58-59 -10.62268706040176 1101 60-61 -12.207857771403429 1101 62-63 -4.3793992557024986 1101 64-65 -1.317425652938951 1101 66-67 -2.0667727520352983 1101 68-69 -4.412684026379381 1101 70-71 -4.533633854668404 1101 72-73 -3.635236781104407 1101 74-75 -6.318314893332454 1101 76-77 -4.806922802396453 1101 78-79 1.227001157067221 1101 80-81 1.9241900426753524 1101 82-83 0.35565477255642364 1101 84-85 0.23558855502470877 1101 86-87 0.20218966768177182 1101 88-89 0.014111337487982212 1101 90-91 1.3129411909663737 1101 92-93 0.8877593417390166 1101 94-95 -8.849313668192568 1101 96-97 -9.70542637979433 1101 98-99 -8.797396285182707 1101 100-101 -9.644831359581591 1102 1 0.255060730067207 1102 2 0.2681483584420121 1102 3 0.281280586796111 1102 4 0.24289261944084473 1102 5 0.31956649139343796 1102 6 0.2323839435878412 1102 7 0.30661602623310813 1102 8 0.3324411646028338 1102 9 0.22898429968773115 1102 10-11 0.3368667581233282 1102 12-13 0.34296158014828393 1102 14-15 0.39714300197603336 1102 16-17 0.24400814404969395 1102 18-19 0.19862098303482156 1102 20-21 0.17706496162880825 1102 22-23 0.2691548698816604 1102 24-25 0.13577185983871942 1102 26-27 0.03813524489279274 1102 28-29 0.13439738589454464 1102 30-31 0.31588115992784793 1102 32-33 0.19700305419566888 1102 34-35 0.06567019722582046 1102 36-37 -0.027799460044569457 1102 38-39 0.047073802483964755 1102 40-41 -0.115264688467974 1102 42-43 -0.00433096781982556 1102 44-45 -0.2739319033694869 1102 46-47 -0.2263259437105134 1102 48-49 -0.0024249063877874732 1102 50-51 -0.04743272555909073 1102 52-53 -0.1648308543762056 1102 54-55 -0.35373440083802876 1102 56-57 -3.753304462507863 1102 58-59 -10.327497256011434 1102 60-61 -8.57014362266385 1102 62-63 -2.7983481168460287 1102 64-65 -1.113478341503388 1102 66-67 -2.164761652887506 1102 68-69 -5.051143089421803 1102 70-71 -5.039234081230813 1102 72-73 -3.682890960100007 1102 74-75 -4.262341531739079 1102 76-77 -4.379414864615249 1102 78-79 1.6836600808430546 1102 80-81 2.0155325228633814 1102 82-83 0.6908806137766952 1102 84-85 0.33688004737276955 1102 86-87 0.19652250051403897 1102 88-89 0.2113461350034811 1102 90-91 1.3683695185735054 1102 92-93 0.9885965606402571 1102 94-95 -8.937900756319547 1102 96-97 -9.776867446264562 1102 98-99 -8.899064753879172 1102 100-101 -9.526561055865193 1103 1 0.22639440477126627 1103 2 0.21915443479199936 1103 3 0.1502943241517407 1103 4 0.16911668695878035 1103 5 0.24352083538492764 1103 6 0.277137242015165 1103 7 0.21735645787362046 1103 8 0.2821617744564975 1103 9 0.45298257618186 1103 10-11 0.3106292096451355 1103 12-13 0.25689841358458665 1103 14-15 0.42929536768252774 1103 16-17 0.36860031745231225 1103 18-19 0.21341808172647347 1103 20-21 0.42759823094900895 1103 22-23 0.33023785382425785 1103 24-25 0.3722560033642992 1103 26-27 0.3857624673227562 1103 28-29 0.40389904585256886 1103 30-31 0.26868751383134537 1103 32-33 0.33725334456894274 1103 34-35 0.3546043675296531 1103 36-37 0.4345425611003364 1103 38-39 0.2689050238001869 1103 40-41 0.30034266368776485 1103 42-43 0.286158931010533 1103 44-45 0.31729636271454353 1103 46-47 0.27187331957521366 1103 48-49 0.2306716407966789 1103 50-51 0.1377237637784674 1103 52-53 0.11815938790640246 1103 54-55 0.12055535091394631 1103 56-57 0.4913074695010806 1103 58-59 -8.572074842794656 1103 60-61 -7.785681035450857 1103 62-63 -2.3965099860785095 1103 64-65 -0.574063705368097 1103 66-67 -2.018572944422271 1103 68-69 -3.7089252400426993 1103 70-71 -4.2763249598000534 1103 72-73 -3.3194953302820345 1103 74-75 -3.8900937414043746 1103 76-77 -0.45035988727575216 1103 78-79 3.278478525000658 1103 80-81 2.7905333677192026 1103 82-83 1.1585312680991606 1103 84-85 0.6461576111612182 1103 86-87 0.6155331523092862 1103 88-89 0.4853944049224239 1103 90-91 1.6792237486196662 1103 92-93 1.157056035845212 1103 94-95 -7.4102068417058184 1103 96-97 -9.005684890553667 1103 98-99 -8.370056537472658 1103 100-101 -9.656496666831863 1104 1 0.18764332132573003 1104 2 0.22651165872206036 1104 3 0.23609318708977511 1104 4 0.17723077368253826 1104 5 0.25564831783292874 1104 6 0.2502351709119779 1104 7 0.21261936791538005 1104 8 0.252757481788791 1104 9 0.3467584412798601 1104 10-11 0.28327025074425904 1104 12-13 0.2069349901226758 1104 14-15 0.4338971408518546 1104 16-17 0.2903008294144982 1104 18-19 0.35621857247536326 1104 20-21 0.32588529353406415 1104 22-23 0.24651193474533528 1104 24-25 0.3520718413151087 1104 26-27 0.3155091798082026 1104 28-29 0.28423781712874074 1104 30-31 0.3055439799873838 1104 32-33 0.3558574439113329 1104 34-35 0.37030375759264444 1104 36-37 0.31625855658358404 1104 38-39 0.12472676210973077 1104 40-41 0.09279386398869605 1104 42-43 0.11492952194303996 1104 44-45 0.21626711679263622 1104 46-47 0.12558602954860731 1104 48-49 0.1857975865562338 1104 50-51 0.24998740134160613 1104 52-53 0.2690849911914768 1104 54-55 0.24599538106452457 1104 56-57 0.6445153248365898 1104 58-59 -8.292628178203152 1104 60-61 -7.944391135678224 1104 62-63 -2.4956893558175572 1104 64-65 -0.625266940432585 1104 66-67 -2.039871898118232 1104 68-69 -3.5370142352977734 1104 70-71 -4.088368664298073 1104 72-73 -3.01948862367216 1104 74-75 -3.6511752296302653 1104 76-77 -0.21353190598719252 1104 78-79 3.453113452150106 1104 80-81 2.7916427831433843 1104 82-83 1.2135143521337106 1104 84-85 0.858375614618037 1104 86-87 0.8488521004504257 1104 88-89 0.4984921588642024 1104 90-91 1.8674030206754821 1104 92-93 1.7016305707017203 1104 94-95 -6.754174373695253 1104 96-97 -8.165118867259862 1104 98-99 -6.0595467877385545 1104 100-101 -5.201507951431955 1105 1 0.28319747043234855 1105 2 0.2611023686499614 1105 3 0.29405591727578795 1105 4 0.20556124486937932 1105 5 0.3305104878153813 1105 6 0.25528732957265987 1105 7 0.3227265152102561 1105 8 0.22442588610916658 1105 9 0.3720096763946188 1105 10-11 0.3557455910860554 1105 12-13 0.2906340803517935 1105 14-15 0.4324985529524099 1105 16-17 0.49231520830372233 1105 18-19 0.5063251462789182 1105 20-21 0.3694348374679137 1105 22-23 0.49765062425020545 1105 24-25 0.6364204573174987 1105 26-27 0.49841271013455213 1105 28-29 0.5072921119508891 1105 30-31 0.494296840650172 1105 32-33 0.32932185062237807 1105 34-35 0.43094418621931396 1105 36-37 0.3830298135734722 1105 38-39 0.43028649807132524 1105 40-41 0.3797795092760623 1105 42-43 0.2861013127671441 1105 44-45 0.43385047309998015 1105 46-47 0.4110216552416901 1105 48-49 0.44860560621387435 1105 50-51 0.34888063806101854 1105 52-53 0.3669650599129781 1105 54-55 0.3968981894665191 1105 56-57 1.0646196440321063 1105 58-59 -8.150262706428762 1105 60-61 -7.2070452872399855 1105 62-63 -1.697539277637194 1105 64-65 0.01310667566820456 1105 66-67 -1.4950483975684499 1105 68-69 -3.372330393003388 1105 70-71 -3.5980466114390417 1105 72-73 -2.807637088205375 1105 74-75 -3.4812931383210746 1105 76-77 0.15999470391632897 1105 78-79 3.937209282174777 1105 80-81 3.4303631178524085 1105 82-83 1.8279561379856837 1105 84-85 1.5041950596845979 1105 86-87 1.4600776495261947 1105 88-89 1.464043603565674 1105 90-91 2.956104604467342 1105 92-93 2.856676385348951 1105 94-95 -6.156969937721154 1105 96-97 -7.802286933389034 1105 98-99 -5.62516075423879 1105 100-101 -5.163206227903125 1106 1 0.10817321101111332 1106 2 0.12155479296254512 1106 3 0.14831533662835383 1106 4 0.14637032929969962 1106 5 0.2839075320750055 1106 6 0.309577945664941 1106 7 0.20779309180324645 1106 8 0.18748600746373256 1106 9 0.1336449205722161 1106 10-11 0.2469766321434932 1106 12-13 0.20315350531449639 1106 14-15 0.2548038262375485 1106 16-17 0.30872051122198485 1106 18-19 0.14381516887959123 1106 20-21 0.08277673734558988 1106 22-23 0.2144526117990111 1106 24-25 0.15767700397028506 1106 26-27 0.23774737184113803 1106 28-29 0.34400440133757115 1106 30-31 0.4539799526078454 1106 32-33 0.3917191919668923 1106 34-35 0.2575118836154573 1106 36-37 0.37722300013769683 1106 38-39 0.3315442014148289 1106 40-41 0.2735381267631496 1106 42-43 0.21322876181011452 1106 44-45 0.2944285509475293 1106 46-47 0.17298393770517606 1106 48-49 0.23277437228674103 1106 50-51 0.1683911092203303 1106 52-53 0.19455484614560703 1106 54-55 0.23089668275441255 1106 56-57 0.6240545196134519 1106 58-59 -8.442630318561356 1106 60-61 -7.534626784483802 1106 62-63 -1.8404438937939034 1106 64-65 -0.22554216640715197 1106 66-67 0.6069069183767191 1106 68-69 -2.4761314218552855 1106 70-71 -2.5461301312903224 1106 72-73 -1.0449159864932653 1106 74-75 -1.0602977513317349 1106 76-77 2.3562837989791596 1106 78-79 4.497508002260712 1106 80-81 3.549061626041645 1106 82-83 1.9018163031211763 1106 84-85 1.508806845835469 1106 86-87 1.251877936890363 1106 88-89 1.233107477194764 1106 90-91 2.707924838152774 1106 92-93 2.3404389156155325 1106 94-95 -6.140332241698346 1106 96-97 -7.654760923389876 1106 98-99 -5.6447506489818196 1106 100-101 -5.064749995553726 1107 1 0.04776864724156127 1107 2 -0.008066844211960245 1107 3 0.08841208989684901 1107 4 0.07832348623566077 1107 5 -0.004869662764576788 1107 6 0.014001420033018519 1107 7 0.04645363154784121 1107 8 0.09813181051107733 1107 9 0.06043166048820581 1107 10-11 -0.03981267966687341 1107 12-13 0.142532777317534 1107 14-15 0.10547665809195195 1107 16-17 0.13700682377669438 1107 18-19 0.09859416441317848 1107 20-21 0.13864470386754846 1107 22-23 0.1532782274255311 1107 24-25 0.11143239289847884 1107 26-27 0.1762458895984338 1107 28-29 0.14989048886071998 1107 30-31 0.2061995452918879 1107 32-33 0.11409634721622552 1107 34-35 0.05262682963326881 1107 36-37 0.12475072526952857 1107 38-39 0.19843974460517444 1107 40-41 0.13633124085628623 1107 42-43 0.10887635488123237 1107 44-45 0.07664290785803018 1107 46-47 0.17609909418416692 1107 48-49 0.01956184440155795 1107 50-51 0.24197718360804998 1107 52-53 0.16654051418137783 1107 54-55 0.031933240816492514 1107 56-57 0.7597977437387726 1107 58-59 -8.663154808404585 1107 60-61 -8.099396393890679 1107 62-63 -2.1694234956175578 1107 64-65 -0.10277330318078981 1107 66-67 0.9295806877399748 1107 68-69 -2.7207529799228816 1107 70-71 -2.448848686202105 1107 72-73 -0.9258519941552308 1107 74-75 -0.8484639832783429 1107 76-77 2.3420246866911363 1107 78-79 4.392987815097445 1107 80-81 3.3589214559581144 1107 82-83 1.7198895458163896 1107 84-85 1.3045131619886199 1107 86-87 1.2055129177446524 1107 88-89 1.2297274965410026 1107 90-91 2.440246375726165 1107 92-93 2.0035266385544404 1107 94-95 -6.618504713892266 1107 96-97 -7.99275061815127 1107 98-99 -5.84296910620577 1107 100-101 -5.445212097641374 1108 1 -0.10997290017341754 1108 2 -0.07173565220625022 1108 3 -0.10044187082904799 1108 4 -0.15692262039989657 1108 5 -0.10376851287347222 1108 6 -0.06678778606931246 1108 7 -0.14815526125981648 1108 8 -0.1808112535407247 1108 9 -0.16613577125127676 1108 10-11 -0.18202047424546208 1108 12-13 -0.08433659439540264 1108 14-15 -0.3106512081928159 1108 16-17 -0.2835061090754252 1108 18-19 -0.2831521585457537 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2313 1 -0.1906275059437803 2313 2 -0.2861523434308211 2313 3 -0.27069338330704795 2313 4 -0.252144823056895 2313 5 -0.30963284923069523 2313 6 -0.19320147742800486 2313 7 -0.15347619125473244 2313 8 -0.23516283818702988 2313 9 -0.2799241146556284 2313 10-11 -0.26801288881816987 2313 12-13 -0.32836856741850795 2313 14-15 -0.4811267982439915 2313 16-17 -0.3912149797554818 2313 18-19 -0.3929471585868143 2313 20-21 -0.33456473329462 2313 22-23 -0.36014670313282693 2313 24-25 -0.39958164944248153 2313 26-27 -0.371746395995153 2313 28-29 -0.24781108079105962 2313 30-31 -0.5271740527533169 2313 32-33 -0.5109459243746173 2313 34-35 -0.4628840745301801 2313 36-37 -0.4576578999875096 2313 38-39 -0.5318748613691184 2313 40-41 -0.3795864876400472 2313 42-43 -0.40611394126871403 2313 44-45 -0.36496426983084973 2313 46-47 -0.5967111284395514 2313 48-49 -0.6983173767187338 2313 50-51 -0.7513598874238383 2313 52-53 -0.45874648767221515 2313 54-55 -0.4943218213313756 2313 56-57 0.38199360713442587 2313 58-59 3.2440494693532784 2313 60-61 3.284034086913934 2313 62-63 0.41118938160758134 2313 64-65 -0.3275436562934182 2313 66-67 -2.1625305162384727 2313 68-69 -4.8109163307896186 2313 70-71 -3.495079367948879 2313 72-73 -3.108699190572562 2313 74-75 -3.8727750062926134 2313 76-77 0.3969732143321245 2313 78-79 2.8341133418350637 2313 80-81 2.287633755238435 2313 82-83 0.6665635934388128 2313 84-85 0.3639896989400775 2313 86-87 0.36505734350665975 2313 88-89 0.48489797954682246 2313 90-91 2.09099331609843 2313 92-93 3.5637190228755777 2313 94-95 5.75225582240391 2313 96-97 1.0294916849684554 2313 98-99 2.74855973963723 2313 100-101 3.7116568224688606 2314 1 -0.33163924239650555 2314 2 -0.24990833605345486 2314 3 -0.32653911405691005 2314 4 -0.12564108074041513 2314 5 -0.2661487183211264 2314 6 -0.361566053840086 2314 7 -0.34705726931599656 2314 8 -0.46608700562589434 2314 9 -0.5430341405479311 2314 10-11 -0.4587455157992011 2314 12-13 -0.6002921992830181 2314 14-15 -0.6934822155152602 2314 16-17 -0.5475555767383824 2314 18-19 -0.5320639934988165 2314 20-21 -0.4627067632927435 2314 22-23 -0.5213362405872743 2314 24-25 -0.5473099463905271 2314 26-27 -0.7675924544956629 2314 28-29 -0.6772406866560061 2314 30-31 -0.643138563317315 2314 32-33 -0.8260241673927808 2314 34-35 -0.6377414657542104 2314 36-37 -0.5883364592850526 2314 38-39 -0.6392761413843715 2314 40-41 -0.5794394281592616 2314 42-43 -0.7186721356308112 2314 44-45 -0.7038559251503926 2314 46-47 -0.6835755715167195 2314 48-49 -0.619412432600214 2314 50-51 -0.6564047520181333 2314 52-53 -0.5053045844540023 2314 54-55 -0.36098783001378365 2314 56-57 0.27114647599543673 2314 58-59 3.4545945298530683 2314 60-61 3.477807439147554 2314 62-63 0.904217883250805 2314 64-65 -0.0035210794880313756 2314 66-67 -1.707556658077035 2314 68-69 -4.786060541074434 2314 70-71 -3.7271713332907375 2314 72-73 -2.9607045031125985 2314 74-75 -4.177195487281587 2314 76-77 0.21552850065210905 2314 78-79 3.2799399773852826 2314 80-81 2.6161808738633106 2314 82-83 0.9847709240289682 2314 84-85 0.6702023800595533 2314 86-87 0.5610004608920427 2314 88-89 0.7461885237916732 2314 90-91 2.4885077371269126 2314 92-93 4.135192500399331 2314 94-95 6.474961735472519 2314 96-97 3.4022424961513096 2314 98-99 6.18851880060183 2314 100-101 6.452760533946986 2315 1 -0.3679588064816066 2315 2 -0.36226105605271286 2315 3 -0.4522745509426329 2315 4 -0.30558908007470365 2315 5 -0.3202075671408835 2315 6 -0.4066682907393684 2315 7 -0.41140549271005966 2315 8 -0.640662540271947 2315 9 -0.5989974588514855 2315 10-11 -0.3711861492072188 2315 12-13 -0.5609400656299783 2315 14-15 -0.6415969313415246 2315 16-17 -0.642095815036889 2315 18-19 -0.6751579945836639 2315 20-21 -0.6357333152995679 2315 22-23 -0.5335265211088682 2315 24-25 -0.3925575948416835 2315 26-27 -0.40457479784645756 2315 28-29 -0.5359931753528571 2315 30-31 -0.4440678565890508 2315 32-33 -0.32441653086888067 2315 34-35 -0.3911302926611029 2315 36-37 -0.38811646387720344 2315 38-39 -0.4881070702615773 2315 40-41 -0.4616696511095171 2315 42-43 -0.2919472105999148 2315 44-45 -0.3701144545231756 2315 46-47 -0.39464104581764303 2315 48-49 -0.4064342060009025 2315 50-51 -0.38918978870907495 2315 52-53 -0.3472357361581828 2315 54-55 -0.19689506403142332 2315 56-57 0.3399589438272912 2315 58-59 3.5741524317352287 2315 60-61 3.847420238754289 2315 62-63 1.0208459802529681 2315 64-65 0.17698581720716788 2315 66-67 -1.6692537451459835 2315 68-69 -4.52254645489618 2315 70-71 -3.5906457004837034 2315 72-73 -3.2557495308041666 2315 74-75 -0.37800837811485977 2315 76-77 2.254213771419245 2315 78-79 3.8593765032877627 2315 80-81 2.774100824120193 2315 82-83 0.9973301750467947 2315 84-85 0.756236264090905 2315 86-87 0.7988540022383113 2315 88-89 0.9967374047256357 2315 90-91 2.5806887216338126 2315 92-93 4.090830515975643 2315 94-95 6.896711963538856 2315 96-97 4.455197033900909 2315 98-99 6.741869372462759 2315 100-101 6.721295450440806 2316 1 -0.5355153391135623 2316 2 -0.43413541391442223 2316 3 -0.5608602980835791 2316 4 -0.349556919093267 2316 5 -0.4671222180882353 2316 6 -0.6061060347019946 2316 7 -0.5327462405169783 2316 8 -0.5725917378669223 2316 9 -0.6761856514747038 2316 10-11 -0.6812087684504888 2316 12-13 -0.49609051345186117 2316 14-15 -0.5803387606466117 2316 16-17 -0.9357690567336974 2316 18-19 -0.7697789999468299 2316 20-21 -0.620968203370559 2316 22-23 -0.8844855374843092 2316 24-25 -0.6692001059590993 2316 26-27 -0.8816755847772555 2316 28-29 -0.803042826858352 2316 30-31 -0.8181914782320305 2316 32-33 -0.7042473827818299 2316 34-35 -0.6688644263912167 2316 36-37 -0.5689905327829194 2316 38-39 -0.6714175953087818 2316 40-41 -0.590950897546243 2316 42-43 -0.5631538744402604 2316 44-45 -0.6846464446671234 2316 46-47 -0.7689859274724924 2316 48-49 -0.94559812672032 2316 50-51 -0.780300707670996 2316 52-53 -0.6183464474392295 2316 54-55 -0.4106278422444163 2316 56-57 0.374966251598174 2316 58-59 3.478225732700963 2316 60-61 3.5013394265303504 2316 62-63 0.9508389530509582 2316 64-65 0.33402171584531715 2316 66-67 -1.858269603378222 2316 68-69 -3.0896879852206816 2316 70-71 -2.9737811745143077 2316 72-73 -0.2129886371932912 2316 74-75 5.886723135441411 2316 76-77 6.046364053296113 2316 78-79 5.07051207237231 2316 80-81 3.362954291881632 2316 82-83 1.6436255775313349 2316 84-85 1.305777738661078 2316 86-87 1.307616837266007 2316 88-89 1.246704305585773 2316 90-91 2.5996815372733018 2316 92-93 4.339846478048326 2316 94-95 6.890184893035396 2316 96-97 4.078534713222865 2316 98-99 6.332957506436095 2316 100-101 6.415993876838801 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 29.0 5 71.0 6 152.0 7 453.0 8 922.0 9 1668.0 10 2921.0 11 4451.0 12 6586.0 13 9026.0 14 11090.0 15 15129.0 16 19922.0 17 26494.0 18 29111.0 19 29522.0 20 31557.0 21 32707.0 22 32684.0 23 33094.0 24 34952.0 25 37886.0 26 47224.0 27 66500.0 28 93036.0 29 123251.0 30 148549.0 31 167185.0 32 175946.0 33 175591.0 34 177737.0 35 188805.0 36 188190.0 37 141260.0 38 55231.0 39 3727.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.17375433386849 19.80732365185501 14.168027448899057 23.85089456537744 2 15.692153875722752 21.128940175326143 39.49946175119745 23.679444197753654 3 19.20068690525327 24.99252129065483 30.251237300394195 25.555554503697703 4 12.887792012556645 17.078752628195843 37.53238798750015 32.501067371747354 5 13.143644142851324 38.107863112338705 34.99228493729913 13.756207807510842 6 31.819558794433195 37.25420866336657 17.384622084134456 13.541610458065781 7 27.825837117651346 32.837419639450815 21.40745389948056 17.929289343417274 8 25.710016221233577 36.14878473282298 19.847509990945078 18.29368905499836 9 26.052390676046283 16.340869782540164 20.080457582978095 37.526281958435455 10-11 24.544367164521034 26.897862700493118 28.195157208948313 20.362612926037528 12-13 25.381555816205687 24.09611836419008 28.428984988635214 22.093340830969023 14-15 22.295970832943816 25.633757483169894 26.349017765539372 25.721253918346914 16-17 21.888865038028975 28.72813987827226 27.09553405401849 22.287461029680276 18-19 21.801741547332597 27.607569882823157 29.048146141726917 21.542542428117333 20-21 22.685317982113595 26.87362791846889 28.929490037705914 21.5115640617116 22-23 22.079685099301074 26.906075072917346 28.803511771382702 22.21072805639888 24-25 22.057545869252664 26.93598717258436 29.018619869477497 21.987847088685484 26-27 22.03128091478902 27.139362567225618 28.80361364004275 22.025742877942616 28-29 21.940440050372885 27.298322079270793 28.859919404329503 21.90131846602682 30-31 22.194509466070546 27.101742211408514 28.75609750324845 21.94765081927249 32-33 22.01751437835791 27.05722941468841 28.728976828950792 22.196279378002888 34-35 22.14663172752773 27.19785707456029 28.78197560760873 21.873535590303252 36-37 22.279698981691 26.89635210359176 28.803286178914554 22.02066273580269 38-39 22.070476307323254 27.18868566710476 28.79890138494563 21.94193664062635 40-41 22.277540689234044 27.056809899623786 28.80991762551527 21.8557317856269 42-43 22.05419231114519 27.103853949705677 28.619013001729886 22.222940737419254 44-45 22.140953981809517 27.23849166230018 28.5630057978732 22.057548558017107 46-47 22.295762130531838 27.227657574689513 28.134510287691533 22.34207000708712 48-49 22.1757819651642 27.310581739023544 28.266658392842892 22.246977902969363 50-51 22.245845202716595 27.45541106512301 28.15187189686876 22.14687183529164 52-53 22.335531098751673 27.455743546777544 28.006092677467464 22.202632677003315 54-55 22.30957456027982 27.424800876291126 27.995735042221 22.269889521208057 56-57 22.156631396664608 27.591952016756053 28.082517500403792 22.168899086175546 58-59 21.35289714100437 27.051529629417708 29.602978199919892 21.992595029658027 60-61 21.528864266559513 27.338955051899728 29.09937560736504 22.03280507417572 62-63 22.24121926963295 27.3430152006358 28.202521148021237 22.21324438171001 64-65 22.108685434120236 27.411178204975883 28.15080167885606 22.32933468204782 66-67 22.152150649971077 27.123753832176227 28.788502166889373 21.935593350963316 68-69 21.693824709069474 26.405241660217982 30.295818268547787 21.60511536216476 70-71 22.500211493668886 26.35116578461485 29.34858253185919 21.80004018985708 72-73 21.6830818101951 26.636435350243843 29.57674389000809 22.103738949552966 74-75 21.722073619973166 26.90480483446967 29.072729958117506 22.300391587439663 76-77 20.60224186483477 27.404282004754606 29.31144085477952 22.682035275631105 78-79 21.89929906820102 27.157025383489426 28.434339707964916 22.509335840344637 80-81 21.79641591894727 27.429141961364433 27.936739589087185 22.837702530601113 82-83 22.085065152369307 27.46334839015972 27.73757575155148 22.714010705919495 84-85 22.09291592845164 27.39651459949892 27.59820527955294 22.9123641924965 86-87 21.92059023864488 27.262468658837275 27.83466823690014 22.98227286561771 88-89 22.050035843345764 27.28299865738329 27.672719604688957 22.994245894581987 90-91 21.83105999425838 27.222985107161524 28.069696365989742 22.876258532590356 92-93 21.644430983820335 27.30332366646878 27.90727576990573 23.14496957980515 94-95 20.710091836517663 27.20883565328339 28.8743866950922 23.20668581510675 96-97 20.573000468619014 27.031470642956357 28.98349628293902 23.41203260548561 98-99 21.112637225210136 26.985683271137432 28.402492028396203 23.499187475256228 100-101 20.468568049793717 27.06935852599326 28.850953056073152 23.61112036813987 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 318.0 1 245.0 2 161.5 3 196.5 4 243.5 5 271.0 6 346.5 7 521.5 8 763.0 9 964.0 10 1112.0 11 1209.5 12 1334.5 13 1432.5 14 1592.0 15 1805.0 16 1956.0 17 2180.5 18 2449.0 19 2799.5 20 3246.0 21 3874.0 22 4796.0 23 5904.0 24 7363.5 25 9392.0 26 12141.5 27 15544.0 28 19098.0 29 22735.0 30 26532.5 31 30917.0 32 35498.5 33 40152.0 34 45067.5 35 49976.0 36 55139.0 37 59797.5 38 63508.5 39 66536.5 40 69308.0 41 71999.5 42 74081.0 43 75928.0 44 78251.0 45 80255.5 46 81646.0 47 83080.5 48 84224.5 49 83410.0 50 81241.5 51 78686.5 52 75795.5 53 72606.0 54 67991.0 55 62107.5 56 55884.5 57 49384.0 58 42836.5 59 36095.5 60 29143.0 61 22854.0 62 17222.0 63 12345.0 64 8613.5 65 6067.5 66 4233.0 67 2917.0 68 2036.5 69 1366.5 70 864.0 71 498.5 72 300.0 73 195.5 74 108.5 75 61.0 76 33.5 77 16.0 78 8.5 79 4.5 80 1.5 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0247554511692809 2 0.012306725246678842 3 9.466711728214493E-5 4 0.0 5 0.05736827307297982 6 0.0 7 0.0 8 1.420006759232174E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 4.023352484491159E-4 16-17 2.366677932053623E-5 18-19 2.1300101388482608E-4 20-21 0.0 22-23 0.0 24-25 1.6566745524375362E-4 26-27 1.8933423456428985E-4 28-29 0.001017671510783058 30-31 0.005727360595569768 32-33 0.005490692802364406 34-35 0.0018933423456428985 36-37 0.08352006422217237 38-39 0.07784003718524367 40-41 0.08318872931168486 42-43 0.08669141265112422 44-45 0.0895550929489091 46-47 0.084916404202084 48-49 0.07528402501862576 50-51 0.07062166949248011 52-53 0.06053962150193168 54-55 0.04917956742807429 56-57 0.06763965529809254 58-59 0.7301674755971839 60-61 0.3436416357341861 62-63 0.0026980128425411307 64-65 0.0017513416697196812 66-67 1.1176163198536826 68-69 2.4081657962025234 70-71 2.3646189222527365 72-73 1.8350274013970973 74-75 1.2925848193704068 76-77 0.7455508821555324 78-79 0.5628433458009926 80-81 0.040493859417437494 82-83 1.420006759232174E-4 84-85 0.0011833389660268116 86-87 0.0013726732005911015 88-89 2.366677932053623E-5 90-91 0.330837908121776 92-93 0.05478859412704137 94-95 0.9663382664368149 96-97 1.6701882834295623 98-99 0.6462450761265623 100-101 0.5755760730754411 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2112666.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.38178229592181 #Duplication Level Percentage of deduplicated Percentage of total 1 78.91292406560588 50.01641773460841 2 12.661140145445845 16.049712566336087 3 3.886078983551269 7.389198363606109 4 1.6079926566778753 4.076697619959922 5 0.8542505688952713 2.707196179194372 6 0.5145118715503937 1.9566407658764602 7 0.32053939451852176 1.4221450684447676 8 0.21647829505078148 1.0976624134960768 9 0.16341587839695892 0.9321830665427595 >10 0.7942701917166204 8.91307186540667 >50 0.04633850309077048 2.0085023702182037 >100 0.02101343100981928 2.410999418091594 >500 7.471532071057688E-4 0.3104179370493746 >1k 2.2414596213164625E-4 0.43401684104602395 >5k 7.471532071054875E-5 0.27513779012301137 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5810 0.275007975704631 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4673 0.2211897195297316 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3313 0.15681607977787307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 9.466711728214493E-5 0.0 3 0.0 0.0 0.0 9.466711728214493E-5 0.0 4 0.0 0.0 0.0 9.466711728214493E-5 0.0 5 0.0 0.0 0.0 9.466711728214493E-5 0.0 6 0.0 0.0 0.0 9.466711728214493E-5 4.733355864107246E-5 7 0.0 0.0 0.0 9.466711728214493E-5 4.733355864107246E-5 8 0.0 0.0 0.0 9.466711728214493E-5 4.733355864107246E-5 9 0.0 0.0 0.0 1.420006759232174E-4 1.420006759232174E-4 10-11 0.0 0.0 0.0 1.8933423456428985E-4 1.420006759232174E-4 12-13 0.0 0.0 0.0 1.8933423456428985E-4 2.603345725258985E-4 14-15 0.0 0.0 0.0 2.3666779320536232E-4 3.0766813116697104E-4 16-17 0.0 0.0 0.0 3.5500168980804345E-4 3.5500168980804345E-4 18-19 4.733355864107246E-5 0.0 0.0 4.4966880709018843E-4 4.7333558641072463E-4 20-21 4.733355864107246E-5 0.0 0.0 6.153362623339421E-4 4.7333558641072463E-4 22-23 4.733355864107246E-5 0.0 0.0 9.466711728214493E-4 4.970023657312609E-4 24-25 4.733355864107246E-5 0.0 0.0 0.0012543393039884203 5.206691450517972E-4 26-27 4.733355864107246E-5 0.0 0.0 0.0017513416697196812 5.206691450517972E-4 28-29 4.733355864107246E-5 0.0 0.0 0.0033370158841956087 5.206691450517972E-4 30-31 4.733355864107246E-5 0.0 0.0 0.007975704631020712 5.206691450517972E-4 32-33 4.733355864107246E-5 0.0 0.0 0.018460087870018262 6.153362623339421E-4 34-35 4.733355864107246E-5 0.0 0.0 0.036304839477702584 6.626698209750145E-4 36-37 4.733355864107246E-5 0.0 0.0 0.06534397770400054 6.626698209750145E-4 38-39 4.733355864107246E-5 0.0 0.0 0.10690284219086216 7.10003379616087E-4 40-41 4.733355864107246E-5 0.0 0.0 0.15007104767152024 7.10003379616087E-4 42-43 4.733355864107246E-5 0.0 0.0 0.1971206049607463 7.336701589366233E-4 44-45 4.733355864107246E-5 0.0 0.0 0.2535658736402252 7.573369382571594E-4 46-47 9.466711728214493E-5 0.0 0.0 0.31867318355102037 7.573369382571594E-4 48-49 9.466711728214493E-5 0.0 0.0 0.3856265022488174 8.046704968982319E-4 50-51 9.466711728214493E-5 0.0 0.0 0.4557985029342073 8.046704968982319E-4 52-53 9.466711728214493E-5 0.0 0.0 0.524195495170557 8.520040555393044E-4 54-55 9.466711728214493E-5 0.0 0.0 0.6021775330317238 8.993376141803769E-4 56-57 9.466711728214493E-5 0.0 0.0 0.6777928929608371 8.993376141803769E-4 58-59 9.466711728214493E-5 0.0 0.0 0.7333861575847768 8.993376141803769E-4 60-61 9.466711728214493E-5 0.0 0.0 0.7788737074388474 8.993376141803769E-4 62-63 1.420006759232174E-4 0.0 0.0 0.8182078946695786 8.993376141803769E-4 64-65 1.420006759232174E-4 0.0 0.0 0.8653047855174457 8.993376141803769E-4 66-67 1.420006759232174E-4 0.0 0.0 0.9077866543978084 8.993376141803769E-4 68-69 1.420006759232174E-4 0.0 0.0 0.9591435655233719 8.993376141803769E-4 70-71 1.420006759232174E-4 0.0 0.0 1.0383089423505656 9.466711728214493E-4 72-73 1.6566745524375362E-4 0.0 0.0 1.1433421089751055 9.466711728214493E-4 74-75 1.8933423456428985E-4 0.0 0.0 1.2869047923334782 9.466711728214493E-4 76-77 1.8933423456428985E-4 0.0 0.0 1.4718133391648278 9.466711728214493E-4 78-79 1.8933423456428985E-4 0.0 0.0 1.707984130004459 9.466711728214493E-4 80-81 1.8933423456428985E-4 0.0 0.0 1.9899974723879685 9.703379521419855E-4 82-83 1.8933423456428985E-4 0.0 0.0 2.2723421496819656 9.940047314625218E-4 84-85 2.1300101388482608E-4 4.733355864107246E-5 0.0 2.5200149952713775 9.940047314625218E-4 86-87 2.3666779320536232E-4 9.466711728214493E-5 0.0 2.751736431598748 9.940047314625218E-4 88-89 2.3666779320536232E-4 9.466711728214493E-5 0.0 2.9802628527178454 0.0010413382901035943 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4655 0.0 38.012924 1 GTATCAA 7220 0.0 33.85418 1 ATCAACG 8520 0.0 27.847328 3 TCAACGC 8665 0.0 27.326897 4 TATCAAC 8845 0.0 26.990486 2 CAACGCA 8825 0.0 26.831451 5 AACGCAG 9135 0.0 25.903736 6 TACATGG 7345 0.0 24.73016 2 GTACATG 7690 0.0 24.483093 1 ACATGGG 7345 0.0 22.733515 3 ACGCAGA 10490 0.0 22.557735 7 CGCAGAG 10505 0.0 22.390907 8 GCAGAGT 11345 0.0 19.818972 9 GAGTACT 5960 0.0 18.981573 12-13 CATGGGG 3330 0.0 18.697556 4 AGAGTAC 9920 0.0 18.009216 10-11 GTACTTT 6265 0.0 17.530813 14-15 AGTACTT 6305 0.0 17.456978 12-13 CATGGGA 4650 0.0 17.345959 4 TACACTG 1965 0.0 17.283237 5 >>END_MODULE